This article presents the experience ofPhysician-in-charge CHEN Ying-yi in treating maternal-fetal ABO incompatibility from four aspects:cause and pathogenesis ofthe disease, treatment thought, special prescription for the disease alone, and treatment experience.Mrs.CHEN Ying-yi considers the damp, heat and stasis pathogen as key factors ofthe disease, and the deficiency ofthe spleen and the kidney as the fundamental reasons.The principle oftreatment is eliminating pathogenic factors and supporting healthy energy, and treating the disease and preventing abortion.Her empirical prescription of‘lianhuang tang(decoction oflotus receptacle and prepared rhubarb )', modified in accordance with the symptoms, has obtained very good curative effect for the treatment ofthis disease.

Objective To explore the relationship between thrombocytosis and gastric cancer at the points of clinicopathological stage and prognosis.Methods The clinical data of 156 gastric cancer patients from January 2005 to January 2007 were retrospectively analyzed,and the status of patients was obtained through telephone calls.The frequency of patients with thrombocytosis in different clinicopathological stages was analyzed,and comparison of survival time was made between normal PLT groups and thrombocytosis groups.Results 20.5％ patients with gastric cancer were accompanied with thrombocytosis in 156 cases,and the frequency of patients with thrombocytosis in Ⅰ,Ⅱ,Ⅲ,Ⅳ stage were 4.8％,21.7％,24.4％,30.4％,and the difference was statistically significante (x2 =8.768,P =0.003).Patients with thrombocytosis had a shorter survival time than those with normal platelet counts (one year survival rate:59.4％ vs 81.5％,x2 =6.984,P =0.008 ; three years survival rate:34.4％ vs 63.7％,x2 =8.968,P =0.003).Conclusion In gastric cancer,thrombocytosis is associated with TNM stage.The gastric cancer patients with thrombocytosis have poorer prognosis than those without thrombocytosis.

Objective To analyze the clinical characteristics of male patients with systemic lupus ery thematosus in a cohort of Chinese patients.Methods 325 lupus nephritis patients (38 male and 287 female patients) admitted to our hospital from January 1997 to December 2006 were summarized in this cohort.The difference in manifestations and laboratory features were analyzed between male and female lupus nephritis patients.Results ① Male lupus nephritis patients had more frequent episodes of serositis (P＜0.05),while fe male lupus nephritis patients more frequently had arthralgia,malar rash,oral ulcers and neuropsychiatric lu pus.② The kidney was involved with early stage of the disease in male patients.The rate of misdiagnosis was higher in male lupus nephritis patients than that of the female patients.③ The prevalence of ANA positivity and thrombocytopenia was higher in male patients than that of the female patients.The prevanee of positive anti-ENA antibody,anti-Sin antibody,anemia and hypergammaglobulinemia was lower in male patients than those of female patients.No difference was found in the presence of positive anti-RNP antibody,hypocom plementemia,elevated erythrocyte sedimentation rate and leucoeytopenia between the two groups.Conclusion The clinical pictures of male systemic lupus erythematosus patients are atypical except for kidney involvement.The kidney involvement of male lupus patients is characterized by the early onset,rapid progression and high rate of misdiagnosis.Physicians should familiar with these differences.Early diagnosis and appropriate treat ment are essential for controlling disease progress.

Objective To investigate the changes and roles of the long non-coding RNA (IncRNA)during the reprogramming of human induced pluripotent stem cells. Methods Agilent Human lncRNA (4 × 180K) chip was used to check the expression of lncRNA in somatic cells, induced pluripotent stem cells and embryonic stem cells. Compared with differentially expressed lncRNA in somatic cells and induced pluripotent stem cells, lncRNA was selected that may play an important role during the reprogramming of human pluripotent stem cells. Results The lncRNA expression profiles in induced pluripotent stem cells were similar to embryonic stem cells, but were different from the somatic cells. A total of 3 156 differentially expressed lncRNAs were found between stem cells and somatic cells by cluster analysis, and 222 differentially expressed lncRNAs were found during the reprogramming process of human pluripotent stem cells by biological analysis. Conclusion lncRNA may play an important role in reprogramming process of human pluripotent stem.

Objective To explore the changes of urinary Trypsinogen Activation Peptide (TAP) levels in patients with acute pancreatitis (AP).Methods We observed the association between urinary TAP concentration and severity of acute pancreatitis with competitive enzyme-linked immunosorbent assay(ELISA). Urine samples were collected for TAP concentration at admission,24,48,and 72 h from 57 patients with AP who presented within 48 h of the onset of symptoms and from 11 control patients. Results The median urinary TAP at admission for severe acute pancreatitis(SAP) (98 nmol/L) was signficantly higher than the median for both mild pancreatitis (22 nmol/L,P0.05).The peak median urinary TAP in severe eroup was seen at admission.In the following days,TAP concentrations decreased graduallg.Conclusion Breakthrough activation of trypsinogen in pancreatic interstitial and blood may be the key event in the development of SAP.

Objective To investigate the morphological characteristics of the intervertebral foramina and their alterations in the process of disc height loss in low lumbar spine in order to provide basic data for the diagnosis and treatment of foraminal stenosis. Methods Eight fresh cadaveric lumbar spines of L3 to S1 were obtained for study, which were cleaned off their surrounding muscles and kept the ligaments, annulus fibrous and nerve roots attached. The morphological characteristics of foramina of L4,5 and L5S1 and their anatomic relationships with the nerve roots were observed with and without nucleus pulposus in situ under different loading conditions (0, 300, 500 N). The foraminal dimensions including heights, maximal widths and minimal widths were measured, and the disc heights were measured on lateral radiograph under above loading conditions as well. Results Without any loading, the foramina were inverted teardrop shaped, the foramina of L4,5 were similar to that of L5S1. The nerve roots were located in the upper part of the foramina, and passed across the foramina obliquely. With 500 N loading, the shapes of the foramina and the relationships with the nerve roots were found to be changed little. After removal of nucleus pulposus and more loading was applied, the foramina became stenotic gradually and the nerve roots were pushed against the superior pedicle. The foraminal heights, maximal widths and the anterior, posterior disc heights became narrower, and were significantly different from those under no loading condition (P

Antiarrhythmic effect of isoliensinine (IL) was studied on experimental arrhythmic mo-dels induced by coronary artery ligation and 4 different arrhythmogenic drugs in comparison with quinidine(Qu). Results of the study showed that the antiarrhythmic potency of IL was stronger than that of Qu atthe same dosage. The effects of IL on fast and slow response action potentials of myocardium were ob-served in guinea pig papillary muscles by standard microelectrode technique, which showed that IL couldreduce APA and Vmax and shorten the APD50. The results suggested that the antiarrhythmic mechanismof IL is related to its non-specific inhibition of the currents of Na+ and Ca2+.

Objective To investigate the changes of peroxynitrite(ONOO-)in acute myocardial infarction during ischemia-reper fusion.Methods Thirty patients with actute myocardial infarction(single coronary arterial stenosis of left anterior descending in proximal-middle segment) and twenty-four healthy cases were enrolled as the control in our research.Peripheral blood were taken at the time of 24 h and 72 h after primary percutaneous coronary intervention.The patients were further divided into 2 groups according to their serum cTnI level(mild myocardium injury group with cTnI 0.05).Conclusion Plasma peroxynitrite(ONOO-) is related to myocardium injury during ischemia-reperfusion in actue myocardial infarction which suggest that peroxynitrite may aggravate myocardium injury in acute myocardial infarction during ischemia-reperfusion.

ObjectiveTo assess the effects of dopamine,dobutamine and norepinephrine on the P(g-a)CO2 and superior mesenteric blood flow in septic shock.MethodsRabbit septic shock model was established by challenging with intravenous injection of lipopolysaccharides from Escherichia coil(2 mg/kg).The rabbits with septic shock were randomly assigned to 3 groups-dopamine group(n = 8),dobutamine group(n = 8) and norepinephrine group(n = 8).Apart from volume resuscitation with normal saline solution [20 ml/(kg· h)]，dopamine[5μg/(kg·min)],dobutamine[(5μg/(kg·min)]and norepinephrine [(1μg/(kg·min)]were infused in dopamine group,dobutamine group and norepinephrine group respeclively.Cardiac index(CI) and superior mesenteric blood flow index(SMBFI) were continuously monitored by doppler flowrneter.Gastric mucosal PCO2 was evaluated by gas tonometry every 10 min.Arterial and venous blood gas analyses and lactate levels were measured every 1 h.ResultsMAP,CI,and SMBFI significandy decreased and P(g-a) CO2 increased after lipopolysaccharides infusion in three groups.After 2-hour treatment,MAP in norepinephrine group[(70 +3) mm Hg]was higher than that of dopamine group[(66±4) mm Hg]and dobutamine group[(65±4) mm Hg](P ＜0.05).SMBFI in norepinephrine group [(18.7±2.9) ml/(kg·min)]was higher than that of dopamine group[(16.2±1.6) ml/(kg·min)]and dobutamine group[(15.8±1.9) ml/(kg·min)](P＜0.05).P(g-a) CO2 in norepinephrine group [(30±6) mm Hg]was lower than that of dobutamine group[(23±5)mm Hg](P＜0.05).Condnsion As an adjuvant therapy of volume resuscitation,norepinephrine is more effective than low dose dopamine and dobutamine in improving splanchnic perfusion.

Objective:To study the data collecting from mental assistance hotline for SARS in Beijing to find the psychological changes of the local citizens from the end of April to the first part of June, 2003 Methods:Quantitative statistic and qualitative method were used to analyse the data got from the hotline Result:The data showed there were three psychological phases: panic, irritancy and recovering phases among Beijing citizens during the SARS epidemic The importance of setting a national disasters and crisis intervention system was also pointed out at authors at the end of this paper