1.Studies on the Mechanisms of the Apoptotic Cell Death of Target Cells Mediated by Human Monocytes
Chinese Journal of Cancer Biotherapy 1994;0(01):-
The mechanisms of cytotoxicity mediated by pokeweed-mitogen (PWM)-activated human peripheral blood monocytes was investigated in this study. By DNA electrophoresis and propidium iodide ( Pl) -DNA staining flow cytometry, the study demonstrated that apoptotic cell death of target U937 cells and Raji cells was induced in lectin-depen-dent monocyte-mediated cytotoxicity ( LDMC) . The LDMC-mediated DNA fragmentation in U937 cells could be completly inhibited by anti-TNF-?monoclonal antibodies McAbs, instead of the addition of monosaccharide (N-acetyl glucosamine, Glc NAC) . In contrast, Glc NAC inhibited the DNA fragmentation in Raji cells induced by PWM-monocytes while the anti-TNF-a McAbs had no effect. By flow cytometry, we also demonstrated that PWM could bind with tumor cells as well as with monocytes, at the stimulation of the production of nitric oxide ( NO-) from monocytes. This NO-production was enhanced in the presence of target cells, but the enhacement was abolished by the treatment of GlcNAc.The presence of Lectin-like receptors on the surface of monocytes and tumor cells may bring the effector target cells together, thus facilitating the induction of apoptosis in target cells by triggering the production of cytolytic factors and the modification of target cell surface antigens.
2.Utilization Analysis of Antifungal Medicines in 34 Hospitals in Wuhan Area during the Period of 2012-2014
China Pharmacist 2016;19(5):938-941
Objective:To evaluate the present situation and trend of antifungal medicines used in 34 hospitals in Wuhan area. Methods:The sales volume,market share,DDDs and DDC of antifungal medicines in 34 hospitals in Wuhan area during 2012 and 2014 were statistically analyzed. Results:The consumption sum and growth rate of the medicines were increased year by year, and those of pyrrolic medicines were the highest. The sales volume of voriconazole ranked the first. The DDDs of clotrimazole vaginal tablets and nifuratel-nysfungin suppositories ranked the top two. Conclusion:The consumption of antifungal medicines in Wuhan area has been increased greatly and the consumption structure is relatively stable. The utilization evaluation of antifungal medicines should be enhanced. Antifungal medicines should be rationally used to prevent or slow down the spread of drug resistant bacteria.
3.Effect of propofol on expression of c-fos mRNA in hippocampus in rats with visceral pain
Chinese Journal of Anesthesiology 2012;(11):1314-1316
Objective To investigate the effect of propofol on the expression of c-fos mRNA in hippocampus in rats with visceral pain (VP).Methods Thirty male Sprague-Dawley rats,weighing 180-240 g,were randomly divided into 3 groups (n =10 each):VP group,propofol 7.5 mg/kg group (group P1) and propofol 75.0 mg/kg group (group P2).0.9% normal saline was injected intravenously via the caudal vein in group VP.Propofol 7.5 and 75.0 mg/kg were injected intravenously via the caudal vein in groups P1 and P2,respectively.VP was produced by colorectal distension in anesthetized rats.The threshold of VP was assessed by the intra-balloon pressure which was limited to 100 mm Hg to avoid damage to intestine before and after administration.The rats were sacrificed after measurement of the pain threshold,their brains were removed and hippocampi were isolated for determination of the expression of c-fos mRNA by RT-PCR.Results Compared with group VP,the threshold of VP was significantly increased and the expression of c-fos mRNA in hippocampus was down-regulated in groups P1 and P2 (P < 0.05).The threshold of VP was significantly higher and the expression of c-fos mRNA in hippocampus was lower in group P2 than in group P1 (P < 0.05).Conclusion Propofol can reduce VP through down-regulating the expression of c-fos mRNA in hippocampus in rats.
4.Fluid therapy for acute pancreatitis:timing of resuscitation,type of fluid,and monitoring methods
Journal of Clinical Hepatology 2017;33(1):12-16
Microcirculation disturbance and ischemia of the pancreas are important pathophysiological changes in the onset of acute pancrea-titis,and organ hypoperfusion is a risk factor for poor prognosis.During the first 12 to 24 hours after admission,fluid resuscitation is the most important medical treatment for the early stage of acute pancreatitis,and timely and rational fluid infusion helps to improve prognosis. However,there lacks high -quality clinical evidence for type of fluid,infusion speed,and monitoring methods,and more randomized con-trolled trials are needed.
5.Effects of c-Jun N-terminal Kinase on Expression of Interleukin-10 in Keratinocytes Induced by Ultraviolet A
Li AN ; Qian GAO ; Guoqing DONG
Journal of Environment and Health 1992;0(04):-
Objective To explore the effect of c-Jun N-terminal kinase(JNK)on the expression of interleukin-10(IL-10) in keratinocytes induced by ultraviolet A(UVA).Methods The HaCaT cells in cultured were either sham irradiated(control) or exposured to 2.4 J/cm2 UVA radiation.The cells were collected at 0-48 h after irradiation,and JNK levels in cells were detected with the immunofluorescence.HaCaT cells were treated with SP600125(a JNK inhibitor) before irradiation,then cells and suspended medium were collected at each time-point after irradiation,and the expression of IL-10 mRNA and protein were determined by RT-PCR and ELISA.Results Compared with control cells,irradiated cells had increased levels of phospho-JNK throughout the entire 48 h following irradiation(P
6.Update on myocardial bridging.
Chinese Journal of Cardiology 2006;34(5):474-476
7.The application of standardization to oral medicine.
Chinese Journal of Stomatology 2005;40(6):518-520
8.EFFECT OF BEE POLLEN AND ITS ALCOHOL EXTRACT ON LIP1D PEROXIDATION IN VIVO AND IN VITRO
Bochu QIAN ; Yun QIAN ; Xingxing ZANG ; Baofeng QI ; Wen DONG
Acta Nutrimenta Sinica 1956;0(04):-
The effect of bee pollen of Brassica campestris L. and its alcohol extract on lipid peroxidation was observed in vivo and in vitro.The results showed that the production of lipid peroxides in normal liver hotnogenate of mice and elevation of production of lipid peroxides induced by cysteine and FeSO4 in homogenate were found to be inhibited significantly by in vitro addition of alcohol extract of bee pollen.The elevation of lipid peroxides in serum and liver in adult mice induced by alloxan 75 mg/kg(iv)or by administration of peroxidized corn oil 0.2 ml/mouse was markedly inhibited by oral administration of bee pollen (10 g? kg-1?d-1)for 20 days as compared with respective control groups.The level of lipid peroxide in geriatric mice was also markedly lowered by oral administration of bee pollen (10 g?kg-1?d-1)for 3 months as compared to non-treated geriatric mice.Based on the above in vitro and in vivo experimental results, it may be suggested that bee pollen and its alcohol extract protect tissues against destruction by lipid peroxides.
9.Detection of the IDH1 gene mutation in acute myeloid leukemia by PCR-high resolution melting analysis.
Jiang LIN ; Jun QIAN ; Dong-Ming YAO
Chinese Journal of Hematology 2011;32(7):484-486
Adolescent
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Adult
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Aged
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Aged, 80 and over
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Female
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Humans
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Isocitrate Dehydrogenase
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genetics
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Leukemia, Myeloid, Acute
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genetics
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Male
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Middle Aged
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Mutation
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Polymerase Chain Reaction
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methods
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Young Adult
10.Six cases of occupational acute 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride poisoning.
Qian ZHOU ; Xiang-dong JIAN ; Wei ZHANG
Chinese Journal of Industrial Hygiene and Occupational Diseases 2011;29(5):348-348
Adult
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Carbodiimides
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poisoning
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Humans
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Male
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Methylamines
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poisoning
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Occupational Exposure
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Young Adult