The activating effect of NDV-L on murine PEM? in vitro and the anti-metastatic effect of NDV-L-PEM? in vitro against B16 melonoma were investigated. The results showed that NDV-L could not only activate murine PEM? morphologically , but also enhance the TNF-? gene expression. If the activated NDV-L PEM? were adoptively transferred in the B16 melanoma bearing mice, significant inhibiting effect against pulmonary metastases could be exhibited.

Objective To explore the effect of atorvastatin in the treatment of nonalcoholic fatty liver disease and its clinical application value.Methods 110 patients with nonalcoholic fatty liver disease were randomly divided into observation group and control group,55 cases in each group.The control group was given compound dichloroace-tate diisopropylamine orally,the observation group was given atorvastatin therapy.The effects of the two groups were recorded.Results In the observation group after treatment,the alanine aminotransferase was (39.78 ±3.45) U/L, aspartate aminotransferase was (29.17 ±3.17) U/L,gamma glutamine transpeptidase was (54.28 ±4.11) U/L.In the control group after treatment,the alanine aminotransferase was (52.78 ±6.81) U/L,aspartate aminotransferase was (39.96 ±6.21)U/L,gamma glutamine transpeptidase was (68.69 ±8.31)U/L,there were statistically signifi-cant differences between the two groups(t=12.6290,11.4770,11.5273,all P<0.05).In the observation group after treatment,the triglycerides was (1.66 ±0.32)mmol/L,total cholesterol was (3.27 ±0.37)mmol/L,low density lipo-protein was (1.94 ±0.45)mmol/L.In the control group after treatment,the triglycerides was (2.38 ±0.92)mmol/L,total cholesterol was (5.74 ±1.49)mmol/L,low density lipoprotein was (3.46 ±1.17)mmol/L,there were statis-tically significant differences between the two groups(t=5.4818,11.9316,8.9925,all P<0.05).In the observation group after treatment,the ultrasonic score was (1.33 ±0.12),liver/spleen CT ratio was (0.33 ±0.08).In the con-trol group after treatment,the ultrasonic score was (1.78 ±0.35),liver/spleen CT ratio was (0.47 ±0.21),there were statistically significant differences between the two groups(t =9.0197,4.6202,all P <0.05).Conclusion Atorvastatin in the treatment of nonalcoholic fatty liver disease can effectively improve the liver function,reduce blood lipid concentration,minor adverse reactions,and it is worth popularizing in clinical use.

Objective To investigate the effect of propofol on the expression of c-fos mRNA in hippocampus in rats with visceral pain (VP).Methods Thirty male Sprague-Dawley rats,weighing 180-240 g,were randomly divided into 3 groups (n =10 each):VP group,propofol 7.5 mg/kg group (group P1) and propofol 75.0 mg/kg group (group P2).0.9％ normal saline was injected intravenously via the caudal vein in group VP.Propofol 7.5 and 75.0 mg/kg were injected intravenously via the caudal vein in groups P1 and P2,respectively.VP was produced by colorectal distension in anesthetized rats.The threshold of VP was assessed by the intra-balloon pressure which was limited to 100 mm Hg to avoid damage to intestine before and after administration.The rats were sacrificed after measurement of the pain threshold,their brains were removed and hippocampi were isolated for determination of the expression of c-fos mRNA by RT-PCR.Results Compared with group VP,the threshold of VP was significantly increased and the expression of c-fos mRNA in hippocampus was down-regulated in groups P1 and P2 (P ＜ 0.05).The threshold of VP was significantly higher and the expression of c-fos mRNA in hippocampus was lower in group P2 than in group P1 (P ＜ 0.05).Conclusion Propofol can reduce VP through down-regulating the expression of c-fos mRNA in hippocampus in rats.

Objective To explore the effects of hypoxia on expression of signal transduction and activators of transcription (STAT3) gene and proliferation of rat pulmonary arterial smooth muscle cells (PASMCs). Methods RT PCR and western blot were used to assess the levels of STAT3 mRNA and tyrosine phosphorylated STAT3 protein after primarily cultured rat PASMCs were exposed to hypoxia for 2h, 6h, 12h, 24h respectively. 3 H TdR incorporation was used to observe the cell proliferation. Results The expression of STAT3 mRNA was increased after exposure of PASMCs to hypoxia for 2h, and peaked when exposed for 6h, then declined when exposed for 12h, but still higher than that in PASMCs when exposed to normal oxygentension. The level of tyrosine phosphorylated STAT3 was increased when exposed for 6h,peaked after 12h and delined after 24h in PASMCs exposed to hypoxia. With prolongation of time of hypoxia, 3 H TdR incorporation in PASMCs was significantly higher, and reached a maximum after 24h. Conclusion The results suggested that hypoxia could induce the expression of STAT3 mRNA and activation of STAT3 protein in PASMCs, implying that STAT3 gene might play an important role in the early stage of hypoxia induced PASMCs proliferation

Objective To study the diagnosis and treatment of the severe pulmonary infection in the patients after kidney transplantation. Methods The clinical data of 26 patients with severe pulmonary infection following kidney transplantation were analyzed retrospectively. Results Microorganisms were isolated and identified in 22 patients out of 26 kidney transplantation patients with severe pulmonary infection. The main etiological pathogens according to their frequency and type were: bacteria (15 cases, including Escherichia coli, Aerobacter cloacae, Klebsiella fredlanderi, Enterococcus faecalis, Staphylococcus epidermidis, etc.), fungi (12 cases, Fermentum, Blastomyces albicans, Candida tropicalis, Aspergillus, etc.), and cytomegalovirus (10 cases). 46.15% (12/26) of patients were infected with one kind of microorganism, and 53.85% (14/26) of patients were mixed infection. In 73.1% (19/26) of patients the pulmonary infection occurred during 1-6 months after renal transplantation. Among 26 patients, 12 developed ARDS, and 4 patients gave up therapies due to high expenses. With energetic treatment, 18 patients (81.82%) were cured and 4 died. Conclusions Intensive care and active measures should be given in the treatment of severe pulmonary infection after kidney transplantation. Early diagnosis, administration of broad-spectrum and combined use of antibiotics, the early identification of pathogens, enforcement in systemic support, including correction of immunosuppression, the timely use of mechanical ventilation to correct hypoxia, are the key treatment strategies for a successful result.

Objective To explore the multiple pelvic fractures projection methods and value. Methods 50 cases of pelvic trauma patients were divided into two groups for control photography, and underwent X-ray examination with different projection angles. Results The display effect of the image of the image by 35? projection was better than that of the control group. Conclusion 35? projection results in the images satisfying clinical requirements.

Objective To find out about rural doctors' knowledge on the basic facts about AIDS and the spread of HIV via illegal blood collection and supply and explore viable intervention methods in AIDS prevention and treatment in rural areas. Methods All the rural doctors in a certain township where the epidemic of HTV being spread via blood collection and supply was pretty serious were selected for the survey and data were collected by combining qualitative and quantitative studies. Results The survey of 33 doctors was effective( 100% ) ; above 95% had a correct understanding of the spread of AIDS through sex and blood as well as its contagiousness; 50% did not possess the knowledge that HIV could be spread to the baby by the mother but could not be spread through saliva, sweat, mosquito bites or sharing of bathtubs or toilets; above 80% knew about the objective of the blood organizers in their villages, the peak time of blood selling by the villagers, and the risks of diseases being spread via blood, and were opposed to blood selling; 27.3% once in a while used disposable syringes, and 15.2% discarded or sold used disposable syringes. Conclusion There are severe lurking perils of iatrogenic cross infection in rural areas. Rural doctors are in dire need of formal training in AIDS prevention and treatment knowledge and could be expected to become the key force in AIDS prevention and treatment in rural areas.

Objective To assess the effect of immunosuppression conversion from cyclosporine(CsA) to tacrolimus(FK506) on progression of chronic allograft nephropathy(CAN).Methods A retrospective study was performed in 36 cyclosporine treated renal transplant recipients.Patients were included if they were biopsy-proven CAN and they were converted from cyclosporine to tacrolimus due to CAN.The FK506 dose was 1/50 to 1/100 of the cyclosporine's and the dose was adjusted according to the trough level of tacrolimus,body weight of the patient and the situation of CAN.Other immunosuppressive agents kept unchanging.Serum creatinine and FK506 trough level were monitored after conversion.Fasting blood triglyceride,total cholesterol and glucose were determined at baseline and 6th month after conversion to tacrolimus.Results After switching over to FK506 for 6 months,there was a significant improvement in function of renal allograf(P

Rat lung microvascular endothelial cells (LMVEC) was cocultured with rat pulmonary arterial smooth muscle cells (PASMC), and the cultures were randomly divided into 4 groups: normal homotypic group (NH), normal coculture group (NC), lipopolysaccharide homotypic group (LH), LPS coculture group (LC). The cell cycles were analysed with flow cytometry. The results showed that after the treatment of 100ng/ml LPS for 16h, the number of cells in G 1 phase was decreased in both homotypic and coculture of LMVEC and PASMC, while that in S+G 2/M were increased. The number of cells in G 1 phase was larger in LC than LH, but the number of cells at S+G 2/M phase was less in LC than LH. Compared with normal group, the unmber of LMVEC at S phase was increased by homotypic and coculture after the treatment with LPS. It is concluded that LPS could effect the cell cycle and accelerate the cell division, and there is a complicated regulatory mechanism in cell proliferation between LMVEC and PASMC.

Objective To compare the osseo-induction and biodegradation performances of three types of injectable and degradable calcium phosphate cement (CPC) so as to find out a better bone substitute. Methods Three types of injectable and degradable CPC were respectively implanted into the bilateral tibias of 24 New Zealand rabbits: pure CPC (Group A), CPC added with Zinc and Strontiumions (Group B), and CPC with composite rhBMP-2 (Group C) . Their systematic and local reactions in implanted region were closely observed. The degra- dation and osseo-induction performances were compared macroscopically, microscopically and by CT scan to find out the one that could best meet clinical needs. Tissue slices were sampled and photographed four, eight and 16 weeks after operation. Five photographs were selected in each group and at each time points for computer software (Image Pro Plus 5.1) processing to calculate the percentages of bone in the images of postoperative slices. Results In Groups A and B, new bone was found to form slowly and little by little, and the ossification was not synchronous with the material degradation. In Group C, however, new bone was observed to form early and massively, and the os- sification was almost synchronous with the material degradation. In Groups A, B and C, the percentage of bone in the images of postoperative slices was (41.7?16.6)%, (31. 2?12.2)% and (71.7?21.0)% respectively. The bone percentage in CPC with composite rhBMP-2 was significantly higher than that in the other two types of CPC (P＜0.01 ). Conclusion The injectable and degradable CPC with composite rhBMP-2 is more suitable for clinical use, because it can induce early new bone formation and synchronous biodegradation.