OBJECTIVEThis study aimed at investigating effects of exogenous interleukine-10 (IL-10) on IL-6 and intercellular adhesion molecular (ICAM-1) expression in inflamed gingival tissue.

METHODSThe expression of IL-6 and ICAM-1 was examined using immunohistochemical techniques. Inflammatory gingival tissue treated with IL-10 was taken as the experimental group and the same patient's inflammatory gingival tissue without treatment of IL-10 was included into the control group.

RESULTSIL-6 expression was found mainly in monocytes, macrophages, lymphocytes, endotheliocytes and fibroblasts. The expression of ICAM-1 was found mainly in epithelial cells, monocot-macrophages, lymphocytes, endotheliocytes and fibroblasts. The immunohistochemical optical density (IOD) of the expression of IL-6 and ICAM-1 was detected by using Image-Proplus software, and the results showed that the expression in the experimental group differed significantly from that in the control group.

CONCLUSIONThe exogenous IL-10 may down-regulate IL-6 and ICAM-1 expression in inflammatory gingival tissue.

Adult ; Down-Regulation ; Female ; Gingiva ; metabolism ; Gingivitis ; metabolism ; Humans ; Intercellular Adhesion Molecule-1 ; biosynthesis ; genetics ; Interleukin-10 ; pharmacology ; Interleukin-6 ; biosynthesis ; genetics ; Male ; Middle Aged

ObjectiveTo evaluation the intervention effects of hand hold hands method in brick tea borne fluorosis.MethodFour to six grade pupils were selected in brick tea borne fluorosis areas,and health education knowledge questionnaire investigation was conducted.Drinking tea related health behaviors were also surveyed by the method of questionnaire.Then health education lecture on brick tea borne fluorosis was carried out,and health education material and low fluoride brick tea were provided to the pupils,the students were also requested to tell their parents brick tea related knowledge and supervise them to get rid of harmful drinking tea behavior,and cultivate healthy lifestyle.One year later pupils' health knowledge questionnaire and parents health related behavior questionnaire were carried out once again.The knowledge awareness rates of students before and after the intervention were compared.Health related behavior change rates of the parent between different ages and education levels were compared,and the implementation rates of different brick tea related behaviors before and after the intervention were compared.ResultsAfter 1 year of health education,the student's knowledge awareness rate on brick tea fluorosis was [94.3％ (33/35)],compared with the rate before intervertion [14.3％ (5/35)] increased significantly(X2 =46.111,P ＜ 0.01 ).The overall behavior change rate of health related behavior was 68.6％.After the intervention,the implementation rate of whether drinking tea,drinking overnight tea,drinking boiled for a long time tea decreased(P ＜ 0.01).But the behavior implementation rates of drinking tea with milk,drinking and buying low fluoride brick tea significantly increased(P ＜ 0.05).Implementation rate of whether drinking brick tea did not change significantly(P ＞ 0.05).ConclusionsThrough hand hold hands health education intervention,the knowledge awareness of students on brick tea fluorosis increases,and their parents can actively change bad tea drinking habits,and cultivate healthy lifestyle.

OBJECTIVETo show the trend of the retention change during the circulation of the clasp dislodging and inserting, and to discuss the proper depth of the undercut for a specific clasp. It is also a basic study for the best designing of RPD.

METHODSThe Chatillon force measuring instrument was used to record the variation of the retention of Co-Cr alloy casting Akers clasp when they were dislodging from undercuts of different depths (0.25 mm, 0.50 mm, 0.75 mm) on bicuspid and molar.

RESULTSThe data was analysed of Correlation and Regression by SPSS 10.0 statistic software. The variations of the retention in the circulation are obvious from the different teeth and different undercut depths. It shows an attenuation trend in circulation (P < 0.01). After 400 times dislodging, in bicuspid group, the retention of the clasp used in 0.50 mm undercut became less than the one used in 0.25 mm undercut. While the retention of the clasp used in 0.75 mm decreases most obviously, it values 0.7670 kg. In molar group, the attenuations of the retention at different depths of undercut are similar, the retentions of the clasp still rank as, from great to less, 0.75 mm, 0.50 mm and 0.25 mm, in the depth of undercut.

CONCLUSIONthe proper depth of the undercut for bicuspid is 0.25 mm, and 0.50 mm for molar.

Dental Clasps ; Dental Prosthesis Retention ; Humans

OBJECTIVETo investigate the effect and mechanism of periodontal initial therapy on type 2 diabetes patients with periodontitis.

METHODS15 type 2 diabetes patients with periodontitis were selected. Their body mass index (BMI), sulcus bleeding index (SBI), probing depth (PD), circulating tumor necrosis factor-alpha (TNF-alpha) concentration, and the value of glycosylated hemoglobin (HbA1C), triglycerides (TG) and cholesterol (CHOL) were assessed respectively before and 4-6 weeks after periodontal initial therapy.

RESULTSAfter initial therapy, SBI, PD, circulating TNF-alpha concentration, and the value of HbA1C and TG were reduced significantly (P<0.05), while there were no significant differences in BMI and CHOL value (P>0.05).

CONCLUSIONSPeriodontal initial therapy can effectively reduce HbA1C value in type 2 diabetes patients with periodontitis, possibly through the reduction of circulating TNF-alpha concentration.

Adult ; Aged ; Body Mass Index ; Diabetes Mellitus, Type 2 ; blood ; Female ; Glycated Hemoglobin A ; analysis ; Humans ; Lipids ; blood ; Male ; Middle Aged ; Periodontitis ; blood ; therapy ; Tumor Necrosis Factor-alpha ; analysis

BACKGROUNDLarge discrepancy of the incidence of myocardial bridging (MB) has been reported either among the postmortem studies or among the studies with coronary angiogram. This study was to investigate the prevalence of MB in large number of coronary angiograms and the angiographic characteristics of MB.

METHODSA total of 5525 consecutive patients who underwent first diagnostic coronary angiography from January 2003 to March 2006 in Zhongshan Hospital were enrolled in this study. MB was diagnosed when the angiographical "milking effect", defined as the systolic compression and complete or partly release of the compression in diastole, was seen in the epicardial coronary arteries. Angiography was routinely repeated after intracoronary injection of 200 microg nitroglycerin. The systolic compression and length of MB were compared before and after the administration of nitroglycerin and also before and after stent implantation in patients with significant stenosis in segment proximal to the MB.

RESULTSAmong 5525 patients, MBs were found in a total of 888 patients angiographically with the prevalence of 16.1%. Atherosclerotic lesions were found more often in the segment proximal to the MB with 344/854 (40.3%) patients than in the segment distal to the MB with 47/854 (5.5%) (P < 0.01). The systolic compression ((43.3 +/- 13.7)% at baseline vs (54.2 +/- 14.0)% after nitroglycerine) and the average length ((20.9 +/- 7.5) mm at baseline vs (22.7 +/- 8.0) mm after nitroglycerine) of the MB segment were increased after intracoronary injection of nitroglycerin (both P < 0.01). Stent implantation was performed in 88 patients with significant stenosis in the segment proximal to the MB. The systolic compression and the length of the MB segment were increased after stenting compared with those before stenting (systolic compression, (49.4 +/- 14.6)% at baseline vs (57.3 +/- 12.3)% after stenting, and length of MB, (19.5 +/- 6.1) mm at baseline vs (21.8 +/- 6.3) mm after stenting, P < 0.01).

CONCLUSIONSMB was a frequent finding in coronary angiogram with an incidence of 16.1%. Intracoronary administration of nitroglycerin and stent implantation in the segment proximal to the MB could enhance the systolic compression and the length of the MB angiographically.

Aged ; Coronary Angiography ; methods ; Female ; Humans ; Male ; Middle Aged ; Myocardial Bridging ; diagnosis ; pathology ; Retrospective Studies

Photodynamic therapy (PDT), because of its good targeting, minimal invasion, and safety, is becoming a very active area in cancer prevention and treatment, in which the photosensitizers have proved to be the core element for PDT. We developed a new HPLC method for analyzing porphyrin photosensitizers using Shiseido Capcell PAK C18 (150 mm x 4.6 mm, 5 µm) as the column at 30 °C, methanol-1% aqueous solution of acetic acid as the mobile phase in a flow rate of 1.0 mL · min(-1) in a gradient elution mode, and the detection wavelength at 380 nm. This method, showing good specificity, precision, accuracy and robusty via methodology validations, can be applied to the purity test and assay of porphyrin photosensitizers, and has played a key guide role in the R&D of the new porphyrin photosensitizer--sinoporphyrin sodium.
Chromatography, High Pressure Liquid ; Photochemotherapy ; Photosensitizing Agents ; chemistry ; Porphyrins ; chemistry

OBJECTIVETo evaluate the short-term outcome of patients receiving metal-on-mental hip resurfacing arthroplasty and analyze the factors affecting the result.

METHODSIn 13 patients receiving metal-on-metal hip resurfacing arthroplasties with scheduled follow-up, the treatment outcomes were assessed according to the Harris Hip Score.

RESULTS AND CONCLUSIONThe short-term outcomes of hip resurfacing arthroplasties were encouraging, and intraoperative complications, infections, peripheral nerve palsy, hip dislocations or clinically overt lower limb deep vein thrombosis occurred in none of these cases. All the patients reported complete or major pain relief. Clinical assessment according to the Harris Hip Score revealed an improvement from an average score of 36.69 preoperatively to 89.63 after the surgery. Crutch use lasted for a maximum of 4 weeks postoperatively in these patients, after which the patients were all capable of walking without crutches with full weight-bearing.

Adolescent ; Adult ; Arthroplasty, Replacement, Hip ; methods ; Female ; Femur Head Necrosis ; surgery ; Humans ; Male ; Middle Aged ; Osteoarthritis, Hip ; surgery ; Treatment Outcome ; Young Adult

OBJECTIVETo study the color tolerance of anterior ceramic crown and seek an effective approach for color-matching of oral prostheses.

METHODS30 single maxillary incisor ceramic prostheses and the corresponding nature teeth were measured by a digital camera in a steady environment for image taking and color measurement, which based on the standard recommended by CIE. The color tolerance was analyzed in two groups based on satisfaction and dissatisfaction of prostheses color-matching.

RESULTSThe deltaE of satisfaction group was 2.5503, while the deltaE of dissatisfaction group was 4.0772. There was significant difference between chromatic aberration of satisfaction group and dissatisfaction group (P<0.01). When the lightness and saturation of prosthesis were higher than nature teeth, there was no significant difference between deltaL*, deltab*, deltaC* of satisfaction group and dissatisfaction group (P>0.05). When the lightness and saturation of prosthesis were smaller than nature teeth, there was significant difference between deltaL*, deltab*, deltaC* of satisfaction group and dissatisfaction group (P<0.01). There was significant difference between deltaa*, deltaH of satisfaction group and dissatisfaction group (P<0.05, P<0.01).

CONCLUSIONControl of color difference is the key for shade matching of anterior tooth prosthesis. Applying the color tolerance is beneficial to improving the prosthesis quality.

Ceramics ; Color ; Crowns ; Dental Porcelain ; Humans ; Incisor ; Light ; Spectrophotometry ; Tooth Crown

OBJECTIVETo study the chemical constituents of the roots of Stellera chamaejasme.

METHODThe chemical constituents were separated and purified by chromatographic method after solvent extraction and were identified by spectroscopic analysis.

RESULTTwo phenolic compounds were obtained and determined as stelleranol (1) and umbelliferone-7-O-glucoside (2).

CONCLUSIONCompound 1 was a new compound, and compound 2 was isolated from this plant for the first time.

Magnetic Resonance Spectroscopy ; Molecular Structure ; Phenols ; chemistry ; isolation & purification ; Thymelaeaceae ; chemistry

OBJECTIVETo explore the biological effects of tetracycline on cultured human periodontal ligament fibroblasts (HPDLFs).

METHODSIncreasing concentrations of tetracycline (1, 5, 20, 100, 500, 2500 microg/ml) were added to the medium of cultured HPDLFs, respectively. After co-incubated for 2 days, cell morphology was observed under reverse microscope, meanwhile, cell proliferation activity was assayed using MTT, the total amount of protein was detected with Coumassie Bright Blue method and DNA synthesis was measured by 3H-TdR.

RESULTSOver a concentration range of 1 to 100 microg/ml, cells demonstrated a normal appearance, spindle or fusiform shaped. Moreover, at a concentration range of 20 to 100 microg/ml, tetracycline significantly enhanced the proliferating activity and biosynthesis of HPDLFs (P < 0.01). However, higher concentration (2500 microg/ml) not only changed cell morphology, but also significantly inhibited cellular activity.

CONCLUSIONThe results suggested that proper doses of tetracycline could promote proliferation and biosynthesis of HPDLFs while higher concentrations of tetracycline had cytotoxic effect.

Cells, Cultured ; Fibroblasts ; drug effects ; Humans ; Periodontal Ligament ; cytology ; drug effects ; Tetracycline ; pharmacology