1.Changes of Matrix Metalloproteinase in Serum and Cerebrospinal Fluid of Children with Purulent Meningitis and Those Clinical Significances
xu-sheng, QI ; tong-guo, PAN ; yu-mei, YANG
Journal of Applied Clinical Pediatrics 1992;0(06):-
0.05,respectively).But most inte-restingly,the MMP-9 showed a positive relevance(r=0.686,P
2.Detecting and Analyzing on Mutation of Fibroblost Growth Factor Recepter 3 Gene in A Congenital Achondroplasia Family
yan-mei, HUANG ; li-wei, GUO ; duan, LI ; ying-jie, QI ; bao-sheng, YANG
Journal of Applied Clinical Pediatrics 2006;0(20):-
Objective To detect and analyze the mutation of fibroblast growth factor receptor 3(FGFR3) gene among a family with congenital achondroplasia(ACH).Methods Six blood samples of family member in this pedigree were cellected according to the informed consent process for genetic research,and 2 unralted healthy human blood sample were taken as controls.The mutation at nucleotide position 1 138 on FGFR3 gene was detected by using Polymerase chain reaction and single-strand conformation polymorphism(PCR-SSCP)and polyme-rase chain reaction and restriction endonuclease technology(PCR-RFLP) methods.Results Using PCR-SSCP method firstly,only the proband with ACH and his father in this family had the same abnormal band.The amplified products including 1 138 loci on FGFR3 gene further was analyzed by Sfe Ⅰ digestion,3 fragments including 164 bp,109 bp and 55 bp were detected in the proband and his father again,and the other members in the family and 2 controls just showed 164 bp band.It indicated that just 2 patients (proband and his father) showed heterozygous G→A transition mutation at nucleotide position 1 138 on the FGFR3 gene.The amplified products at 1 138 loci was also detected by MspⅠ digestion,just 1 band was observed in all members in this family and 2 controls.It showed that there was no G→C substitution at nucleotide position 1 138.Conclusions The G→A transition mutation at nucleotide position 1 138 in transmembrane domain of FGFR3 gene may be the main cause of achondroplasia in this family.In this pedigree,the proband showed's father a de novo mutation which was transferred to his child again.
3.Neuroprotective and mechanistic study of GJ-4 on okadaic acid-induced memory impairment in mice
Yang YANG ; Chan-juan SHENG ; Cai-xia ZANG ; Jun-mei SHANG ; Xiu-qi BAO ; Dan ZHANG
Acta Pharmaceutica Sinica 2023;57(12):3628-3636
GJ-4 is crocin enrichments extracted from
4.Effect of Yinao Jieyu Recipe on Brain Derived Neurotrophic Factor of the Limbic System in Post-Stroke Model Rats.
Dong-mei LI ; Qi-sheng TANG ; Rui-zhen ZHAO ; Xiao-li LI ; Ge WANG ; Xin-ke YANG
Chinese Journal of Integrated Traditional and Western Medicine 2015;35(8):988-992
OBJECTIVETo observe the evolutionary tendency of brain derived neurotrophic factor (BDNF) of the limbic system in post-stroke model rats and the intervention effect of Yinao Jieyu Recipe (YJR).
METHODSMale Wistar rats were randomly divided into the normal control group (n =6), the sham-operation group (n =7), the multiple cerebral infarction (MCI) group (n =10), the post-stroke depression (PSD) group (n =10), the Chinese medicine (CM) treatment group (n =10), and the Western medicine (WM) treatment group (n =10) according to random digit table after open-field testing. Rats in the normal control group were routinely fed. 0. 3 mL normal saline was intravenously pushing from the external carotid artery to rats in the sham-operation group, and distilled water administered to them by gastrogavage. Each dose allogenic microthrombi were in vitro pushed to rats in the rest groups from the external carotid artery. The PSD model was duplicated by 21-day chronic unpredictable mild stress (CUMS) and single cage feeding in the PSD group 7 days after surgery. After preparing models rats in the CM group and the WM group were administered with YJR and Nimodipine respectively for 4 successive weeks. Changes of BDNF and the intervention effect of YJR were observed at week 1, 2, and 4 after intervention.
RESULTSImmunohistochemical results of BDNF showed, compared with the normal control group, expression levels of BDNF in the hippocampus, hypothalamus, and amygdala decreased in the MCI group at week 2 and 4 (P <0. 01 , P <0. 05) ; expression levels of BDNF in each part decreased in the PSD group at week 1-4 (P <0.01). Compared with the MCI group, expression levels of BDNF in each part decreased in the PSD group at week 1-4 (P <0. 01). Compared with the PSD group, expression levels of BDNF in each part increased in the CM group at week 1-4 (P <0. 01).
CONCLUSIONBDNF changes existed in post-stroke model rats, and YJR could slow down this progress.
Amygdala ; Animals ; Brain-Derived Neurotrophic Factor ; metabolism ; Depression ; Depressive Disorder ; Drugs, Chinese Herbal ; pharmacology ; therapeutic use ; Hippocampus ; Male ; Models, Animal ; Rats ; Rats, Wistar ; Stroke ; drug therapy
5.Therapeutic observation of cranial suture acupuncture in treating depression
Chun-Qi AI ; Qin-Bo WANG ; Xi WANG ; Yu WANG ; Sheng-Mei CHEN ; Xiong CHEN ; Jue HONG
Journal of Acupuncture and Tuina Science 2018;16(3):161-166
Objective:To observe the clinical efficacy and safety of cranial suture acupuncture plus paroxetine in treating depression,and to discuss the action mechanism of this acupuncture method.Methods:One hundred depression patients were allocated to an observation group and a control group according to the random number table,with 50 cases in each group.The control group was intervened by oral administration of paroxetine tablets,20 mg each time,once a day for successive 6 weeks;the observation group was additionally given cranial suture acupuncture,once a day for 6 weeks.They were scored by Hamilton depression scale-17 (HAMD-17) before the treatment and respectively after 1-week,2-week,4-week and 6-week treatment.The clinical efficacy and safety were also observed.Results:After 6-week treatment,the total effective rate was 94.0% in the observation group versus 78.0% in the control group,and the between-group difference was statistically significant (P<0.01).The HAMD-17 scores respectively after 1-week,2-week,4-week and 6-week treatment were significantly lower than the score before the treatment in the observation group (all P<0.05);the HAMD-17 scores respectively after 2-week,4-week and 6-week treatment were significantly different from the score before the treatment in the control group (all P<0.05).There were significant differences in the HAMD-17 score between the two groups respectively after 4-week and 6-week treatment (both P<0.05).Conclusion:Cranial suture acupuncture plus paroxetine can ease the symptoms of depression,with faster onset and more significant therapeutic efficacy compared with paroxetine alone.
6.Alkaloids from bulbs of Lycoris radiata.
Xia-Mei LIU ; Lei WANG ; Zhi-Qi YIN ; Sheng-Yuan ZHANG ; Sheng OUYANG ; Wen-Cai YE
China Journal of Chinese Materia Medica 2013;38(8):1188-1192
Ten Amaryllidaceae alkaloids were isolated from the bulbs of Lycoris radiata. Their structures were identified as oxovittatine (1), apohaemanthamine (2), 9-O-demethylhomolycorine N-oxide (3), incartine (4), ismine (5), 6-O-methylpretazettine (6), tazettine (7), ungeremine (8), homolycorine (9), and O-methyllycorenine (10) by spectroscopic data analyses. Compound 1 was a new natural product. Compounds 2 and 3 were reported form the genus Lycoris for the first time and compounds 4-6 were isolated form the title plant for the first time.
Alkaloids
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chemistry
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Lycoris
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chemistry
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Plant Extracts
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chemistry
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Plant Roots
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chemistry
7.Association between single nucleotide polymorphism locus rs189037 in the promoter of ATM gene and nasopharyngeal carcinoma susceptibility in Cantonese.
Hong-mei WANG ; Yu-sheng SHI ; Qi-sheng LI ; Ying LIU ; Xiao-kang ZHENG
Journal of Southern Medical University 2011;31(11):1863-1866
OBJECTIVETo evaluate the correlation between single nucleotide polymorphisms (SNP) in the promoter of ataxia-telangiectasia mutated (ATM) gene and the susceptibility of nasopharyngeal carcinoma in Cantonese.
METHODSA total of 176 NPC Cantonese NPC patients and 202 age-matched healthy controls were enrolled in this study. G/A genotyping of the SNP locus rs189037 in the promoter of ATM gene was performed by PCR and direct sequencing of the PCR products.
RESULTSThe genotype frequency of heterozygote G/A was 50% (88/176) and 47.5% (96/202), and that of the homozygote variant A/A was 15.3% (27/176) and 18.3% (37/202) in NPC patients and healthy individuals, respectively. Statistics analysis revealed no evident correlations of the G/A and A/A genotypes to the clinical phenotypes of NPC in either NPC group or healthy control group (OR7equals;1.022, 95%CIequals;0.668-1.564 for G/A+A/A). Analysis after stratification by age and gender found no such correlations either.
CONCLUSIONThe genotype frequencies of SNP locus rs189037 in the promoter of ATM gene are similar between NPC patients and healthy subjects, suggesting that rs189037 is not related to the genetic susceptibility of NPC in Cantonese.
Adult ; Asian Continental Ancestry Group ; genetics ; Ataxia Telangiectasia Mutated Proteins ; Base Sequence ; Carcinoma ; genetics ; Case-Control Studies ; Cell Cycle Proteins ; genetics ; DNA-Binding Proteins ; genetics ; Female ; Genetic Predisposition to Disease ; Genotype ; Humans ; Male ; Middle Aged ; Molecular Sequence Data ; Nasopharyngeal Neoplasms ; genetics ; Polymorphism, Single Nucleotide ; Promoter Regions, Genetic ; Protein-Serine-Threonine Kinases ; genetics ; Tumor Suppressor Proteins ; genetics
8.Study on seed quality test and quality standard of Platycodon grandiflorum.
Qiao-Sheng GUO ; Rong-Mei ZHAO ; Liu LIU ; Qi-Ting DONG ; Zhi-Wen FU
China Journal of Chinese Materia Medica 2007;32(5):377-381
OBJECTIVETo study quality test, and establish quality classification standard of Platycodon grandiflorum.
METHODThe seed quality and planting quality of P. grandiflorum from different producing areas and in different growing years were measured.
RESULT AND CONCLUSIONThe range of seed purity, weight per 1000 seeds, seed moisture content,seed viability were determined and the primary seed quality classification standard was established.
Germination ; physiology ; Plants, Medicinal ; anatomy & histology ; chemistry ; physiology ; Platycodon ; anatomy & histology ; chemistry ; physiology ; Quality Control ; Reference Standards ; Seeds ; anatomy & histology ; chemistry ; physiology ; Water ; analysis ; standards
9.Effects of electroacupuncture on the apoptosis of brain tissue cells and the expression of Caspase-3 in the rats with cerebral-cardiac syndrome.
Sheng-Bing WU ; Guang-Yuan HE ; Mei-Qi ZHOU
Chinese Journal of Integrated Traditional and Western Medicine 2012;32(5):639-642
OBJECTIVETo observe the effects of electroacupuncture (EA) on the apoptosis of brain tissue cells and the expression of Caspase-3 in the rats with cerebral-cardiac syndrome (CCS).
METHODSA total of 70 healthy SD rats were selected. Ten was randomly recruited as the sham-operation group, and the rest were used for CCS model preparation. Thirty successfully modeled rats were divided into the model group, the EA group, and the non-EA group, 10 in each group. The model was prepared using injecting collagenase + heparin into the caudate nucleus. Equal volume of 0.9% sodium chloride injection was injected to rats' caudate nucleus in the sham-operation group. EA was started on the 1st day of modeling. Shuigou (GV26), Fengfu (GV16), Neiguan (PC6), and Xinshu (BL15) were needled in the EA group. Four points in the hips were needled in the non-EA group. The EA needling lasted for 20 min each time, once daily, for 3 successive times. No EA was administered to the sham-operation group or the model group. The apoptosis of brain tissue around the hematoma and the expression of Caspase-3 were detected using TUNEL and immunochemical assay.
RESULTSTUNEL cells could be occasionally seen with fewer Caspase-3 expression in the sham-operation group. More TUNEL positive cells appeared in the tissue around the hematoma of the model group with a large amount of Caspase-3 expression. The TUNEL positive cells and Caspase-3 expression were obviously less in the EA group than in the model group and the non-EA group (P < 0.01).
CONCLUSIONSEA could inhibit the apoptosis of brain tissue cells in CCS rats. Its mechanisms might be associated with down-regulating the Caspase-3 expression of the brain tissue around the hematoma.
Animals ; Apoptosis ; Brain ; metabolism ; Caspase 3 ; metabolism ; Cerebral Hemorrhage ; complications ; metabolism ; pathology ; Electroacupuncture ; Heart Diseases ; etiology ; metabolism ; pathology ; Rats ; Rats, Sprague-Dawley
10.HLA-B*15 gene polymorphism of registered bone marrow from donors in north Chinese Han population typed by PCR-SBT.
Jun QI ; Meng-Li LIU ; Yan ZHANG ; Sheng LIU ; Chun-Mei SHEN
Journal of Experimental Hematology 2008;16(2):429-434
To investigate the characteristics of the allele distribution of HLA-B*15 gene family in Chinese Han population and to study its influence on the selection of clinical transplantation donor, population of a 815 Han in north China from Shaanxi sub-registry of Chinese National Marrow Donor Project was randomly selected and out of them 206 HLA-B*15 positive samples according to the previous known low-resolution typing results were acquired. HLA-B*15 gene polymorphisms of above-mentioned samples and other 17 individuals were analyzed for the first time by polymerase chain reaction sequence-based typing (PCR-SBT) at high-resolution level. The structure differentiation of all HLA-B*15 alleles were analyzed by HLA three-dimensional structure modeling and software Swiss-PdbViewer. The results showed that the distribution of HLA-A, -B, -DRB1 gene of randomly selected 815 samples accorded with Hardy-Weinberg equilibrium and the gene frequency of HLA-B*15 was 0.1379. There were a total of 16 kinds of alleles of HLA-B*15 gene family to be obtained, which belonged to 7 kinds of serologic specificities. HLA-B*1501, B*1511, B*1502 and B*1518 were the major alleles with a frequency of 0.0485, 0.0215, 0.0178 and 0.0160 respectively, and the constituent ratio of their accumulated frequencies was 75.11%. The each frequency of the other 12 kinds of B*15 alleles was lower than 0.0100. Among the homozygote of 10 samples at low/medial-resolution level, there were only 4 samples to be pur sang homozygote of HLA-B*15xx, --at high-resolution level, and all the homozygote were constituted by respective dominating alleles. HLA three-dimensional structure modeling demonstrated that within the same specificity, gentle structure differentiation not only existed, such as B*1501, 1505, 1507, 1525, 1527, 1532 (each RMSD