The concentration of airborne microbes in summer tourism season in Huangshan Scenic Spot was measured by fallen plate method at l4 collection sites. The study on the community structure and distribution characteristics of outdoor airborne microbes was carried out. The results showed that the average concentra- tion of the bacteria, fungi and total microbes in the outdoor environment were 0.41?104 CFU/m3, 0.34? 104 CFU/m3 and 0.75?104 CFU/m3 respectively, and the average percentage of fungi of the total was 45.9%. These indicated that the outdoor air had been polluted by airborne microbes. The preliminary identification was made on the bacteria and fungi at different sampling sites and the results showed that the dominant bac- teria mainly belonged to Bacillus, Staphylococcus, Micrococcus and Pseudomonas, and the average per- centage of the total amount of the bacteria was 37.7%, 17.2%, 10.1% and 9.8% respectively; the dominant fungi belonged to Cladosporium, Mycelia sterilia, Penicillium and Alternaria, and the average percentage of the total amount of the fungi were 40.4%, 35.0%, 5.6% and 4.3% respectively. The results indicated that thedistribution characteristics of air microbes are affected not only by topographical and climatic characteristics, but also by factors such as tourist activities, animals and plants.

Objective To understand the air microorganisms pollution and the community structure and distribution characteristics of outdoor airborne microbes in Hefei. Methods Airborne microbes were sampled by fallen plate method at 10 collection sites in the Hefei city. Results The results showed that the average concentration of the bacteria,fungi and total microbes in the outdoor environment were 5.04?104,3.75?103 and 5.41?104 cfu/m3 respectively and the fungi accounted for 6.92%of the total. The preliminary identification showed that the dominant bacteria mainly were Micrococcus, Bacillus, Pseudomonas and Staphylococcus and the average percentage were 37.3%, 21.0%, 10.3% and 9.5% respectively. Of the five fungi the dominant ones were Aspergillum, Penicillium, Mucor and Rhizopu. The average percentage were 42.0%,36.0%,4.4% and 4.2% respectively. Conclusion The outdoor air of Hefei has been polluted by airborne microbes. The distribution of the airborne microbes has the characteristics of time and space.It is affected not only by the environment and pollution,but also by such factors as human activities,animals and plants.

Objective In order to investigate the interactions between the activities of daily living of children with post poliomyelitis and depression of their parents.Method 40 cases whose children were with post poliomyelitis were tested by SDSCR.Children's activity of daily living were tested with Barthel's index.Result 65％ of parents whose children with post polimyelitis were depression.Their symptoms of depression were mainly moderate level.There are marked difference comparing with the domestic normal analyzing model.The activities of daily living of the post poliomyelitis's children was worse,the older their parent's age was the higher the incidence of depression occurred was.Conclusion We should pay attention to their parents emotion changes when we carry out the community rehabilitation of post poliomyelitis's children,and strive them for positive coperating our rehabilitation training.

By means of correlative analysis of critical care air transportation management between China Air Force and United State Air Force,five areas of importance are discussed in this paper,such as the management model of strategies for air transportation,critical care team personnel composition,professional training mechanism,normalization and standardi-zation of operation process,and intensive application of new technology and equipment.

Objective:To design the teaching method of problem-based learning(PBL) and verify as well as evaluate its effectiveness in the teaching practice of clinical nuclear medicine.Methods:A new teaching method-PBL was introduced on the basis of summarizing the problems and shortcomings in the teaching of clinical nuclear medicine.The students raised questions centering on the typical cases and discussed under the guidance of the teacher by team.Afterward the teaching effectiveness was judged by the results of test and the evaluation of students and teachers.Results:PBL received favorable feed-backs from students and teachers.The results of test had obviously improved in the PBL groups(P

Objective To evaluate the diagnostic value of 99mTc-dimercaptosuccinate acid(DMSA) renal cortical scintigraphy for the identification of distinguishing between upper urinary infection(UUTI) and lower upper urinary infection(LUTI).Methods Two hundred and seventy-five children (111 males,164 females)ranging from 44 days to 15 years old,presented with urinary tract infection underwent 99mTc-DMSA renal cortical scintigraphy.The images were scored as normal (indicating LUTI) and abnormal (indicating acute pyelonephritis or renal scarring).Results Of 275 children with UTI,95 cases had normal images diagnosed as LUTI,41 males,54 females;and 180 cases had abnormal images,70 males,110 females.One hundred and seventy-four cases were diagnosed as acute pyelonephritis,6 cases were diagnosed as renal cortical scars,56 cases were single renal involved and 118 cases were both renal involved,and 22 cases repeatedly underwent renal cortical scanning after therapy.Sixteen of 18 cases with acute pyelonephritis completely recovered normal or obviously ameliorated after 0.5 to 2.0 years,2 cases did not show any improvement after 0.5 to 1.5 years,4 cases with renal scarring,and showed little change on repeated images after 1.0 to 1.5 years.Conclusions The 99mTc-DMSA renal scintigraphy is very useful in differentiating the children with urinary tract infection.It also can be used to determine the extension,degree and nature of UUTI,and might play an important role in the treatment and follow-up observation in children with UUTI.

The tuberous roots of Aconitum carmichaeli are largely used in traditional Chinese medicine and widely grown in Jiangyou, Sichuan, China. During the growth process, this medicinal plant releases a large amount of allelochemicals into soil, which retard the growth and development of near and late crops. Therefore, a pure culture experiment was thus carried out by seed soaking to study the allelopathic effects of extracts from tuberous roots of A. carmichaeli (ETR) on the seed germination and young seedling growth of Lolium perenne, Trifolium repens, and Medicago sativa, the late pasture grasses after cultivation of A. carmichaeli. The results showed that three pasture grasses varied significantly in seed germination and young seedling growth in response to ETR concentrations. Seed germination of M. sativa was stimulated by low ERT concentration (0.01 x g(-1)), while all of pasture grass seeds germinated poorly in solution with 1.00 g x L(-1). Seed soaking with 1.00 g x L(-1) also inhibited significantly the growth of pasture young seedlings, with M. sativa showing the highest seedling height reduction of 42.05% in seeding height, followed by T. repens (40.21%) and L. perenne with about 11%. Cultivation of L. perenne could thus be beneficial to increase whole land productivity in A. carmichaeli-pasture grass cropping systems. In addition, hydrolysis of protein, starch, and inositol phosphates was blocked and free amino acids, soluble sugars and phosphorus were decreased in seeds by seed soaking with ETR, which could be one of the reason for the inhibition of seed germination. There was a significant reduction in root vigor, nitrate reductase, and chlorophyll after the seed treatment with ETR, indicating the suppression of nutrient uptake, nitrate assimilation, and photosynthesis by allelopathic chemicals in ETR, which could lead to the slow growth rate of pasture grass seedlings.
Aconitum ; chemistry ; metabolism ; Allelopathy ; China ; Pheromones ; metabolism ; pharmacology ; Plant Extracts ; metabolism ; pharmacology ; Plant Roots ; chemistry ; metabolism ; Poaceae ; drug effects ; growth & development

AIM: To study the effect of puerarin (Pue) on related cytokines in myocardial hypertrophy induced by isoproterenol (Iso) in rats. METHODS: 40 SD rats were randomly divided into 4 treatments groups: control group, Iso group, Iso+Pue group (Pue group) and Iso+Captoril (Cap) group (Cap group), with 10 rats each group. Ratio of ventricle weight to heart weight, cardiomyocyte diameter, plasma and myocardial tissue NO, ET and Ang Ⅱ levels were measured. RESULTS: Compared with the control group, Iso did induce significant changes in cardiac hypertrophy (P

Aim To investigate the reversal effect of cinobufacine(Cino)on adrimycin(ADM)resistant human breast cancer cell line MCF-7/ADM.Methods The cytotoxic effect of Cino or ADM and the sensitivity of ADM to cells was determined by MTT assay.The intracellular concentration of ADM was detected by HPLC. The expression of P-glycoprotein(P-gp)was examined by flow cytometric(FCM) .Results The maximum non-toxic dose Cino(15 mg?L-1) increased the sensitivity of ADM in MCF-7/ADM,decreased the IC50 of ADM in MCF-7/ADM from 38.14 mg?L-1 to 12.93 mg?L-1, and significantly increased the intracellular concentration of ADM in MCF-7/ADM and reduced the expression of P-glycoprotein.Conclution The results showed that Cino can partially reverse multidrug resistance(MDR)of MCF-7/ADM cells and the mechanism might be associated with the increase of intracellular accumulation of ADM and the reduced expression of P-glycoprotein(P-gp)in MCF-7/ADM cells.

Objective To investigate apoptosis of polymorphonuclear neutrophil and activity of caspase-3 in the peripheral blood and discuss their correlation with development of multiple organ dysfunc- tion syndrome (MODS) after multiple injury. Methods A total of 55 patients with multiple injury were included in the prospective study, and divided into two groups, ie, MODS group (multiple injury patients who developed MODS,) and non-MODS group (multiple injury patients who were free from MODS). The activity of caspase-3 was detected by flow cytometry and the serum levels of IL-6 and IL-10 were detected by ELISA. Then, we evaluated whether the neutrophil apoptesis was correlated with the ser- um levels of IL-6 and IL-10 in patients with MODS. Results Compared with non-MODS group, neu- trophil apoptesis was significantly reduced and activated caspase-3 decreased significantly in MODS group (P <0.05). In MODS group, serum IL-6 was increased significantly while serum IL-10 was decreased significantly compared with non-MODS group. The apoptosis of neutrophil in multiple injury patients with MODS had a negative correlation with IL-6 levels but a positive correlation with IL-10 levels. Conclu- sions The delayed apoptosis of neutrophil due to decreased activated caspase-3 may play partial roles in the development of MODS after multiple injury. IL-6 and IL-10 may contribute to the apoptotic changes.