OBJECTIVE: To explore the relationship between injury age and expressions of erythropoietin (EPO) and its receptor EPOR in the brain tissue of rats after cerebral injury. METHODS: Seventy-two rats were randomly divided into control group (36 rats) and cerebral injury group (36 rats). The rats were sacrificed at 1, 2, 4, 8, 12, 24 h after cerebral injury (6 rats at each time point) and the brain tissues were extracted. The expressions of mRNA and protein of EPO and EPOR at different time points were detected by real-time fluorescent quantitative PCR and Western bloting. RESULTS: The expressions of EPO and EPOR increased within 24 h after injury. The expressions of mRNA and protein of EPO were related to the injury age, and the correlations were 0.875, 0.911, respectively (P < 0.05). The expressions of mRNA and protein of EPOR were related to the injury age, and the correlation coefficients were 0.936, 0.905, respectively (P < 0.05). CONCLUSION The expressions of EPO and EPOR increase gradually in the early stage of the rat's cerebral injury, which are associated with the injury age and could be a useful value for estimating injury age.
Animals ; Brain/metabolism* ; Brain Injuries/pathology* ; Erythropoietin/metabolism* ; RNA, Messenger/metabolism* ; Random Allocation ; Rats ; Receptors, Erythropoietin/metabolism* ; Time Factors

Objective To explore the relationship betw een injury age and expressions of erythropoietin (EPO ) and its receptor EPO R in the brain tissue of rats after cerebral injury. Methods Seventy-tw o rats w ere random ly divided into control group (36 rats) and cerebral injury group (36 rats). The rats w ere sac-rificed at 1, 2, 4, 8, 12, 24 h after cerebral injury (6 rats at each tim e point) and the brain tissues w ere extracted. The expressions of m RNA and protein of EPO and EPO R at different tim e points w ere de-tected by real-tim e fluorescent quantitative PC R and W estern bloting. Results The expressions of EPO and EPO R increased w ithin 24 h after injury. The expressions of m RNA and protein of EPO w ere relat-ed to the injury age, and the correlations w ere 0.875, 0.911, respectively (P<0.05). The expressions of m RNA and protein of EPO R w ere related to the injury age, and the correlation coefficients w ere 0.936, 0.905, respectively (P<0.05). Conclusion The expressions of EPO and EPO R increase gradually in the early stage of the rat’s cerebral injury, w hich are associated w ith the injury age and could be a useful value for estim ating injury age.

Objective:Peripherally inserted central catheter(PICC) has been application in clinical for many years,but the training in the knowledge about maintenance wasn't univesal systematically yet.To analyze the existed problems on the clinical peripherally inserted central catheter in washpipe technology and find out countermeasures. Methods: The self-made questionnaire was used to survey a total of 46 nurses in every department and make on-site operation demonstration. Results: Only 12 nurses' operation was completely correct and the correct rate was 26.1percent,which was related to the training,manager's attention,nurses' participate awareness. Conclusion: The training on nurses' operation of red tube needed to be strengthen.

BACKGROUND:In the past, the surgical treatment of patients with hemophilia B was difficult, the bleeding was difficult to estimate, and the wound healing was difficult. In the perioperative period, the control of coagulation factor IX activity in a safe range can ensure the safety of the operation, resulting in wel wound healing, good recovery, and no significant complications appeared in the long term. OBJECTIVE:To study essentials of perioperative diagnosis and treatment in omarthritis patients with hemophilia B undergoing humeral head replacement, and to analyze the importance on prognosis and rehabilitation. METHODS:The significance of surgical treatment for hemophilia B patients with arthritis, the prevention and treatment of perioperative complications were summarized through literature review. Humeral head replacement was conducted in a patient with hemophilia omarthritis by monitoring coagulation factor activity and infusing human prothrombin complex and frozen plasma. RESULTS AND CONCLUSION:(1) According to the monitoring, patients, whose coagulation factor IX activity increased from 7%to 50%, underwent humeral head replacement. (2) Within three days after replacement, coagulation factor IX activity was control ed>30%, 3 days-2 weeks>20%. No obvious complication was found after surgery. (3) These results suggested that hemophilia B arthritis was commonly treated by surgical treatment, which plays an important role in assessing patient’s condition and treatment. During perioperative period, replacement therapy of human prothrombin complex and control of coagulation factor activity in a appropriate range can effectively prevent postoperative complications.

The classical prescriptions in Typhoid Miscellaneous Disease can be used to alleviate the common hung cancer symptoms and complications like fever, cough, pain and pleural effusion effectively. The preoperative medication can improve lung cancer patients’ surgical tolerance, and postoperative medication can facilitate patients’ postoperative rehabilitation. And if combined with chemotherapy, they cound reduce toxicity and enhance efficiency, enhance the patients’ immunity and improve their life quality. Research shows that classical prescriptions can improve the immunity of lung cancer model mice, facilitate apoptosis of tumor cell and control its migration. This paper sumed up the clinical and experimental research of lung cancer treatment with classical prescriptions in recent 5 years.

Objective:To deeply understand the real experience of family members of patients in the neurosurgery intensive care unit during the period of accompanying them, and to provide reference for managers to formulate nursing countermeasures to improve the coping ability of family members.Methods:Descriptive phenomenological research method in qualitative research was adopted to conduct in-depth interviews with family members of 12 patients admitted to a neurosurgery intensive care unit in Shanghai, manage the interview data with Nvivo11 software, and use Colaizzi's seven-step analysis method to summarize and analyze the topic.Results:There were four themes: adhere to and do not give up, adhere to was due to the trust and affirmation of the medical care, do not give up was because it was difficult to give up blood kinship, adhere to the hope of life because of patients; timing change of emotional experience, first aid period: helpless to rescue and expecting the condition to stabilize, complication peak: understanding the characteristics of the disease and paying attention to the treatment progress, stable period: uncertain prognosis and desire for emotional companionship.The powerlessness of the future; learn to self psychological adjustment.Conclusions:The family members of patients with severe neurological diseases have both negative experience and positive feelings, and the accompanying experience and needs at different stages of the disease are changing dynamically. According to the cognition and needs of the family members, exploring and developing palliative medicine, tapping the potential of the family members from a positive perspective and improving coping ability are one of the ways to maintain the stable mental health of the family members.

Objective To evaluate the clinical role of F-18 fluorodeoxyglucose (FDG)-positron emission tomography (PET) for the diagnosis and staging of prostate cancer.Methods 18F-FDG PET/CT and 99Tcm-MDP ECT whole body bone scan were both performed in 40 patients with prostate cancer proved by biopsy or surgery.We then evaluated the diagnostic value of 18F-FDG PET/CT for prostate cancer.Results Of 40 cases,17 cases were detected by 18 F-FDG PET/CT with a sensitivity of 42.5％.Of the 17 cases with lymphoma metastasis,8 cases were detected by CT while 15 cases were detected by 18F-FDG PET/CT.The sensitivity of 18F-FDG PET/CT is 88.2％.Though 18F-FDG PET/CT and 99Tcm-MDP bone scan have a similar sensitivity in detecting bone metastases,18 F-FDG PET/CT has better specificity and accuracy in detecting bone metastases than that of an 99Tcm-MDP bone scan.Conclusions Though the diagnostic value of 18F-FDG PET/CT in detecting primary focus of prostate is limited,it still has an important role in detecting lymph node metastases and bone metastases.18F-FDG PET/CT has its superiority in prostate cancer staging.It can also help clinicians to select the regimen of treatment.

Objective To establish a HPLC method to determind the content of chlorogenic acid in Jinyin Granules. Method The mobile phase was 0.3%HAc-Methonal(74∶26). The UV detection wavelength was 327 nm. The mobile speed was 0.9 mL/min. Separation column was Kroasil ODS(4.6 mm?250 mm). Result A good linearity was obtained in the range of 65～325 ?g,regression A=24 239 869C-31 292,r=0.999 3. The average recovery was 100.10%,RSD was 0.87%(n=6). Conclusion The method was good for determining chlorogenic acid in Jinyin Granules.

Objective To investigate the roles of transcription factor GATA-3 and T-bet at the fetal- maternal interface in the pathogenesis of unexplained recurrent spontaneous abortion(URSA).Methods The expression of GATA-3 and T-bet mRNA was examined by in situ hybridization.Decidua was obtained from 20 women with URSA and 20 normal pregnant(NP)women.Results(1)The number of GATA-3 positive cells per high power field in women with URSA(25?16)was significantly lower than those in NP women(38?16)(P