BACKGROUND:Mesenchymal stem cels can differentiate into lung parenchymal cels involved in lung injury repair, providing a new approach for the application of mesenchymal stem cels in patients with chronic obstructive pulmonary disease. OBJECTIVE:To observe the effect of bone marrow mesenchymal stem cel transplantation on the repair of airway injury in rats with chronic obstructive pulmonary disease. METHODS:Twenty-four female rats were randomized into four groups: bone marrow mesenchymal stem cel transplantation group (cel transplantation group,n=12); bone marrow mesenchymal stem cels group (cel control group,n=4); model group (n=4); healthy control group (n=4). Rat models of chronic obstructive pulmonary disease were established in the cel transplantation group and model group using fumigation+lipopolysaccharide method; and at 1 day after modeling, model rats were given 1 mL CM-Dil-labeled bone marrow mesenchymal stem cels and 1 mL PBSvia the tail vein in these two groups, respectively. In addition to tracheal injection of normal saline (300 μL) at 1 and 14 days, rats in the cel control and healthy control groups were given 1 mL CM-Dil-labeled bone marrow mesenchymal stem cels and 1 mL PBSvia the tail vein, respectively. At 1, 7, 15 and 30 days after cel transplantation, lung tissue and serum markers of al rats were detected. RESULTS AND CONCLUSION:(1) Hematoxylin-eosin staining showed that emphysema and airway injury was milder in the cel transplantation group than the model group, but severer than the cel control and healthy control groups. (2) The total number of leukocytes and neutrophils in the peripheral blood was higher in the cel transplantation group than the cel control and healthy control groups (P < 0.05); with time, the total number of leukocytes and neutrophils was decreased gradualy. (3) Compared with the cel control and healthy control groups, the interleukin-10 level in the peripheral blood was lower and the levels of tumor necrosis factor-α and granulocyte colony-stimulating factor were higher at 1 day after cel transplantation (P < 0.05). With time, in the cel transplantation group, the interleukin-10 level was increased gradualy, the level of tumor necrosis factor-α was decreased gradualy, and the level of granulocyte colony-stimulating factor was increased first and then decreased, which was highest at 7 days after cel transplantation. (4) Partial CM-Dil-positive cels were positive for CC16. Taken together, bone marrow mesenchymal stem cel transplantationvia the tail vein can improve lung injury of rats with chronic obstructive pulmonary disease, and it is involved in the repair of airway injury through differentiation into epithelial cels and immune regulation.

Objective To explore the operation al skills of laparoscopic appendectomy(LA) and to improve its proficiency.Methods The clinical data of 121 patients under went LA during February 2013 to May 2014 were summarized retrospectively.Results All the 121 cases were completed successfully with no complication.Conclusion With the development of laparoscope and accumulation of experiences LA can be safe and complications can be minimized if the this operation skill being obtained.

ObjectiveTo investigate the mechanism of TNF-? on regulating the blood-labyrinth barrier permeability of cochlea of guinea pig.MethodsThe stria vascularis was acquired from guinea pigs and the endothelial cells were cultured of with purified to establish the models of the blood-labyrinth barrier according to our previous researches.The models were treated with TNF-?(0.05,0.1,0.2 ng/ml)for 30,60,90 min or with serum-free DMEM as control.Then their permeability was measured by Evens blue technique.ResultsTNF-? increases Evans blue transfer across endothelial cell monolayer(P

Objective To observe the changes of F-actin of strial capillary endothelial cells from guinea pig cochlea after TNF-? treatment so as to further study the mechanism of permeability of those cells.Methods Strial capillary endothelial cells were dissociated from guinea pig cochlea and cultured respectively with 0.05,0.1,0.2 ng/ml TNF-? for 90 min,then detected by immunofluorescence laser confocal microscopy for F-actin concentrations.The blank control was set simultaneously.Results TNF-? decreased the content of F-actin in strial capillary endothelial cells(P

Objective:To observe the effect of complex reinforcing [Shao Shan Huo (Mountain-burning Fire)] and reducing [Tou Tian Liang (Heaven-penetrating Cooling)] manipulations on motion and balance of the lower limbs in patients with subacute combined degeneration of the spinal cord (SCD). Methods:A total of 100 SCD cases were randomly allocated into an observation group and a control group by their visit sequence, 50 cases in each group. On the basis of Western medical treatment, cases in the observation group were also treated with acupuncture therapy plus complex reinforcing-reducing manipulation; whereas cases in the control group were only treated with the same Western medical treatment as those in the observation group. Before and 2 months after treatment, kinematic parameters including muscle force, muscle tone and range of motion of the hip and knee joints were measured. In addition, balancing parameters including the path length of center of pressure (COP), peripheral area, COP path length per unit area and rectangular area were also measured before and after treatment. Results:After treatment, there were statistical intra-group differences in COP path length, peripheral area, COP path length per unit area, left-right offset, rectangular area, coefficient of stability and coefficient of weight distribution (P<0.01). There were statistical inter-group differences in muscle force, muscle tone and range of motion of hip and knee joints (P<0.01). The total effective rate was 83.3% in the observation group, versus 60.0% in the control group, showing a statistical difference (P<0.05). Conclusion:Complex reinforcing-reducing manipulations can improve the lower limb motion and balance in SCD patients.

Objective To assess the effects of Mtwo for root canal therapy in the aged patients .Methods One hundred and fifty-two root canals in 64 teeth for root canal therapy (RCT) in aged patients(60~70years)were randomly divided into M groups and K groups.Mtwo machine with nickel titanium file was used in M group (32 teeth, 78 root canals), and stainless steel file in K group (32 teeth, 74 root canals).Root canal preparation time, root spread reaction, and canal filling effects were compared between two groups.Results The root canal preparation and filling in group M were better than in K group ( P <0.05).The time consumption of RCT in M group was shorter than in group K .Conclusions Mtwo shows good shaping ability and is safe for aged patients in root canal treatment with high efficiency .

ObjectiveTo observe the effect of pelvic exercise to improve walking ability in hemiplegia. Methods60 stroke patients were devided into 2 groups, pelvic exercise group( 30 cases) and control group(30 cases). The walking ability in all patients was evaluated to compare the effect between two groups.ResultsThe excellent and good rate was 93.3% in pelvic exercise group. As compared with control group, there is a significant improvement in walking ability in pelvic excise group. ConclusionsPelvic exercise can significantly improve walking ability in stroke patients.

Air Pollutants ; analysis ; Dust ; analysis ; Environment Design ; Environmental Monitoring ; instrumentation

Objective To investigate the effectiveness and feasibility of laparoscopic splenic artery ligation in the treatment of hypersplenism and thrombocytopenia in children. Methods From August 2014 to December 2014, four children with hypersplenism and three children with ITP (idiopathic thrombocytopenic purpura) were treated in our hospital.Laparoscopic splenic artery ligation was performed in all the patients.During the operation, the gastric colon ligament was cut with an ultrasonic knife, and the gastric wall was suspended to expose the pancreas and the spleen.The splenic artery was ligated at the upper edge of pancreas closing to the splenic hilum.The splenic venous branches were dissected and ligated at the splenic hilum by using the Hem-o-lok, which leaded to an area of splenic infarction more than 50%. Results The laparoscopic splenic artery ligation was successfully performed in all the patients, without conversion to open surgery.The operative time was 120-150 min ( mean, 126 min) , and the intraoperative blood loss was 10-20 ml ( mean, 15 ml) .No patient underwent blood transfusion.The length of postoperative hospital stay varied from 4 to 11 days (mean, 6.6 days).The splenic length significantly decreased postoperatively [(13.6 ±2.6) cm vs.(15.1 ±1.7) cm, t=3.199, P=0.049], and so was the splenic thickness [(3.8 ±1.0) cm vs.(4.1 ±0.8) cm, t =3.703, P=0.034].Partial splenic infarction and decreased splenic blood flow were found in the 7 patients by postoperative ultrasound.After surgery, four children suffered from fever, which were 38.8 ℃, 39.0 ℃, 38.6 ℃, and 39.2 ℃, with the duration of fever of 2, 4, 8, and 5 days, respectively.All the patients were followed-up for 6 -10 months (mean, 8.4 months).The complete blood cell count was within normal range. Conclusion The laparoscopic splenic artery ligation is an effective treatment for hypersplenism and thrombocytopenia in children.