1.An animal model of lung transplantation:damage, protection and immune response
Chinese Journal of Tissue Engineering Research 2014;(5):797-802
BACKGROUND:Establishing an animal model of lung transplantation has operational significance to the development of clinical lung transplantation.
OBJECTIVE:To analyze and summarize animal selection, model establishment method, points to note and mechanism of lung ischemia-reperfusion injury and pulmonary immune rejection in the establishment of animal models as preclinical research of lung transplantation.
METHODS:A computer-based retrieval of CNKI and PubMed database from January 1982 to September 2013 was performed for literature related to lung transplantation. The key words were“lung transplantation, animal models, reperfusion injury”in English and Chinese, respectively. After eliminating duplicate and obsolete literature,50 articles were included for further analysis.
RESULTS AND CONCLUSION:Currently, animal experiments about al ogeneic lung transplantation are
common, which are of significance for clinical lung transplantation. Single lung transplantation is mainly seen in animal models. The commonly used lung transplantation models include single lung orthotopic murine model, rabbit orthotopic lung transplantation, canine and porcine orthotopic left lung transplantation, canine bilateral sequential lung pulmonary transplantation, autologous pig lung transplant model, rabbit lung ischemia-reperfusion model. Because the anatomical and physiological features are similar to humans, pigs are becoming the preferred choice for large animal experiments. Animal experiments and clinical studies have shown that lung ischemia-reperfusion injury in lung transplantation represents a biphasic pattern:early occurrence of lung ischemia-reperfusion injury (24 hours after infusion) is related to lung donors, and the late occurrence mainly depends on receptors. Its pathophysiology runs through the whole process of donor lung resection, preservation and reperfusion as wel as postoperative management. Immune rejection is a complex immune response induced by identifying the donor cellsurface histocompatibility antigen, and activated T lymphocytes play a crucial role in the immune response in organ transplantation.
2.Cloning and Screening of Mutant Human Hemoglobin Gene(?_(99) ,?_(82) Lys→Cys)
Hao ZHANG ; Quanli WANG ; Qi FENG
Chinese Journal of Blood Transfusion 1988;0(04):-
Objective:To mutate the codons which code the lysine at loci ? 99 ,? 82 in human hemoglobin gene to the codons which code the cystein.Methods:The objective gene to be mutated was cloned into pAlter Ex2 plasmis and then was mutated with the site directed mutagenesis mediated by oligonucleotide.Results:The sequencing of clones obtained by preliminary screening showed that the anticipated results were reached without any unexpected mutation.Conclusion:The results lay a foundation for the further development of research on expression and purification of recombinated mutant human hemoglobin gene.
3.Serial Expression of Human ?,? globin Genes in Escherichia coli
Hao ZHANG ; Quanli WANG ; Qi FENG
Chinese Journal of Blood Transfusion 1988;0(02):-
Objective To make an attempt to express serialy the human ?,? globin in Escherichia Coli in order to carry out the research on blood substitute based on the genetic recombination of hemoglobin.Methods The routine molecular biology technology was used.Results The serial genetic fragment of ?,? globins containing the hemoglobin was amplified by PCR.The genetic sequencing showed that there was no unexpected mutation.And then,the serial genes were cloned into the pBV220 expression carrier.Undergoing thermal inducement,the expression product could reach about 20% of total bacterial protein.The expression product assumed the form of inclusion body,and it was vertified by Western Blotting.Conclusion This much can lay the solid foundations for further launching the research on expression and pruification of recombinant human hemoglobin genes.
4.Studies on structure, physicochemical properties and anticancer activity of glycyrrhizic acid aniline-platinum complex compounds
Jing ZHANG ; Runguang SUN ; Hao QI ;
Chinese Traditional and Herbal Drugs 1994;0(11):-
Object To investigate the structure, physicochemical properties and anticancer activity of three kind of glycyrrhizic acid aniline platinum complex compounds. Methods Using the FT IR methods, elementary analysis and X ray diffraction, the physicochemical properties of the tested drugs were determined; by means of MTT test the inhibitory effects of the drugs on human tumor cell growth were examined, with hematopoietic cell K 562 , melanoma cell LiBr and liver cancer cell 7721 as the model of anticancer activity. Results Experimental values of elemental analysis of three complex compounds tally with the theoretically calculative values. The infrared spectroscopy showed that the functional group location agreed with the structure. The X ray diffraction exhibited that this type of the compounds was monoclinic system. Conclusion The experimental results show that the anticancer activity of the test complex compounds is related to their concentration and ligands. Magnetic field treatment has significant influence on anticancer activity of this type of compounds.
5.Conditional logistic model multivariate analysis on risk factors of nasopharyngeal carcinoma in Hunan.
Hao WANG ; Shuo-qi LI ; Lei ZHANG
Journal of Central South University(Medical Sciences) 2005;30(3):356-359
Adult
;
Aged
;
China
;
epidemiology
;
Female
;
Humans
;
Logistic Models
;
Male
;
Middle Aged
;
Multivariate Analysis
;
Nasopharyngeal Neoplasms
;
epidemiology
;
etiology
;
Risk Factors
6.A prospective randomized study of TOT and TVT procedure for female intrinsic sphincter deficiency(ISD)
Weiyu ZHANG ; Qi WANG ; Xiaopeng ZHANG ; Hao HU ; Kexin XU
Chinese Journal of Urology 2016;37(10):777-780
Objective To compare the outcomes of TOT and TVT procedure treating female intrinsic sphincter deficiency (ISD).Methods From May 2010 to September 2015,42 stress urinary incontinence (SUI) patients whose abdominal leak point pressure was less than 60 cmH2O were enrolled in this study.Thirty-five patients were followed up.The mean age was (56.8 ±10.5) years,with a range of 30-80 years.The mean history was(10.5 ± 9.1)years,with a range of 4 months to 30 years,with 26 (74.3%) of them being postmenopausal,5 (14.3%) having a history of pelvic surgery,and no pelvic organ prolapsed or hormone replacement.All of them were randomly divided into 2 groups to undergo either TVT operation (13 cases) or TOT operation (22 cases).The baseline characteristics of the two groups including age,length of history,urodynamic parameters and scale scores showed no significant difference.The scales including urinary incontinence severity score (UISS),detrusor instability score (DIS),Quality of Life Scale Evaluation (I-QOL),lower urinary tract symptoms affect score (UDI-6) were used.The outcomes between TVT group and TOT group were compared.Result After procedure,patients in TVT group got a lower UISS score than TOT group(17.2 ± 2.2 vs.17.7 ± 3.1),and their severity of urinary incontinence improved significantly (P < 0.05).Patients from TVT group got a lower DIS score than TOT group (12.6 ± 4.2 vs.14.2 ± 3.5),and their detrusor instability symptoms improved more significantly (P < 0.05).Patients from TVT group got a higher I-QOL score than TOT group(17.5 ± 14.5 vs.16.1 ± 13.0),and their quality of life improved more significantly (P < 0.05).Patients from TVT group got a lower UDI-6 score than TOT group (10.1 ± 3.0 vs.11.2 ± 3.4),and their lower urinary tract symptoms improved more significantly (P < 0.05).Conclusion Urinary incontinence of female ISD patients were improved greater by TVT than TOT procedure.
7.CT value characteristics and significance of Graves disease and Hashimoto's thyroiditis
Xianghui KANG ; Lijuan QI ; Shidan HAO ; Ying XU ; Jingyi ZHANG
Clinical Medicine of China 2014;30(1):90-92
Objective To investigate the change and clinical significance of thyroid CT value in patients with Graves disease and Hashimoto' s thyroiditis (HT).Methods One hundred and twelve Graves patients (GD group),54 HT patients (HT group) and 36 healthy people (NC group) were selected as our subjects.Computed tomography (CT) value of thyroid were measured.Results The CT value of the left thyroid gland was (70.53 ± 15.44) Hu in GD group,while (70.50 ± 16.01) Hu at the right side.In HT group,the CT value of the left thyroid gland was (53.77 ± 4.88) Hu,and (52.38 ± 6.67) Hu at the right side.The CT value of the left and right sides was (99.66 ±9.80) Hu and (100.77 ± 10.66) Hu in the NC group respectively.The CT value of GD and HT group were lower than that of the NC group (P < 0.01),and the CT value of the HT group lower than that of the GD group more significant (P < 0.01).Conclusion (1) The CT value of the GD group and the HT group are significantly lower than the NC group and the CT value of the HT group reduces more apparently than that of the GD group.(2) CT values can be used as an early,non-invasive diagnosis of GD and HT secondary indexes.
8.Comparison of placement of peripherally inserted central catheters using vascular ultrasound guidance system and traditional method in 938 tumor patients
Yongzhi QI ; Yan GUO ; Xiaoxia XU ; Hao ZHANG ; Li LI
Chinese Journal of Clinical Nutrition 2012;20(4):253-255
Objective To compare the placement of peripherally inserted central catheters(PICC)by using vascular ultrasound guidance system and traditional method.Methods Totally 938 patients undergoing PICC were divided into the traditional method group and the vascular ultrasound system and microintroducer techniques group according to the puncture time.The differences in the one-attempt success rate and the overall success rate of the placement and the incidences of complications were compared between the two groups.Results The one-attempt success rate was 93.01% in the traditional method group and 98.76% in the vascular ultrasound system and microintroducer techniques group(P =0.005).The successful rate of PICC placement was 100%.The incidence of complication was 11.29% in the traditional method group and 2.47% in the vascular ultrasound system and microintroducer technique group(P =0.000).Conclusion The PICC placement using vascular ultrasound guidance system and microintroducer techniques can increase one-attempt success rate and decrease complications.
9.Harmine has no lung-protective effects in a canine model of lung ischemia-reperfusion injury
Hao ZHANG ; Hai QI ; Yuanming LI ; Jiahua CHEN
Chinese Journal of Tissue Engineering Research 2014;(36):5805-5812
BACKGROUND:Inflammatory cellactivation and the generation of oxygen free radicals are important factors of lung ischemia-reperfusion injury. Adding the drugs with anti-inflammation and antioxidant effects into the lung preservation solution used, can improve the protection fluid, and play a crucial role in the study of lung ischemia-reperfusion injury and protection of the function of transplanted lung. OBJECTIVE:To discussion the effect of harmine in canine model of pulmonary ischemia-reperfusion injury. METHODS:Twelve healthy hybrid dogs were randomly divided into two groups, with six rats in each group. A canine model of lung ischemia-reperfusion injury was established, and the protecting liquid was perfused with the clockwise irrigation method. Control group:low potassium dextran protective fluid;experimental group:low potassium dextran+harmine protective fluid. After 2 hours of ischemia, the left lung circulation was recovered. The left lung tissue and blood samples were col ected from two groups of animal models after reperfusion, and their cytokines levels and the lung wet/dry weight ratio were detected and calculated. Bronchoalveolar lavage fluid was col ected to observe the pathological indicators. The main pulmonary artery pressure, left and right pulmonary artery pressure were recorded by continuous monitoring. RESULTS AND CONCLUSION:There were no statistical y significant differences in the interleukin-17, tumor necrosis factorαand endothelin 1 content in the left lung tissue and the blood between the two groups at 2 and 4 hours after reperfusion (P>0.05). After 4 hours of reperfusion in both groups, the neutrophil number, the number of lymphocytes, alveolar edema index, and vascular wal damage in bronchoalveolar lavage fluid showed no statistical y significant differences (P>0.05). Through the analysis of variance, the main pulmonary artery pressure, left pulmonary artery pressure and right pulmonary artery pressure also had no statistical significance between the two groups (P>0.05). By the analysis of cytokines, pathological indicators, lung wet/dry weight ratio, and pulmonary arterial pressure, harmine has no significant lung protection effect in canine model of lung ischemia-reperfusion injury.
10.Histocompatibility of polyaiticglycolic acid/RNAⅢ inhibiting peptide sustained release microspheres
Xiaobin ZHANG ; Libo HAO ; Jifang WANG ; Qi YAO ; Maohua LIANG
Chinese Journal of Tissue Engineering Research 2007;0(01):-
AIM: RNAⅢ inhibiting peptide (RIP) has been previously proved to possess good histocompatibility and safety for preventing and curing staphylococcal infection, and this study evaluated histocompatibility of polyaiticglycolic acid/RIP (PLGA/RIP) sustained release microsphere. METHODS: The experiment was performed in the Orthopedic Institute, Pharmacologic Research Institute and Animal Experimental Center of General Hospital of Chinese PLA from October 2005 to October 2007.①Preparation of PLGA/RIP: The solid-phase synthesis (Fmoc) method was used to synthesize RIP from C end to N end, then the synthesized peptide was purified by the reverse phase high performance liquid chromatography, and composition was collected by means of ultraviolet absorption peak. The purified RIP was obtained after freezing and drying. Liquid-phase multiple emulsion method was used to synthesize PLGA/RIP microsphere of 50-70 ?m diameter.②Acute general toxicity test was studied in PLGA/RIP. Effect of PLGA/RIP on the cell proliferation was detected with cytotoxicity test by MTT method. Intramuscular implanting test was used to observe the irritation reaction of muscles by implantation materials. Sensitivity test was used to observe the sensitization of PLGA/RIP. Changes of animal's body temperature were determined with pyrogen test. RESULTS: ①Acute general toxicity test: Neither toxicosis reaction nor animal death was found after animals were injected with 100% and 50% eluents of PLGA/RIP peritoneally. Animal's body weight was not changed significantly.②Cytotoxiciity test by MTT method: The average proliferation rate of cell in two kinds of eluents exceeded 85% and cytotoxicity was graded in 1 rank, indicating no cytotoxicity.③Intramuscular implanting test: At 4 weeks after RIP and PLGA/RIP were implanted into the animals, there was not obvious synathresis, denaturation or necrosis in tissues. No inflammatory cell infiltration occurred around the materials. There had been the fibrous capsules around the materials.④Sensitivity test: Average primary irritation index of three groups were 0.38, 0.33 and 0.31 respectively. There was no significant difference among three groups.⑤Pyrogen test: Fervescence of each animal in the experiment was under 0.5 ℃, confirming that the materials had no pyrogenic characteristics. This was in coincidence with evaluation criterion of pyrogen test. CONCLUSION: PLGA/RIP has good histocompatibility and safety, without general toxic reaction, cytotoxicity, immunological rejection, hypersensitive response or pyrogenic characteristics.