Objective To investigate the prevalence of low T3 syndrome in patients with acute myocardial infarction(AMI) and explore the effect of low T3 syndrome on outcome of AMI.MethodsThree hundred and thirty-eight patients with AMI admitted to cardiac care unit(CCU) underwent examinations of thyroid function and cardial ultrasound,and were further categorized according to thyroid hormone profile.The records of noninvasive bi-level positive airway pressure(BiPAP)ventilation utilization,length of hospital stay,mortality during hospitalization were evaluated,and the related factors were analysed.ResultsOne hundred and thirty-nine of the 338 patients(41.12%) with AMI complicated with low T3 syndrome.Free triiodothyronine(FT3) was the independent influential factor for length of hospital stay.Low FT3 was significantly correlated with noninvasive BiPAP ventilation utilization and mortality during hospitalization.The average time of follow-up was(21.4?8.1) months.It was revealed by multivariate Cox regression analysis that FT3 was the chief predictor for cumulative death(risk ratio,4.25;95% confidential interval,2.30-7.87),followed by age and left ventricular ejection fraction.ConclusionThe recognition of AMI complicated with low T3 syndrome plays an important role in predicting the disease severity and outcome.

Objective To evaluate the clinical significance of bone marrow minimal residual disease (MRD) monitoring by multi-parameter flow cytometry (FCM) regularly after the first complete remission (CR1) in patients with acute myeloid leukemia (AML).Methods A total of 63 paitents with AML who had got CR1 after chemotherapy were regularly monitored for MRD in bone marrow by FCM,and MRD ≥ 10-4 was positive.According to the latest standards of National Comprehensive Cancer Network (NCCN) for disease risks,they were categorized into three groups:better risk group (20 cases),intermediate risk group (27 cases) and poor risk group (16 cases).The probability of continuous complete remission (CCR) was calculated by KaplanMeier formula,and the statistical difference between MRD positivc and MRD negative CCR probabilities was evaluated by log-rank test.Results The positive rates of MRD were 20％(4/20),30％(8/27) and 10/16 in better risk group,intermediate risk group and poor risk group respectively.The difference between better risk group and intermediate risk group had no statistical significance (P=0.454),and the difference between poor risk group and intermediate risk group had statistical significance (P =0.035).Twenty-two cases showed positive MRD,and 41 cases showed negative MRD.The probability of CCR at 24 and 36 months in MRD positive patients were 18％ (4/22),18％ (4/22),in MRD negative patients were 83％ (34/41),80％ (33/41),and there were significant differences (P ＜ 0.01).Conclusions The dynamic detection of MRD by FCM can be used to evaluate the therapeutic effect and prognosis of AML.MRD monitoring has important clinical significance and can help to adjust the intensity of chemotherapy,carry out individualized treatment,predict prognosis,and choose appropriate therapy.

Objective To explore the significance of palpation-negative breast tumor resection with ultrasound-guided methylene blue labeling and percutaneous suture traction.Methods 46 cases of small breast tumors with the diameter from 0.5 cm to 1.0 cm were double labeled with methylene blue under the guide of ultrasound l hour preoperatively.The tumors were fixed,drew outward with percutaneous suture and resected accurately.All the 46 cases were rechecked by ultrasound to verify whether residual or false resection occurred 1 month later.Results All the 58 tumors of the 46 patients were accurately resected.No residue or false resection occurred.The average operation duration was 10 min to 20 min.Conclusion Palpable-negative breast tumor resection with ultrasound-guided methylene blue labeling and percutaneous suture traction has the advantages of small invasion,accurate positioning and short operation duration.

To construct retroviral vector carrying human interleukin-3 c0mplementary DNA(HuIL-3 cDNA) under control of human a-fetoprotein gene enhancer core sequence and human SV4O pro-moter. Methods and Results: HuIL-3 cDNA was inserted into polylinker site of retroviral vector pMNSMto construct retr0viral vector pMNS-IL-3, in which the transcription of HuIL-3 cDNA was drived by SV40early region promoter. Human Q-fetoprotein gene enhancer core sequence was released from plasmidpGEM. 7Zf-AFPe and inserted into the polylinker site of pMNSM. Then human interleukin-3 cDNA wasinserted int0 p0lylinker site to construct retroviral vector pMNSA-IL-3, in which HuIL-3 cDNA transcrip-tion was drived by SV40 early region promoter and enhanced by human a-fetoprotein enhancer core se-quence- Conclusion: The vectors are of significance for hepatoma-specific gene therapy.

OBJECTIVETo detect the expression of fibroblast activation protein(FAP) in colorectal cancer tissue, and to investigate the association between expression of FAP with pathological parameters.

METHODSFifty-five cancer tissues and 50 normal colorectal samples were examined using immunohistochemistry with anti-FAP polyclonal antibody. The distribution of positive cells in different tissues, and associations of positive cell number with tumor staging, lymph node metastasis and tumor invasion were investigated to evaluate the effects of FAP on pathological progress in colorectal cancer.

RESULTSNo FAP expression was observed in 50 normal colorectal tissue samples. FAP positive cells were seen in carcinoma associated fibroblasts(CAFs), and in few colorectal cancer cells. The numbers of FAP positive cells in tissue samples of TNM III(-IIII((40.1±15.9) was significantly greater than that of TNMI(-II( (18.3±7.7)(P<0.01). Furthermore, the number of FAP positive cells in tissue samples with lymph node metastasis (44.4±13.3) was also significantly higher than those without lymph node metastasis (18.5±8.1)(P<0.01). Significant positive correlations were found between the number of FAP-positive cells with the tumor TNM staging and lymph node metastasis(r=0.544 and r=0.793, respectively)(P<0.01). The number of FAP-positive cells was 25.2±8.9 in T2, 32.41±19.30 in T3, and 29.2±16.5 in T4. The association between number of positive cells and depth of invasion was not statistically significant(P>0.05).

CONCLUSIONSThe FAP mainly expresses in CAFs locating in colorectal cancer tissues. The number of FAP positive cells is positively correlated with TNM staging of colorectal cancer and lymph node metastasis.

Adult ; Aged ; Aged, 80 and over ; Case-Control Studies ; Colorectal Neoplasms ; metabolism ; pathology ; Female ; Gelatinases ; metabolism ; Humans ; Lymphatic Metastasis ; Male ; Membrane Proteins ; metabolism ; Middle Aged ; Serine Endopeptidases ; metabolism

Objective We invented mammary lump skin-positioning membrane (named scare membrane) to facilitate localization of mammary lump during ultrasound scan.This study is to measure the radius of mammary of Chinese adult women for designing different types of skin-positioning membrane.MethodsThe radius of mammary glands in 236 cases of adult females was detected with color Doppler ultrasound at 5 different clock positions:12:00,3:00,6:00,9:00 and 10:30 or 1:30.SPSS 16.0 statistical software was used to analyze the data.The cut-off values of mammary glands radius at 99％,95％,75％ and 50％ were calculated.Paired t tests or nonparametric tests (relative sample rank sum tests) were used to verify the consistency of mammary gland radius between left and right sides.ResultsThe cut-off value of 95％ mammary gland radius was:7.700cm at 12:00,7.810 cm at 13:00,8.100 cm at 3:00,5.330 cm at 6:00 and 6.300 cm at 9:00 for left mammary gland ; 7.500 cm at 12:00,6.015 cm at 3:00,5.500 cm at 6:00,8.510 cm at 9:00 and 7.930 cm at 10:30 for right mammary gland.In comparison of left and right mammary gland radius,the difference had statistical significance between the group of left side at 1:30 and right side at 10:30,the group of left side at 3:00 and right side at 9:00(P ＜0.05).The right mamma was relatively larger.The cut-off values of the right mamma at the above two clock points were taken as radius of scale membranes while the average of percentage cut-off values at 12:00,3:00 of both mammas,left side at 9:00 and right side at 3:00 are taken as radius of scale membranes.ConclusionsAccording to the cut-off values of 99％,95％,75％ and 50％ radius of adult female mammary glands,mammary lump skin-positioning membrane radius can be classified into 4 size-types:extra large,large,medium and small.The precise classification of radius of mammary scale membranes according to mammary glands of adult females provides convenience for production,manufacture and clinical application of mammary lump skin-positioning membrane.

Objective To investigate the situations of close contacting with MDR-TB in family and analysis the necessity and urgency of family protection.Methods Nine typical primary MDR-TB cases in our hospital that close contacted with family pulmonary TB patients.their relatives contacted with them and their treatment prognosis were retrospectively analyzed.Results All of the 9 cases were transmitted by lineal MDR-TB relatives.In one case,3 generations were pumonary TB patients.All of 4 family members were pulmonary TBpatients in another case.3 cases were treated with operations:2 cases with pulmonary lobectomy and 1 case with intestinal resection.Treatment prognosis:3 cases cured,2 cases not cured,4 cases dead.Conclusions Family close contacting with MDR-TB patients should be intervened earlier:including health education,effective protection and health examination regularly et al.

Objective To investigate the supply of iodized salt in non-excessive iodine counties and iodine-free salt in excessive iodine counties at household level in Hebei province so as to provide a basis for the prevention and control of iodine deficiency disorders(IDD). Methods According to the national project of IDD surveillance,the county was taken as the elementary sampling unit. The towns and villages were selected by systematic and random sampling in every county and households were chosen by random sampling to collect their edible salt in Hebei province in 2008. The salt iodine content in non-and excessive iodine regions was detected by direct titrition method and semiquantitative method respectively. Results all 48 448 salt samples were collected from 167 non-excessive iodine counties. Weighed by the population of counties,the rate of non-iodized salt was 4.73%. Iodized salt accounted for 95.27%,out of which,96.13% were qualified and the consuming rate of qualified iodized salt was 91.96%. Eighty point eighty three percent(135/167) of the counties covered by iodized salt above 95%,92.81% (155/167) passing rate of iodized salt above 90% and 82.04 (137/167) consuming rate of qualified iodized salt. All 1466 salt samples were collected in 5 counties with excessive water iodine content and the coverage rate of iodine-free salt was 93.25%(1367/1466). Conclusions In a nutshell,the national targets for preliminary elimination of IDD have been achieved in regions of non-excessive iodine of Hebei province. Nevertheless,the coverage rate of iodized salt and qualified iodize salt rate in some counties are still below the national standard. Therefore the prevention and control of IDD need to be strengthened. The supply of iodized salt in excessive iodine regions should be timely stopped.

OBJECTIVETo analyze the status of anemia among children under 3 years old from western China based on the data gathered from the project area.

METHODS9616 children under 3 years old and their families were investigated, using stratified random sampling method in 46 counties in western China. To measure the content of haemoglobin by cyanmethemoglobin and hemocue, under the diagnostic criterion as 110 g/L for child aged 6 months to 6 years old, proposed by WHO and UNICEF.

RESULTSThe overall prevalence of anemia among child under 3 years old in ten provinces was 33.8%. Prevalence of anemia varied along with age differences. The peak of anemia prevalence was 12-18 months. The related risk of anemia between mother and child was 3.271. Data from logistic regression showed that mother being anemic, cooking with iron pan, cooking for child separate from adults and gender had statistical significance to their children's anemia.

CONCLUSIONThe feasible measures should be developed to reduce the prevalence of anemia.

Age Distribution ; Anemia ; epidemiology ; metabolism ; Child ; Child, Preschool ; China ; epidemiology ; Female ; Hemoglobins ; analysis ; Humans ; Infant ; Infant, Newborn ; Male

Objective To study the effect of nobiletin on the invasion and migration capabilities of breast cancer MDA-MB-231 cells.Methods One blank group and 5 concentrations of (nobiletin 12.5,25,50,100,200 μmol · L-1) experimental groups were set up.The 3 -(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide was used to observe the effects of different concentrations of nobiletin on proliferation of human breast cancer cell line MDA-MB-231.Transwell chamber and wound healing assay were used to detect the effects of nobiletin on the migration and invasion of MDA-MB-231.The cyclooxygenase 2 (COX-2),matrix metalloproteinase-2 (MMP-2),matrix metalloproteinases-9 (MMP-9),and mitogen activated protein kinase (MAPK) signal pathway related protein expressions were analyzed with Western blot.Results The proliferation of human breast cancer cell line MDA-MB-231 was inhibited by nobiletin in a dose-and time-dependent manner.The OD570 nm of two different concentrations (30,50 mol · L-1) were 0.32 ± 0.02 and 0.27 ± 0.01,compared with the blank group 0.62 ± 0.07,the difference was statistically significant (P ＜ O.05).After working 24 h with nobiletin,migration distances of three different concentrations (10,30,50 mol · L-1) were (75.52 ± 0.69) μm,(111.73 ± 23.12) μm,(115.71 ± 28.09) μm,it obviously increased compared with the blank group (47.03 ± 8.29) μm,the difference was statistically significant (P ＜ 0.05).The expression of MMP-2 in three different concentrations of nobiletin were (14.17 ± 5.26) × 103,(9.33 ± 1.76) × 103,(9.28 ± 1.76) × 103 respectively,compared with the blank group (22.51 ±0.90) × 103,the differences in the last two groups were statistically significant (both P ＜0.001).Similarly,expression of MMP-9 were (16.82 ±2.38) ×103,(9.91 ±2.08) ×103,(8.86 ±3.26) × 103 respectively,compared with the blank group (21.94 ± 1.50) × 103,the differences were statistically significant (P ＜ 0.05,P ＜ 0.01,P ＜ 0.01).The expression of JNK in three concentrations of nobiletin were (11.78 ± 3.59) × 103,(8.65 ± 1.89) × 103,(6.21 ± 0.60) × 103 respectively,compared with the blank group (13.26 ±2.69) × 103,the difference of high concentration was statistically significant (P ＜ 0.05).Similarly,the expression of p38MAPK were (11.05 ± 0.42) × 103,(10.08 ± 0.83) × 103,(6.94 ± 0.14) × 103 respectively,compared with the blank group (12.38 ± 0.14) × 103,the differences of three concentrations were statistically significant (P ＜0.01,P ＜ 0.01,P ＜ 0.001).Conclusion Nobiletin has a certain role in inhibiting the invasion and migration of MDA-MB-231 cells in vitro,and its mechanism may be related to the down-regulation of MAPK signaling pathway and inhibiting the expression of MMP-2,MMP-9 and so on.