Objective To study the method based on gradient vector flow (GVF) and particle swarm optimization (PSO) for realizing multimodal medical image registration and improving its accuracy. Methods In view of three major components of image registration, i.e. the feature space, the similarity metric and the search strategy, a novel method was proposed with three improvements. Firstly, the GVF field was employed as the feature space. Then three similarity metrics were proposed based on GVF field. Finally, an improved PSO combined with crossover mechanism of genetic algorithm was utilized to search for the optimal transformation of two images. Results With 54 times of experiments on both simulated and real medical images, it was demonstrated that this method accurately registered the multimodal medical images to be superior to the method based on PSO of pixels, and the Walsh transform method. Conclusion The method based on GVF and PSO is effective for multimodal medical image registration.

OBJECTIVETo investigate the action mechanisms of the FZD5 gene in prostate cancer bone metastasis and search for some new treatments for this disease.

METHODSWe determined the expression level of the FZD5 gene in prostate cancer PC3 cells and, after transfection of siRNA into the PC3 cells and silence of the FZD5 gene, observed the changes in the migration and proliferation of the cells. We established the model of prostate cancer bone metastasis by tibial injection of prostate cancer cells in the nude mice. Then we injected control siRNA and FZD5-silenced siRNA into the tibia of the mice followed by evaluation of tumor-induced bone destruction by X-ray imaging at 0, 1, and 3 weeks and by HE staining at 3 weeks after injection.

RESULTSAfter transfection of FZD5-silenced siRNA into the prostate cancer PC3 cells, the expression of the FZD5 gene was decreased about 70%. The rate of cell proliferation was significantly lower in the gene silencing group than in the control (P < 0.05), and that of cell migration dropped by 30% in the former as compared with the latter group at 48 hours after FZD5 silencing (P < 0.05). At 3 weeks after injection of control siRNA or FZD5-silenced siRNA into the tibia of the mice, osteolytic damage was observed in both groups, though less in the FZD5 silencing group, with only a few remaining bone trabeculae visible.

CONCLUSIONSilencing the FZD5 gene can reduce the migration and proliferation of prostate cancer cells, help to suppress bone metastasis and destruction, and thereby improve the survival rate and quality of life of the patients.

Animals ; Bone Neoplasms ; genetics ; prevention & control ; secondary ; Cell Line, Tumor ; Cell Movement ; genetics ; Cell Proliferation ; genetics ; Frizzled Receptors ; genetics ; physiology ; Gene Expression ; Gene Silencing ; Male ; Mice ; Mice, Nude ; Osteolysis ; Prostatic Neoplasms ; genetics ; metabolism ; pathology ; Quality of Life ; RNA, Small Interfering ; administration & dosage ; genetics ; Transfection

Objective To evaluate the long term effects of laparoscopic surgery on duodenal ulcer with acute perforation. Methods From 1995, Twenty-five patients with duodenal ulcer perforation and diffused peritonitis were diagnosed definitely by TV laparoscopic technique, and the perforations were sutured and repaired by greater omentum under laparoscopy. Six cases of them were performed parietal cell vagotomy simultaneously. After operation the drain was put into abdominal cavity and H2 receptor blocking agent, proton pump inhibitor or plus antihelicabater pylori therapy were used. Results All operations of 25 cases were accomplished under laparoscopy. Whatever operative complications had not happened. Average operation times were 55 minutes. The total hospital days were 6 in average. Postoperative follow-up ranged from 0. 5 -3 years, no duodenal ulcer recurred. Conclusion The laparoscopy adopted in treating duodenal ulcer perforation is a procedure with minimal invasion and scarceness of postoperative complication; its clinical effectiveness is as good as laparotomized operation.

By means of correlative analysis of critical care air transportation management between China Air Force and United State Air Force,five areas of importance are discussed in this paper,such as the management model of strategies for air transportation,critical care team personnel composition,professional training mechanism,normalization and standardi-zation of operation process,and intensive application of new technology and equipment.

Adenocarcinoma ; classification ; pathology ; surgery ; therapy ; Cardia ; Chemotherapy, Adjuvant ; Esophageal Neoplasms ; classification ; pathology ; surgery ; therapy ; Esophagectomy ; methods ; Esophagogastric Junction ; surgery ; Gastrectomy ; methods ; Humans ; Neoplasm Staging ; Radiotherapy, Adjuvant ; Stomach Neoplasms ; classification ; pathology ; surgery ; therapy

0.05).In 25.28% of the population in the Second Songhua River region,the hair Hg content exceeded 1 mg/kg.In 21.85% and 16.67% of the population of Wujiazhan town and Songyuan city,the hair Hg content exceeded 1 mg/kg.Conclusion In the investigated area,the level of hair mercury shows a downtrend,but there still is health risk from mercury exposure.

Objective To explore the effects of percutaneous renal biopsy(PRB) on the etiological diagnosis and therapeutic regimen of patients with acute renal failure(ARF),so as to further improve the diagnostic and therapeutic levels of ARF.Methods From Nov.1992 to Dec.2007,176 patients were admitted in the Renal Division of Nanfang Hospital.All the patients matched the diagnostic criteria and were clinically diagnosed as ARF:within 48h the serum creatinine(SCr) ascended(≥26.5?mol/L) and increased by more than 50%,and the urine volume of less than 0.5ml/kg?h persisted in 6 hours.All the patients were undergone PRB and the clinical data were analyzed retrospectively.Results The final etiological diagnosis rate elevated from 64.2%(113/176) before PRB to 96.6%(170/176) after PRB.The coincidence of etiological diagnosis before and after PRB was 95.6%(108/113).Of 176 cases,170 were finally diagnosed as ARF,and the 6 remainders who were clinically misdiagnosed as ARF were finally diagnosed as chronic renal insufficiency(CRI).The therapeutic regimen for 83 patients was supplemented and for another 10 patients was modified after PRB,the total adjusted rate was up to 52.8%(93/176).After PRB,8 patients were finally diagnosed as IgA nephropathy,of them one case was specifically diagnosed as IgA protractedly leading to CRI,6 cases were ARF complicated with IgA,and one case was crescent formation induced by IgA nephropathy that leading to ARF.Conclusion PRB is a very useful technique for the etiological diagnosis of ARF on determining the therapeutic regimen and defining the prognosis.

Objective To investigate the pathology of nonpalpable breast lesions(NPBL),and evaluate the diagnostic value of ultrasound-guided mammotome(MMT)biopsy.Methods Between January 2005 and December 2007,254 patients with NPBL(328 lesions)diagnosed by ultrasonography received ultrasound-guided MMT biopsy.Among the patients,176 were diagnosed as having no breast mass or calcification by mammography.Results Of the 328 lesions,269(82.0%)were fibroadenomas,34 were(10.4%)cysts,3(0.9%)were intraductal papillomas,19(5.8%)were adenosis,and 3(0.9%)were early breast cancers.Among the 254 cases,251(98.8%)were benign without positives findings during follow-up.These patients received simple MMT,and 239(95.2%)of them were satisfied with the cosmetic outcome.The other 3(1.2%)patients who had malignancies were treated with modified mastectomy;none of them had residual tumors.The underestimate rate for NPBL was 0 in our cases.Of the 251 benign patients who received only MMT biopsy,239(95.2%)have satisfactory cosmetic results.Conclusions As an effective,minimally invasive,and rapid diagnostic technique for NPBL,ultrasound-guided MMT biopsy is an optimal tool for "one-step" operation.By using the method,underestimate risk can be decreased.

Objective: To explore the problem behaviors of high school students and its distribution among them. Methods: 1 739 middle school students in Beijing were collected as our sample (N=1739). Anonymous questionnaire of problem behavior of adolescents were used in this investigation. Results:1) the problem behaviors (general delinquency, cigarette smoking, and problem drinking) were significantly correlated with each other (r=0.28, 0.37, 0.39,P

Objective To discuss clinical and cytological features of cerebrospinal fluid (CSF) in meningeal careinomatosis (MC). Methods The data of clinic, imaging and CSF of 59 inpatients with MC were analyzed retrospectively. Results There were 91.5% (54 cases) of middle and elder aged in the group. Most of patients were at acute or subacute onset . Condition developed fast. 55 cases (93.2%) didn't feel fever primarily. Intracranial hypertension of headache (57 cases ),vomitted(45 cases ) and disc edema(21 cases ) ect. were predominant features. In 32 cases (54.2%), cranial nerves were involved. Optic never and abducent never damage appeared the most frequently. Oculomotor never, facial nerve and vestibulocochlear nerve were in the next. All of 59 cases had meningeal irritation sign, among them 31 cases (52.5%) with obvious sign. Tumor cells in cytological examination of CSF were found in all of 59 cases. Conclusions The clinical manifestations of MC has some certain features. Diagnosis of MC still relay on finding tumor cells in cytological examination of CSF.