Objective To investigate the protective effect of the new type of Tongxinluo (TXL) on blood-brain barrier impairment after cerebral ischemic/reperfusion injury in rats.Methods 78 male Sprague-Dawley rats were randomly divided into sham-operated group (n=6), operated group (n=36) and TXL-treated group (n=36), the latter two groups were divided into 0.5 h, 2 h, 6 h, 12 h, 24 h and 48 h groups (n=6, each) according to the time after cerebral ischemic/reperfusion for 2 h. The rats were pretreated with normal saline or TXL for 7 d. The middle cerebral artery occlusion/reperfusion (MCAO/R) rat model was established by the Longa occlusion method;The serum S100? protein concentrations of different point time were determined by ELISA method and infarct volumes were determined by 2,3,5-triphenyltetrazolium Chloride (TTC) staining method. Results (1) The serum S100? protein level in TXL-treated groups were significantly decreased compared with operated groups at 0.5 h, 2 h, 6 h,12 h and 24 h after MCAO/R (P

With the increasing trade amount of herbal medicine over the world,many foreign countries took non-tariff barriers such as technical regulations,technical standard and conformity assessment procedures,of which the most far-reaching impact of green trade barriers on the export of traditional Chinese medicine.We analyzed the three green trade barriers faced by the traditional Chinese medicine assessing international markets,and summarized the impacts of green trade barriers on the export of traditional Chinese medicine from the two aspects of positive and negative.In the end of this study,we came up with some strategies to deal with green trade barriers:to promote the establishment of standard system; to ensure the quality of traditional Chinese medicine products; to implement green production; to cultivate environmental awareness; to pursue international certification; and to enhance corporate brand image.

We overviewed the policies and regulations of traditional Chinese medicine of main Asian countries and summarized current situation of policies and regulations of traditional Chinese medicine in Asian markets.After in-depth analysis,we found that the opportunities lied in high public awareness,some Asian countries establishing special traditional medicine management agencies,special Pharmacopoeia of traditional Chinese medicine in Japan and South Korea,and high degree of acupuncture legalization.At the same time,traditional Chinese medicine also faced with the challenges such as strict technical standard and TBT barriers.In the end of this paper,we came up with some development countermeasures when traditional Chinese medicine accessing Asian markets,such as improving technical standards,establishing an anti-technical barriers to trade service system,encouraging technological innovation,and strengthening domestic and international exchanges and cooperation.

Objective To explore the clinical features and treatment of children with acute severe viral myocarditis.Methods The clinical data of presentation,diagnosis,therapy and prognosis of children who were admitted in our hospital from Jan 2005 to Jan 2012 with acute severe viral myocarditis(severe myocarditis group) were analyzed retrospectively.Twenty-three cases of normal healthy children in the same period were selected as control group.The levels of serum cardiac troponin(CTn)-Ⅰ and N-terminal pro-brain natriuretic peptide(NT-proBNP) were detected by ELISA method,the changes of left ventricular ejection fraction and left ventricular fraction shortening were understood by color doppler echocardiography.Results The level of CTn-Ⅰin severe myocarditis group was significantly higher than that of control group,the difference was statistically significant [(18.67 ± 12.31) ng/ml vs (0.02 ±0.01) ng/ml,P ＜0.05].Compared with the acute phase,the level of CTn-Ⅰshowed a trend of gradual decline in 7 d [(0.55 ±0.24) ng/ml],basic close to normal in 14 d [(0.06 ±0.03) ng/ml] (P ＜0.05).The level of NT-proBNP increased significantly in severe myocarditis group compared with control group [(3 067.26 ± 902.79) pg/ml vs (80.04 ± 17.79) pg/ml,P ＜0.05].Compared with acute phase,the levels of NT-proBNP were closed to normal in 7 d [(648.63 ±342.37) pg/ml] and 14 d [(213.58 ± 129.51) pg/ml] (P ＜ 0.05).The left ventricular ejection fraction [(52.63 ± 6.98) ％ vs (71.39 ± 2.41) ％] and left ventricular fraction shortening [(32.1 ± 2.97) ％ vs (40.04 ± 2.31) ％] in severe myocarditis group were significantly lower than those in control group (P ＜ 0.05).Conclusion Acute severe viral myocarditis of children was characterized by rapid onset,severe illness and high mortality.Early use of adrenal cortical hormone and gamma globulin under the comprehensive treatment and application temporary pacemaker can help patients to recover from the disease.

As a kind of additive in feed of monogastric animals, the application of natural phytase is limited due to its disadvantages. In this paper, the strategies of phytse reform was introduced. Furthermore, the research and progress on protein engineering of feed phytase was reviewed, including phytase over-expression, phytase thermostability, catalytic efficiency and optimum pH.

To explore the relationships between traditional Chinese medicine (TCM) constitutional types and overweight or obesity so as to provide evidence for adjusting constitutional bias and preventing and treating obesity.

Aged ; CD5 Antigens ; metabolism ; Chromosomes, Human, Pair 11 ; Chromosomes, Human, Pair 14 ; Cyclin D1 ; metabolism ; Diagnosis, Differential ; Female ; Humans ; Liver Neoplasms ; genetics ; metabolism ; pathology ; surgery ; Lymphoma, B-Cell ; metabolism ; pathology ; Lymphoma, Follicular ; metabolism ; pathology ; Lymphoma, Mantle-Cell ; genetics ; metabolism ; pathology ; surgery ; Pseudolymphoma ; metabolism ; pathology ; Translocation, Genetic

Angiopoietin-1 ; chemistry ; physiology ; Angiopoietin-2 ; chemistry ; physiology ; Angiopoietins ; physiology ; Animals ; Cardiovascular Diseases ; therapy ; Embryonic and Fetal Development ; Humans ; Neoplasms ; blood supply ; Receptor, TIE-2 ; chemistry ; physiology ; Signal Transduction

Adolescent ; Child ; Child, Preschool ; Electrocardiography ; Female ; Fever ; complications ; Hemorrhagic Fever with Renal Syndrome ; blood ; complications ; pathology ; Humans ; Hypergammaglobulinemia ; blood ; Immunoglobulin M ; blood ; Male ; Pain ; complications

BACKGROUND:With the development of modern pathological techniques, the misdiagnosis rate has been reduced remarkably, but special stains are still the most important method for pathological diagnosis. OBJECTIVE:To compare the advantages and disadvantages of different special stains used for observing the structure of human lumbar facet joints. METHODS:The specimens of facet joint cartilage at L4/5 level were collected from patients undergoing lumbar surgery, and then stained with hematoxylin-eosin, safranin O, toluidine blue, Masson, and saranin-O-fast green for structure observation. RESULTS AND CONCLUSION:The structure of the articular cartilage could be observed clearly through hematoxylin-eosin, toluidine blue, and saranin-O-fast green staining. The cartilage surface, tidemark, and subchondral bone were shown by the hematoxylin-eosin staining, with the presence of violet chondrocyte nuclei. Safranin-O-fast green staining showed the four layers of the cartilage clearly, including the shallow layer (cartilage surface), middle layer (spherical cells arranged in disorder), columnar cell layer (large and multinucleated chondrocytes arranged neatly), tidemark, subchondral bone layer; and the cartilage matrix was reddish uniformly, the subchondral bone was green, and the cartilage and bone tissue showed a striking contrast. The cartilage structure was unclear in toluidine blue staining, with clear nuclei and almost no coloring cytoplasm, but the matrix appeared with slight purplish blue. Safranin O staining showed that the cartilage was red, which had no obvious boundary with the cartilage matrix, and chondrocytes were stained lightly. Masson staining showed clear collagen fibers, but the structures of the cartilage and subchondral were obscure. To conclude, safranin-O-fast green staining can achieve the best results, followed by hematoxylin-eosin staining and Masson staining in turn.