Objective: To investigate the therapeutic effects of radioisotope labeled Ku70 antisense oligodeoxynuclecotide (ASODNs) on thyroid carcinoma implanted in nude mice and the related mechanism. Methods: The Ku70 ASODNs labeled with 131I was used to treat the tumor-bearing mouse model derived from thyroid carcinoma TT cells. The tumor-forming rate, mortality rate and tumor growth were observed and calculated after treatment with 131I-ASODNs, 131I-Na, ASODNs or NS (normal saline). Annexin V/PI assay was used to examine the apoptosis of tumor cells by flow cytometry. Western blotting analysis was performed to determine the protein expressions of Ku70, proliferating cell nuclear antigen (PCNA, a cell proliferation marker) and Bcl-2(a cell apoptosis marker). Results: After treatment with 131I-ASODNs, the Ku70 protein level was down-regulated in the tumor tissues and the growth of tumor was inhibited. The tumor volume, tumor-forming rate and mortality rate were significantly decreased in 131I-ASODNs group than in the NS control group (P<0.01). The tumor volume of 131I-ASODNs group was also significantly smaller than that in the 131I-Na group (P<0.05); the apoptosis rate of 131I- ASODNs group (35.6%) was significantly higher than that of the ASODNs group (10.4%), NS group (9.2%) and 131I-Na group (26.6%) (P<0.05). Further investigation found that PCNA and Bcl-2 protein levels in 131I-ASODNs group were lower than those in NS and ASODNs groups. Conclusion: The Ku70 131I-ASODNs can effectively inhibit the growth of thyroid carcinoma and promote apoptosis of TT tumor cells, which might be related to the down-regulation of Ku70 and the changes of PCNA and Bcl-2 signal pathway.

Objective To study the effects of Exenatide on JAK1/STAT1 expression in the islet tissues and apoptosis of pancreatic B-cell in type 2 diabetic rat model with insulin resistance. Methods Insulin resistant type 2 diabetic rat model was induced by combined treatment with high-fat diet and low-dose streptozotocin. The model rats were treated with Exenatide (Ex), metformin (Met) or normal saline (NS); the serum glycosylated hemoglobin (HbAu), fasting insulin (FINS), and fasting plasma glucose (FBG) were observed and the insulin sensitivity index was calculated to evaluate the therapeutic effects of Exenatide on insulin resistance. Western blotting analysis was performed to detect the protein expression of JAK1/STAT1 in the islet tissues. Annexin/PI staining assay was used to evaluate B-cell apoptosis induced by oxidative stress. Results Ex group showed a significantly elevated ISI and a reduced HbA1c level compared with NS group (P<0.05). JAK1/STAT1 expression , and H 2O2-induced B-cell apoptosis rate were significantly lower in Ex group than in Met and NS groups (P < 0.01). Conclusion Exenatide can improve insulin sensitivity and inhibit B-cell apoptosis, probably by regulating the expression of JAK1/STAT1.

[Objective]To develop a standard closed experimental fracture model in mouse femur.[Method]A 0.45 mm stainless-steel rod was introduced into the medullary cavity and the pre-nailed femur shaft was fractured by an impact device,which resulted in a reproducible transverse fracture pattern.The fractures were examined using X-ray Micro-CT,and histological method.[Result]Transverse fractures were created in 91.67% of the experimental mice,and the fracture healing was typical secondary fracture healing.[Conclusion]The standard closed fracture model in this experiment,whose healing was typically secondary fracture healing,can be obtained easily and used in study of fracture.

Objective To prospectively study the effects of different fluid resuscitation on kidney function in pediatric patients with sepsis.Methods One hundred and eighteen pediatric patients with sepsis shock who needed fluid resuscitation in Intensive Care Unit,Zhengzhou Children′s Hospital from June 2012 to June 2015 were selected, and they were randomly divided into group A,B and C by random number in the table.The patients received fluid resus-citation of hydroxyethyl starch,50 g/L albumin and 9 g/L saline.After that,neutrophil gelatinase associated lipocalin (NGAL),α-1 microglobulin,urea nitrogen,creatinine,urine volume,the incidence of acute kidney injury (AKI),and the renal replacement therapy rate accepted by the patients were recorded.Then all the data were analyzed with SPSS 19.0 software.Results Serum levels of NGAL and α-1 microglobulin in group A [(103.50 ±1.23)μg/L,(3.32 ± 0.20)mg/L]were higher than those in group B [(93.54 ±1.42 )μg/L,(2.71 ±0.18 )mg/L]and group C [(79.57 ±1.54)μg/L,(2.08 ±0.14)mg/L],which were statistically different(all P <0.05 );serum levels of NGAL and α-1 microglobulin in group B were higher than those in group C,which were statistically different(all P <0.05).Serum levels of urea nitrogen and creatinine in group A[(5.23 ±0.34)mmol/L,(112.00 ±7.12)μmol/L] were higher than those in group B [(3.56 ±0.31)mmol/L,(77.25 ±5.34)μmol/L]and group C[(2.95 ±0.42) mmol /L,(72.12 ±6.34)μmol/L],which were statistically different (all P <0.05);serum levels of urea nitrogen and creatinine in group B were higher than those in group C,which were not statistically different(P >0.05).The urine volume in group A [(0.50 ±0.12)mL/(kg·h)]was less than that in group B[(0.90 ±0.23)mL/(kg·h)]and group C[(1.30 ±0.14)mL/(kg·h)],which was statistically different(all P <0.05);the urine volume in group B was less than that in group C,which was not statistically different(P >0.05).In three groups,there were 13 cases (34.2%)with AKI in group A,9 cases(23.1%)with AKI in group B,and 8 cases(19.5%)with AKI in group C, the incidence of AKI in group C was less than that in group B and group C,which was statistically different(χ2 =12.74,10.43;all P <0.05).All the groups all had children accepted renal replacement therapy,there were 8 cases (21.1%)in group A,7 cases(17.9%)in group B,and 6 cases (14.6%)in group C,and the cases who accepted renal replacement therapy rate in group C were less than those in group A and group B,which was statistically different(χ2 =11.36,8.73;all P <0.05).Conclusions The adverse effect of 9 g/L saline on kidney function is less than hydrox-yethyl starch and 50 g/L albumin,and hydroxyethyl starch has more adverse effects on kidney.Thus,9 g/L saline is a better option for fluid resuscitation in pediatric sepsis patients.

Objective To investigate the role of hypoxia inducible factor-1α (HIF-1α) and von Hippel-Lindau (VHL) in murine endochondral ossification. Methods The knockout of HIF-1α or VHL gene in murine osteoblasts was accomplished by conditional knockout technique at 4th, 8th and 12th week, and the differences between wild-type group and knock-out group in endochondral ossification were detected by HE staining, micro-CT scanning, trabecular bone area measurement, calcium content measurement, tetracycline fluorescence labeling, Real-time PCR and Western blotting. Results After knockout of HIF-1α gene in osteoblasts, the expression of vascular endothelial growth factor ( VEGF) reduced, the rate of new bone formation stepped down, the content of calcium became less, and the trabecular bone volume decreased (P <0.05) . After knockout of VHL gene in osteoblasts, the expression of VEGF increased, the rate of new bone formation stepped up, the content of calcium became more, and the trabecular bone volume was promoted (P < 0.001). Conclusion During murine endochondral ossification, VHL/HIF-1α signal pathway promotes angiogenesis through the stimulation of VEGF expression, which subsequently accelerates osteogenesis.

Objective To investigate the expression difference of vascular endothelial growth factor (VEGF)between lung cancer and lung benign disease,and its relation to clinical characteristics and prognosis of non-small cell lung cancer(NSCLC),and to research the possible role of VEGF in the growth of lung can- cer.Methods The expression of VEGF and microvascular density(MVD)were detected in 60 lung cancer tissues and 30 lung benign disease tissues after operation by immunohistochemical method and put up statisti- cal analysis.Results The positive rate of VEGF expression(63%)and MVD(45.13?10.27)in lung cancer tissues were both higher than those in lung benign disease tissues(27%,33?6.49)(P

Objective To evaluate the efficacy and safety of direct stenting technique in emergent percutaneous coronary intervention(PCI)for pa?tients with acute myocardial infarction(AMI). Methods Totally 460 consecutive patients with acute myocardial infarction who underwent emergent PCI during August 2010 to July 2013 were involved,among whom 346 patients were assigned to the control group(traditional balloon dilation and stenting),114 patients were assigned to the experiment group(direct stenting). The safety and efficacy of direct stenting technique was observed. Results In the control group,7 cases had no reflow during operation,2 cases had reflow after the pre?expansion and 5 cases after stent implanta?tion,no similar cases in the experiment group(P=0.125 9). The follow?ups showed the control group had 2 cases of major adverse cardiac events including 1 case of acute stent thrombosis who required urgent revascularization,and 1 case of cardiac death at four days after operation;the experi?ment group had 1 case of stent thrombosis,there was no statistically significant difference(P=0.730 7). Conclusion Direct stenting technique may be performed selectively for certain coronary diseases when performing emergent PCI for AMI.

Objective:To explore the prevalence rate of suicidal ideation and its risk and protective factors in military personnel.Methods:A total of 2071 military personnel were investigated by stratified random cluster sampling in May to August of 2015.The Question 7 of Beck Depression Scale (BDI) was used to test suicide ideation (item 7 score ≥ 1 was dovetailed in suicide ideation group).Family,social demographic and occupational factors were assessed with the self-made general characteristics questionnaire.The data were analyzed with multiple stepwise logistic regression.Results:There were 382 (18.4％) military personnel whose scores of item 7 were ≥1.Family history of suicide (OR =42.97,95％ CI =29.14-63.35),surrounding suicide (OR =2.46,95％ CI =1.75-3.46),drinking (OR =1.64,95％ CI =1.31-2.07) and level of education (OR =1.38,95％ CI =1.01-1.90) were risk factors of suicidal ideation.Protective factors were (OR =0.60,95％ CI =0.50-0.72) and station (OR =0.15,95％ CI =0.03-0.64).Conclusion:It suggests that in suicidal prevention for military,more attention is deserved paying to those military personnel who have family history of suicide,surrounding suicide,drinking habit,and are stationed in remote areas and air force.

Adolescent ; Female ; Humans ; Patellar Dislocation ; physiopathology ; surgery ; Patellar Ligament ; injuries ; physiopathology ; surgery ; Range of Motion, Articular ; Reconstructive Surgical Procedures

Animals ; Cells, Cultured ; Male ; Phenols ; toxicity ; RNA, Messenger ; genetics ; Rats ; Rats, Sprague-Dawley ; Sertoli Cells ; drug effects ; metabolism ; Steroidogenic Factor 1 ; genetics ; metabolism