Cardiac Pacing, Artificial ; Electrodes, Implanted ; Female ; Humans ; Middle Aged ; Postoperative Complications ; etiology ; Subclavian Artery ; injuries ; Treatment Outcome

Myocardial Infarction ; physiopathology ; Myocardium ; Myocytes, Cardiac ; physiology ; Regeneration ; Tissue Engineering

Atrial Fibrillation ; therapy ; Catheter Ablation ; adverse effects ; Humans ; Male ; Middle Aged ; Phrenic Nerve ; injuries ; Trauma, Nervous System ; etiology

Adolescent ; Defibrillators, Implantable ; Female ; Humans ; Long QT Syndrome ; therapy

Adult ; Catheter Ablation ; methods ; Humans ; Male ; Sinus of Valsalva

Objective　To investigate the diagnose and therapy of patients with smooth muscle tumors (SMTs) of gstrointestinal tract (GI). Methods　A retrospective analysis was made on the clinical and pathological data of 92 patients with SMTs of GI admitted from 1982 to 1999 in Nanfang Hospital. Results　Location of the lesion: 41 cases in stomach, 44 in small intestine, and 7 in large bowel. Adjuvant examinations: BUS was performed on 63 cases with positive findings in 31; CT on 27 cases with positive findings in 20; DSA on 29 cases with positive findings in 27; barium series on 45 with positive findings in 28; and small intestinoscopy on 11 cases with pasitive findings in 2. Conclusions　DSA is the most effective method in diagnoses of SMTs of GI, especially for small intestinal leiomyoma. The primary treatment of GI-SMTs is surgical resection.

Objective To study the relationship between salt consumption and hypertension in Chinese people,and provide basic information for developing intervention strategies.Method The data of 45 349 chinese residents aged 15 yrs and above from 2002 China National Nutrition and Health Survey was used.Results The hypertension prevalence was 18.2%.The prevalence of residents with high blood pressure value was 36.0%,which was 20.4% and 16.2% among men and women,respectively. Hypertension prevalence among people living in urban was higher than their counterparts living in rural.The prevalence of hypertension increased with salt consumption.As compared to people who consumed less than 6 g salt per day,after relative confounding factors adjusted,the prevalence ratio was 1.09,1.14 and 1.28 times,respectively,among people who averagely consumed 6～12 g,12218 g,and≥18 g salt per day,which was 1.13,1.11 and 1.30 times,respectively,among employment population.Conclusion The hypertension prevalence of chinese residents is quite high.There is significant relationship between salt consumption and hypertension.It is very important to strengthen the health education for preventing and controlling hypertension in Chinese residents.

Objective： The current study was designed to investigate the expression of glutathione s-transferase π ( GST-π ) and its clinical significance in human primary transitional cell carcinoma(TCCs) of bladder. Methods： A total of 107 human primary bladder TCCs of the previously untreated patients were analyzed for expression of GST-π and P-glycoprotein (Pgp) by SP immunohistochemistry (IHC). Results： The GST-π positive staining were divided into three groups： single cytoplastic staining, single nuclear staining, both cytoplastic and nuclear staining. GST-π were present in 72.9％ generally, GST-π cytoplastic staining (single cytoplastic, both cytoplastic and nuclear staining) were found in 58.9％ of the samples, 46.7％ of them were positive nuclear staining (single nuclear, both cytoplastic and nuclear staining). GST-π general positive staining and cytoplastic positive staining were significantly correlated with grade, stage and recurrence after post-operative intravesical chemotherapy. Both of them were also strongly related to the expression of Pgp. While GST-π nuclear positive staining was related to stage but not with grade and recurrence. Conclusions： Overexpression of GST-π can be used as a predictive marker of post-operative intravesical chemotherapy in TCCs. Furthermore increased cytoplastic staining is a better predictor of drug resistance while increased nuclear staining might be associated with progression in TCCs.

The axon guidance molecule Slit is a secreted glucoprotein which is conserved during evolution. Slit has been implicated in regulating a variety of life activities, such as axon guidance, neuronal migration, neuronal morphological differentiation, tumor metastasis, angiogenesis and heart morphogenesis. Slit function mainly depends on the binding of its LRR-2 domain to the Ig1 domain of Roundabout (Robo) receptor, meanwhile Slit function is also mediated by a range of signaling molecules, including the heparan sulfate proteoglycans (HSPGs), GTPase-activating proteins (GAPs), tyrosine kinase Abelson, calcium ions, MicroRNA-218 and other axon guidance molecules. Several transcription factors, including Single-minded, Irx and Midline, were shown to regulate slit expression. In addition, multiple Slit isoforms exist as a consequence of alternative spliced transcripts. The research on guidance mechanism of Slit will facilitate the understanding of molecular mechanism underlying neural networks formation in the process of neural development and regeneration. Meanwhile, the studying of Slit guidance mechanism could promote the prevention and treatment of human neurological diseases and cancer metastasis.
Animals ; Axons ; metabolism ; physiology ; Cell Movement ; physiology ; Drosophila Proteins ; physiology ; Gene Expression Regulation ; Humans ; Intercellular Signaling Peptides and Proteins ; genetics ; physiology ; Nerve Tissue Proteins ; genetics ; metabolism ; physiology ; Neurons ; cytology ; Receptors, Immunologic ; metabolism

Objective To obtain an acellular matrix from bovine pericardial tissue, as a scaffold for tissue engineering of heart valves or cardiovascular patching applications. Methods A four step detergent and enzyme link extraction procedure was practiced in this study. The mechanic properties of the acellular matrix were evaluated, and the components were analyzed biochemically. Results HE staining confirmed the removal of cells and Von Gieson staining showed the integrality of collagen and elastin. Biochemical analysis demonstrated the retention of collagen and some glycosaminoglycans while the percentage of the soluble proteins reduced slightly. The tissue fracture strength and shrinkage temperature made no significant differ ence after extraction. Conclusions The extraction process is effective to remove cells from bovine pericardial tissue while maintains its mechanical strength. This approach may eventually lead to a scaffold for heart valves and cardiovascular patching applications in tissue engineering.