Objective:To observe the therapeutic effect of acupuncture-moxibustion at different intervals on persistent allergic rhinitis. Methods: A total of 90 patients conforming to the inclusion criteria were randomized into three groups named A, B and C by randomized block method. Patients in all three groups received the same treatment of acupuncture and herbal cake-partitioned moxibustion at the same acupoints, while the treatment frequency was different. Patients in group A received the treatment once a week, group B twice a week and group C three times a week, and all the treatment lasted for 4 weeks. The total nasal symptom score (TNSS), total ocular symptom score (TOSS) and Sino-nasal outcome test-20 (SNOT-20) were evaluated before and after treatment. The self-rating score of symptoms was evaluated during treatment and 2 weeks after treatment. Results: The total effective rate was 80.0% in group A, 93.3% in group B, and 100.0% in group C. The total effective rate in group A was statistically different from that in group B and group C (both P＜0.05), but there was no significant inter-group difference in total effective rate between group B and group C (P＞0.05). After treatment, scores of TNSS, TOSS and SNOT-20 in all three groups dropped significantly, and statistically different from those before treatment (all P＜0.05); between-group comparison showed group B and group C decreased more obvious than group A (all P＜0.05). In self-rating score of symptoms, there were no inter-group statistically significant differences in the first 3-week treatment (all P＞0.05); after 4-week treatment, the score in group A was higher than that in group B and group C, and showed statistical significant (both P＜0.05); at 2 weeks after treatment, the score in group A was higher than that in group B and group C based on an everyday record, showing statistical significance (both P＜0.05). At the 11th day after treatment, the score in group B was higher than that in group C (P＜0.05). There were no significant differences between group B and group C at other time points (all P＞0.05). Conclusion: All three protocols are effective for allergic rhinitis. With the increase of treatment frequency, the therapeutic efficacy with a treatment frequency of twice a week and three times a week is superior to that of once a week. Frequency of three times a week has a better long-term effect than once and twice a week, together with the least fluctuation of symptoms.

OBJECTIVE: A systematic study of Laggera pterodonta was performed in order to identify its bioactive compounds. METHODS: Solvent partition and column chromatography used to isolate and purify Laggera pterodonta compounds. The isolated compounds were further elucidated by high field NMR spectroscopy. RESULTS: Fifty-two compounds were separated and the structures of 39 compounds were elucidated. Twenty-four compounds have not been previously reported. For example, the structure of compound 19, a previously undetected compound was elucidated as 4gamma, 9alpha,11-triol-enantio-eudesmane using both 1D and 2D NMR spectroscopy. Major secondary metabolites of the Laggera pterodonta included eudesmane-type sesquiterpenes and flavone derivatives. CONCLUSION: A systematic study of a yunnan herbal medicine Laggera pterodonta resvealed several novel compounds that may have clinical significance. Further in vivo animal studies should be performed to assess their bioactive role.

OBJECTIVETo establish the median of serum markers for second trimester screening in Qingdao region and to assess the influence of median correction on the performance of screening.

METHODSMaternal serum alpha-fetoproteins (AFP), human chorionic gonadotrophin, free beta subunit (β -HCG) and unconjugated oestriol (uE3) were assayed for prenatal screening of 18 188 singleton pregnancies at 15-20(+ 6) weeks gestation from January 2009 to July 2010. The median of serum markers was calculated based on above results and applied for risk estimation in screening for fetal aneuploidy from August 2010 to March 2011. The screening performance, specified in terms of detection rates (DRs), false positive rates (FPRs) and odds of being affected given a positive result (OAPR) were compared between the two groups. The risks of 45 affected pregnancies detected during the study were estimated with both Caucasian and corrected medians.

RESULTSThe average level of AFP in local pregnancies was similar to that of the Caucasian population, whilst β -HCG and uE3 were respectively 11% and 33% higher than those of Caucasians. The multiple of median (MoM) value was between 0.94 and 1.02 for the dataset based on the corrected median. At a cut-off of l in 270, FPR has decreased from 5.2% to 4.9%, and DR of Down syndrome has increased from 60% to 69.2%, and OAPR has increased from 1:79 to 1:59 when evaluating risk based on the corrected median. For the 45 affected pregnancies, three Down syndrome pregnancies could be missed because their risk estimates were lower than the cut-off level based on Caucasian median.

CONCLUSIONIt is useful to establish and apply population and laboratory-specific medians in order to improve the performance of prenatal screening and diagnosis.

Adult ; Biomarkers ; blood ; Estriol ; blood ; Female ; Humans ; Lindane ; blood ; Pregnancy ; Pregnancy Trimester, Second ; Prenatal Diagnosis ; methods ; alpha-Fetoproteins ; analysis

OBJECTIVETo analyze the impact of transurethral resection of the prostate (TURP) on erectile function and the factors influencing postoperative erectile function.

METHODSAltogether 64 male patients aged 53 -75 (mean 66.5) years underwent TURP for prostatic hyperplasia. Before and 3 months after surgery, we observed the nocturnal penile tumescence of the patients and analyzed their scores on the 5-item version of the International Index of Erectile Function (IIEF-5) and the Self-Rating Anxiety Scale (SAS).

RESULTSIntraoperative prostatic capsule perforation and postoperative stress were significantly related to postoperative erectile dysfunction (P < 0.05). The mean score of IIEF-5 was significantly decreased (P < 0.01) while that of SAS remarkably increased (P < 0.01) after TURP as compared with those before surgery. The frequency of nocturnal penile tumescence was reduced at 3 months after surgery, but with no statistically significant difference.

CONCLUSIONIntraoperative prostatic capsule perforation and postoperative stress obviously affect postoperative erectile function.

Aged ; Erectile Dysfunction ; etiology ; Humans ; Male ; Middle Aged ; Prostatic Hyperplasia ; surgery ; Transurethral Resection of Prostate ; adverse effects

Adult ; Aged ; Aged, 80 and over ; Decompression, Surgical ; Female ; Fractures, Bone ; complications ; surgery ; Humans ; Leg Injuries ; complications ; surgery ; Male ; Middle Aged

OBJECTIVETo explore a new method to reconstruct the defect of the distal foot.

METHODSA distally based dorsum pedis island flap pedicled with the first dorsal metatarsal artery was designed and transferred to the defect of the distal foot.

RESULTSFive patients were treated with this flap, which ranged from 2 cm x 4 cm to 6 cm x 7 cm in size. Four flaps survived completely, one flap had marginal necrosis and healed after conservative therapy.

CONCLUSIONSThe reverse first dorsal metatarsal artery flap, with good blood circulation and easy manipulation, is a good option for the defects of the distal foot.

Adolescent ; Adult ; Child ; Female ; Foot Injuries ; surgery ; Humans ; Male ; Metatarsus ; blood supply ; Middle Aged ; Skin Transplantation ; methods ; Surgical Flaps ; blood supply ; Young Adult

OBJECTIVEThe purpose of this study was to search for a perfect treatment for the complications after polyacrylamide hydrogel injection for augmentation mammaplasty.

METHODS48 patients who accepted polyacrylamide hydrogel injection for augmentation mammaplasty were included in this study. Operations were performed for the complications of polyacrylamide hydrogel injection. The clinical data were analyzed and the experience in the treatment of the complications was summarized.

RESULTSAll patients were satisfied with the result of the operation. The B-ultrasonic examination showed that no visible polyacrylamide hydrogel remained in the cavity.

CONCLUSIONThe best therapy for the complications of polyacrylamide hydrogel injection was removing of the polyacrylamide hydrogel and the pathologic tissue and irrigation of the cavity.

Acrylic Resins ; administration & dosage ; Adult ; Female ; Humans ; Infection ; etiology ; surgery ; Injections ; Mammaplasty ; adverse effects ; methods ; Pain ; etiology ; surgery ; Postoperative Care ; Postoperative Complications ; etiology ; surgery ; Surgical Procedures, Operative ; standards ; Treatment Outcome

OBJECTIVETo improve the survival of mesenchymal stem cells (MSCs) for prefabrication of an osteo-musculo-cutaneous flap.

METHODSIn a mouse model, the compound of MSCs and p(3HB-co-3HH) were embeded in the latissimus dorsi muscle as an experimental group and the muscle pocket of the buttock as the control. The examinations of the HE staining, hybridization in situ of osteonectin mRNA and Von kossa staining were used to evaluate the results.

RESULTSThe expression of osteonectin mRNA and the Von kossa staining showed that the latissimus dorsi muscle group was superior to the control in 2 weeks and 4 weeks after the surgery in vivo.

CONCLUSIONThe results indicate that the above-mentioned technique may be a good alternative for the prefabrication of the osteo-musculo-cutaneous flap.

Animals ; Fascia ; transplantation ; Histocytochemistry ; methods ; In Situ Hybridization ; Male ; Mice ; Mice, Nude ; Models, Animal ; Osteonectin ; genetics ; metabolism ; RNA, Messenger ; genetics ; metabolism ; Staining and Labeling ; Stem Cell Transplantation ; methods ; Surgical Flaps ; blood supply ; Tissue Engineering ; methods

OBJECTIVETo analyze and optimize the gene rearrangement primers of different frame regions (FR) of immunoglobulin heavy chain (IgH) genes by bioinformatic methods and explore the application of these primers in the detection of paraffin-embedded lymphoma tissues.

METHODSThree pairs of primers from IgH FR1, FR2 and FR3 regions (P1c, P2A and P31, respectively) were selected as the B cell gene rearrangement primers after comparison of the gene fragments in 44 IgH variable and 6 joining regions. Using one pair of T cell receptor (TCR) gamma primer as the T cell gene rearrangement primer, 101 histopathologically confirmed lymphoproliferative samples including 80 B cell lymphomas, 14 T cell lymphomas, and 7 reactive proliferative lymph nodes were examined by PCR for gene arrangement. The DNAs from DG75 and Jurkat cell lines were used as the positive controls for B and T cell lymphoma, respectively, with those from reactive proliferative lymph nodes as the negative control.

RESULTSThe positivity rates of IgH primers (P1c, P2A and P31) in the 80 B cell lymphomas were 37.5% (30/80), 52.5% (42/80) and 70.0% (56/80), respectively, and only one of the 14 T cell lymphoma cases was positive for the primers, suggesting significant differences in the detection rates of B cell lymphomas by the 3 primers. The detection rate was increased to 83.9% by combining the results by P31 and P2A primers. No positivity was found in the proliferative reaction tissues.

CONCLUSIONPrimers from IgH FR3 region genes are more sensitive than that from the FR1 and FR2 regions in the detection of gene rearrangement in paraffin-embedded lymphoma tissues. The detection rates can be increased by combining the results with the primers for IgH FR3 with that of FR2.

DNA Primers ; Gene Rearrangement, B-Lymphocyte, Heavy Chain ; genetics ; Humans ; Immunoglobulin Heavy Chains ; genetics ; Lymphoma, B-Cell ; diagnosis ; genetics ; pathology ; Lymphoma, Non-Hodgkin ; diagnosis ; genetics ; pathology ; Lymphoma, T-Cell ; diagnosis ; genetics ; pathology ; Male ; Paraffin Embedding

OBJECTIVETo develop the first grade standard analytical reference material of bovine blood lead.

METHODSAccording to standards and technology specification of primary standard reference material (JJG1006-1994), ISO 17511, and volume-effect relationship, a bovine blood lead model was developed by adding acetate lead in the feed in dose of 2-5 mg x kg(-1) x d(-1). Cow blood was collected when blood lead concentration went up to low, medium and high concentration range (90-100, 190-200, 280-300 microg/L). Blood sample was kept in tube and frozen after irradiation. The samples were tested for homogeneity and stability. ID-MS method was used to measure the lead concentration through comparison with two standard lead samples from the USA NIST SRM 955b.

RESULTSSamples of the three lead concentrations showed uniformity by single factor analysis of variance (F = 0.61, 1.64, 0.28, respectively, P > 0.05) . After 14 months monitoring, the RSD was 0.85%, 1.05% and 0.49% (t = 0.787, 1.132, 0.854, respectively, P > 0.05). The characteristic and indefinite values were 102.4 +/- 5.5; 181.2 +/- 4.0; 304.5 +/- 3.9, respectively (unit: microg/L). The reproduction of the two USA NIST SRM 955b samples further showed our methods and procedures were correct. Our sample was stabile for four days at room temperature.

CONCLUSIONThe standard reference material of bovine in our research had reached the national standard material requirements.

Animal Feed ; Animals ; Blood Chemical Analysis ; standards ; Cattle ; Lead ; blood ; Male ; Models, Animal ; Reference Standards