Objective To discuss the minimally invasive skills in the treatment of choledocholithiasis.Methods A retrospective analysis was made to sum up the minimally invasive methods in the treatment of choledocholithiasis,reinforcing sufficient cognition to the treatment of the disease.Results The minimally invasive methods in the treatment of choledocholithiasis included endoscopic sphincterotomy,endoscopic papillaryballoon dilation,fibercholedochoscopy,laparoscopic common bile dnctexploration and laparoscopy combined with endoscopy in treating choledocholithiasis.Conclusion The conception of the treatment of choledocholithiasis was renewed by djfferent minimally invasive skills.With the development of the medical apparatus and instruments,the minimally invasive skills will play an important role in the treatment of choledocholithiasis.

Genome editing is defined as highly-effective and precise modification of cellular genome in a large scale. In recent years, such genome-editing methods have been rapidly developed in the field of industrial strain improvement. The quickly-updating methods thoroughly change the old mode of inefficient genetic modification, which is "one modification, one selection marker, and one target site". Highly-effective modification mode in genome editing have been developed including simultaneous modification of multiplex genes, highly-effective insertion, replacement, and deletion of target genes in the genome scale, cut-paste of a large DNA fragment. These new tools for microbial genome editing will certainly be applied widely, and increase the efficiency of industrial strain improvement, and promote the revolution of traditional fermentation industry and rapid development of novel industrial biotechnology like production of biofuel and biomaterial. The technological principle of these genome-editing methods and their applications were summarized in this review, which can benefit engineering and construction of industrial microorganism.
Biotechnology ; Fermentation ; Genetic Engineering ; methods ; Genome, Microbial ; Industrial Microbiology

Child ; Common Cold ; diagnosis ; therapy ; Communicable Disease Control ; methods ; organization & administration ; standards ; Communicable Diseases ; diagnosis ; therapy ; Humans ; Patient Education as Topic ; Respiratory Tract Infections ; therapy ; Risk Factors ; Severe Acute Respiratory Syndrome ; diagnosis ; therapy ; Virus Diseases ; diagnosis ; therapy

China ; Humans ; Measles ; prevention & control ; Measles Vaccine ; Vaccination

Objective To analyze risk factors of vascular disease in the lower limbs in type 2 diabetes patients.Meth- ods Investigating the lower limb vein of 80 type 2 diabetes patients with Siemens G-50 color Doppler ultrasonograph, and recording their age,sex,course of disease,and blood pressure,at the same time,take some tests as follows: FBG,P2hBG,FIN,C-peptide HbA_1c,Tch,UAE.Results Compared to the control group,blood pressure, FBG,P2hBG,Tch,TG,HbA_1c,Tch,UAE of the type 2 diabetes patients with lower limb vascular disease,are all increased significantly(P

The clonal proliferations of multiple myeloma cells consist of several signaling pathways.It has been shown that cyclic adenosine monophosphate (cAMP) signaling pathway,an important intra-cellular messenger delivery system,is related to abnormal proliferation and apoptosis of malignant lymphoid cells including myeloma cells.Manipulating the cAMP signaling pathway could induce cell cycle arrest and apoptosis of various malignant lymphoid cells including myeloma cells,which involve mitochondrial-dependent apoptosis and cAMP-modulated changes of intra-cellular mediators.In-depth researches and analysis of cAMP-induced apoptosis in myeloma cells would provide potential targets and possible therapeutic means in the treatment of multiple myeloma.

Objective To analyze retrospectively the result of nonspecific mastitis diagnosed by mammography and color Doppler's ultrasonic, and to improve the diagnosis accuracy of nonspecific mastitis as well as the ability to identify with breast cancer.Methods 31 cases of nonspecific mastitiswere examined by mammography and color Doppler's ultrasonic.All cases were punctured by biopsy or surgery, and confirmed by pathology.Results The coincidence rates between mammography and color Doppler's ultrasonic with pathology were 64.5% and 67.7% respectively.The coincidence rate between the combined mammography and color Doppler's ultrasonic with pathology were 87.1%.There was no statistically significant difference between the two separate inspection methods (P>0.05).Conclusion Mammography and color Doppler's ultrasonic have their own advantages in the diagnosis of nonspecific mastitis, and are easy to be confused with breast cancer.Combining mammography with ultrasonography can improve the accuracy of unspecific mastitis diagnosis and the ability to identify breast cancer.

Objective To analyze the clinical effect of the treatment of secretory otitis media with puncture combined with injection.MethodsThe control group was given tympanic membrane puncture combined with conventional drug treatment, and the experimental group was given combined with tympanic tympanic membrane puncture injection and conventional medicine for treatment.ResultsThe total effective rate of the experimental group (95.2%) was significantly lower than that of the control group (66.7%)(P<0.05);The incidence of complications in the experimental group (6.3%) was significantly lower than that in the control group(22.2%)(P<0.05).ConclusionClinical efficacy and safety of the treatment of secretory otitis media with puncture and injection.

Several signaling pathways are involved in clonal proliferation of lymphoma cells and disease progression. It has been shown that cyclic adenosine monophosphate (cAMP) signaling pathway, as an important intra-cellular messenger delivery system, is related to modulation of multiple physiological processes including cell prolifer-ation, secretion, metabolism and apoptosis. Manipulating the cAMP signaling pathway can induce cell cycle arrest and apoptosis of various malignant lymphoid cells, which involves mitochondria-dependent apoptosis and cAMP-modulated protein kinases as well as changes of intracellular mediators. In-depth research and analysis of cAMP-induced apoptosis in lymphoma cells would provide potential targets and possible therapeutic means for the treatment of lymphoma.

Gallstone disease is highly prevalent in clinic,particularly in women and some specific ethnic groups.The formation of water-insoluble cholesterol crystals is due to a misbalance between the three major lipids present in the bile:cholesterol,bile salts,and phospholipids.Many proteins implicated in biliary lipid secretion in the liver are regulated by several transcription factors,including nuclear receptors LXR and FXR.Human and murine genetic,pathophysiological evidence is consistent with the relevance of these nuclear receptors in gallstone formation.In addition,there is emerging data that also suggests a role for estrogen receptor ESR1 in abnormal cholesterol metabolism leading to gallstone disease.A better comprehension of the role of nuclear receptor function in gallstone formation may help doctors to design new and more effective therapeutic strategies for this highly prevalent disease condition.