Objective To investigate the effect of recombinant rat augmenter of liver regeneration (rrALR) on the biological activities of rat glomerular mesangial cell(GMC) with or without IL-1?. Methods GMC proliferation and the level of TGF-? protein were determined by 3H-TdR incorporation and ELISA. Results The stimulating activity of IL-1? to rat GMC was enhanced by rrALR with the concentrations from 0.005 pg/ml to 0. 1ng/ml. But rrALR could not promote the proliferation of GMC without IL-1?. Conclusion rrALR may be a new mediator of inflammation, which promote proliferation and TGF-? secretion in rat GMC stimulated by IL-1?.

Objective To evaluate the effects of recombinant human hematopoietic synergistic factor (rhHSF) on rapid mobilization of the peripheral blood stem cells. Methods 8 monkeys were divided into rhG-CSF 10?g/(kg?day) and rhG-CSF 10?g/(kg?day) + rhHSF 500?g/(kg?day) group, with 4 monkeys in each group. All of them received rhG-CSF subcutaneously once daily for 4 days, and in the latter group, the animals received rhHSF subcutaneously in single dose on the fifth day. Results The highest counts of CD34~+ cell, CFU-GM and neutrophils in animals receiving rhHSF only were 23, 1.8 and 3.3 times of baseline value at 180, 45 and 60 min, while those in animals receiving rhG-CSF and rhHSF were 41.3, 8.3 and 4.9 times of base line value. Conclusion rhHSF can rapidly mobilize hematopoietic stem cells and neutrophils into peripheral blood, and it is found to synergize with G-CSF to augment stem cell mobilization, suggesting a potent clinical utility of rhHSF in peripheral blood stem cells transplantation.

Objective To study the effect of recombinant rat augmenter of liver regeneration (rrALR) on proliferation and apoptosis of renal tubular cells in vitro. Methods The cultured human renal tubular cell line (HK2 cells) was divided into several groups with various concentration of gentamicin(GM) or/and rrALR. The proliferation of HK2 cell was evaluated by 3H-TdR incorporation. The apoptosis of HK2 cells was assessed by AO/EB staining and flow cytometer using Annexin V-FITC and propidium iodide (PI) staining. Results (1 ) rrALR promoted HK2 cell proliferation in a time-dependent and dose-dependent manner. The proliferative effect was significant with the concentration from 25 ng/ml to 50 ug/ml (P

Objective To investigate the trend of the national Kaschin-Beck disease condition, and provide the scientific basis for the government to make preventing strategy. Methods The data were collected from national surveillance on Kaschin-Beck disease(KBD) condition from 2000 to 2007 by restrospective method. The national X-ray detective rates and KBD condition in the eastern and western areas of China were analyzed. Results Monitoring data were collected from 189 monitoring points in 14 provinces of China, and 21 287 X-ray films were photographed of right hands of children from 2000 to 2007. X-ray detective rate was decreased significantly of national KBD from 2000 to 2007. The condition in the west of China is slightly more serious than the eastern areas, but the average of X-ray detective rate was also decreased from 21.75% to 7.30%. National condition is basically controlled. X-ray detective rate had been controlled below 5%, exept for Qinghai, Tibet and Inner Mongolia and so on. Conclusions Ninty percent of the wards have reached the controlled level according to the X diagnosis standard. In terms of the severity of the disease, most of the wards are occupied by patients with slight condition, 10% ward with moderate condition, less than 1% ward with serious condition.

Actinomyces ; isolation & purification ; Actinomycosis ; microbiology ; Apicoectomy ; methods ; Candida albicans ; isolation & purification ; Candidiasis ; microbiology ; Enterococcus faecalis ; isolation & purification ; Foreign Bodies ; complications ; Gram-Positive Bacterial Infections ; microbiology ; Humans ; Microsurgery ; methods ; Periapical Periodontitis ; etiology ; microbiology ; surgery ; therapy ; Radicular Cyst ; complications ; Root Canal Filling Materials ; therapeutic use ; Root Canal Therapy ; methods

To study the level of macrophage migration inhibitory factor (MIF) in serum and the expression of MIF mRNA in abdominal subcutaneous adipose tissue,and to investigate its impact on insulin resistance and islet β-cell dysfunction in gestational diabetes mellitus (GDM).120 pregnancy women from the Affiliated Hospital of Qingdao University Medical College and Taian Central Hospital were enrolled,including 60 GDM women and 60 women with normal glucose tolerance (NGT).The serum MIF in GDM group was higher than that of NGT group [(3.58±1.02 vs 1.23±0.62) ng/ml,P<0.01].Multiple stepwise regression analysis showed that body mass index was an independent affective factor of the serum levels of MIF (r2 =0.516).The serum levels of MIF and the expressions of MIF mRNA in abdominal subcutaneous adipose tissue were significantly higher in GDM group than NGT group.MIF may contribute to insulin resistance and β-cell dysfunction in GDM.Body mass index seems to be an independent factor in affecting the serum levels of MIF.

Objective To explore the effect of applying problem-based learning(PBL)in clin-ical anesthesiology demonstration for clinical undergraduates. Methods Eighty-nine 2009 clinical undergraduates were divided into two groups:experimental group and control group. Students in ex-perimental group received PBL while those in control group received traditional teaching. The effect was evaluated according to the results of case examination and investigation table. The results were analyzed by X2 test. Results Most students (42/45) answered questions correctly in experimental group while only 30 of 44 students(30/44)did in control group(P<0.05). Concerning the results of investigation,PBL had more advantages than traditional teaching method in the following 5 aspects:self-studying ability(86.7%,39/45),clinical thinking ability(73.3%,33/45),information seeking ability (75.6%,34/45),expressing ability (82.2%,37/45),communicating between students (75.6%, 34/45)(P<0.05). Conclusions PBL has evident advantages in anesthesiology clinical demonstration. It can inspire students,cultivate abilities,enhance critical thinking and finally improve teaching effect.

Objective To study the time distribution of non-agriculture employed population spent in a working day in China, and to provide basic information for intervention strategies.Methods The data of 2002 China Nutrition and Health Survey were used. The information on daily activities including occupation,transportation,exercise,housework,sedentary activity and sleep was described.Results Non-agriculture employed population spent 8.41 h,0.58 h,0.09 h,3.11 h,1.40 h and 7.89 h on occupation, transportation,exercise,sedentary activity,housework and sleep,respectively.Administrator,technologist and clerks spent less time on occupation activity than service workers,production and transportation workers and others did,and they spent more time on sedentary activity.Male spent more time on occupation activity and less time on housework and sleep than female did.People in rural area spent more time on occupation activity than those in urban area,but less time On transportation and sedentary activity.Conclusion Differences in time use among different employed groups,gender and area were found,which should be considered when intervention measure is developed.

AIM:To investigate the effects of recombinant rat augmenter of liver regeneration (rrALR) on apoptosis of renal tubular cells (NRK-52E cells) induced by gentamycin sulfate (GM). METHODS: The cultured NRK-52E cells were divided into four groups: normal control cells, cells with GM (GM 1.6 g/L) or GM and rrALR (15 mg/L or 25 mg/L) treatments. The apoptosis of cultured cells were assessed at 24 h, 48 h by AO/EB staining, DNA agarose gel electrophoresis analysis and flow cytometry using Annexin V-FITC and propidium iodide (PI) staining. The protein and mRNA expressions of Bcl-2 and Bax were detected by Western blotting and RT-PCR, respectively. RESULTS: (1) rrALR inhibited NRK-52E cells apoptosis induced by GM (P

BACKGROUND:Seed cells from different sources have different ability in cell adhesion,proliferation,and differentiation,which can led to bioactive diversity in constructed tissue engineered products. OBJECTIVE:To explore the differentiation ability of different original fetal osteoblasts during constructing tissue engineered periosteum at molecular level. DESIGN,TIME AND SETTING:The contrast observation was performed at the Central Laboratory of Shaanxi Provincial Peoples’ Hospital between July 2007 and July 2008. MATERIALS:The human amnion cells(consent was obtained from the puerperant) were prepared human acellular amniotic membrane(HAAM) . METHODS:Periosteum-origin osteoblasts(POB) and cranium-origin osteoblasts(COB) were seed on HAAM,cultured for 2,4,6,8,and 10 days,and then their total RNA was extracted,which were reversely transcripted to cDNA. The real-time PCR analysis was used to reveal core binding factor ?l(Cbfa1) ,Osterix,and the cycle threshold was also measured. MAIN OUTCOME MEASURES:The expression of Cbfa1,Osterix,as well as osteocalcin. RESULTS:On tissue engineered periosteum,the expression of Cbfa1 in POB was lower than it that in COB(P