Aim To study the protective effects of limb remote ischemic postconditioning ( LRIP ) on is-chemic stroke rats under hyperglycemia and explore the mechanisms. Methods Rats were given 50% glucose (6 mL·kg-1 ) by intraperitoneal injection to get acute hyperglycemia model. Then middle cerebral artery oc-clusion ( MCAO) models were created. After blocking middle artery for 1. 5 h and reperfusion for 2 h, behav-ioral testing, infarct size of brain, NO concentration and SOD activity in the serum of those rats were detec- ted. Results LRIP could improve behavioral score, decrease the area of cerebral infarction, increase the concentration of NO and the SOD activity in serum of MCAO rats. Conclusion LRIP can relieve cerebral ischemia-reperfusion injury of MCAO rats under acute hyperglycemia.

Objective: To observe the improving effect of muscle regions of meridians needling method on the upper limb function in children with cerebral palsy of spastic hemiplegic type. Methods: A total of 100 children with cerebral palsy of spastic hemiplegia type were divided into a treatment group and a control group according to the visiting sequence, with 50 cases in each group. The control group was treated with conventional rehabilitation plus conventional acupuncture treatment. The treatment group was treated with conventional rehabilitation plus muscle regions of meridians needling method. The electromyography (EMG) signal values of triceps brachii and pronator teres were detected before treatment, and 3 months and 6 months after treatment. The clinical efficacy was evaluated by Peabody developmental motor scale-fine motor (PDMS-FM) and fine motor function measure (FMFM). Results: Three and six months after treatment, the EMG signal values of triceps brachii and pronator teres, grasping scores and visual-motor integrated scores of PDMS-FM and the FMFM scores in both groups increased to varying degrees compared with the same group before treatment, and the intra-group differences were all statistically significant (all P<0.05). Six months after treatment, the results of the above three items in the treatment group were all better than those in the control group, and the differences between the groups were statistically significant (all P<0.05). Conclusion: Muscle regions of meridians needling method added on the basis of conventional rehabilitation can effectively reduce the muscle tone of upper limb and enhance the muscle strength, and improve the upper limb function in children with cerebral palsy of spastic hemiplegia type. The efficacy is superior to that of the conventional rehabilitation plus conventional acupuncture treatment.

To construct a TK-/gG- mutant of pseudorabies virus, the gG-detected transfer vector pUSKKBB and genomic DNA of pseudorabies virus TK-/gG-/LacZ+ were co-transfected into IBRS-2 cells. Transfection progeny were plated onto PK-15 cells and incubated for 2 days under methylcellulose. Then the overlay was removed and replaced by 1% low melting point agarose in DMEM supplemented with 150 microg/mL X-gal. After 2 days, white plaques were screened for and purified 4 times. By PCR amplification of gG-deleted gene and LacZ gene, a recombinant virus with TK-/gG- phenotype was confirmed. Sequence of the PCR product revealed that there were 1,176 bp detection in gG gene of the PRV TK-/gG- mutant. Amplifying the gG-deleted gene of different generations of the TK-/gG- mutant showed that the mutant was stable within PK-15 cells. TCID50 assay indicated that the recombinant virus grows well on PK-15 cells. The mice immunized with the TK-/gG- virus showed no sign of abnormality. As a control, all mice inoculated with PRV strain died from the infection. All mice that received TK-/gG- survived after a lethal PRV challenge. However none of the mice injected with phosphate-buffer saline (PBS) survived from the challenge. The above results demonstrated that the recombinant virus could be a candidate marker vaccine strain for eradicating pseudorabies in pig herds.
Animals ; Herpesvirus 1, Suid ; genetics ; pathogenicity ; Mice ; Mice, Inbred BALB C ; Mutation ; Pseudorabies Vaccines ; immunology ; Swine ; Thymidine Kinase ; genetics ; Vaccines, Synthetic ; Viral Envelope Proteins ; genetics ; immunology

OBJECTIVETo investigate the level of the serum IL-23 and its correlation with serum biochemical indices of liver function tests and HBV DNA load in patients with chronic severe hepatitis B.

METHODSFifty patients with chronic severe hepatitis B (severe hepatitis group) and 18 healthy controls (control group) were enrolled in the study. The serum IL-23 expression level was detected by ELISA method. The correlation between IL-23 and ALT, AST, TBil, HBV DNA load was analyzed using Pearson's correlation analysis, respectively.

RESULTSSerum IL-23 expression level in severe hepatitis group was significantly higher than that of control group (P < 0.05). Serum IL-23 level was positively correlated with serum ALT, AST, respectively (P < 0.05), but was not correlated with TBil and HBV DNA load, respectively (P > 0.05).

CONCLUSIONSerum IL-23 expression level was increased in patients with chronic severe hepatitis B and was associated with the severe of inflammation. We, therefore, believe that IL-23 might be involved in the pathogenesis of chronic severe hepatitis B.

Adult ; Alanine Transaminase ; blood ; Aspartate Aminotransferases ; blood ; DNA, Viral ; analysis ; Female ; Hepatitis B, Chronic ; immunology ; virology ; Humans ; Interleukin-23 ; blood ; Male ; Middle Aged

Objective To evaluate the efficacy and safety of domestic human recombinant FSH (rhFSH) in women with anovulation of WHO groupⅡ. Methods A randomized, blind, parallel-controlled, non-inferiority and multicenter study was performed. A total of 534 admitted to 13 hospitals from May 2008 to August 2009. There were 531 women with ovulatory disorder was included in the statistical analysis, were randomly divided into test group (domestic rhFSH, n=352) and control group (imported rhFSH, n=179). Percentage of cycle with mature follicle, ovulation rate, clinical pregnancy rate, multiple pregnancy rate, ovarian hyperstimulation syndrome (OHSS) and adverse events were observed. Results No statistical significant differences (P>0.05) were observed between the two groups in terms of the efficiency on mature follicle [91.8%(323/352) versus 88.8%(159/179)], ovulation rate [91.3%(295/323) verus 90.6%(144/159)], clinical pregnancy rate [19.2%(62/323) verus 18.2%(29/159)], the number of the follicles<14 mm, the level of serum LH and progesterone, the thickness of endometrium on the day of hCG administration. The number of follicle≥18 mm and 14 mm≤follicle<18 mm and the level of serum estradiol on the day of hCG in the test group were significantly higher than those in the control group (P<0.05). The number of days of rhFSH administration in the test group was significantly less than that in the control group [(9.8±2.2) versus (11.4± 0.6) days, P<0.05], the dosage of rhFSH was significantly lower than that in the control group [(879 ± 419) versus (1 043 ± 663) U, P<0.05]. The multiple pregnancy rate in the test group was significantly higher than that in the control group [21% (13/62) versu 10% (3/29), P<0.05]. The incidence of OHSS and adverse events were similar between the two groups (P>0.05), and no other adverse events were observed in test group during treatment. Conclusion Ovarian stimulation with domestic rhFSH is effective, safe and economical in women with anovulation of WHO groupⅡ.

OBJECTIVETo investigate the relationship of the serum chemokine RANTES level in patients with chronic hepatitis B among different clinical severity and to explore its possible reasons.

METHODS144 patients with chronic hepatitis B were divided into mild-moderate (46), serious (51) or severe group (47) according to the different clinical severity and 18 normal cases were taken as normal control. The serum level of chemokine RANTES was detected with an ABC-ELISA assay. Statistical analysis was performed on the software of SPSS 13.0.

RESULTSThe serum chemokine RANTES levels in patients with chronic hepatitis B (2227.06 +/- 790.80, 5878.49 +/- 3334.58, 3482.77 +/- 2315.62 ng/L in mild-moderate, serious and severe group respectively) were significantly higher than that in the normal control (329.46 +/- 152.00 ng/L). The differences between each two hepatitis groups were also statistically significant (P < 0.05) and serum chemokine RANTES level in serious group was highest among them.

CONCLUSIONSerum chemokine RANTES level in patients with chronic hepatitis B elevates significantly and it might play an important role in the pathogenesis of chronic hepatitis B.

Adolescent ; Adult ; Aged ; Case-Control Studies ; Chemokine CCL5 ; blood ; Female ; Hepatitis B, Chronic ; blood ; immunology ; Humans ; Male ; Middle Aged ; Young Adult

OBJECTIVESTo screen polymorphisms in the upstream region of S100A8 gene and to detect whether the polymorphisms were associated with aggressive periodontitis.

METHODSThirty aggressive periodontitis patients and twenty-eight healthy controls were recruited for the study with informed consent. All subjects were of Chinese descent and systemically healthy. The regions about 800 bp upstream from the ATG start codon in exon 2 of the S100A8 gene of 10 patients and 8 controls were amplified by polymerase chain reaction (PCR) and analyzed by direct sequencing. A single nucleotide polymorphism (SNP) at 94 bp upstream from the ATG start codon was selected, and then the shorter regions (about 250 bp upstream from the ATG start codon) of the rest subjects were also amplified by PCR and analyzed by direct sequencing. The frequency of the SNP and the distribution of the genotype were detected and compared between the two groups.

RESULTSA nucleotide substitution (A-->G) at 94 bp upstream from the ATG start codon was demonstrated in Chinese, which was in a cis-acting element, named gamma interferon response element (gamma-IRE) in intron 1 of S100A8 gene. All of the subjects that carried the polymorphism were heterozygous. There was no statistically significant difference in the frequency of allele 2 (corresponding to the nucleotide G) between patients and controls (11.7% vs. 17.9%, chi2 = 0.887, P > 0.05). The prevalence of the heterozygous genotype was 23.2% and 35.7% (chi2 = 1.07, P > 0.05) in patients and controls, respectively.

CONCLUSIONSThis is the first report that a nucleotide substitution of S100A8 gene was demonstrated in Chinese. The frequencies of allele 2 and heterozygous genotype were lower in patients, but there is no statistically significant difference between the aggressive periodontitis patients and healthy controls in this preliminary study.

Aggressive Periodontitis ; genetics ; Alleles ; Calgranulin A ; genetics ; Female ; Gene Frequency ; Genetic Predisposition to Disease ; Humans ; Male ; Periodontitis ; genetics ; Point Mutation ; Polymorphism, Genetic ; Polymorphism, Single Nucleotide ; genetics ; Promoter Regions, Genetic ; genetics

OBJECTIVETo investigate the clinical features and the short-term efficacy of ETV treatment of 132 HBeAg negative and 51 HBeAg the HBeAg negative acute-on-chronic hepatitis B liver failure.

METHODSpositive patients with acute-on-chronic liver failure were rolled. 84 patients were treated with ETV (0.5 mg daily) and routine supportive therapy. 99 patients in the control group received supportive therapy only. The clinical features and the short-term (180 days) efficacy of ETV were compared between the HBeAg negative and HBeAg positive groups.

RESULTSThe age of HBeAg negative patients was significantly higher (P = 0.001). The viral load was significantly higher in the HBeAg positive group (P = 0.001). There was no significant difference in the severity and the mortality in control group between the different HBeAg groups. In the antiviral treatment groups, 20/25 HBeAg positive patients survived and 32/59 HBeAg negative patients survived (P = 0.004). The mortality of HBeAg negative patients in control group was higher than that in treatment group (P = 0.032). In HBeAg negative patients with the HBV DNA <5 log copies/ml and HBV DNA >3 log copies/ml, 10/18 patients in treatment group survived and 6/30 patients in control group survived (P = 0.011).

CONCLUSIONSThere was no significant difference in the mortality between different HBeAg patients in routine treatment group. Antiviral therapy with ETV significantly improve survival rate in HBeAg negative patients. In patients with the HBeAg negative and HBV DNA <5 log copies/ml and >3 log copies/ml, antiviral therapy with ETV can improve the survival rate.

Adolescent ; Adult ; Aged ; Antiviral Agents ; therapeutic use ; Female ; Guanine ; analogs & derivatives ; therapeutic use ; Hepatitis B e Antigens ; blood ; metabolism ; Hepatitis B virus ; drug effects ; physiology ; Hepatitis B, Chronic ; blood ; drug therapy ; mortality ; Humans ; Male ; Middle Aged ; Treatment Failure ; Young Adult

OBJECTIVETo establish the BGC-823/WTX-EGFP gastric cancer cell line with stable expression of Wilms tumor gene on the X chromosome (MTX) for functional analysis of WTX gene.

METHODSThe full-length WTX cDNA was amplified from human embryonic kidney 293FT cells and cloned into the pEGFP-N1 vector containing the reporter gene of green fluorescence protein. The recombinant pEGFP-WTX expression vector, after digestion by restriction enzyme to identify the size of target gene fragment, was transfected into 293FT cells and the expression of fluorescent reporter gene was observed under fluorescence microscope. pEGFP-WTX vector was transfected into human gastric cancer BGC-823 cell line to establish BGC-823/WTX-EGFP cell line stably expressing WTX. Quantitative RT-PCR and immunocytochemical staining were used to detect the expression of WTX in both BGC-823/WTX-EGFP and control BGC-823 cells.

RESULTSThe recombinant pEGFP-WTX plasmid was successfully constructed and verified by PCR and sequencing. The mRNA and protein expressions of MTX were significantly increased in BGC-823/WTX-EGFP cells as compared with those in the control cells.

CONCLUSIONThe full-length WTX expression vector (pEGFP-WTX) and BGC-823/WTX-EGFP gastric cancer cell line have been successfully established to facilitate further functional study of WTX gene.

Cell Line ; Cell Line, Tumor ; Chromosomes, Human, X ; genetics ; DNA Restriction Enzymes ; DNA, Complementary ; Genes, Wilms Tumor ; Genetic Vectors ; Green Fluorescent Proteins ; genetics ; Humans ; Plasmids ; Stomach Neoplasms ; genetics ; Transfection