Objective Experimentally to investigate the changes in vasospastic canine basilar arteries. Methods Vasospasm of basilar arteries was induced by double-injecting autologous blood into the brain cisterns. 28 adult mongrel dogs of both genders served as the subjects, of them 20 involved in experimental group and accepted the PTA treatment and the other 8 doges were in negative control group without any treatment. PTA was performed by a single inflation of balloon for 10 seconds at a peak pressure of 2.0 atmospheres. Angiography was re-performed at 30 day, 90 day and 300 day following PTA, respectively. The histologic changes were then evaluated using light and electron microscopy. Results Caliber of basilar arteries of all canines decompressed obviously, from 1.3 mm to 0.8 mm. In test group, PTA succeeded in 15 ones, with caliber of vasospastic basilar arteries enlarged obviously after PTA ( P

ATP-Binding Cassette Transporters ; immunology ; metabolism ; Animals ; Herpes Simplex ; immunology ; virology ; Herpesvirus 1, Human ; immunology ; metabolism ; Humans ; Immediate-Early Proteins ; immunology ; metabolism ; Viral Proteins ; immunology ; metabolism

This study aimed to investigate viral infections and the prevalence of influenza-like illness (ILI) in Shijiazhuang, China, in 2011 and to provide a scientific basis for the diagnosis and control of respiratory tract infections. Throat swab specimens were collected from 483 cases of ILI who were outpatients in the influenza surveillance sentinel hospitals in Shijiazhuang between January and December 2011. All specimens were examined by multiplex RT-PCR for the following 15 respiratory tract viruses: adenovirus (ADV), human rhinovirus (HRV), human parainfluenza virus (PIV types 1-4), influenza virus A (FluA), influenza virus B (FluB), human enterovirus (HEV), respiratory syncytial virus (RSV-A and -B), human metapneumovirus (HMPV), human coronavirus (HCoV-229E/NL63 and -OC43/HKU1), and human bocavirus (HBoV). Among the 483 cases of ILI, 214 (44.31%) were positive for viruses, including ADV (8.7%), HEV (8.7%), RSV-A (8.07%), HRV (7.45%), FluA (5.38%), HCoV-OC43/ HKU1 (2.9%), PIV-3 (2.9%), HMPV (1.86%), PIV-1 (1.24%), HCoV-229E/NL63 (1.04%), PIV-2 (1.04%), HBoV (0.83%), and FluB (0.41%). Twenty-six (5.38%) of all cases were co-infected with two or more viruses, most commonly HEV/HRV with other viruses. Cases of viral infection were detected throughout the year, with peaks in January and February. ADV and HRV were detected throughout almost the whole year without obvious seasonality. HEV was detected between April and November, with a peak of prevalence in summer and autumn. FluA and FluB reached epidemic levels mainly in winter and spring. All cases of RSV were identified to be subtype A. PIV infection was mainly caused by PIV-3. The positive rate of HCoV-OC43/HKU1 infection was significantly higher than that of HCoV-229E/NL63. The leading five viruses that resulted in ILI Shijiazhuang in 2011 were HEV, ADV, RSV-A, HRV, and FluA, and these viruses have different epidemiological features.
Adolescent ; Adult ; Aged ; Child ; Child, Preschool ; China ; epidemiology ; Female ; Humans ; Infant ; Influenza, Human ; epidemiology ; virology ; Male ; Middle Aged ; Respiratory Tract Infections ; epidemiology ; virology ; Virus Diseases ; epidemiology ; virology ; Viruses ; classification ; genetics ; isolation & purification ; Young Adult

Objective To investigate the viral etiology and the epidemilogy of acute upper respiratory tract infection in Hebei Province from 2013 to 2015,providing scientific basis for diagnosis,prevention and control of the respiratory tract infection.Methods l 551 throat swab samples of the acute upper respiratory infection patients from four hospitals in Hebei Province were collected,Multiplex real-time PCR was used to detect 15 kinds of respiratory tract viruses for all specimens after nucleic acids extraction.Results Totally 714 positive samples were obtained from 1 551 samples,with a positive rate of 46.03％.Human rhinovirus(186,11.99％)was detected as the most common viral species,followed by human parainfluenza 3(167,10.77％),respiratory syncytial virus (122,7.87％),adenovirus (108,6.96％),influenza B virus (56,3.61％),human metapneumovirus(40,2.58％),influenza A virus(39,2.51％),human boca virus (38,2.45％),human parainfluenza 1 (35,2.26％),human coronavirus-229E/NL63 (33,2.13％),human enterovirus (32,2.06％),human parainfluenza 4(31,2.00％),human coronavirus-OC43 (30,1.93％),and human parainfluenza 2(11,0.71％).176 cases (11.35％) were detected as co-infection.The infection rate(56.07％) of under 5 years old group was higher than that of other groups.Conclusions The predominant respiratory viruses,who are responsible for acute upper respiratory tract infections in Hebei Province,are HRV,PIV3,RSV,ADV and IFV.Each virus possess the specific epidemiological feature.

The vascular architecture of the adult man testis was examined by using thevascular casting method combined with scanning electron microscopy.The circuitousmain branches of testicular artery ran radially from testicular porta towards testicu-lar free border in tunica vasculosa.The test(?)cular surface veins were accompaniedby the main branches of testicular artery and they converged from testicular circum-ference circuitously towards testicular porta.There were centripetal artery andvein as well as centrifugal artery and vein in the septula testis.The intertubularblood vessels and the peritubular capillary plexus were contained in the testicularlobules.The latter was made up of two capillary layers.The outer layer was capil-lary network interwined in fine capillaries.The inner layer was coarse capillarycircles.The interstitial capillary plexus was situated in interstitial tissue between theseminiferous tubules and compose of capillaries arising from the origin of someperitubular capillaries.

Objective; To evaluate the influence of electrolyte's concentration at micro-arc oxide treatment on the bonding strength of titanium to porcelain. Methods; CP titanium specimens with size of 25 mm ×3 mm ×0. 5 mm were treated with micro-arc oxidation (MAO) in Na_2SiO_3 solution of three different concentrations. After ultra-low-fusing porcelain was applied, a three-point-flexure-test was used to evaluate the bonding strength of titanium to porcelain. The surface of the specimens was observed by SEM and EDS, as well as the interface between titanium and porcelain. Results; The bonding strength values between titanium treated with MAO and porcelain was significantly higher then the control groups, Croup 20 g/L has the highest bonding strength values. SEM/EDS suggested that a porous thin layer of oxide which contains Si element is created by MAO, and higher concentration of Na_2SiO_3 leads to more Si element in oxide. Conclusion; MAO treating can improve the bonding strength between titanium and ceramic. Electrolyte's concentration has an effect on the bond strength between titanium and porcelain.

Objective To investigate the clinical value of tissue polypeptide specific antigen(TPS),neuron-specific enolase(NSE),carcinoembryonie antigen(CEA)and CA125 in serum of small cell lung cancer(SCLC)patients and its significance in diagnosis and disease monitoring.Methods Serum leveh of TPS was detected using ELISA and serum levels of NSE,CA125 and CEA was detected using ECLin 27 1 SCLC patients.80 pulmonary benign disease patients and 224 normal healthy people.Diagnostic values of these tumor markers were analyzed by receiver operative characteristic(ROC)curve.Results The levels of TPS,NSE,CA125 and CEA iu the serum of SCLC group were signifieanfly higher than those in pulmonary benign disease and healthy group(Z＞1.90,P＜0.01).The levels of TPS and NSE in the serum of extensive stage small cell lung cancer(ESCLC)patients were significantly higher than those in limited stage small cell lung cancer(LSCLC)(Z=2.69,2.27,P=0.009,0.02 respectively).,The level of TPS and NSE showed statistical significance among SCLC patients with different prognosis after therapy(Z=4.06,3.11.P=0.001,0.007 respectively).The TPS+NSE showed the highest sensitivity of 86.7%,and the specificity,PPV and NPV were 75.0%,81.0% and 82.2%,respectively.Conclusions Serum levels of TPS,NSE,CA125 and CEA are useful for SCLC diagnosis.TPS+NSE shows the highest clinical values in SCLC diagnosis and prognosis.

Objective: To study the basic information of life way disease and the corresponding risk factors of behavior in the community of Peking University Health Science Center, understand the background issue and social support system, and analyze the requirement for community health service so that the critical issue for health can be dealt with and the comprehensive program of prevention and treatment of the disease can be accordingly supplied. Methods: The random face to face questionnaire about life way disease and risk factors in 1 051 residents over 6 years of age in the community of Peking University Health Science Center was conducted according to the typical sampling principle in 1 primary school, 1 middle school, 1 university and its community, and the investigations of health data from the hospital and the police office in the community were also conducted through discussing about life ways risk factors. Accordingly , the data obtained by way of EXCEL were analyzed and processed using SPSS 11.5. Results: Diagnostic data of demography, epidemiology, behavior environment, education and organization were obtained concerning the public health of the Peking University Health Science Center community. Conclusion: The key health issue in the Peking University Health Science Center community is significantly influenced by the bad way of life. And some behavior risk factors, such as fatness, lack of outdoor exercise in the community members. Thus, the major necessary health service of Peking University Health Science Center residents is to carry out a comprehensive management program of life way disease for the whole people.

BACKGROUND:Elderly patients have more loose bone. After fractures, bones are mostly crushed. Simultaneously, many elderly patients experience many internal diseases. Various organs have dysfunction. Surgery tolerance is poor, which also brings difficulty in treatment with internal fixator, and increases the risk of therapy. Thus, the choice of internal fixation method is not uniform. OBJECTIVE: To compare and analyze the repair effects of proximal femoral nail anti-rotation blade and anatomic proximal femoral locking plate in treatment of unstable intertrochanteric fractures in the elderly. METHODS:A total of 100 elderly patients with unstable intertrochanteric fractures were selected from the Youyi Branch, Kunshan First People’s Hospital from April 2011 to June 2013. They were randomly assigned to two groups. Patients in the experimental group received internal fixation with proximal femoral nail anti-rotation blade. Patients in the control group received anatomic proximal femoral locking plate. After treatment, folow-up was conducted. Operative indexes, postoperative Harris score for hip function and incidence of complications were compared and analyzed in both groups. RESULTS AND CONCLUSION: No significant difference in various indexes was detected in both groups (P > 0.05). Harris scores were significantly better in the experimental group than in the control group (P < 0.05). Postoperative complications were significantly less in the experimental group than in the control group (P < 0.05). These results indicated that internal fixation of proximal femoral nail anti-rotation blade for unstable intertrochanteric fractures in the elderly achieved satisfactory results in the recovery of joint function and had the advantage of few complications.

Coxsackievirus Infections ; immunology ; virology ; Enterovirus A, Human ; physiology ; Enterovirus Infections ; immunology ; virology ; Gene Expression Regulation ; immunology ; Gene Expression Regulation, Viral ; Host-Pathogen Interactions ; immunology ; Humans