2.Balloon dilatation of intrahepatic biliary strictures in liver transplantation
Guang CHEN ; Kai CHEN ; Xiuying LIU ; Ji QI
Chinese Journal of Radiology 2001;0(02):-
Objective To evaluate the efficacy of balloon dilatation in the treatment of intrahepatic biliary strictures in patients with liver transplantation. Methods Of the 100 patients with liver transplantation, 16 patients had intrahepatic biliary strictures and received balloon dilatation treatment. Results Initial technical balloon dilatation was successful in 14 cases but failed in 2 cases. There were no procedure-related complications. 4 restenosis occurred and they were treated with repeated balloon dilatation treatment. Conclusion Balloon dilatation represented an effective and relatively safe treatment for biliary stricture in liver transplant recipients. For restenosis, balloon dilatation was also an effective treatment.
3.Association between maternal blood lipid level during pregnancy and risk of small-for-gestational-age infants
CHEN Hui Qi ; LUO Qiong ; CHEN Guang Di
Journal of Preventive Medicine 2021;33(1):41-45
Small for gestational age ( SGA ),one of the major adverse pregnancy outcomes, significantly increases the risk of perinatal death and metabolic diseases in adulthood. It is of great significance to strengthen early surveillance and intervention for SGA prevention. Dyslipidemia during pregnancy, as a common metabolic disorder, has been considered to correlate with the increased risk of SGA; however, the epidemiological evidence is still controversial. We have systematically reviewed the recent studies related to the association between serum lipid level during pregnancy and the risk of SGA, so as to provide reference for prevention and intervention of SGA.
4.Design and Quality Evaluation of Dihydromyricetin in Self-emulsifying Drug Delivery System
Na QI ; Guang LIU ; Chunyan LIU ; Xu CHEN
Herald of Medicine 2014;(7):940-944
Objective To design and optimize the self-emulsifying drug delivery system ( SEDDS) of dihydromyricetin, and evaluate its quality in vitro. Methods The solubility of dihydromyricetin was measured in different oil phases,emulsifier and co-emulsifier solution. The best prescription was selected by comprehensively evaluating emulsification speed,particle size and drug loading via using the ternary phase diagram and orthogonal design test. Results The optimized formula of the self-emulsifying drug delivery system was oleic acidTween-80span-80N-butanol =1514714. Conclusion The optimized dihydromyricetin self-emulsifying drug delivery system significantly improves the solubility of dihydromyricetin, increases the physical and chemical properties of the formulation,and raises the dissolution of dihydromyricetin in artificial gastric fluid.
5.Application and development of kinematical alighment during total knee arthroplasty.
Zhang GUO-DONG ; Chen YANG ; Guang YANG ; Xin QI
China Journal of Orthopaedics and Traumatology 2015;28(12):1162-1165
Kinematical alignment during total knee arthroplasty is an emerging process, and draws more and more attentions from scholars. Knee joint is close to normal joint after TKA through kinematical alighment, which has good clinical results and functional scores, and not increase failure probility. Thus, it may increase joint stress of patella-femur joint, lead to patellar maltracking and increase abrasion. The paper summarized defination and basical principle, operative method, clinical outcomes and deficiency of kinematical alignment during total knee arthroplasty, in order to choose a better way for kinematical alignment during total knee arthroplasty.
Arthroplasty, Replacement, Knee
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methods
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Biomechanical Phenomena
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Humans
6.Ischemic-type biliary lesions without hepatic artery occlusion after liver transplantation: early diagnosis of cholangiography
Guang CHEN ; Yingxiu LIU ; Jian WANG ; Ji QI
Chinese Journal of Radiology 2008;42(8):835-839
Objective To evaluate the value of cholangiography for early diagnosis of ischemic-type biliary lasions(ITBL) after liver transplantation. Methods Two hundred and fifty-three patients with liver transplantaion between Jan 2004 and Oct 2006 were recruited. Initial cholangiography was compared with terminal cholangiography to evaluate the value of initial cholangiography of ITBL. The t test, Chi-square test, sum rank test were used for statistics. Results Based on initial cholangiography, 189 patients were diagnosed with normal appearance, while 64 patients were diagnosed with abnormal appearance. The abnormal initial cholangiography appearances included poor filling in 33 patients and irregularity in 31 patients. Based on terminal cholangiogruphy, 199 patients were diagnosed with normal appearance and 54 patients with ITBL In patients with abnormal initial cholangiography, ITBL was occurred in 39 of 64 patients including 10 of 33 poor frlling patients and 29 of 31 irregularity patients. In patients with normal initial appearance, ITBL was only occurred in 15 of 189 patients. The abnormal initial cholangiography was associated with ITBL significantly (X2 = 79.999, P = 0.000, r = 0.490). Initial cholangiography had an overall sensitivity of 72.22%, and specificity of 87.44%, with positive and negative predictive values of 60.94% and 92.06%, respectively. The abnormal initial cholangiogruphy was a risk factor of ITBL by logistic regression analyses(OR=15.193, P=0.000). Conclusion The abnormal initial cholangiography is associated with ITBL Initial cholangiography, especially minimal irregular of intrabepatic biliary tract, is a sensitive and specific method for the detection of ITBL after liver transplantation.
8.Hepatic VX2 tumor after portal vein occlusion in rabbits:evaluation with DSA
Yue-Yong QI ; Li-Guang ZOU ; Shu-Hua DAI ; Xiao-Bing HUANG ; Ke-Qiang HAN ; Qi-Chuan ZHANG ; Lin CHEN ;
Journal of Interventional Radiology 2006;0(11):-
Objective To study the value of DSA for hepatic vascular anatomy,and to evaluate the efficacy of portal vein occlusion in rabbits with hepatic VX2 tumor.Methods Twenty New Zealand white rabbits were randomly divided into two groups with 10 in each group,including test group A and positive control group B of ham operation.For the test group A,portal branch ligation(PBL)was performed for the left external branch after 3 weeks of the tumor implantation to the left external lobe.Two weeks later,the DSA of hepatic artery and portal vein were performed in all of the rabbits.Results The total displaying effectiveness of the branches of hepatic artery by DSA was better than that by vascular perfusion.There was hypovascular blood supply to hepatic artery implantation of the tumor in the test group A,comparing with that of the group B.Conclusion DSA can clearly display spacial details of the hepatic vascular anatomy in rabbits,and play an important role in post-procedual evaluation of the portal vein occlusion in rabbits.
9.Phenolic glycosides from the stems and twigs of Strychnos cathayensis and their biological activities
Qi-ming PAN ; Shuang-gang MA ; Yong LI ; Dan ZHANG ; Hua SUN ; Qi HOU ; Xiao-guang CHEN ; Shi-shan YU
Acta Pharmaceutica Sinica 2023;57(1):170-179
Fourteen compounds were isolated from the
10.Development of a novel screening assay for inhibitors targeting HIF-1alpha and P300 interaction.
Fang-Fang LAI ; Fei NIU ; Han-Ze YANG ; Wan-Qi ZHOU ; Xiao-Guang CHEN
Acta Pharmaceutica Sinica 2014;49(6):849-853
Hypoxia is a general characteristic of most solid malignancies and intimately related to cancer progression. Homeostatic response to hypoxia is primarily mediated by hypoxia inducible factor-1alpha (HIF-1alpha) that elicits transcriptional activity through recruitment P300 coactivator. Targeting the interaction of HIF- alpha and P300 would thus constitute a novel approach for cancer treatment by suppressing tumor angiogenesis and metastasis. Here, a screening assay was developed for inhibitors targeting the interaction between HIF-1alpha and P300. The nucleotide sequence of human HIF-1alpha and P300 were cloned into pBIND and pACT vectors, named pBIND-HIF1alpha and pACT-P300. The interaction of HIF-1alpha and P300 was identified in HEK293 cell using mammalian two-hybrid system. And compound chetomin decreased their interaction in this mammalian two-hybrid system. We further verified HIF-1 inhibition effect of chetomin in U251-HRE cells. Therefore, we established a screening assay combined HIF-1alpha and P300 mammalian two-hybrid system and U251-HRE reporter assay for HIF-1 selective inhibitors.
Cell Hypoxia
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Disulfides
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pharmacology
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Drug Screening Assays, Antitumor
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E1A-Associated p300 Protein
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antagonists & inhibitors
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HEK293 Cells
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Humans
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Hypoxia-Inducible Factor 1, alpha Subunit
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antagonists & inhibitors
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Indole Alkaloids
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pharmacology
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Two-Hybrid System Techniques