Objective To analyze the therapeutic values of valsartan combined with benazepril in the treatment of patients with congestive heart failure.Methods 102 cases of patients with congestive heart failure in Nine 0 Third Hospital were enrolled in the study.They were randomly divided into the control group and the observation group, with 51 cases in each group.Both groups were given cardiac diuretic, anti-infection and other basic treatment and the control group was given benazepril on the basis of basic treatment while the observation group was additionally given valsartan on the basis of treatment in the control group.The curative effects were compared and the changes of cardiac function indexes were detected before and after treatment between the two groups.Results The effective rate of treatment was 80.39% in the control group, which was lower than that in the observation group with 96.08% (P<0.05).After treatment, the observation group LVEF increased significantly, LVESd and LVEDd decreased significantly, and each index was significantly better than that of the control group (P<0.05).Conclusion Valsartan combined with benazepril in the treatment of congestive heart failure can effectively relieve the clinical symptoms and improve the cardiac function, therefore it is worthy of clinical promotion.

By means of correlative analysis of critical care air transportation management between China Air Force and United State Air Force,five areas of importance are discussed in this paper,such as the management model of strategies for air transportation,critical care team personnel composition,professional training mechanism,normalization and standardi-zation of operation process,and intensive application of new technology and equipment.

Objective To explore the protective effect of monosialoganglioside (GM1) on rats with acute brain trauma and its relevant mechanism.Methods Localized brain contusion model in rats were constructed by Feeney's method.65 SD rats were randomly divided into three groups: sham-operation group (n=5), brain injury group (n=30) and GM1 group (n=30).The rats were killed at 3, 7, 14, 28, 56, 168 h after administration, 5 rats in each group (1 rats in sham-operation group).Bax and Bcl-2 protein expression and PARP were decected by immunohistochemical method.The neuronal apotosis was detected by TUNEL.Results There were significant differences in expression of Bax and Bcl-2 protein between brain injury group and sham-operation group at each time point (P<0.05).There were significant differences in expression of Bax and Bcl-2 protein between GM1 group and brain injury group at each time point ( P <0.05 ) , while there were no significant differences in expression of Bax and Bcl-2 protein after 14 h between GM1 group and sham-operation group.After administration, the Bax/Bcl-2 values decreased and was obvious at 14 h.Degradation of PARP and rate of neuronal apotosis in brain injury group at each time point were significantly higher than those in sham-operation group (P<0.05).Degradation of PARP in GM1 group at 28 h, 56 h, 168 h were significant lower than those in brain injury group (P<0.05), and rate of neuronal apotosis was lower at each time point than those in brain injury group (P<0.05).Conclusion GM1 could reduce value of Bax/Bcl-2, degradation of PARP and apoptosis in rats with traumatic injury brain.

Audiometry, Speech ; Humans ; Language

Objective To observe the effect of rapid reduction of physical activity on bone mass (BMC), bone mineral density (BMD)and bone metabolism in growing rats. Methods 48 male SD rats were divided into the high-intensity exercise group (T1, n=12), high tolow-intensity exercise group (T2, n=12), low-intensity exercise group (T3, n=12) and control group (Con, n=12). During the first 6 weeks,the rats in T1 and T2 group ran on treadmill with high intensity, while rats in T3 group took low-intensity treadmill exercise. 6 rats were sacrificedfrom each group at the end of 6th week. BMC, BMD of femur were measured with DEXA. Alkaline phosphatase (ALP), osteocalcin(BGP), tartrate-resistant acid phosphatase (TRACP) were detected with ELISA. The exercise intensity of T2 group was reduced rapidly to alow level as in T3 group, but remained in the other 2 groups. At the end of the second 6 weeks, the other rats were investigated again. ResultsCompared with the first measurements, ALP and BGP of the T2 group decreased significantly (P<0.05), and TRACP increased (P<0.05). There was no significant difference in BMC and BMD of femur (P>0.05). Conclusion Rapid reduction of physical activity can suppressbone formation and stimulate bone resorption of long bone among growing rats.

Objective To investigate the effect of PNS on malignant melanoma and the expression of connexin32 in melanoma. Methods The spontaneous and experimental lung metastasis models of B16 melanoma were used to investigate the inhibitory effect of PNS on tumor growth and metastasis. The expression of connexin in melanoma were detected by immunohistoehemistry. Results (1) PNS can obviously inhibit the growth of B16 melanoma. The inhibition rate of the 480mg/kg PNS group was 50. 85%. (2) PNS can obviously inhibit the lung metastasis of B16 melanoma. The number of tumor colonies in lung of the 240 mg/kg PNS group and 480 mg/kg PNS group were lower than that in the negative control group. ( 3 ) The expression of connexin32 in melanoma was detected by immunohistochemistry:PNS could up-regulate the expression of connexin32 in membrane of melanoma. Conclusion PNS can inhibit malignant melanoma growth and metastasis and could also up-regulate the expression of connexin32 in membrane of melanoma.

An electrogenerated chemiluminescence ( ECL) sensor was fabricated by employing ruthenium complex polymer as ECL signal and ionic liquid as binding reagent and a sensitive ECL method for the determination of heroin was developed. The ECL sensor was prepared by thoroughly mixing ruthenium complex polymer,graphite powder and ionic liquid,which shows good electrochemical and ECL behaviors. The detection limit for tripropylamine (TPA) was one order of magnitude lower than that observed at the paraffin modified carbon paste electrode. ECL intensity of the fabricated sensor was greatly enhanced in the presence of heroin and a sensitive ECL method was proposed for the determination of heroin based on the enhancement ECL. The ECL intensity was linear with the concentration of heroin in the range from 2. 0 ×10~(-9) mol/L to 2. 0×10~(-5) mol/L and the detection limit was 8×10~(-10) mol/L. The ECL sensor exhibited a long-term stability,fine reproducibility with relative standard deviation less than 5% for 5. 0×10~(-9) mol/L heroin in 60 continuous determinations. The developed method allows the detection of heroin in a serum sample with recovery in 94% -101%.

AIM:To explore the regulation of lumbar physiological curvature by angulation traction and longitudinal rotation and the clinical significance of repairing spinal balance on lumbar disc herniation(LDH). METHODS:①Totally 71 patients with LDH diagnosed by CT at the 451 Hospital of Chinese PLA were enrolled from October 2005 to January 2006. Three-dimensional swirl angle program control restitution bed was applied. This bed was composed of fixing chest swirl section,magnetic heat lumbar flexure massage section and traction haunch angulation section with the pull strength of 20-90 kg. ②The patients were divided into experimental group(n =38) and control group(n =33). ③All patients of the two groups received supine genuflex traction for 20 minutes and combined with longitudinal rotation for 10 minutes. Patients in the experimental group received 20? traction from below lumbar to bed,while patients in the control group received plane traction combined with longitudinal rotation. ④Lumbar function was determined by criterion of the lumbar disease made by Japanese Shaping Association to observe improved indexes and times of treatment. RESULTS:Totally 71 patients were involved in the result analysis. No significant difference was found in preoperative lumbar function between experimental group and control group(13.684?1.662,13.485?3.001,P

Objective To establish an HPLC method to determine the content of diosgenin in Paridis Rhizoma; To examine contents of diosgenin in different varieties of Paridis Rhizoma medicinal plants and from different habitats. Methods Shimadzu Inertsil ODS-C18 guard column (150 mm×4.6 mm, 5μm) was used in HPLC system; elution conditions were the mobile phase using 92% acetonitrile and 8% water (isocraticelution) at a flow rate of 1.0 mL/min with 203 nm detection wavelength. The temperature of the column oven was maintained at 30℃.Results Different varieties of Paridis Rhizoma medicinal plants and Paridis Rhizoma medicinal plants from different habitats contained diosgenin. TheParis polyphylla var.pseudothibetica in Yubei district had the highest content (6.813 7 mg/g);Paris polyphylla var.stenophylla in Chengkou County had the middle content (5.758 4 mg/g);Paris yunnanensisFranch in Shizhu county showed the lowest content (1.952 2 mg/g). The results showed that the obvious difference of chemical quality between various cultivated varieties of Paridis Rhizoma medicinal plants, which might be related with origin, variety and the cultivation technology, and so on.Conclusion The diosgenin contents ofParis polyphylla var. pseudothibetica andParis polyphylla var.stenophylla were higher than that of varieties recorded in Chinese Pharmacopeia.Paris polyphylla var.pseudothibetica andParis polyphylla var.stenophylla had important medical value.

Objective To explore the clinical significance and safety in hemihepatectomy for hepatoma without inflow occlusion.Methods A total of 62 patients with liver cancer were divided into 2 groups.The inflow was occluded in group A (n = 28) and not occluded in group B (n = 34) during hepatectomy.Subsequently,the influence of the two approaches on the parameters including intraoperative blood loss,postoperative hepatic function and complications incidence rate was comparatively analyzed.Results Hepatectomy was successfully performed in the two groups.There was no significant difference between the two groups in intraoperative blood loss (P ＞ 0.05).Significant difference existed between the two groups in postoperative hepatic function and complications incidence rate (P ＜ 0.05).Conclusions The hemihepatectomy for hepatoma without inflow occlusion can reduce complications incidence rate and profit the recovery of postoperative hepatic function parameters.