In order to explore the cellular mechanism of antishock effect of anisodamine (654-2), the changes of intramitochondrial magnesium during late hemorrhagic shock were observed. Results showed that there exists a negative correlation between the blood plasma magnesium concentration and arterial blood pressure in shocked animals and at the same time the magneium content of liver mitochondria reduced significantly; 654-2 prevented the decrease of mitochondria magnesium and the increase of blood plasma magnesium, and improved the prognosis of the shocked animals. These results indicate that 654-2 may protect the liver mitochondria of shocked animals at a cellular and subcellular levels.

Tension-free hernioptasty is a major method for treating inguinal hernia, characterizing by less wound, tension-free incision, sample processing, less dissection and separation, mild pain, rapid recovery, and short length of stay. In particular, transabdominal preperitoneal inguinal hernioplasty is considered as the most ideal method at physiologicoanatomical level. However, the effect of patch materials on postoperative recovery is generally ignored for a long time. Recent studies demonstrate that patch weight and mesh diameter are important factors for abdominal wall repairing and reconstruction. Polypropylene patch, characterizing by strong stability, high intensity, great unreactiveness, and sample processing, has become a major material for hernioptasty. However, there are still some problems of clinical application, including that polypropylene patch may induce intensive foreign body reaction and chronic inflammation as well as compliance decreasing of abdominal wall. Therefore, polypropylene patch should be chosen according to patient requirement, i.e., light, soft, and wide-aperture patch, so as to reduce onset of complication. Nerve and ligament tissues could be clearly observed from light and soft patch, and this might and prevent hurt by mistake, improve repairing operability, enhance correct putting of patch, and decrease incidence of pain. Furthermore, the structure was beneficial for in-growth of fiber tissue, longitudinal and transversal movement induced by body position alternation and muscle contraction, and reduction of discomfortableness.

The most important characteristic of optical coherence tomography (OCT)is high resolution (about 10~15μm),so it can perform high resolution cross section tomography on internal microstructure of biological tissue. From 2000 OCT first used to detect coronary artery lesions,it already gradually popularized in diagnosis and treat-ment field of coronary artery intervention,It has become a main method for intravascular detection,diagnosis and guiding treatment of coronary diseases.

With the development of modem tumor diagnostic techniques and therapies, the survival rate of malignant tumor has been remarkably increased. With the increase in survival rate of cancer-beating patients, the chance of tumor metastasis is elevated. Minimally invasive treatment provides a new strategy to the therapy of spinal metastatic carcinoma. Its benefits include smaller surgical wound,, higher efficacy and fewer complications, and thus have a broad application prospects in the field of spinal metastatic tumors. In this article, a general re-view is given on minimally invasive treatments of spinal metastatic carcinoma, as well as the latest progress in the field. It was proposed that the united application of the treatment might be a promising hot spot of tumor therapy.

Potential toxicities of three classes of dyes and pigments were introduced in this review. Some azo/benzidine dyes, anthraquinone dyes and triarylmethane dyes have carcinogenicity,some azo/benzidine dyes and anthraquinone dyes may cause skin irritation, some noncarcinogenic triarylmethane dyes have risk of serious damage to eyes and may exert long term adverse effects on the aquatic environment.

In this study, the influence of the duration of opening vestibular window and the application of suction in auditory bulla on cochlear function and ultrastructures were observed by ECochG and scanning and transmission election microscopy in 44 guinea pigs divided into six groups. The results were as follows: (1) The CAP thresholds progressively enhanced as the prolongation of duration in some of outer hair cells in the basal turn occured 180 minutes after total stapedectomy. (2)Significant CAP threshold shift and irreversible pathological changes in some outer hair cells in the basal turn of cochlea were found with continuous suction for 60 minutes in auditory bulla after total stapedectomy. (3)CAP threshold shift without damage of outer hair cells could be fourd with the application of suction for 60 mimutes in auditory bulla before stapedectomy. The results suggested that opening vestibular window for longer time with suction in auditory bulla might be one of the factors to cause postoperative sensorineural hearing loss in total stapedectomy. But the suction noise might not be enough to cause postoperative sensorineural hearing loss in tympanoplasty due to its relatively low intensity.

Objective To observe the effects of Donepezil on cognitive function of delyed ephalophthy after acute carbon monoxide poisoning ( DEACMP) .Methods Eighty patients with DEACMP were randomly divided into control group ( 40 cases ) and treatment group ( 40 cases ) .The control group was given treatment of regular medicine, placebo and hyperbaric oxygenation.The treatment group was given treatment of regular medicine, Donepezil and hyperbaric oxygenation.The dose of Donepezil was 5.0 mg per night, and 90 d was a period of treatment.The cognitive function of patients was evaluated respectively by MMSE and Barthel Index rating scale ( BI) .And the results were compared between the two groups.Results Compared with before treatment, the MMSE and BI scores of the two groups after treatment were significantly increased (P<0.05 -0.01).And after the treatment, the MMSE and BI scores of the treatment group were significantly higher than those in the control group (P<0.05-0.01).Conclusion Donepezil can greatly improve the cognitive function of DEACMP patients, it is worthy to be clinical promoted.

Objective To study the clinical effects of femtosecond laser surgery and phacoemulsification in patients with cataract. Methods A total of 80 patients were randomly divided into study group and control group. 40 patients with 44 cataract eyes in control group were treated by routine phacoemulsification and 40 patients with 47 cataract eyes in control group were treated by femtosecond laser additionally. The capsulotomy diameter, capsular tension ring, capsule size and operation time difference were compared between two groups. The vision and Intraocular lens decentration rate were compared between two groups. Results The capsulotomy diameter in study group was significantly less than control group (P < 0.01). The apsular tension ring in study group was significantly more than control group(P < 0.05). The capsule size in study group was significantly less than control group (P < 0.01). The operation time in study group was significantly less than control group (P >0.05). The vision in study group was significantly better than those in control group during a year (P < 0.05) and the adverse reaction rate was significantly less (P < 0.05). Conclusion Femtosecond laser combined with phacoemulsification has a great clinical effects which improve patients′ vision and reduce adverse reaction rate makes it worth for clinical application.

Objective To evaluate the clinical value and safety of radiotherapy combined with tyrosine kinase inhibitors (TKI) in non-small cell lung cancer (NSCLC) with brain metastasis driven by epidermal growth factor receptor (EGFR) mutation.Methods A retrospective cohort of 21 non-small cell lung cancer (NSCLC) patients with EGFR mutation-driven brain metastasis was recruited.10 patients (treatment group) were randomly selected for radiotherapy combined with TKI treatment,and 11 patients (control group) were treated with radiotherapy.Tumor size was measured and clinical efficacy was evaluated by MRI in the four weeks after radiotherapy.Since then,a clinical response was evaluated every three months until disease progression,and the median overall survival time was calculated.Results The objective remission was 8 cases in treatment group and 4 cases in control group.The disease control was 9 cases in treatment group and 8 cases in control group.The median overall survival time of treatment and control groups was 11.2 months and 6.4 months,respectively.Adverse reactions in treatment group mainly contained nausea,diarrhea and rash,which all were tolerated.Conclusion Radiotherapy combined with TKI is superior to radiotherapy alone in effect and safety for treatment of NSCLC with EGFR mutation-driven brain metastasis.

Objective To investigate the clinical efficacy of thiophene tiotropium powder inhaler for postop-erative lung function recovery in lung cancer patients.Methods 80 patients of thoracic surgery lobectomy who had lung cancer were selected as research subjects.The patients were randomly divided into two groups.The observation group received tiotropium bromide powder for inhalation therapy,the control group was inhaled salmeterol /fluticasone propionate powder for inhalation therapy.Before treatment,after treatment,2d,6d of CRP,absolute neutrophil ANC, tumor necrosis factor α(TNF -α) were observed.Before treatment,after treatment 4 weeks,8 weeks of forced vital capacity (FVC),within one second force expiratory volume in FEV1 ,FEV1 percent predicted (FEV1%),FEV1 /FVC, PaO2 ,PaCO3 were detected.Results Before treatment,CRP,AND,TNF -αhad no significant differences between the two groups (all P >0.05).Postoperative 2d,CRP,ANC,TNF -αof the two groups were all increased.Postopera-tive 6d,the above indicators of the two groups were declined,and the decline of the observation group was significantly lower than the control group (t =3.163 4,3.651 8,4.025 3,all P <0.05).Before treatment,FVC,FEV1 ,FEV1%, FEV1 /FVC,PaO2 ,PaCO3 between the two groups had no significant differences (all P >0.05).4 weeks,8 weeks af-ter treatment,FVC,FEV1%,FEV1 /FVC,PaO2 of the observation group were significantly higher than the control group (t =2.041 8,4.116 7;2.043 1,3.722 8;3.056 2,4.138 6;3.227 6,5.161 9;all P <0.05),FEV1 ,PaCO3 were significantly lower than the control group (t =1.523 7,3.427 6;3.439 2,4.521 7;all P <0.05).Conclusion Tiotropium bromide inhalation powder agents has certain effect on lung function recovery for lung cancer patients after surgery,it is worthy of clinical application.