Objective To investigate the effect of valsartan combined with amlodipine in treatment of elderly patients with essential hypertension and diabetes,and to provide a reference for the development of clinical treatment. Methods 339 patients with essential hypertension complicated with diabetes in our hospital were randomly divided into 3 groups:A group,B group and C group,each group in 113 cases.A group was treated with amlodipine tablets,B group was treated with valsartan capsules,and C group was treated with amlodipine tablets combined with valsartan capsules.After 3 months of continuous treatment,blood pressure,blood glucose,blood lipids and uric acid were com-pared among the three groups before and after treatment.Results The three groups of patients before treatment, systolic blood pressure and diastolic blood pressure had no significant difference;The three groups of patients after treatment,systolic blood pressure and diastolic blood pressure were significantly lower than before treatment;After treatment in C group,the systolic blood pressure was (135.70 ±8.17)mmHg,which was significantly lower than (139.42 ±8.29)mmHg in A group and (139.52 ±7.48)mmHg in B group,the difference was statistically significant (t =3.77,3.81,all P <0.05);The three groups of patients after treatment of diastolic blood pressure had no significant difference,and there were no statistical significance among the three groups before treatment in the fasting blood glucose,fasting insulin,ISI level difference;Of A group after treatment,the fasting blood glucose,fasting insulin,ISI levels were (7.63 ±0.89)mmol/L,(16.11 ±2.39)mIU,(-4.26 ±0.35),there were no significant difference before and after treatment;Of B group and C group after treatment,fasting blood glucose,fasting insulin and ISI were significantly lower than before treatment (t =4.45,4.83,6.74,6.61,4.48,4.93,all P <0.05)and A group after treatment(t =4.23,448,5.24,515,409,3.95,all P <0.05),and the differences were statistically significant,and before and after treatment the total cholesterol,triglyceride,high density lipoprotein cholesterol and low density lipo-protein cholesterol levels among the three groups had no statistical significance;the level of uric acid of C group after treatment(282.04 ±81.31)μmol/L was significantly lower than that in A group and B group(t =4.94,4.68,all P <0.05).Conclusion Valsartan combined with amlodipine can effectively reduce the community elderly patients with primary hypertension in patients with diabetes mellitus blood pressure levels,promote the stability of blood glucose level,improve the uric acid metabolism and on renal function have protective effect.

Several signaling pathways are involved in clonal proliferation of lymphoma cells and disease progression. It has been shown that cyclic adenosine monophosphate (cAMP) signaling pathway, as an important intra-cellular messenger delivery system, is related to modulation of multiple physiological processes including cell prolifer-ation, secretion, metabolism and apoptosis. Manipulating the cAMP signaling pathway can induce cell cycle arrest and apoptosis of various malignant lymphoid cells, which involves mitochondria-dependent apoptosis and cAMP-modulated protein kinases as well as changes of intracellular mediators. In-depth research and analysis of cAMP-induced apoptosis in lymphoma cells would provide potential targets and possible therapeutic means for the treatment of lymphoma.

Objective To reveal the average pass rate of a general hospital's ethic review and common problems.Methods The records generated by the ethics committee of a hospital in 3 consecutive years were reviewed and analysed with ridit of CMH and Bootstrap statistical methods.Results The pass rate for drug clinical trial is 36.09%,medical instruments 18.42%,and subject evaluation 14.29%.Among the problems,those concerning informed consent take up 63.59%,of protocol 14.56%,of CRF 7.77% and of the others 22.820%.The domestic applicants had more problems than those abroad.Besides,the pass rate of drug trials decreased with year from 45.83% in 2006 to 19.44% in 2008,which was statistically significant (P=0.029).In terms of drug clinical trial,the pass rate of revised projects in foreign countries was significantly higher than the domestic ones (P=0.028).ConclusionEthics Committee in a hospital plays a very important role in clinical researches.And informed consent and research protocol are very important in the ethic review.

OBJECTIVE A new piezoelectric aptamer biosensor is developed for determination of IgE. The energy converters are 10MHz AT-cut quartz crystals with gold-coated electrodes. The anti-IgE aptamers are immobilized onto the surfaces of crystals gold electrodes by biotin-avidin method. METHODS The standard substance and serum were detected to find the limit of detection and specificity of the biosensor. RESULTS The piezoelectric immunoglobulin aptamer biosensor could complete the detection without nonspecific response. Under the optimized conditions, the experimental results showed that the piezoelectric biosensor had good response to IgE whose frequency shifts were linearly dependent on IgE concentration in different range. The piezoelectric aptamer biosensor had been used to detect IgE in serum, the analytical results given by this method were in satisfactory agreement with those given by chemoluminescence method, its correlation coefficient was 0.9924. CONCLUSIONS Piezoelectric aptamer biosensor for the determination of IgE is of high sensitivity, high specificity, high analysis speed, unnecessary labeling, simple operation, real-time detection, etc. It is suitable for detection of IgE and should be used for clinical detection.

Objective To evaluate the clinical curative efficacy and to explore the leaking pathway of bone cement to verte‐bral canal through treating osteoporotic vertebral burst fractures (OVBF) by using percutaneous kyphoplasty(PKP) .Methods Ac‐cording to AO classification ,45 OVBF patients with 45 vertebras in the Erdos Central Hospital from October 2005 to May 2013 were treated by using PKP .The pathway of bone cement leaking to spinal canal and intervertebral space was determined by postop‐erative CT plain scan .The postoperative vertebral height ,Cobb angle and spinal stenosis improvement were measured .The change of VAS were compared between before and after operation .Results There were 2 cases of bone cement leaking to spinal canal .The leaking passway was mainly through basivertebral foramen .The leakage of bone cement to superior intervertebral space was higher than that to lower intervertebral space .The recovery of the vertebral height ,correction of Cobb angle and pre‐and post‐operative VAS scores had statistically significant differences (P<0 .05) .The spinal stenosis rate had no statistical difference between before and after operation(P>0 .05) .Conclusion PKP for treating OVBF is not a contraindication .The main pathway of bone cement lea‐king to spinal canal is basivertebral foramen after the treatment of OVBF by using PKP ,the leakage to intervertebral space is relat‐ed with the endplate damage .

Objective: To explore and discuss the clinical curative effect on intermittent positive pressure breathing therapy of non - invasive ventilator in the treatment for chronic obstructive pulmonary disease (COPD) of elderly patients with complication of acute respiratory failure. Methods: 112 elderly patients who were COPD (all of patients were acute exacerbation period) with complication of acute respiratory failure were divided into control group (60 cases) and observation group (52 cases) as the random number table. A series of basic therapy including routine anti-inflammatory, relieving asthma and expectorant therapy were implemented for all of patient. And the non-invasive ventilator was used to implement non-invasive mechanical breathing therapy. In this therapy, patients of control group were implemented sustaining positive pressure breathing therapy, while patients of observation group were implement intermittent non-invasive positive pressure breathing therapy (1:1). The curative effect, pH value (at 6,24 and 72h), PaO2 (at 6,24 and 72h), PaCO2 (at 6,24 and 72h) and complications between the two methods were compared. Results: There was no significant difference in the general demographic characteristics between the two groups. For patients at 6, 24 and 72 h, the differences of the pH value, PaO2and PaCO2 between pre and post-treatment were significant in observation group (F=5.669, F=6.985, F=7.628, P<0.05) and control group (F=5.311, F=6.892, F=7.958, P<0.05) , respectively. The differences of the pH value, PaO2 and PaCO2 at 6h, 24h and 72h were significant (at 24h, F=6.776, F=7.098, F=7.673. at 72 h, F=7.883, F=6.983, F=7.335, P<0.05),respectively. For two groups, the differences of pH value, PaO2 and PaCO2 at pre-weaning, post-weaning 6h and post-weaning 12h were not significant. While the mechanical ventilation time and mechanical ventilation rate of observation group were significant lower than that of control group (t=3.591, x2=3.052, P<0.05). Besides, the incidences of complications including flatulence, trachea intubation, respiratory and relative pneumonia of observation group were significant lower than that of control group (x2=4.997, P<0.05). Conclusion: The new method can significantly shorten duration of breathing and duration of hospital stays. And its incidence of complication is lower while safety of treatment is relatively higher than that of traditional method.

Objective To investigate the risk factors for hip fracture in elderly people in Ningbo,in order to provide the basis for prevention and treatment of hip fracture.Methods A case-control study were conducted in study on 450 cases of hip fracture patients aged over 60 years in Ningbo and 450 subjects of control as 1 ∶ 1 matched case control study.A univariate analysis of hip fracture was performed by x2 test.Risk factors for hip fracture were analyzed by logistic regression.Results Poor self-care ability (OR=5.05),light manual labor occupation (OR=4.10),history of cerebrovascular events (OR=2.59),smoking (OR=2.14) were the risk factors for hip fracture in elderly men,while poor self-care ability (OR=11.36),light manual labor occupation (OR=2.41),osteoporosis (OR=3.19),early menopause (OR=6.89),low body mass index (OR=5.05) were the risk factors for hip fracture in elderly women.The protective factors for hip fracture was milk uptake (OR=0.29) Conclusions Early interventions for hip fracture risk factors,and prevention and treatment of osteoporosis can help reduce the occurrence of hip fracture.

Aim To investigate the effects of Vam3 on ATP-induced inflammatory response in macrophages and the underlying mechanisms. Methods LPS primed mouse peritoneal macrophages were stimulated with ATP,and IL-1βlevel in supernatants was meas-ured by ELISA.Activity of caspase 1 was measured u-sing caspase 1 activity assay kit.Reactive oxygen spe-cies (ROS )level was detected with fluorescent probe DCFH-DA.MTT assay was used to detect cell prolifer-ation,and intracellular Ca2+concentration was meas-ured using laser scanning confocal microscope.Results Extracellular ATP led to increase in IL-1βrelease, caspase 1 activity and ROS production.It also led to rapid increase in intracellular Ca2+concentration and induced cell death.These effects were inhibited by Vam3 .Conclusion Vam3 inhibits ATP-induced in-flammatory response in macrophages,which may sug-gest the blocking effect of Vam3 on caspase 1 ~IL-1βinflammatory signaling pathway in macrophages.

Objective To study the differences of clinical characteristics in the children patients with pneumonia under differ‐ent body mass indexes(BMI) to provide the basis for its clinical research .Methods 160 children patients with pneumonia in the Shandong Armed Police Corps Hospital were selected and divided into the normal BMI group (15 .00

Objective To compare 2 methods to immobilize probes on the surface of gold membrane and select the better one for aptamer immobilization of aptamer-based piezoelectric quartz crystal sensor. Methods Anti-IgE aptamers were immobilized on the gold surface of piezoelectric quartz crystal sensor with thiol method or biotin-avidin method. The changes of frequency were compared in reactions with different concentrations of IgE. Results Biotin-avidin method obtained higher frequency changes in the reactions of IgE, lower limit of detection and wider liner range than thiol method. For biotin-avidin method, there was significant linear correlation between frequency changes and the concentration of IgE with the correlation coefficient of 0.994 5. Conclusion The thiol method which is proved effective for probe immobilization on gene sensor is unfit for aptamer immobilization. Biotin-avidin method has a better ability to immobilize probe on the surfaces of aptamer-based piezoelectric quartz crystal sensor.