Tumor inhibiting protein p33ING1b is the key expressive product of inhibitor of growth 1.It plays critical role in cell multiplication ,period control ,senescence ,repair of DNA damage ,apoptosis ,and chroma-tin remodeling.The abnormal expression of p33ING1b is closely related to the occurrence and development of tumor.This paper reviews tumor inhibiting protein p 33ING1b in the research development of tumors in digestive system.

Adult ; Female ; Humans ; Laryngeal Diseases ; surgery ; Male ; Middle Aged ; Otorhinolaryngologic Surgical Procedures ; methods ; Thyroid Cartilage ; surgery

Adult ; Facial Nerve Injuries ; etiology ; Foreign Bodies ; complications ; Humans ; Male ; Neck ; Parotid Gland

Acupuncture Points ; Diagnosis, Differential ; Humans ; Medicine, Chinese Traditional

Antidepressive Agents, Tricyclic ; therapeutic use ; Biomedical Research ; methods ; Depression ; drug therapy ; etiology ; Drug Therapy, Combination ; Drugs, Chinese Herbal ; therapeutic use ; Humans ; Integrative Medicine ; methods ; Phytotherapy ; Stroke ; complications

Alveolar Bone Loss ; prevention & control ; Alveolar Process ; surgery ; Bone Regeneration ; Dental Implantation, Endosseous ; Dental Prosthesis, Implant-Supported ; Guided Tissue Regeneration, Periodontal ; methods ; Humans ; Immediate Dental Implant Loading ; Male ; Middle Aged ; Periodontitis ; surgery ; Tooth Extraction ; adverse effects ; Wound Healing

Ear Canal ; pathology ; Humans ; Leukemia, Promyelocytic, Acute ; pathology ; Male ; Neoplasm Recurrence, Local ; Young Adult

Objective　To study the effects of glucose and lipid metabolism on gallstone formation. Methods　Twenty five patients with gallstones and 25 normal volunteer controls were studied from January to April in 1998. The patients were well matched the control with sex and age (1∶1). In the study, Body Mass Index (BMI) and Waist-to-Hip circumference ratio (W/H) were measured. Blood glucose, glucosylated hemoglobin (HbA1C), insulin, C peptide and all parameters of lipids were detected at fasting state. The glucose,insulin, C peptide were detected again at 2-hour after taking 75g glucose orally. Results　The result showed there was no difference on BMI and W/H between the patients and controls. HbA1C、mean fasting and 2-hour glucose concentration were not in significantly different between the two groups (P>0.05, P>0.2, P>0.1 respectively). There were 10 patients with abnormal glucose metabolism (7 with NIDDM, 3 with IGT), but only 4 controls were abnoumal (one with NIDDM, three with IGT). The difference was significant (P<0.05). Furthermore, the mean fasting and 2-hour insulin concentration of gallstone group was higher than that of the control (P<0.02, P<0.05). And the gallstone group had a higher fasting C peptide concentration than control (P<0.05). There was no statistical difference on the parameters of plasma lipid between the tow groups. Conclusion　The study suggests that diabetes mellious and hyperinsulinemia acted as an important role on gallstone formation.

Objective To investigate the expressions and clinical significance of HMGB1 and mutp53 in bile duct carcinoma tissue.Methods The expressions of HMGB1 and mutp53 were detected by immunohistochemistry in 47 cases cholangiocarcinoma tissue and 25 cases normal biliary duct tissue,and their correlation with clinicopathological parameters of cholangiocarcinoma was analyzed.Results The expression of HMGB1 and mutp53 was positive in 78.72％ (37/47) and 63.83％ (30/47) respectively of the cases with cholangiocarcinoma tissue,and 12.00％ (3/25) and 4.00％ (1/25)respectively of the cases in normal biliary duct tissue(all P ＜0.01).The expression of HMGB1 and mutp53 in cholangiocarcinoma tissue was relating to degree of tumor differentiation,lymph node metastasis,perineural invasion and TNM-staging(all P ＜ 0.05),and no relation to age,gender,serum bilirubin level,metastasis of tumor and site of tumor(all P ＞0.05).The expression of HMGB1 was positively correlated with that of mutp53 in bile duct carcinoma tissue(r =0.574,P ＜ 0.05).Conclusion The expressions of HMGB1 and mutp53 were increased in cholangiocarcinoma tissue,both of them play critical role for the occurrence,development,invasion and metastasis of cholangiocarcinoma.

Objective:To express human interferon-?2b gene and to explore the feasibility of expressing human gene in plant cells.Methods:The hIFN-?2b coding sequence was amplified by PCR with specific primers and plasmid pBV889 was used as a template,subcloned into middle vector pMD18-T and binary vector pBI121 to obtain plant expression vector pBIFN. The pBIFN was transformed into Agrobacterium tumefaciens strain LBA4404. Then hIFN-?2b gene was introduced into Ginseng callus cells via Agrobacterium-mediated transformation. The positive cells were screened by G418. The transgenic Ginseng calli were confirmed by PCR,RT-PCR,Western blot and WISH/VSV system.Results:Stable integration of the hIFN-?2b gene in the Ginseng callus cells′ genome was confirmed by PCR analysis. RT-PCR analysis showed that there were transcription products. Western blot implied that the given protein was hIFN-?2b. WISH/VSV system assay showed that the expressed hIFN-?2b possessed relatively lower bioactivity.Conclusion:HIFN-?2b has been expressed in transgenic Ginseng calli, which facilitates further investigation of improving the curative effect of orally administered hIFN-?2b.