BACKGROUND: It is very important to explore an optimal loading force that can both allow effective intrusion and cause no damage to the teeth.OBJECTIVE: To investigate the optimal loading conditions, including loading force values and directions, in the intruding mechanics of maxillary anterior teeth.METHODS: A three-dimensional finite element model (3D FEM) of the maxillary anterior teeth was constructed to include the periodontal ligament (PDL), alveolar bone, and appliance (brackets and wire). The initial stress distribution and force of the maxillary anterior teeth were analyzed when the 3D FEM was loaded with different intruding force values and directions. RESULTS AND CONCLUSION: The optimal value of the intruding force for 6 anterior teeth fell within the range from 0.5 N to 1.0 N. The maximum stress was detected at the distal cervical level of the lateral incisor, and a relatively even distribution of initial stress was observed at 20° palatal deviation to the Y axis. The results suggest that using mini-screw implants at the labial region between the canine and lateral incisor and a combined 0.5-1.0 N intruding force with a distal force could be an optimal intrusion force system by which patients with normal occlusion could obtain a pure intrusion of anterior teeth.

[Objectives]This study was designed to evaluate the treatment effects of a new movable molardistalization appliance for distal movement of maxillary first molars on Angle's Class Ⅲ malocclusion adolescence patients with the second premolar crowded.[Methods]Sixteen patients were treated by a new movable molardistalization appliance.Cephalometric analysis and model measurement was carried out before and after the treatment.Data were analyzed by paired t-test.[Results]The molars were distalized 5 mm during an average time of 3.8 months.There were significant increases in the Angle SNA and A-NPg(P ＜0.05).[Conclusion]it was a effective way to get space and improve patient's profiles on Angle's Class Ⅲ malocclusion adolescence patients with the second premolar crowded by the new movable molardistalization appliance.It was also conducive to patient's oral hygiene.

AIM: To investigate the effects of polidatin on phospholipase A_2(PLA_2),nitric oxide(NO),and endothelin-1(ET-1) levels in serum and lung homogenate of hypoxic pulmonary hypertension rats.METHODS: 29 healthy Spraugue-Dawley rats were randomly divided into normal control group,chronic hypoxic group and hypoxic plus polidatin group.The model of chronic hypoxic pulmonary hypertension was made by method of intermittent isobaric hypoxia for 21 days.The mean pulmonary arterial pressure(mPAP) was measured by inserting a microcatheter into the pulmonary artery.The ratio of right ventricular wall and that of left ventricular wall and ventricular septum weight(R/L+S) was measured as well.RESULTS: After exposed to hypoxia for 21 days,the mPAP,R/L+S,PLA_2 and ET-1 activities in blood and lung homogenate increased significantly. The NO concentration decreased significantly.Pretreatment with PD attenuated these changes mentioned above.CONCLUSION: Pretreatment with PD effectively prevents the formation of chronic hypoxic pulmonary hypertension.Its mechanism may be related to the inhibition of PLA_2 and ET-1 and promotion of NO production.

Objectives To study the changes of hard tissue before and after orthodontic treatment for Angle's class Ⅲ malocclusion adolescence patient with maxillary front traction. Methods 20 cases of Angle's class Ⅲ malocclusion adolescence patients were treated by front traction in maxilla, while there were 20 cases of Angle's class Ⅰ malocclusion adolescence patients as the control group. Cephalometric a-nalysis was carried out before and after the treatment. Data were analyzed by SPSS11.0 system using paired t-test. Results There were significant increases in the length, anteroposterior diameter and thick-ness of maxillary sinus (P<0.05), while there was no significant differences in the site and central point of maxillary sinus(P>0.05). Conclusions It is a effective approach to change the hard tissue after ortho-dontic treatment for Angle's class Ⅲ malocclusion adolescence patient with front traction in maxillary area.

Objective To explore the necessity of graded rehabilitation interventions for stroke by means of a retrospective analysis of the destination and functional outcome of stroke patients.Methods One hundred and twenty-one stroke patients discharged from the authors' department in a tertiary hospital after hospitalized rehabilitation treatment and continued with no guided rehabilitation training at home for a period of more than 15 days before readmission to the department were recruited as the family group,another 47 patients discharged from the same department and continued with hospitalized rehabilitation treatment in other institutions for a period of more than 15 days before re-admission to the department were recruited as the rehabilitation group.The demographic data,the patients' Barthel index scores Holden gait classifications at discharge from the authors' department and at re-admission of both groups were analyzed and compared.Results The complications in rehabilitation group were much less than in the family group.The difference of Barthel index scores between previous discharge and re-admission in family group was negatively correlated with the age of stroke onset,number of complications,and was positively correlated with the Barthel index score and Holden classification at the previous discharge.It was also found that the patients in family group would remain stable with their ADL performance and gait if their BI score was equal or above 45 and Holden classification equal or above grade 2 at the previous discharge.Conclusion Stroke patients in tertiary hospitalized rehabilitation program should be discharged either to secondary institution or family in accordance to their functional status.

OBJECTIVETo analyze the expression of foreign gene in the filial generation of the transgenic plants on the base of the original transgenic tomatoes seeds carrying the gene encoding saliva-binding region (SBR) in PAc of Streptococcus mutans (S. mutans) gained.

METHODSThe tomatoes total DNA was extracted by CTAB methods, and the filial generation transgenic tomatoes carrying the gene encoding SBR in PAc of S. mutans were selected by PCR. The tomatoes total RNA was extracted by trizol and the transcription of the foreign gene was analyzed by RT-PCR. Protein was extracted from fruit tissue and the content of the total protein was determined by Bradford's methods G250. The expression of foreign protein was analyzed by Western blot and the lever of the foreign protein was analyzed by ELISA.

RESULTSThe fragment encoding SBR in S. mutans PAc gene integrated in the tomato genomic DNA and was expressed. The foreign protein lever was up to 1.2% of the total soluble protein in tomato fruit tissue.

CONCLUSIONThe foreign protein gene in the filial generation of the transgenic plants could express the foreign protein.

Objective:To analyze the feature of cranial nerve involvement in nasopharyngeal carcinoma (NPC) and its relationship with the prognosis.Method:A total of 1892 patients who were diagnosed as NPC in our hospital from January 2002 to December 2003,of which the cranial nerve involvement was 183 (9.6%) patients, were analyzed the effect of cranial nerve involvement on the prognosis.Result:The percentage of cranial nerve involvement was 9.4%. The 5 year overall survival rate was 61.0%,disease free survival rate was 55.3%,local relapse free survival rate was 75.2% and distant metastasis free survival rate was73.4%.Periods of cranial nerve involvement,clinical stage,the diameter of the lymph nodes,involvement of cavernous sinus, and the level of the recovery of cranial nerve involvement were significantly associated with prognosis in univariate analysis(P<0.05).With multivariate analysis,the recovery level of cranial nerve involvement was the independent factor that affected the 5-year overall survival (RR=2.087). The diameter of the lymph nodes and involvement of cavernous sinus were the independent factors that affected the 5-year distant metastasis-free survival(RR=1.954 and 2.136,respectively).Conclusion:Periods of cranial nerve involvement and the level of the recovery of cranial nerve involvement were significantly correlated with prognosis. Involvement of cavernous sinus could increase the rate of distant metastasis.

Objective To investigate the relationship between glucokinase regulator protein ( GCKR) gene polymorphism rs780094 and hyperuricemia in Uygur in Xinjiang. Methods A case-control study including 1 026 patients with hyperuricemia and 1 030 normal subjects was conducted. All the subjects were genotyped for GCKR gene rs780094 by Sequenom MassARRAY system. The results of rs780094 genotype and allele frequency between hyperuricemia group and control group were compared. The associations of different genotypes of rs780094 with blood pressure, blood lipid, and blood glucose were analyzed. Logistic regression analysis was used to analyse the relationship between polymorphism of rs780094 and hyperuricemia in Uygur in Xinjiang. Results The distributions of three genotypes(G/G, A/G, A/A) and two allele frequency (G and A) in GCKR rs780094 revealed statistical difference ( P<0. 05 ) between hyperuricemia group and control group. A tendency toward association with hyperuricemia was observed under dominant model(OR=1. 295, 95%CI 1. 078~1. 554,P=0. 006) and recessive model(OR=1. 284, 95% CI 1. 024 ~1. 611,P=0. 030). The levels of systolic blood pressure, diastolic blood pressure, and total cholesterol were lower in hyperuricemia group with GCKR gene rs780094 loci GG genotype than those with AA+AG genotype. After adjusting confounding factors which had significant difference in the single factor analysis, logistic regression analysis showed that rs780094 A/A and A/G might be risk factors of hyperuricemia in Uygur in Xinjiang (OR=1. 355,95% CI 1. 094 ~1. 679,P=0. 005). Conclusion The GCKR rs780094 is associated with hyperuricemia in Uygur in Xinjiang. The A/A and A/G genotype of the GCKR rs780094 may increase the risk of hyperuricemia.

BACKGROUND:Distraction osteogenesis applied in patient’s canine distal movement, can greatly improve the speed of tooth movement, and protect the molar anchorage. But the researches on the traction rate, canine pulp vitality, canine periodontal tissue remodeling and the biological mechanisms of the technology are rare recently. OBJECTIVE:To evaluate the feasibility of rapid canine retraction through distraction osteogenesis of the periodontal ligament in adult patients and to monitor the pulp vitality, root resorption and periodontal remodeling of the canine. METHODS:Nine malocclusion patients were selected, and the bilateral maxil ary canine in adult patients were rapidly retracted and moved to the scheduled position using modified distraction devices. The canine distal movement distance, anchorage loss, root resorption and alveolar interval alterations were measured through intraoral radiographs, and lateral cephalograms. The pulp and periodontal of canine were mointrored. RESULTS AND CONCLUSION:The canines could be rapidly retracted to the schedualed position through distraction osteogenesis of the periodontal ligament in 12-16 days, the average amount of retraction was 7.18 mm and the canines tipped distal y (13.24±2.87)°. The anchorage loss was 0.5 mm. There was no obvious root resorption and periodontal tissue loss. The pulp vitality of the canines was decreased significantly right after distraction, but it recovered significantly after 3 months. The results indicated that periodontal ligament distraction osteogenesis can significantly accelerate canine movement speed, shorten treatment time, while protecting the molar anchorage;there was no significant adverse reaction of root resorption, loose teeth, pulp necrosis and loss of periodontal tissue. Indcating that distraction osteogenesis of periodontal ligament can move canines rapidly and effectively.

0.05).The results of electrocardiography and the laboratory ex-amination showed that neither ANWin cluding natural Calculus Bovis nor A NWincluding in -vitro cultured Calc ulus Bo-vis had obviously toxic and side effe cts in treating epidemic encephalitis B.Conclusion ANW including in -vitro cul-tured Calculus Bovis has an markedly effect in the treatment of epidemic e ncephalitis B.