Objective To investigate the associations of alcohol consumption with the outcome of three-year pre-dia?betes. Methods The objects of this study were selected from the epidemiological study of cancer risk of Chinese patients with type 2 diabetes mellitus (T2DM) in Luzhou area. A total of 1 125 cases with pre-diabetes were selected from the base?line survey. Patients divided into four groups including non-alcohol drinking group, small alcohol drinking group, moderate alcohol drinking group, and large alcohol drinking group. Patients also were divided into two groups according to body mass index (BMI, more than 24 kg/m2). The patients were followed up for three years. The incidence rates of diabetes mellitus were compared between different drinking groups and different BMI groups. Results A total of 1 116 cases were completed the follow-up , 9 cases were lost to follow-up. The incidence rates of DM were 18.81%, 8.80%, 16.51%and 26.53%for non-al?cohol drinking group, small alcohol drinking group, moderate alcohol drinking group, and large alcohol drinking group. Cox proportional hazard regression analysis showed that the relative risk ratios and 95%CI of the gender, age, BMI, calorie, base?line blood glucose were 0.49 (0.31-0.79), 1.15 (0.69-1.92) and 1.95 (1.25-3.04) for small alcohol drinking group, moderate alcohol drinking group, and large alcohol drinking group. In the group of BMI<24 kg/m2,the relative risks and 95%CI were 0.40 (0.20-0.80),0.77 (0.30-1.97) and 1.10 (0.46-2.64) for small alcohol drinking group, moderate alcohol drinking group, and large alcohol drinking group. In the group of BMI≥24 kg/m2, the relative risks and 95%CI were 0.62 (0.33-1.66),1.49 (0.80-2.80) and 2.64 (1.55-4.47). Conclusion Small amount of alcohol drinking can reduce the incidence of DM in predia?betes people with BMI<24 kg/m2. Large amount of alcohol drinking is the risk factor of incidence of DM in people with BMI≥24 kg/m2.

Objective To evaluate the cost-effectiveness of four therapeutic regimes in the treatment of pa-tients with initial vulvovaginal eandidiasis. Methods 160 patients who were found for the first time with vulvovagi-hal eandidiasis were divided into four groups randomly: group A is clotrimazole (500rag) vaginal tablets; group B is fluconazole eaptica;group C is clotrimazole vaginal tablets combined with flueonazole;group D is nystatin vaginal ef-fervescent tablets. Date was evaluated using clinic economies co,t-effectiveness analysis. Results Among four groups,the costs for group A,B,C and D were 167.20 yuan, 190.23 yuan,237.43 ynan and 216.40 yuan,respec-tively. The tatol effective rates were 90.88 %, 90.12 %, 91.67 % and 67.10 %, respectively. The cost-effectiveness ratio were 1.84,2.11,2.59 and 3.23, respectively. The incremental cost-effectiveness ratios of group B, C and D vs group A were 32.90,87.78 and 2.07. Conclusion Regime A was superior than the other therapeutic regimes.

Objective To realize SLAM and path planning of mobile robot to solve the problem of real-time data association in the process of moving and to update the computational complexity of the state covariance matrix.Methods The mathematical model of SLAM was built,and FastSlam algorithm based on particle filter,A* and dynamic window approach (DWA) were used to prepare gmapping function package in the ROS framework.Results The motion simulation,path planning and dynamic obstacle avoidance of mobile robot in the ROS framework were realized.Conclusion By using ROS platform,mobile robots can execute path planning efficiently while completing self-localization and map building.

Objective To investigate the effect of placenta derived tumor necrosis factor (TNF)-α and myostatin (MSIN) in patients with preeclampsia (PE) and fetal development.Methods One hundred and twenty pregnant women who delivery from October 2008 to October 2013 were enrolled in this study.In them,40 healthy pregnant women was normal control group,40 PE and fetal growth in normal pregnant women was PE group,40 PE and fetal growth restriction (FGR) of pregnant women was PE + FGR group.The immunohistochemical localization of SABC method was used to detect for TNF-α and MSIN protein in placenta tissue in each group respectively.Real-time fluorescent quantitative PCR and Western blotting were used to detect for TNF-α and MSIN mRNA and protein in placenta tissue.Results The TNF-α mainly located in placental blood vessels surrounding stroma,decidual cells,trophoblastic cells and MSIN mainly located in placental blood vessels surrounding stroma,decidual cells and terminal villi.The TNF-α and MSIN mRNA expression quantity in PE group was 3.65 ±0.86,1.80 ±0.32 ; in PE + FGR group was 3.88 ± 0.71,2.01 ± 0.55 ; in normal control group was 1.32 ± 0.21,0.77 ± 0.39.The TNF-α and MSIN mRNA expression quantity in PE group and PE + FGR group were significantly higher than those in normal control group(P ＜ 0.01),and there were significant differences between PE group and PE + FGR group(P ＜ 0.05).The TNF-α and MSIN protein expression in normal control group was 0.56 ±0.13,1.31 ± 0.23;in PE group was 1.67 ±0.25,1.55 ±0.34 ;in PE + FGR group was 2.78 ±0.41,3.07 ±0.51.The TNF-α and MSIN protein expression in PE group and PE + FGR group were significantly higher than those in normal control group(P ＜ 0.01),and there were significant differences between PE group and PE + FGR group(P ＜ 0.05).Conclusions The placenta derived TNF-α and MSIN have more important roles in the pathogenesis of PE and normal development of fetal.It can provide coping strategies by detecting the level of placenta derived TNF-α and MSIN.

Objective To design a chock-vault antenna for injurious interventional thermotherapy and to evaluate antenna performance change when structure parameters of the antenna are adjusted. Methods Finite element method was used to simulate the distributions of the reflection coefficient and the specific absorption rate (SAR) of the chock-vault antenna of different structure parameters for microwave thermotherapy human muscle tissue model when it worked at 2 450 MHz. The chock-vault antenna for injurious interventional thermotherapy had been optimized. Results This antenna was made.And the reflection coefficient and SAR were measured with human muscle tissue model experiment. The simulation results were validated. Conclusion The SAR distribution of the improved choke-vault antenna does not depend on depth of the insertion tissue. The reflection coefficient can be reduced greatly. The top energy distribution is reasonable.

Objective:To explore the expression of IL-22 in the patients with rheumatoid arthritis,and to define the clinical sig-nificance of IL-22 cells for RA. Methods: A total of 50 RA patients, 15 OA patients and 15 healthy controls were enrolled. The proportion of Th22 cells in peripheral blood and synovial fluid( SF) of RA patients was detected by flow cytometry;the levels of IL-22 in serum and synovial fluid of RA patients were detected by ELISA. The clinical parameters of disease activity were assessed including ESR,RF,CRP,DAS28,anti CCP and the degree of bone erosions determined by X-rays. Pearson correlation analysis,t test and Kruskal-Wallis H test were used for statistical analysis. Results:The proportion of Th22 cells in RA patients was higher than that of OA patients (t=2. 290 ,P=0. 021) and healthy controls(t=2. 524,P=0. 015). IL-22 levels in RA patients were higher than that of OA patients (t=2. 560,P=0. 014) and healthy controls(t=2. 768,P=0. 009). IL-22 in the RF positive group were higher than that of RF negative group(t=2. 322,P=0. 035). IL-22 in the anti-CCP antibody positive group were higher than that of anti-CCP antibody negative group (t=2. 504,P=0. 015). The levels of IL-22 were correlated positively with ESR,RF,DAS28(r=0. 312,0. 314,0. 332,P<0. 05). The levels of IL-22 were increased gradually along with the aggravation of bone erosions. There were statistical significant difference among different X-ray stagings(H=9. 14,χ20.05(3)=7. 81,H> χ20.05(3),P<0. 05). Serum IL-22 levels in the RA patients with joint effusion were higher than that of without(t=2. 587,P=0. 012). The levels of IL-22 in SF were higher than that in serum(t=2. 668,P=0. 011),and had no correlation with the proportion of Th22 cells in SF. Conclusion: The expression of IL-22 in serum and joint effusion of RA patients increase. The level of IL-22 may be useful marker for assessment of disease activity and finding of bone erosions. Therapeutic targeting of IL-22 may be valuable for the intervention of RA.

The validity of (99m)Tc-YIGSR, a novel receptor radio-tracer, in imaging the Ehrlich ascites tumor was evaluated. YIGSR, a pentapeptide of laminin, was labeled with (99m)Tc by using a bifunctional chelator S-Acetly-NH(3)-MAG(3). The MIBI was labeled with (99m)Tc by following the kit instruction. The mice of tumor group were intravenously injected 1-2 mCi of (99m)Tc-YIGSR or (99m)Tc-MIBI via caudal vein, immobilized and imaged under a Gamma camera. The same procedure was performed in mice of blockade group, in which the unlabeled YIGSR was previously injected to block the receptor-recognition sites, and inflammation group serving as control. The reverse-phase Sep-Pak C(18) chromatogram was found to have an essentially complete conjugation between YIGSR and S-Acetly-NH(3)-MAG(3). The conjugated YIGSR could be radio-labeled successfully with (99m)Tc at room temperature and neutral pH, with a radio-labeling yield of 62%. Without the chelator S-Acetly-NH(3)-MAG(3), the YIGSR was labeled with (99m)Tc at an efficiency of 4%. The imagological study revealed obvious tumor accumulation of (99m)Tc-YIGSR 15 min after the injection, and the uptake peaked after 3 h with a tumor-to-muscle ratio (T/M) of 11.36. The radio-tracer was slowly cleared up and resulted in a T/M of 3.01 at the 8th h after the injection. As for blocked group, the tumor uptake of radiotracer was significantly lower, with the highest T/M being 4.61 after 3 h and 0.89 after 8 h. The T/M was 3.72 at the 3rd h and 1.29 at the 8th h after the (99m)Tc-YIGSR injection in the inflammatory group. The T/M was significantly higher in tumor group than in inflammatory group or control group (P<0.001). In the 99mTc-MIBI group, the T/M was 1.40 at the 3rd h and 0.55 at the 8th h after the injection, which showed a significant difference as compared with (99m)Tc-YIGSR (P<0.001). It is concluded that YIGSR can be successfully radiolabelled by using S-Acetly-NH(3)-MAG(3). (99m)Tc-YIGSR has many advantages in tumor imaging, such as quick and clear visualization, high sensitivity and specificity, and satisfactory target/non-target ratio (N/NT). It promises to be tumor radio-tracer.
Carcinoma, Ehrlich Tumor/*radionuclide imaging ; Radioactive Tracers ; Radiopharmaceuticals/*diagnostic use ; Receptors, Laminin/*metabolism ; Technetium Tc 99m Mertiatide/*diagnostic use ; Technetium Tc 99m Sestamibi/*diagnostic use

Caenorhabditis elegans is a very important model organism in life sciences. C. elegans has been widely used in research on life sciences, especially in drug screening and the mechanism of drugs, thanks to some of their prominent characteristics, including a short life,short generation cycle, and easy culture and observation. Aging is a complex process, which is the result of multiple factors. There are mainly three types of anti-aging signal pathways in C. elegans, including insulin-insulin-like growth factor-1 signal pathway, diet-restricted signaling pathway and mitochondrial respiratory chain/ATP synthesis pathway. In this paper, we reviewed the aging models based on the above three signaling pathways and the progress in anti-aging drugs based on the above aging models. In addition, a number of C. elegans models of aging-related neurodegenerative diseases can be obtained by using transgenic or chemical mutagenesis. Thus, this paper reviewed the transgenic models of C. elegans associated with neurodegenerative diseases, including theα-synuclein transgenic model of Parkinson disease, theβ-amyloid deposition model of Alzheimer disease, and the polyQ of Huntington disease, and summa?rized the effective drugs based on the above disease models. This review will provide reference for the study of C. elegans in the future screening of anti-aging drugs and drug screening for the prevention and treatment of neurodegenerative diseases.

Objective To explore the A2AR activation after traumatic brain injury mechanisms and the role of excessive tau protein phosphorylation.Methods With no specific mice experiment research of specific pathogens, position in the left parietal cortex in mice, by the method of controllable cortical against brain trauma model model, 15 min after injury in mice abdominal injection of A2AR specific inhibitors ZM241385 or use A2AR knockout mice, testing the brain neuron loss and tau protein phosphorylation level;Use specific agonists CGS21680 activate the original generation of nerve cells in the hippocampus and the A2AR human neuroblastoma cells, using immunocytochemistry and immunofluorescence test tua protein phosphorylation level of change, to observe axon transport function of mitochondria.Results Immunohistochemical results accumulation of optical density analysis showed that inhibition of A2AR activation can significantly reduce after cerebral trauma Ser404 tua protein loci phosphorylation levels, reduce excessive tua protein phosphorylation with nerve pathological change;A2AR activation after tua phosphorylation of proteins at a Ser404 site level increased significantly, nerve axons per unit length processes in the mitochondria number decreased significantly, resulting in axoplasmic transport dysfunction;To activate the original generation of nerve cells in the hippocampus and after the A2AR human neuroblastoma cells, tua protein phosphorylation Ser404 locus levels increased significantly.Conclusion A2AR activation after cerebral trauma has obvious influence on tua protein phosphorylation levels, may be a function by influencing the axoplasmic transport, eventually forming cognitive dysfunction.