Objective To study the clinical characteristics and imaging features of alien hand syndrome (AHS). Methods We retrospectively analyzed the clinical data of 3 typical cases in our hospital,in comparison with case reports and reviews of domestic and foreign during the past decade. Result All 3 patients showed corpus callosum lesions from CT or MRI detections. The symptoms of the patients included alien hand sign , abnormal involuntary movements, intermanual conflict of upper limbs, and so on. And the patients accompanied with emotional and cognitive changes. Two of them were improved in different degrees after treatment. Conclusion AHS was a complex disease. The diagnosis of this syndrome was based on the advanced neuroimaging. There was still no well established treatment for AHS.

AIM: To establish the quality standard for Compound Songluo Granule (Rhizoma Corydalis, Radix Angelicae Sinensis, Lichen Usneae). METHODS:Rhizoma Corydalis, Radix Angelicae Sinensis were identified by TLC. Usnic acid in Lichen Usneae was determined by TLCS. RESULTS: Rhizoma Corydalis, Radix Angelicae Sinensis could be detected by TLC. Usnic acid showed a good linear relationship at the range of 0. 50～2.50 ?g, r=~0.999 9. The average recovery was 97.5% and RSD was 2.61%, respectively. CONCLUSION:The method is available with a good reproducibility and can be used for the quality control of Compound Songluo Granule.

Angioplasty, Balloon, Coronary ; Coronary Restenosis ; prevention & control ; Endothelial Cells ; cytology ; Endothelium ; cytology ; Humans ; Stem Cells ; cytology ; Stents

Thyroid-stimulating hormone receptor(TSHR)is an important autoantigen whose cleavage and shedding can induce autoimmunity.The TSHR is the main functional receptor of the thyroid.The shedding of the ? subunit plays an important role in the forming of TSAb and balancing between TSAb and TBAb.Research on the cleavage and shedding of the TSHR extracellualar domain helps to gain a deep insight into the structure and function of TSHR,illuminate the pathogenesis of autoimmune thyroid disease and find out a new therapy.The structure of TSHR,the mechanism and the significance of its cleavage and shedding are reviewed in this article.

Objective To investigate alterations in epigenetic regulation in B cells in subacute cutaneous lupus erythematosus (SCLE).Methods Peripheral blood mononuclear cells (PBMC) were isolated from the peripheral venous blood of SCLE patients and healthy donors with density gradient centrifugation.B cells were isolated with CD19 micro beads and protocols provided by the manufacturer.Total RNAs were isolated with RNA kits.The expressions of silence information regulator 1 (Sirt1) mRNA were investigated with quantitative real time polymerase chain reaction (PCR).Results The levels of Sirt1 mRNA were significantly increased in SCLE B cells relative to healthy controls (P ＜ 0.01).Conclusions Histone modifications appear abnormal in B cells in SCLE.

Objective To optimize the method and condition s for the extraction process of Fushuning Granules.Methods Different factors and levels were in vestigated by orthogonal design wit h yields of oleanolic acid and tanshi noneⅡ A as the markers.The yields of effective com ponents by percolation and reflux we re compared.Results The extraction ratio by re-flux was higher than that by percolation.The primary factors influencin g extraction ratio by reflux were the pulverization degree of medicinal materials,concentration of alcohol,refluxing times and refluxing perio d.Conclusion The opti-mum extraction process was:refluxing and extracting the pulverized med icinal materials for 3times(1hours each time )with 6-fold 90%alcohol added in the f irst time and then 5-fold in the secon d and the third time.

Objective To explore the effect of advanced glycation end products(AGEs) on the oxidative stress in porcine vascular endothelial cells(PIECs). Methods After being intervened by AGEs for some time,cell viability was detected by MTT.2′,7′-dichlorofluorescein diacetate(DCFH-DA) was used as a reactive oxygen species(ROS) capture agent.The fluorescent intensity of 2′,7′-dichlorofluorescein(DCF),which was the product of cellular oxidation of DCFH-DA,was detected by flow cytometry,and the level of ROS was thus measured. Results Viability of PIECs was inhibited by AGEs in a dose-and time-dependent fashion(P

ABSTRACT:Objective To investigate the anti-metastatic effect of allicin on glioma cell line U87 and related mechanisms.Methods In this study,we employed MTT assay to test the anti-proliferative effect of allicin. Transwell assay was used to test the anti-metastatic ability of allicin.Real-time PCR and Western blotting were employed to test the effect of allicin on the expressions of matrix metalloproteinase-2 (MMP-2 ) and matrix metalloproteinase-9 (MMP-9).Western blotting was employed to test the phosphorylated level of p38.Results Allicin could significantly inhibit the proliferation and invasion of U87 cells (concentration>8 μg/mL,P <0.05). Meanwhile allicin (concentration<8μg/mL)could inhibit the invasion of U87 cells.After treatment with allicin for 24 hours,the expressions of MMP-2 and MMP-9 were decreased significantly (P < 0.05 ).Moreover,allicin treatment decreased the phosphorylated level of p38 obviously (P < 0.05 ).Conclusion Allicin inhibits the invasion and migration of glioma cell line U87 by reducing the expressions of MMP-2 and MMP-9 via suppressing the activity of p38 signal pathway,suggesting that allicin is a potential therapeutic agent for glioma.

75 years old) with calculous acute cholangitis were endoscopically treated from May 1998 to June 2005.The endoscopic nasobiliary drainage(ENBD) was firstly performed.After patient's conditions had relieved,basket stone removal,endoscopic common bile duct exploration,or endoscopic retrograde biliary drainage(ERBD) were carried out respectively according to patient's individualized conditions.Results The ENBD was performed successfully in all the 273 patients.Three patients died before further treatment.Out of the remaining 270 patients,complete removal of common bile duct stones was achieved by single-session of emergent endoscopy in 36 patients,and by secondary duodenoscopy in 178 patients.ERBD was carried out in 31 patients.Common bile duct exploration with the combination use of duodenoscopy,laparoscopy,and choledochoscopy was conducted in 25 patients,including a conversion to open surgery in 1 patient.No serious complications were found in all the patients.Conclusions For senile patients with calculous acute cholangitis,emergent ENBD combined with duodenoscopic stone removal is safe and effective.

Objective To study the most appropriate approach of combination use of endoscopy and laparoscopy in the treatment of intra and extra-hepatic bile duct stones.Methods A total of 1 669 patients with intra-and extra-hepatic bile duct stones were treated from October 2000 to December 2005 in this hospital.According to patient's individual conditions,different surgical schemes were employed,including duodenoscopy only,combination of laparoscopy and duodenoscopy(or choledochoscopy),combination of duodenoscopy,laparoscopy,and choledochoscopy,and hand-assisted laparoscopic hepatectomy.The success rate and post-operative results were observed and analyzed.Results The combination use of endoscopy and laparoscopy was successfully carried out in 1 636 patients,the total success rate being 98.0%(1636/1669).There were no serious post-operative complications.Conclusions Combination use of endoscopy and laparoscopy can be employed for various conditions of patients with intra-and extra-hepatic bile duct stones.The technique integrates advantages of both digestive endoscopy and laparoscopy,broadens indications of minimally invasive treatment,and improves the success rate.