Inhalation chemotherapy and immunotherapy is a developed technique for respiratory neoplasm recently. It is easy manipulated with high local response and little side effects. The drugs include 5 FU,DDP, taxol, camptothecin,interleukin 2 and GM CSF, etc. Inhalation chemotherapy and immunotherapy have been successfully applied for the therapy of primary lung cancer as well as the lung metastases other tumor.

OBJECTIVE:To improve the quality of pharmaceutical care and to provide bases for outpatients and medical staff in drug informatin inquiry.METHODS:Information about drug package inserts wre collected and the drug-inquiry system was designed using the database management function of Access.RESULTS & CONCLUSIONS:The drug-inquiry system is easy to operate,clear in interface,humanization in operation,and it faciliats individualized drug information inquiry through daily mantinance and satisfies the requirments for outpatient drug information inquiry.

Objective To analyze the clinical features of acute hyperlipidemic panereatitis,and to investigate the therapeutic strategies.Methods In this retrospective study,44 patients with hyperlipidemic pancreatitis admitted to our hospital from January 2003 to December 2007 were enrolled,60 patients with acute pancreatitis of other etiologies were enrolled as the control group.Results The proportions of patients with overweight or obesity,hyperglycemia,fatty liver and hypertension were 81.8%,59.1%,54.5% and 68.2%,respectively;these were significantly higher than those in control group,which were 16.7%,16.7 %,13.3 % and 23.3 %,respectively (P＜0.05 or＜0.01).The proportion of patients with lithiasis was lower in HLP group than that in control group (13.6% vs 60.0%,P＜0.05).There was no difference in the proportions of patients with chronic alcoholism between two groups.The Ranson score,CTSI,complications in HLP group were 3.15±0.07,4.46±2.58 and 3.2±1.7,respectively;these parameters were significantly higher than those in control group,which were 1.62±0.22,2.62±1.90 and 0.9±1.2 (P＜0.05 or ＜ 0.01 ).The level of serum amylase in HLP group was 580±222 mmol/L,which was significantly lower than that of control group (1361±472 mmol/L,P ＜ 0.01 ).The triglyceride (TG) level was linearly correlated with Ranson score in HLP group ( r = 0.77,P ＜ 0.05 ),but there was no linear correlation between TG level and Ranson score in the control group.Conclusions There was a close relationship between HLP and metabolic syndrome.Serum TG was positively correlated with the severity of HLP.

Objective: To investigate the effect of tumor specific antigen of HCA520 on the proliferation of HEK293 cells and to obtain some functional implications for HCA520. Methods: MTT assay was performed with HEK293 stable cell lines transfected with pcDNA3-HCA520-flag construct. Cytokines probably involved in HCA520 enhanced proliferation were screened by RT-PCR, and effect of these cytokines on the HEK293 cell proliferation was further confirmed by MTT assay. Results: HCA520 significantly promoted the proliferation of HEK293 cells, which was at least partially attributed to the up-regulation of IL-6 in HEK293 cells by HCA520. Conclusion: HCA520 might accelerate tumorigenesis by promoting proliferation of cancerous cells.

We used retroviral vector pLXSN to construct recombinant retroviral vectors with the human apoptosis gene, interleukin-l? converting enzyme (ICE). The vectors were introduced into packaging cell line PA317 by electroporation method. The G418 resistant colonies were selected, and the supematants of the colonies were used to infect the human hepatocellular carcinoma cell line SMMC7721. G418 resistant colonies of SMMC7721 were named SMMC7721-MCE and SMMC7721-neo. The results of RT-PCR analysis showed that exogenous hICE gene had successfully integrated into the genome of SMMC7721-hICE cells. The proliferation rate and tumorigenicity of cells in nude mice were examined. Our data showed that the growth rate and the tumorigenicity of SMMC7721-hICE cells in nude mice were considerablely decreased comparing with parent SMMC7721 and SMMC7721-neo. These results suggested that the retroviral vector expressing hICE gene was successfully constructed and could suppress the growth ability and tumorigenicity of human hepatocellular carcinoma cells, which provided a basis for further investigation of hICE gene therapy.

Erythrocytes, the most abundant cells in human body with excellent physiologic and morphologic characteristics, have been exploited extensively for their potential applications as drug delivering microspheres for several potential applications, including intravenous slow release of therapeutic agents, enzyme replacement therapy and drug targeting therapy. A series of methods has been tested successfully for the improvement of targeting to reticuloendothelial system.

0.05), but they did at 48 h(P

A number of medications have been proved to be able to either improve the antitumor effect of chemotherapeutics and molecular targeted drugs or reverse the resistance of tumors to chemotherapeutics and molecular targeted drugs,which are not traditionally used as anticancer drugs.Especially for late-stage tumors after multiple treatments,these agents are good alternatives when used independently or in combination with chemotherapeutics and molecular targeted drugs.These drugs include proton modulators,hypoglycemic agents and cardiovascular agents,etc.

Objective:To establish an HPLC method for the determination of related substances in enalapril maleate dispersible tablets.Methods:AKromasil100-SC8(250mm×4.6mm,5μm)columnwasused. ThemobilephaseAwas0.01mol·L-1potassi-um dihydrogen phosphate (adjusting pH to 2. 2 with phosphoric acid) and mobile phase B was acetonitrile with gradient elution,and the flow rate was 1. 0 ml· min-1 . The detection wavelength was 215nm,the column temperature was 40℃,and the injection volume was 20 μl. Results:The linear range of enalaprilat,enalapril maleate and eanlapril diketopiperazine was 14.64-43.92 μg·ml-1(r =0. 999 8), 9. 29-27. 87 μg·ml-1(r=0. 999 1)and 10. 80-32. 40 μg·ml-1(r=0. 999 5), respectively. Enalaprilat was not detec-ted out and enalapril diketone was 0. 2% using the method described in Chinese pharmacopeia, and the content of enalaprilat was 0. 1% and that of enalapril diketone was 0. 2% using the gradient elution. The related substances and enalapril maleate could be sepa-rated well. Conclusion:The method is sensitive, accurate and highly specific in the quality evaluation and control.

Purpose:To detect the action of arsenic trioxide(As 2O 3) on the expression of tumor drug-resistant protein. Methods:APL cell line MR 2 resistant to all-trans retinoic acid(ATRA) was used for in vitro studies. APL cell line NB 4 was used for control. The expressions of P-glycoprotein(Pgp), multidrug resistance protein(MRP)were determined by immunocytochemical assays. Results:The expression of Pgp was significantly higher in MR 2 cell line(30%-40%) than in NB 4 cell line(10%-20%)(P