Objective To investigate the influences of carbon dioxide (CO2) pneumoperitoneum and operation position on intraocular pressure (IOP) during laparoscopic surgery under general anesthesia. Methods Thirty-six patients were divided into two groups of udergoing laparoscopic pelvic surgeries (group A) and udergoing laparoscopic cholecytectomies(group B) with 18 cases each. The patients in group A were operated under 25 degrees head-down position and those in group B under 25 degrees head-up position. IOP was recorded before surgery(T0), at 5 min after tracheal intubation (T1), 5 min after CO2 pneumoperitoneum(T2), 5 min(T3) and 30 min(T4) after body position changed and the time of normal PETCO2 regained(T5). Results IOP was remarkably decreased after induction of general anesthesia in both groups. IOP of group A raised to (21.0±1.6) mm Hg at T4, which was dropped to (14. 6±1.6) mm Hg at T5. IOP of group B raised to (12.1±2.9) mm Hg at T4,which was dropped to (11.1±1.2) mm Hg at T5. Changes of IOP of both groups were positively correlated with the changes of PETCO2. Conclusion IOP can be decreased remarkably after induction of general anesthesia with propofol. The head-up position and normal range of PETCO2 are the two important factors to avoid an increase of IOP in surgical patients under general anesthesia.

Objective To evaluate the efficacy of seretide (fluticasone/salmeterol) in treatment of patients with cough variant asthma(CVA). Methods 86 CVA patients were treated with fluticasone/salmeterol propionate 8 weeks, after 8 weeks, 86 patients were randomly assigned to treatment group and control group, each group with 43 patients, the treatment group continued medicine for 4 weeks, the control group without any treatment.The two groups of patients FENO measurement values, cough symptom score before treatment and after treatment for 8 weeks, 12 weeks were compared. Results After treatment for 8 weeks, cough symptom scores and FeNO in the two groups values decreased significantly compared with those before treatment(P<0.05), 12 weeks after treatment, treatment group cough product, FeNO values, were lower than in control group(P<0.05).Conclusion Fluticasone salmeterol powder inhalation can effectively improve the symptoms of cough cough variant asthma.Symptom score and FENO value can be used as a monitoring index to evaluate the therapeutic effect of CVA, and the FENO value is more effective, which has the advantages of non-invasive, simple, safe, rapid and repeatable.

Ocular surface diseases are common diseases in ophthalmology.It is difficult for clinicians to make early and accurate diagnosis based on symptoms, signs and serologic examination.Compared with the blood test, the tear test can reflect the condition of the local ocular surface and it has the characteristics of being non-invasive, having high diagnostic accuracy and fast diagnostic speed.Tear detection uses modern detection techniques such as polymerase chain reaction, enzyme-linked immunosorbent assay, gene chip and protein chip to detect the pathogen and immune components in tear, including microbial nucleic acid, tear antibody (IgM, IgG, IgE, IgA, antinuclear antibody, etc.) and cytokines (interleukin, tumor necrosis factor, interference factor, transforming growth factor, epidermal growth factor, etc.). Tear detection provides information about the pathogen infection and immune response on the ocular surface.At present, a large number of domestic and foreign tear detection studies show that there are certain regular changes in tear composition, not only in ocular surface diseases, uveitis, fundus diseases, thyroid related ophthalmopathy, but even in systemic diseases such as diabetes, hepatitis B, acquired immune deficiency syndrome etc.In addition, the research of tear pathogen and immune components is of great significance to study the cause, biochemical and immune process, treatment of diseases.In this paper, the latest research progress of tear collection, the clinical significance of tear pathogen and immune components and the characteristics of tear in various diseases was summarized and analyzed to provide reference for clinical practice.

Child ; Desensitization, Immunologic ; Humans ; Rhinitis, Allergic ; Rhinitis, Allergic, Perennial ; therapy

Air Pollutants ; analysis ; Dust ; analysis ; Environment Design ; Environmental Monitoring ; instrumentation

Objective: To explore the protective effect of minocycline on hepatic ischemia-reperfusion injury (IRI) in rats and the underlying mechanisms. Methods: A total of 54 male Sprague-Dawley rats were randomly divided into 3 groups: the sham-operated group (control group), the ischemic–reperfusion (IR group), and the minocycline preconditioning group (n=18 per group). The rats in the minocycline preconditioning group were given minocycline (45 mg/kg) by gastric irrigation at 36 h before operation and then were subsequently administered with minocycline (22.5 mg/kg) at every 12 h. hTe rats were sacriifcedat 2, 6, 24 h after reperfusion. The serum levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST) and lactate dehydrogenase (LDH) were measured. HE staining of liver tissues was performed to detect the histological changes, and the degree of liver IRI according to Suzuki score were calculated. hTe levels of malondialdehyde (MDA) and myeloperoxidase (MPO) were determined by spectrophotometer; the mRNA expression of tumor necrosis factor-α (TNF-α) and interleukin-1 beta (IL-1β) in the liver were measured by real-time PCR; Dickkopf-1 (DKK-1) and beta-catenin (β-catenin) protein expression in the liver were detected by Western blot. Results: Atfer 2, 6, 24 h reperfusion, compared with the IR group, the liver function (ALT, AST and LDH) in the minocycline group was signiifcantly improved (allP<0.05); the Suzuki’s scores and the levels of hepatic TNF-α and IL-1β mRNA were signiifcantly decreased (allP<0. 05); the MDA and MPO levels the liver were decreased (bothP<0.05); the protein expression of hepatic DKK-1 was decreased (P<0.05), while the protein expression of β-catenin was increased (P<0.05). Conclusion: Minocycline can alleviate the ischemic-reperfusion injury mainly through reducing oxidative stress and inhibiting the release of pro-inlfammatory cytokines depends on the activation of the Wnt/β-catenin signaling pathway in the liver.

OBJECTIVE To compare the results of three kinds of Candida albicans biofilm model systems in vitro,and observe the dynamic course of C.albicans biofilm formation.METHODS C.albicans model strain ATCC 90038 formed biofilm structure on 96 well microtiter plates,on the glass plates in 6 well cell culture plates,and on the continuous culture systems-chemostat,which was observed by fluorescent microscope,and by XTT reduction method to quantitatively analyze the formation of biofilm.RESULTS All of the three kinds of biofilm model systems could form mature biofilm structure,and the quantitative analysis of biofilm formation indicated that three methods were significantly correlated.CONCLUSIONS These three model systems are all ideal methods for studying the C.albicans biofilm,we should choose the appropriate method according to objective of the study.

Objective:To understand the liability situation of urban public hospitals in Jiangxi.Methods:Qualitative and quantitative analysis,descriptive analysis and comparative analysis were used to analyze the causes and changes of urban public hospitals' debt increase.Results:To meet the increasing demand for health diagnosis and services,debt infrastructure and the purchase of equipment coupled with inadequate financial investment were performed in public hospitals,which resulted in the sustainably increasing of liabilities level in urban public hospitals.Conclusion:Governments at all levels should increase the capital expenditure of hospital construction and equipment investment,strictly control the public hospital debt behavior,the implementation of the reform of public hospitals canceled drug addition,establishing the characteristics of the medical industry personnel salary system and other measures to resolve the synchronous speed of public hospital debt and prompt public hospital debt risk control in the reasonable scope.

Objective To evaluate the clinical effects of different doses of brachical plexus anesthesia in the emergency suture of hand trauma in preschool children .Methods Forty preschool children with hand trauma were choosen ,who were undergoing e‐mergency suture by general anesthesia combined with brachical plexus anesthesia .They were divided into group A(1 mg/kg ,n=20) and group B(2 mg/kg ,n=20) ,body movement during skin incision ,local injection with lidocaine ,propofol dose ,surgical time and wake up time were compared .Results There was no significant differences between the two groups in surgical time(P>0 .05) ,the cases of body movement during skin incision and local injection with lidocaine were 8 children ,which was higher than 2 children in group B(all P<0 .01) ,ropivacaine dose(14 .5 ± 5 .5)mg in group A were lower than group B (31 .6 ± 7 .2)mg(P<0 .01) ,propofol dose in group A(75 .6 ± 15 .2)mg were higher than group B(32 .7 ± 8 .6)mg(P<0 .01) ,wake up time in group A(11 .2 ± 3 .3)min were longer than group B(5 .9 ± 2 .3)min (P< 0 .01) .Conclusion The clinical effect of ropivacaine 2 mg/kg was superior than 1 mg/kg in brachical plexus anesthesia .

Objective To investigate the possible role and mechanism of the neurotoxic effect of dexamethasone on adult rats after having focal cerebral ischemia. Methods The rat model of focal cerebral ischemia was established by permanent middle cerebral artery occlusion (MCAO) One hour after ishemia,the experimental groups were treated with dexamethasone (5 mg/kg) while the control groups were treated with saline TUNEL staining and In-suit RT-PCR were used to show the changes of apoptosis and the expression of Fas mRNA at ipsilateral cerebral hemisphere. Results TUNEL positive cells were present in a time from 48 h to 72 h, localizing at peripheral ischemic area The expression of Fas mRNA at peripheral ischemic area in control groups began at 12 h, peaked at 24 h, and decreased to a lower level at 48 h and 72 h, and returned to the baseline at 120 h Treatment with dexamethasone after ischemia made apoptosis present at 24 h and the number of TUNEL positive cells at 48 h exceeded that in the control group at 48 h ( P