1.Chronic Myelocytic Leukemia.
Korean Journal of Pediatrics 2004;47(Suppl 2):S367-S383
No abstract available.
Leukemia, Myelogenous, Chronic, BCR-ABL Positive*
2.A Case of Neonatal Diabetes Mellitus: Transient or Permanent?.
Dae Yeol LEE ; Ho Keun YI ; Pyoung Han HWANG
Journal of Korean Society of Pediatric Endocrinology 2000;5(2):210-214
A case of neonatal diabetes mellitus is described. The child presented with low birth weight but was normal in appearance. She was acidotic and ketonuria was observed. The HLA typing was DR1 and 3, and insulin autoantibodies were negative. Genetic analysis with polymorphic DNA markers for chromosome 6 indicated biparental inheritance. She required insulin therapy for the control of hyperglycemia, and insulin dependence continues after 8 months of age.
Autoantibodies
;
Child
;
Chromosomes, Human, Pair 6
;
Diabetes Mellitus*
;
Genetic Markers
;
Histocompatibility Testing
;
Humans
;
Hyperglycemia
;
Infant, Low Birth Weight
;
Infant, Newborn
;
Insulin
;
Ketosis
;
Wills
3.Inhibition of L-type calcium currents by magnesium sulfate on the rat basilar artery smooth muscle cells
Naveen Sharma ; Janardhan Prasad Bhattarai ; Pyoung Han Hwang ; Seong Kyu Han ; Yun Su Yang
Neurology Asia 2014;19(3):301-307
Objective: Vasospasm remains the leading cause of cerebral damage after aneurysmal subarachnoid
hemorrhage. Although magnesium regulates the calcium influx in vascular smooth muscle and
endothelial cells, it has not been reported whether L-type calcium channels are involved in magnesiuminduced
vascular relaxation in rat basilar artery. So, the effect of magnesium sulfate on L-type calcium
currents in freshly isolated smooth muscle cells from rat basilar artery was investigated. Methods:
The smooth muscle cells were isolated from rabbit basilar artery by enzyme treatment. L-type Ca2+
currents were identified using cesium chloride, a potassium channel blocker and Bay K8644, an
activator of L-type Ca2+ channel. Currents were recorded under step pulse whole cell patch clamp
technique. Results: In the presence of cesium chloride (in pipette solution), inward currents were
observed by depolarizing step pulses. The inward currents were significantly reduced by nimodipine
(n=4, p<0.05), an L-type Ca2+ channel blocker and increased by Bay K8644 (n=5, p<0.05), an L-type
Ca2+ channel activator. The L-type calcium currents (156±17.0 pA, n=12) were significantly reduced
by the application of 5 mM magnesium sulfate (53.8±7.0 pA, n=12, p<0.01).
Conclusion: These results suggest that magnesium may relax cerebral vessel of rat basilar artery
through decreasing intracellular Ca2+ ion by inhibition of L-type Ca2+ channels.
4.Comparison of anticancer drug efficacy using the short-term microplate culture and MTT dye reduction assay.
Nam Ki KIM ; Kwang Rae LEE ; Pyoung Han HWANG ; Jung Soo KIM
Journal of the Korean Pediatric Society 1993;36(10):1426-1433
Individual tumors, even those of the same histologic type, show varying sensitivity to specific cytotoxic agent. Therefore, sensitivity testing assume an increasingly important as an orientational aid in planning chemotherapy. In the past decade there have been many attempts to develop a chemosensitivity test that would predict the clinical effectiveness of various chemostherapeutic agents against human neoplasms. In the United States National Institue's anticancer drug screening program, a colorimetric assey based on the ability of live cells to reduce a tetrazolium-base compound(MTT) to a blue formazan product was used. There has been an increase in reports of a chemosensitivity assay that use tetrazolium dyes and current the assay is in use in our country. The efficacy of several anticancer drug (vincristine sulfate, Etoposide, doxorubicin CDDP) were evaluated using the in vitro chemosensitivity of MTT assay with two cancer cell lines (MOLT-4, KHOS/NP). The follows obtained. 1) CI50 on MOLT-4 are 0.55ng/ml and 0.81ng/ml for vincristine and oncovin, 142.30ng/ml and 78.75ng/ml for lastet and vepesid, and 19.75ng/ml, 20.43ng/ml and 8.66ng/ml for ADR, ADM and adriblastin, respectively. 2) CI50 on KHOS/NP are 691.35ng/ml, 873.73ng/ml, 1,205.22ng/ml, 768.81ng/ml and 672.19ng/ml for cisplan, cisplatin, cispatin, platinol and cisplatin G, and 9.22ng/ml, 11.46ng/ml and 4.28ng/ml for ADR, ADM and adriblastin, respectively. In conclusion the MTT dye reduction assay to anticancer drug sensitivity using short-term microplate culture might serve as a reliable tool for the selection of effective chemotherapeutic agents in patients with cancers.
Cell Line
;
Cisplatin
;
Coloring Agents
;
Doxorubicin
;
Drug Evaluation, Preclinical
;
Drug Therapy
;
Etoposide
;
Humans
;
United States
;
Vincristine
5.A case of tuberous sclerosis developing with subependymal giant-cell astrocytoma.
Young Chul AHN ; Yong Suk CHOI ; Pyoung Han HWANG ; Jung Soo KIM
Journal of the Korean Pediatric Society 1991;34(7):1027-1033
No abstract available.
Astrocytoma*
;
Tuberous Sclerosis*
6.Changes of igG subclasses in the sera of the children with Kawasaki disease.
Seog Beom CHO ; Sun Kyu PARK ; Pyoung Han HWANG ; Jung Soo KIM ; Sa Hyoung CHOI
Journal of the Korean Pediatric Society 1993;36(9):1197-1202
Kawasaki disease is an acute vasculitis of infancy and early childhood characterized by high fever, rash, mucositis, lymphadenopathy and coronary artery damage. The failure to indentify a causative organism using convetional culture and serological techniques, and the lack of response to antibiotics indicate that the disorder is probably not due to any known bacterial or viral pathogens. During the acute phase of the disease, the alterations of T and B cell functions, changes of cytokine and immunoglobulin levels have been reported. This study was performed to investigate the changes of immunoglobulins levels in patients with Kawasaki disease. IgG, IgA, IgM and IgG subclasses were measured using immunoprecipitation and EIA in the sera of patients with Kawasaki disease. The results were as follows: 1) Acute phase reactants such as CRP and ESR were significantly increased in Kawasaki patients compared to those in control patients(p<0.01). 2) Serum IgG levels in Kawasaki disease were markedly increased than those in control patients, while serum IgA and IgM levels showed no significant changes (P: No Significance). 3) IgG1 and IgG4 were predominantly increased increased in the sera of Kawasaki patients, while IgG2 and IgG3 were not significantly increased (P: No Significance). With these results, unidentified infectious organism with abnormal immune response could be suggested as an etiologic factor of Kawasaki disease.
Acute-Phase Proteins
;
Anti-Bacterial Agents
;
Child*
;
Coronary Vessels
;
Exanthema
;
Fever
;
Humans
;
Immunoglobulin A
;
Immunoglobulin G*
;
Immunoglobulin M
;
Immunoglobulins
;
Immunoprecipitation
;
Lymphatic Diseases
;
Mucocutaneous Lymph Node Syndrome*
;
Mucositis
;
Vasculitis
7.Osteogenic Sarcoma as Second Malignant Neoplasm After Treatment of Retinoblastoma.
Ja Young KIM ; Moon Whan LEE ; Ju Hyung KIM ; Pyoung Han HWANG ; Jung Soo KIM
Journal of the Korean Pediatric Society 1990;33(12):1735-1740
No abstract available.
Osteosarcoma*
;
Retinoblastoma*
8.Changes of PPD-Specific Immunoglobulin in Pulmonary Tuberculosis After Treatment.
Ja Young KIM ; Mun Hwan LEE ; Pyoung Han HWANG ; Chan Uhng JOO ; Jung Soo KIM
Journal of the Korean Pediatric Society 1990;33(12):1647-1655
No abstract available.
Immunoglobulins*
;
Tuberculosis, Pulmonary*
9.Changes of beta-lactoglobulin Specific Immunoglobulins According to the Age and the Onset of Cow's Milk Feeding.
Jong San LEE ; Soo Young CHO ; Pyoung Han HWANG ; Soo Cheol CHO ; Jung Soo KIM
Journal of the Korean Pediatric Society 1990;33(12):1639-1646
No abstract available.
Immunoglobulins*
;
Lactoglobulins*
;
Milk*
10.Changes of Plasma Inactive Renin in Newborn Infant and Children.
Pyoung Han HWANG ; Woong Kyou PARK ; Heon Sook LEE ; Jung Soo KIM ; Kyung Woo CHO
Journal of the Korean Pediatric Society 1984;27(9):872-876
No abstract available.
Child*
;
Humans
;
Infant, Newborn*
;
Plasma*
;
Renin*