BackgroundThe cardiovascular disease is leading cause of mortality and constantly increasing every year forpast decades. In 2014, cardiovascular diseases were the leading cause of NCD deaths (17.5 milliondeaths) [7] In Mongolia cardiovascular disease is also leading the cause of mortality and morbidity.It is increasing every year, 479.4 per 10000 population in 2012 was increased to 848.1 per 10000population in 2013 [1, 2].Dr.Jerardin, prof. Z.Lkhagvasuren implanted first stent in Mongolia in 2000. There is rapiddevelopment in interventional cardiology treatment since then.Material and MethodsSince 2011 until 2015, total of 4545 coronary angiography, 2322 stent implantation was performed.This study has included the patients of last 5 years. Total of 1257 patients who were treated with AMIduring 2011 to 2015 at the Third central State Hospital were analyzed.Results73.7% of the patients had left coronary artery disease and 26.3% had right coronary artery disease.Tis findings were similar with other local researcher’s study [3, 4].59.4% of the occlusions were 75-99% stenosis, 23.5% had total occlusion. 62.9% of LAD occlusionwere stenosis over 75%. It demonstrated majority of cases performed were high severe lesions. Thisalso may be demonstrating that patients are receiving treatment in their late stage of the disease.Every stage complication such as mortality, was 3.5%, complication such as restenosis followingone month of after stent implantation was 9-13.1%. Overall mortality rate has decreased each year,11.9% in 2011, 7.8% in 2012, and 4.6% in 2013 relatively. General achievement is now 88.1-95.4%,which is comparable to other countries.Conclusion1257 patients presented with acute myocardial infarction were studied: 880 (70, 0%) were male, 377(30, 0%) were female. Mean age of patients was 55, 08±1, 23; it was 75 patients had no risk factors,751 patients with 1-2 risk factors, 431 patients with 3 or more risk factors. Angiographic distributionof lesion shows 74, 4% had LAD disease, 64, 5% had RCA disease, 25, 8% had LCX lesion, 12, 9%had dialonal LCA lesion.

Background:The cardiovascular disease especially coronary artery disease is the leading cause of mortality in worldwide. There is lack of research study which evaluated stenosis of coronary atherosclerosis. It is known that coronary stenosis is highly connected to the levels of biomarkers. Coronary atherosclerosis correlated with endothelin receptor type A (EDNRA) levels in a group of patients suspected of having coronary artery disease.Objective:The goal of this study was to evaluate the relation between the coronary atherosclerosis and levels of EDNRAMethods:A total of 311 participants were involved in this study. A case-control study was used in the study. The baselines data were collected from the department of Angiography at the National Third Central Hospital and National lilood Transfusion and Research center. We have determined the degree of coronary atherosclerosis using the Angiography machine and Elisa were used for detecting the blood endothelin levels in all groups.Results:98 participants were diagnosed with stenosis and occlusion. The blood endothelin levels were estimated to 6.32±0.64 pg/ml which refer to () degree of coronary stenosis, the first degree of stenosis of coronary atherosclerosis is estimated to 5.56±0.22pg/ml, the second degree of stenosis of coronary atherosclerosis is estimated to 5.42J0.34 pg/ml, the third degree of stenosis of coronary atherosclerosis is estimated to 5.87 H). 13 pg/ml, the fourth degree of stenosis of coronary atherosclerosis is I'SlilllSltll lo 5.69±0.09 pg/ml, it was estimated to 5.88±0.13 pg/ml in control groups. Level of EDNRA (occupying one segment of coronary artery) was estimated to 5.77±0.08 pg/ml. two segment involvement was estimated to 5.72±0.16 pg/ml, three segment involvement 5.73±0.19 pg/ml. four segments involvement 5.50±0.25 pg/ml, respectively.Conclusion:The blood endothelin level and coronary artery stenosis were not correlated statistically significant in control group of the study. However, blood endothelin levels were increased in patients who tend to experience the coronary artery stenosis.

BACKGROUND Bovine colostrums is the milk secreted by cows during the first few days after parturition. It contains many essential nutrients and bioactive components, including growth factors, immunoglobulins, lactoperoxidase, lactoferrin and cytokines ets. Lactoferrin has been reported for its multifunctional properties such as antifungal, antibacterial, antiviral antioxidant and anticancer activities. The aims of this study focused on the isolation and purification of lactoferrin from Mongolian bovine colostrums. Lactoferrin purified using HiTrap DEAE an ion exchange chromatography. Lactoferrin purification efficiency was about 60.5%. The single band of purified lactoferrin has been observed in SDS-PAGE electrophoresis. METHODS Bovine colostrum was collected at a cow farm in the Darkhan province of Mongolia. At first the cream was separated by centrifugation (10000 xg 20 min at 4oC). In order to separate the whey, the samples were precipitated with 1mol/l to pH 4.6 and centrifuged at 10000 g 20 min again. The samples of whey were stored at -18oC to the analysis. Lactoferrin was purified by HiTrap DEAE an ion exchange chromatography using 0.005 M phosphate buffer (pH 7.7) and linear gradient NaCl from 0.25M, 0.5M, 1M. During chromatography, protein in the eluents was monitored by ultraviolet absorbation at 280 nm with the instrument. Purity test done by using polyacrylamide gel electrophoresis under denaturated condition (SDS-PAGE) method by Laemmli (1970). For HPLC determination of the lactoferrin by Shimadzu Nexera X2 HPLC system with UV/ VIS detector were used. Detection was carried out at the wavelength 280 nm. Separation was performed on a chromatographic column Protein R C18 ,2.2 x 150 mm, 5 μm particle size. Linear gradient and flow rate 0.2 ml/min were used. Mobile phase a consisted of water / acetonitrile/ trifluoroacetic acid ( 95:5:0.1). The column temperature was set at 40oC and injection volume was 10 μl. Data were collected and evaluated by software Lab Solution. An external standard method for quantification analytes was used. RESULTS Purified lactoferrin in the present study had a good concentration and purification efficiency was about 60.5 %. Protein fraction from 1M NaCl gradient delivers sharp and clean peak to HPLC chromatogram that fits intensity and retention time of standard bovine lactoferrin. Ammount of lactoferrin in bovine colostrums was 0.6 mg/ml and it`s molecular weight 80 kDa as a standard sample. The retention time of lactoferrin fraction which is purified by SDS-PAGE gel electrophoresis. The peak of fraction same compared to the standard lactoferrin 5.8 minutes by HPLC analysis. CONCLUSION Ion exchange chromatography shows reliable and easy isolation of lactoferrin from Mongol bovine colostrum.