Objective To observe the effect of Pirarubicin (THP) with other chemical agents in treating hematopoietic Malignancies. Methods 90 inpatients diagnosed as hematopoietic malignancies between January 2000 to December 2005 were treated with THP with other chemical agents. 30 had not been treated previously ,(untreated) while 60 were relapsing or resistant to previous treatment. Acute leukemia(AL) was 60 cases, Malignancy lymphoma(ML) was 21 cases, Multiple myeloma(MM) was 9 cases. Results The complete response(CR) rate among AL untreated and relapsing/resistant groups were 91.7% and 38.5%; 71.5% of response rate were malignancy Lymphoma(ML); 67.8% of response rate ware multiple myeloma(MM).The total response rate was 80.0%. The main side-effect of THP is bone marrow depression which led to fever and bleeding with rates 30.2%, 20.5%. The non-bone marrow side effects were tolerable. Damage to the liver was a prominent occurrence 20.8%. Conclusions THP was effective as the first line anti-tumor drug with other chemical agents in treating hematopoietic malignancies, its side-effects were mild and well tolerable.

Objective To estimate the effect of sodium arsenite(AS2O3)in treating adult chronic myeloid leukemia(CML).Methods 30 inpatients diagnosed as CML from December 2002 to January 2008 were treated with AS2O3,of which 16 cases had not been treated previously,while 14 cases were relapsing or resistant to previous treatment.Results The complete response rate was 63.3%,while the partial response rate was 20.0%,non-response rate was 16.7%.The total response rate was 83.3%.Conclusion AS2O3 is an effective drug in treating CML with side effects being mild and well tolerance.

Objective To investigate myostatin gene mRNA expression in the muscle tissue from patients with muscle weakness suffering from different illness.Methods The clinical data of our patients were all from the Muscular Disease Center,Department of Neurology,People' s Liberation Army General Hospital.A total of 75 patients suffered from muscular weakness were included consecutively.Skeletal muscle biopsies were obtained with informed consent from all 75 patients.The diagnosis was confirmed by two senior doctors for muscular disease according to the clinical feature,the results of electromyography,serum creatine kinase activity and histopathological examination.Among of them,21 cases were diagnosed as polymyositis,15 cases progressive muscular dystrophy,5 cases neurogenic amyotrophy,4 cases chronic muscle fiber damage,4 cases mitochondrial myopathy,4 cases lipid storage myopathy,4 cases myotonic dystrophy,3 cases muscular dystrophy in adults,2 cases dermatomyositis,and 2 cases inclusion body myositis.There were 2 cases characterized by pure high activity of creatine kinase.And the other 9 cases were diagnosed as non-neuromuscular disease.The expression of myostatin gene mRNA in muscle tissue was evaluated by reverse transcription polymerase chain reaction method,with glyceraldehyde-3-phosphate dehydrogenase as internal reference.Results The expression of myostatin gene mRNA was detected in 63 patients,but not in other 12 cases,and the percentage of positive expression was 84％.The expression index was with great variation,from 0 to 3.52.In positive cohort,the index was correlated positively with the duration of disease (r =0.236,P =0.041).The activities of creatine kinase in positive expression cohort were higher than that of negative one,but nonsignificantly.Conclusion The expression of myostatin gene mRNA in muscle tissue may not correlate to the entity of atrophic muscular disease because of its great variation.

Aim To study the neuroprotective effect of Acteoside against MPTP-induced mouse model of Parkinsons disease (PD) and its mechanism. Methods The behavioral testing of C57 mice was assessed using spontaneous movement and rotarod test. DA levels in striatum were measured using HPLC-EC. Dopaminergic neurons in the substantia nigra were observed by immunohistochemical staining with an anti-Tyroxine hydroxylase (TH) antibody. Moreover, the mechanism of the neuroprotective effect of acteoside was investigated using Western blot analysis with an anti-?-synuclein antibody in the substantia nigra and striatum. Results ① Compared with control, MPTP lesion significantly reduced the number of spontaneous movement and latent period of mice on the rotating rod (both P

Recently,the techniques for proteomics have made remarkable progress.They are widely used in various fields of life sciences,providing strong technical supports for relevant researches.Especially,proteomics technology has made prominent contributions to drug development and mechanism studies,magnificently improving the efficiency of discovering new drugs.This paper summarizes the classical method and new technology of proteomics.Also,its applications in drug study,including targets screening,mechanism studies,drug toxicology,researches of resistance mechanisms and clinical medical studies have been reviewed.

Objective To investigate the expression levels and the roles of induced nitric oxide synthase(iNOS) in rats′ kidney after interruption of blood flow and the following ischemia-reperfusion(IR)injury.Methods Rats were divided into 3 groups:interruptions(5,15,30 min),IR(2,5,8,24,48,72 h),control groups.They were killed at different time points.Kidney tissue samples were studied by immunohistochemical method.Results iNOS increased significantly(P

Objective To assess the value of outer thimble of epidural anesthesia needle in laparoscopic high ligation for indirect inguinal hernia in children.Methods From April 2003 to May 2006,outer thimble of epidural anesthesia needle,instead of sledge-like hook needle,was utilized in 230 cases during laparoscopic high ligation for indirect inguinal hernia in chilren.Results All the operations on 230 patients were successfully performed.The operation time was 8-10 minutes in 161 patients with unilateral hernia,15-20 minutes in 39 patients with incarcerated hernia,16-20 minutes in 30 patients with bilateral hernia.Edema of scrotum,testis pain and ischemia were not found after operation.Follow-up for 2-6 months in 111 cases,6-12 months in 52 cases,12-24 months in 37 cases,24-36 months in 30 cases showed no recurrence.Conclusions Outer thimble of epidural anesthesia needle can be substituted for sledge-like hook needle in children hernia high ligation,with the advantages of simple procedure and economy.

RNAi is an efficient antiviral system, and viral gene-specific siRNAs are very promising antiviral inhibitors. However, many viruses have evolved highly sophisticated mechanisms that interfere with both siRNA- and miRNA-guided silencing pathways. Deeper understanding the strategies exploited by viruses provides the basis for the development of effective RNAi-based therapies that prevent viral evading. Therefore, the latest progress on the strategies exploited by viruses for evading the RNAi is reviewed.

Objective To investigate the prevalence of Kaschin-Beck disease in Wulan and Dulan counties,and to provide basic data in distribution of Kashin-Beck disease.Method In 2015,according to the Diagnosis of Kaschin-Beck Disease (WS/T 207-2010),in 18 villages of Wulan (Tongpu Town,Xiligou Town) and Dulan (Xiariha Town,Xiangride Town) counties,children aged 7-15 and adults over 16 years old who had bone and joint dysfunction underwent epidemiological,clinic and X-ray examination.Results A total of 3 800 children were examined,6 children were found with clinic syndrome of degree Ⅰ Kaschin-Beck disease,the detection rate was 0.16％.By X-ray examination,one student with Kaschin-Beck disease was from other place;other 5 students' X-ray signs were normal.A total of 222 adults older than 16 years old who had bone and joint dysfunction were examined,none of them was found with clinic syndrome of degree Ⅰ Kaschin-Beck disease,the detection rate was 0.Conclusion There is no patient with Kaschin-Beck disease in Wulan and Dulan counties,Qinghai Province.

The regularity and the preventive and curative measures about the post-operative infections in 3 orthotopic cardiac transplant recipients were reviewed.The results showed that the bacterial infections and the opportunistic fungal infections were common within 1～2 months after the cardiac transplantation.The sensitive antibiotics could completely control the infections.More than 3 months after surgery,the infections were gradually reduced.It was suggested that the prevention and treatment of the post-operative infections should be reinforced in the early stage of transplantation.