Objective To observe the effect of Pirarubicin (THP) with other chemical agents in treating hematopoietic Malignancies. Methods 90 inpatients diagnosed as hematopoietic malignancies between January 2000 to December 2005 were treated with THP with other chemical agents. 30 had not been treated previously ,(untreated) while 60 were relapsing or resistant to previous treatment. Acute leukemia(AL) was 60 cases, Malignancy lymphoma(ML) was 21 cases, Multiple myeloma(MM) was 9 cases. Results The complete response(CR) rate among AL untreated and relapsing/resistant groups were 91.7% and 38.5%; 71.5% of response rate were malignancy Lymphoma(ML); 67.8% of response rate ware multiple myeloma(MM).The total response rate was 80.0%. The main side-effect of THP is bone marrow depression which led to fever and bleeding with rates 30.2%, 20.5%. The non-bone marrow side effects were tolerable. Damage to the liver was a prominent occurrence 20.8%. Conclusions THP was effective as the first line anti-tumor drug with other chemical agents in treating hematopoietic malignancies, its side-effects were mild and well tolerable.

Objective To estimate the effect of sodium arsenite(AS2O3)in treating adult chronic myeloid leukemia(CML).Methods 30 inpatients diagnosed as CML from December 2002 to January 2008 were treated with AS2O3,of which 16 cases had not been treated previously,while 14 cases were relapsing or resistant to previous treatment.Results The complete response rate was 63.3%,while the partial response rate was 20.0%,non-response rate was 16.7%.The total response rate was 83.3%.Conclusion AS2O3 is an effective drug in treating CML with side effects being mild and well tolerance.

Objective To investigate myostatin gene mRNA expression in the muscle tissue from patients with muscle weakness suffering from different illness.Methods The clinical data of our patients were all from the Muscular Disease Center,Department of Neurology,People' s Liberation Army General Hospital.A total of 75 patients suffered from muscular weakness were included consecutively.Skeletal muscle biopsies were obtained with informed consent from all 75 patients.The diagnosis was confirmed by two senior doctors for muscular disease according to the clinical feature,the results of electromyography,serum creatine kinase activity and histopathological examination.Among of them,21 cases were diagnosed as polymyositis,15 cases progressive muscular dystrophy,5 cases neurogenic amyotrophy,4 cases chronic muscle fiber damage,4 cases mitochondrial myopathy,4 cases lipid storage myopathy,4 cases myotonic dystrophy,3 cases muscular dystrophy in adults,2 cases dermatomyositis,and 2 cases inclusion body myositis.There were 2 cases characterized by pure high activity of creatine kinase.And the other 9 cases were diagnosed as non-neuromuscular disease.The expression of myostatin gene mRNA in muscle tissue was evaluated by reverse transcription polymerase chain reaction method,with glyceraldehyde-3-phosphate dehydrogenase as internal reference.Results The expression of myostatin gene mRNA was detected in 63 patients,but not in other 12 cases,and the percentage of positive expression was 84％.The expression index was with great variation,from 0 to 3.52.In positive cohort,the index was correlated positively with the duration of disease (r =0.236,P =0.041).The activities of creatine kinase in positive expression cohort were higher than that of negative one,but nonsignificantly.Conclusion The expression of myostatin gene mRNA in muscle tissue may not correlate to the entity of atrophic muscular disease because of its great variation.

Aim To study the neuroprotective effect of Acteoside against MPTP-induced mouse model of Parkinsons disease (PD) and its mechanism. Methods The behavioral testing of C57 mice was assessed using spontaneous movement and rotarod test. DA levels in striatum were measured using HPLC-EC. Dopaminergic neurons in the substantia nigra were observed by immunohistochemical staining with an anti-Tyroxine hydroxylase (TH) antibody. Moreover, the mechanism of the neuroprotective effect of acteoside was investigated using Western blot analysis with an anti-?-synuclein antibody in the substantia nigra and striatum. Results ① Compared with control, MPTP lesion significantly reduced the number of spontaneous movement and latent period of mice on the rotating rod (both P

Recently,the techniques for proteomics have made remarkable progress.They are widely used in various fields of life sciences,providing strong technical supports for relevant researches.Especially,proteomics technology has made prominent contributions to drug development and mechanism studies,magnificently improving the efficiency of discovering new drugs.This paper summarizes the classical method and new technology of proteomics.Also,its applications in drug study,including targets screening,mechanism studies,drug toxicology,researches of resistance mechanisms and clinical medical studies have been reviewed.

Objective To investigate the expression levels and the roles of induced nitric oxide synthase(iNOS) in rats′ kidney after interruption of blood flow and the following ischemia-reperfusion(IR)injury.Methods Rats were divided into 3 groups:interruptions(5,15,30 min),IR(2,5,8,24,48,72 h),control groups.They were killed at different time points.Kidney tissue samples were studied by immunohistochemical method.Results iNOS increased significantly(P

Aged ; Catheter Ablation ; adverse effects ; Echocardiography ; Heart Atria ; Humans ; Male ; Postoperative Complications ; diagnostic imaging ; Tachycardia, Supraventricular ; diagnostic imaging ; surgery ; Thrombosis ; etiology

Objective To observe the effects of early enriched environment intervention on the expression of growth-associated protein-43(GAP-43) in the hippocampus of rats with hypoxic-ischemic brain injury (HIBI). Methods After the establishment of HIBI model in SD rats by the method of Rice, the animals were divided randomly into 2 groups: the intervention group and non-intervention group. The sham-operation rats were used as control group. Enriched environment intervention had been administrated to the rats of intervention group since the 2nd day after HIBI. On the 3rd, 7th, 14th, 21st and 28th day, immunohistochemistry and RT-PCR were used to measure the expressions of GAP-43 protein and mRNA in the hippocampus of rats. Results The expression of GAP-43 protein and mRNA in the rat hippocampus were increased in the non-intervention group than in the control group(P

Objective To observe the effects of environmental stimulation at different stages of development on postnatal rats with hypoxia-ischemic brain injury (HIBI). Methods HIBI postnatal SD rats models were established by dissecting and ligating the left common carotid artery, and after 2 h of recovery, exposing to 8% oxygen-92% nitrogen gas mixture for 2 h. The HIBI rats were randomly divided into early intervention groups, late intervention group, non-intervention group. Intervention group were kept in enriched environment before or after weaning for 20 d. Non-intervention group and control group were kept in a standard environment. Space learning and memory were tested with Morris water maze. Results The ability of space learning and memory in early intervention group was better than that in late intervention group (P

Objective To observe the expression of growth-associated protein-43(GAP-43) in the hippocampus of neonatal rats with hypoxic-ischemic brain damage (HIBD). Methods HIBD was established by the method of Rice in 48 SD rats aged 7 days and another 48 matched normal rats served as control. Immunohistochemistry and RT-PCR were respectively used to detect the expression of GAP-43 protein and mRNA in all rat hippocampus on day 8, 10, 14, 21, 28, 35 after HIBD (n=8 at each time point for each group). Results The expression of GAP-43 protein and mRNA in hippocampus was obviously increased in HIBD rats as compared with normal rats, the peak of GAP-43 protein appeared at 3rd week, and the peak of mRNA at 2nd week. Conclusion The expression of GAP-43 protein and mRNA increased in the hippocampus of rats following HIBD, which was possibly related to the recovery of injured hippocampus.