1.Curcumin alters motor coordination but not total number of Purkinje cells in the cerebellum of adolescent male Wistar rats.
Ginus PARTADIREDJA ; Sutarman ; Taufik Nur YAHYA ; Christiana Tri NURYANA ; Rina SUSILOWATI
Journal of Integrative Medicine 2013;11(1):32-38
OBJECTIVEThe present study aimed at investigating the effects of curcumin on the motor coordination and the estimate of the total number of cerebellar Purkinje cells of adolescent Wistar rats exposed to ethanol.
METHODSThe total of 21 male Wistar rats aged 37 d old were divided into three groups, namely ethanol, ethanol-curcumin, and control groups. The ethanol group received 1.5 g/kg ethanol injected intraperitoneally and water given per oral; the ethanol-curcumin group received 1.5 g/kg ethanol injected intraperitoneally and curcumin extract given per oral; the control group received saline injection and oral water. The treatment was carried out daily for one month, after which the motor coordination performance of the rats was examined using revolving drum apparatus at test days 1, 8, and 15. The rats were finally sacrificed and the cerebellum of the rats was further processed for stereological analysis. The estimate of the total number of Purkinje cells was calculated using physical fractionator method.
RESULTSThe ethanol-curcumin group performed better than both ethanol and control groups in the motor coordination ability at day 8 of testing (P< 0.01). No Purkinje cell loss was observed as a result of one month intraperitoneal injection of ethanol.
CONCLUSIONCurcumin may exert beneficial effects on the motor coordination of adolescent rats exposed to ethanol via undetermined hormetic mechanisms.
Animals ; Cell Count ; Cerebellum ; cytology ; drug effects ; Curcumin ; pharmacology ; Ethanol ; toxicity ; Male ; Motor Activity ; drug effects ; Psychomotor Performance ; Purkinje Cells ; cytology ; drug effects ; Rats ; Rats, Wistar
2.The influence of estazolam on human neurobehavioral function.
Journal of Forensic Medicine 2010;26(1):30-32
OBJECTIVE:
To study the relation between human blood estazolam concentration and neurobehavioral function.
METHODS:
The neurobehavioral ability of 10 volunteers were measured with computer-administered neurobehavioral evaluation system-chinese3 (NES-C3) and SMART EquiTest system.
RESULTS:
The neurobehavioral ability and balance function declined 1 h later after dosing estazolam. The neurobehavioral ability index and balance function declined to the lowest level 3 h later after dosing estazolam. The neurobehavioral ability recovered partly 6 h later after dosing estazolam, and neurobehavioral ability recovered completely 10 h later.
CONCLUSION
Driving ability was impaired when estazolam concentration in blood is 20 ng/mL, and the neurobehavioral ability declined when estazolam concentration is 40 ng/mL in blood. The influence to human in absorption process is greater than the metabolic process with the same estazolam concentration.
Accidents, Traffic/prevention & control*
;
Administration, Oral
;
Adult
;
Anticonvulsants/pharmacokinetics*
;
Attention/drug effects*
;
Behavior/drug effects*
;
Estazolam/pharmacokinetics*
;
Female
;
Humans
;
Male
;
Neuropsychological Tests
;
Psychomotor Performance/drug effects*
;
Reaction Time
4.Fluoxetine is Neuroprotective in Early Brain Injury via its Anti-inflammatory and Anti-apoptotic Effects in a Rat Experimental Subarachnoid Hemorrhage Model.
Hui-Min HU ; Bin LI ; Xiao-Dong WANG ; Yun-Shan GUO ; Hua HUI ; Hai-Ping ZHANG ; Biao WANG ; Da-Geng HUANG ; Ding-Jun HAO
Neuroscience Bulletin 2018;34(6):951-962
Fluoxetine, an anti-depressant drug, has recently been shown to provide neuroprotection in central nervous system injury, but its roles in subarachnoid hemorrhage (SAH) remain unclear. In this study, we aimed to evaluate whether fluoxetine attenuates early brain injury (EBI) after SAH. We demonstrated that intraperitoneal injection of fluoxetine (10 mg/kg per day) significantly attenuated brain edema and blood-brain barrier (BBB) disruption, microglial activation, and neuronal apoptosis in EBI after experimental SAH, as evidenced by the reduction of brain water content and Evans blue dye extravasation, prevention of disruption of the tight junction proteins zonula occludens-1, claudin-5, and occludin, a decrease of cells staining positive for Iba-1, ED-1, and TUNEL and a decline in IL-1β, IL-6, TNF-α, MDA, 3-nitrotyrosine, and 8-OHDG levels. Moreover, fluoxetine significantly improved the neurological deficits of EBI and long-term sensorimotor behavioral deficits following SAH in a rat model. These results indicated that fluoxetine has a neuroprotective effect after experimental SAH.
Animals
;
Apoptosis
;
drug effects
;
Blood-Brain Barrier
;
drug effects
;
Brain Edema
;
drug therapy
;
etiology
;
Cytokines
;
genetics
;
metabolism
;
Disease Models, Animal
;
Fluoxetine
;
pharmacology
;
therapeutic use
;
In Situ Nick-End Labeling
;
Male
;
Neuroprotective Agents
;
pharmacology
;
therapeutic use
;
Pain Measurement
;
Psychomotor Performance
;
drug effects
;
RNA, Messenger
;
metabolism
;
Rats
;
Rats, Sprague-Dawley
;
Subarachnoid Hemorrhage
;
complications
;
drug therapy
;
pathology
;
Time Factors
;
Vasospasm, Intracranial
;
drug therapy
;
etiology