OBJECTIVETo study the total content change of psoralea and isopsoralea in Psoralea corylifolia under different temperature, and predict its term of validity by initial average rate stability test in order to provide the basis for assessing its quality.

METHODThe reaction rate constant K was replaced by initial velocity V. According to Arrhenius rule, storage period was obtained at room temperature.

RESULTThe stable life of P. corylifolia has been determined as 0.53 years.

CONCLUSIONThe high temperature is not conducive to the stability of the P. corylifolia, which need to store in a dark and cool place.

Drug Stability ; Psoralea ; chemistry ; Temperature

The present study established a determination method of Psoraleae Fructus by quantitative analysis of multi-components by the single marker(QAMS) and further improved the thin-layer chromatography(TLC) method. The QAMS method was established by UPLC with psoralen as the internal marker, and the content of psoralenoside, isopsoralenoside, psoralen, and isopsoralen was simultaneously determined. As revealed by the comparison with results of the external standard method, the QAMS method was accurate and feasible. According to the current quality standards of Psoraleae Fructus, the TLC method was further optimized and improved, and bakuchiol was added for identification based on the original TLC method with psoralen and isopsoralen as indicators. This study provides a reference for improving the quality control method of Psoraleae Fructus.
Chromatography, High Pressure Liquid/methods* ; Drugs, Chinese Herbal/analysis* ; Ficusin ; Fruit/chemistry* ; Furocoumarins/analysis* ; Psoralea

With the rise of incidence, fatality rate, and number of young cases, diabetes mellitus has been one of the seven major diseases threatening human health. Although many antidiabetic drugs(oral or for injection) are available, the majority have serious side effects during the long-term use. Thus, it is of particularly vital to develop new drugs with low risk and definite effect. Psoraleae Fructus, a traditional medicinal widely used in the folk, has hypoglycemic, anti-osteoporosis, antitumor, estrogen-like, and anti-inflammatory effects. Thus, it has great clinical application potential. Chinese medicine and the active ingredients, characterized by multiple targets, multiple pathways, and multiple effects in the treatment of diabetes mellitus, have distinct advantages in clinical application. However, the safety of Chinese medicine remains to be a challenge, and one of keys is to clarifying the mechanism of a single Chinese medicinal and its active ingredients. With the method of literature research, this study summarized and analyzed the hypoglycemic mechanisms of Psoraleae Fructus and its main active ingredients over the last decade: regulating glucose metabolism, improving insulin resistance, and directly acting on pancreatic β-cells. The result is expected to serve as a reference for further research on the effects of Psoraleae Fructus and its main chemical constituents in lowering blood glucose and preventing diabetes mellitus and the clinical application.
Drugs, Chinese Herbal/pharmacology* ; Fruit/chemistry* ; Humans ; Hypoglycemic Agents/pharmacology* ; Osteoporosis/drug therapy* ; Psoralea/chemistry*

OBJECTIVETo identify the compounds in fruits of Psoralea corylifolia by high performance liquid chromatography-mass spectrometry.

METHODThe chromatographic separation was carried out at 30 degrees C on a Diamonsil C18 column (4.6 mm x 250 mm, 5 microm) eluted in the gradient program. The mobile phase consisted of acetonitrile (A) and water containing 0.05% formic acid (B). The detection wavelength was at 246 nm and the on-line UV spectra was recorded in the range of 190-400 nm. The mass spectra were obtained by Finnigan LCQ Deca XP(plus) ion trap mass spectrometer in the positive ion mode with ion spray voltage 4.5 kV, sheath gas (N2), 60 arbitrary units, auxiliary gas (N2), 20 arbitrary units; capillary temperature, 350 degrees C, capillary voltage 19 V, tube lens offset voltage 25 V, mass range recorded, m/z 90-800.

RESULTTwo coumarin glycosides, three coumarins, eight flavonoids and one meroterpene were identified by HPLC-MS method.

CONCLUSIONThe method is simple and rapid for the identification and quality control for the fruits of P. corylifolia.

Chromatography, High Pressure Liquid ; methods ; Fruit ; chemistry ; Mass Spectrometry ; methods ; Plant Extracts ; chemistry ; Psoralea ; chemistry

UNLABELLEDOBJECITVE: To established an efficient method for simultaneous quantification of 7 compounds belonging to 4 chemical types in Psoralea corylifolia processed by different methods, and to elucidate variations of 4 kinds of compounds in different processed P. corylifolia.

METHODThe chromatographic separation was performed on an ACUITY C18 column using acetonitrile and 0.1% formic acid aqueous solution in the gradient elution at 0.4 mL x min(-1). Detection wavelength was set at 246 nm. Column temperature was fixed at 50 degrees C.

RESULTThe 4 kinds of compounds including psoralenoside, isopsoralenoside (benzofuran glycosides), psoralen, isopsoralen, psoralidin (coumarins), bavachin (flavonoids), and bakuchiol (meroterpenes) were separated in 25 min. The correlation coefficients of those compounds were over 0.9993 in the tested range. The intra- and inter-day precisions were below 1.5%. The average recoveries ranged from 99.2% to 106%.

CONCLUSIONThis method is simple, rapid and accurate, which can be used for the determination of the 4 kinds of compounds in different processed P. corylifolia. In P. corylifolia processed by different methods, the contents of benzofuran glycosides, flavonoids, and meroterpenes in P. corylifolia processed by Leigong's method decrease obviously, while the contents of psoralen and isopsoralen increase significantly.

Chromatography, High Pressure Liquid ; Drug Stability ; Plants, Medicinal ; chemistry ; Psoralea ; chemistry ; Reproducibility of Results ; Technology, Pharmaceutical

OBJECTIVETo establish a convenient and rapid method of microwave-assisted extraction (MAE) for the extraction of psoralen and isopsoralen in Psoralea corylifolia.

METHODThe effects of various parameters on the MAE were investigated with several single-factor tests, and compared with the traditional extraction.

RESULTThe optimal conditions of MAE were as follows: the extractine time was 3 min, and the ratio of solvent to material was 400 mL x g(-1) by using methanol as the solvent. Compared with soxhlet extraction, heat reflux extraction, and ultrasound-assisted extraction, MAE needed only 3 min to give the highest yield of psoralen and isopsoralen, while the other extraction methods needed several hours and gave lower yield.

CONCLUSIONThe proposed method has greatly shortened extraction time and improved extraction yield, so it provides a neotype, simple and rapid measure for the separation of psoralen and isopsoralen from P. corylifolia.

Chromatography, High Pressure Liquid ; Ficusin ; chemistry ; Furocoumarins ; chemistry ; Microwaves ; Psoralea ; chemistry ; Reproducibility of Results

OBJECTIVETo determine two flavonoid compounds in Psoralea corylifolia L. (PC) simultaneously with HPLC method.

METHODBavachin and corylin isolated from PC and purified in our laboratory were used as the reference compounds. The HPLC separation was carried out on an Techsphere ODS column using mobile phase consisting of a mixture of methanol and 20 mmol.L-1 ammonium acetate buffer pH 4.0 (67:33), and the UV detection wavelength was 240 nm.

RESULTSimultaneous determination of bavachin and corylin was achieved. The linear range was 1.25-20 micrograms.mL-1 for both bavachin and corylin. The average recovery of bavachin and corylin was 94.9% and 96.2%, and RSD was 3.1% and 3.6% respectively.

CONCLUSIONThis is the first report on simultaneous determination of bavachin and corylin in PC with satisfactory accuracy and reproducibility.

Chromatography, High Pressure Liquid ; Flavonoids ; analysis ; Fruit ; chemistry ; Plants, Medicinal ; chemistry ; Psoralea ; chemistry

The traditional Chinese medicine standard decoction is prepared on the basis of the theory of traditional Chinese medicine and clinical application. With reference to the modern extraction method,the single decoction of traditional Chinese medicine is prepared by the standardized process,and the establishment of its quality standards is conducive to standardizing clinical medication. This research is to set an evaluation standard for the quality of salt-processed Psoraleae Fructus standard decoction. Twelve batches of salt-processed Psoraleae Fructus standard decoctions were prepared. The contents of psoralen and isopsoralen were determined,the transfer and extract rates were calculated,and the pH value was measured; HPLC fingerprint method was established for analysis. The results of the 12 batches of samples revealed that the transfer rates of psoralen and isopsoralen were 17. 10%-26. 40%,14. 70%-22. 70%,respectively; the extract rate was between 14. 7%-27. 0%,and the pH value was between 5. 4-6. 9. Moreover,7 common chromatographic peaks were determined based on fingerprint by using similarity evaluation system for chromatographic fingerprint of traditional Chinese medicine( 2012 A).The similarities of the 12 batches of samples were analyzed and compared,and the results showed that the similarities were all higher than0. 9. In this study,the preparation method for salt-processed Psoraleae Fructus decoction was standard,with high similarities in fingerprint. This study build a convenient and reliable method of comprehensive quality evaluation,with a high precision,stability and repeatability,which can provide a reference for the quality control of salt-processed Psoraleae Fructus dispensing granules.
Chromatography, High Pressure Liquid ; Drugs, Chinese Herbal ; analysis ; Fruit ; chemistry ; Medicine, Chinese Traditional ; Phytochemicals ; analysis ; Psoralea ; chemistry ; Quality Control

The potential hepatotoxicity of herbal remedies is usually ignored in daily life. One such compound, Boh-Gol-Zhee (in Chinese, Bu Ku Zi), appeared to be the cause for acute cholestatic hepatic injury in the following case. Psoralea corylifolia is known to contain a sufficient amount of phyto-estrogen. Some alternative medicine therapists claim that Psoralea corylifolia is effective for the treatment of osteoporosis. So far, at least 35 chemical components in the fruits of Psoralea corylifolia have been identified. High concentrations of phyto-estrogen, such as genistein, have been found in the leaves of Psoralea corylifolia. Phyto-estrogen has structural similarity to estrogen and exhibits beneficial effects on osteoporosis under estrogen-deficient conditions. The authors observed a case of acute cholestatic hepatitis in a post-menopausal woman caused by seeds of Psoralea corylifolia in amounts over 10 times the usual dose. Other possible causes of liver injury were excluded by laboratory tests and imaging studies. After discontinuation of this remedy, elevated liver enzyme and total bilirubin rapidly returned to normal. Liver biopsy showed zone 3 necrosis, degenerating cells, cholestasis and infiltrations with inflammatory cells. This case stresses the need to warn the potential hepatotoxicity of Psoralea corylifolia seeds, especially in a large dose.
Asian Continental Ancestry Group ; Bilirubin ; Biopsy ; Cholestasis ; Complementary Therapies ; Estrogens ; Female ; Fruit ; Genistein ; Hepatitis* ; Humans ; Liver ; Necrosis ; Osteoporosis ; Phytoestrogens ; Psoralea

To study the chemical constituents of the fruits of Psoralea corylifolia L., the constituents were isolated and purified by chromatography. Two new compounds were isolated from the ethyl acetate-soluble fraction of the ethanol extract. On the basis of chemical and spectroscopic data 1D and 2D NMR, they were identified as 2-(3, 4-dihydroxyphenyl)-8, 8-dimethyl-8H-pyrano[2, 3-h]chromen-4-one (1) and 5-(2-hydroxyl-1-methyl)-ethyl-4, 5-dihydrofurano[2, 3-h](4'-hydroxyl-3', 5'-dimethoxy)-isoflavone (2), and named as coryfolia D and bavarigenin separately.
Chromones ; chemistry ; isolation & purification ; Fruit ; chemistry ; Isoflavones ; chemistry ; isolation & purification ; Molecular Conformation ; Molecular Structure ; Plants, Medicinal ; chemistry ; Psoralea ; chemistry