No abstract available.
Herpesvirus 1, Suid* ; Pseudorabies*

No abstract available.
Baculoviridae* ; Herpesvirus 1, Suid* ; Pseudorabies*

Aujeszky's disease caused by Aujeszky's disease virus (ADV) is one of the most important diseases in the pig industry. In this study, we conducted a seroepidemiological survey of ADV in wild boars and raccoon dogs in South Korea. In total, 217 wild boar sera collected between March and August 2013, and 96 raccoon dogs between 2011 and 2012 were screened for the presence of antibodies against ADV. The sero-positive rates in wild boars and raccoon dogs tested for ADV were found to be 3.55% (8/225) and 0% (0/96), respectively. The presence of virus neutralization antibody titer against ADV means that small number of wild boars was infected with ADV and AD may be circulated continuously in Korean wild boar populations, and that wild boars may act as a potential reservoir of ADV. Therefore, to achieve the declaration of AD free, effective preventive measures to block transmission of AD should be taken to the wild boars.
Antibodies ; Herpesvirus 1, Suid* ; Korea ; Pseudorabies ; Raccoon Dogs* ; Sus scrofa*

No abstract available.
Animals ; Axis, Cervical Vertebra* ; Herpesvirus 1, Suid* ; Pseudorabies* ; Rats* ; Uterus*

With the application of gE gene deleted pseudarabies virus (PRV) vaccine worldwide, a corresponding differential diagnosis based on gE glycoprotein is needed in the project of PRV eradication. In this study, PRV gE gene without signal and transmembrane region was amplified by PCR and cloned into pGEX-6P-1, generated pGEX-gE. After transformation of BL21 with pGEX-gE, an expressed fusion protein(about 63kD) was identified by SDS-PAGE. The recombinant proteins are produced as inclusion bodies. By changing the inductive conditions, the formation of inclusion bodies was inhibited and tended to increase the percentage of soluble recombinant protein. The antigenic reactivity of the recombinant protein was confirmed by Western blotting with polyclonal antibodies against PRV. Using purified recombinant tgE as antigen, an ELISA was developed to detect sera of PRV infected pigs and sera of pigs immunized with gE-deleted PRV vaccine. The total of 400 serum samples collected from field were comparatively tested with the tgE-ELISA and a commercial competitive ELISA based on monoclonal antibody against gE, the results indicated that the coincidental rate between the two tests is about 94%.
Animals ; Diagnosis, Differential ; Enzyme-Linked Immunosorbent Assay ; Herpesvirus 1, Suid ; immunology ; Pseudorabies ; diagnosis ; Pseudorabies Vaccines ; immunology ; Recombinant Proteins ; biosynthesis ; immunology ; Swine ; Vaccination ; Viral Envelope Proteins ; genetics ; immunology

We designed two pairs of primers and their corresponding TaqMan probes according to gH, gE gene of PRV. By optimizing the probe's concentration, Mg2+ concentration, primers concentration and sample DNA extraction, real-time fluorescent quantitative PCR (FQ-PCR) which can quickly identity field virus and vaccine virus of PRV was established. According to our results, the dynamic range of the FQ-PCR assay is between 10 x 10(1) copies/microL and 10 x l0(8) copies/microL, and the detection limit of FQ-PCR is 1.0 x 10(1) copies/microL, which is 100 fold higher than that of conventional PCR. We detected 60 doubtful tissue samples using the FQ-PCR assay, serum neutralization and conventional PCR. In conclusion, the FQ-PCR method is rapid, sensitive, specific and accurate, and can be used to detect field strains of PRV rapidly. The closed-tube format of the assay minimized the risk of contamination of subsequent reaction and the assay can be performed in 2 h or less. Development of real-time quantitative PCR provides the basis for the early and rapid detection and analyzing quantitatively the infectious degree of PRV.
Animals ; Fluorescent Dyes ; Herpesvirus 1, Suid ; genetics ; isolation & purification ; Polymerase Chain Reaction ; methods ; Pseudorabies ; diagnosis ; prevention & control ; virology ; Pseudorabies Vaccines ; immunology ; isolation & purification ; Swine

Outbreaks of pseudorabies (PR) have occurred in southern China since late 2011, resulting in significant economic impacts on the swine industry. To identify the cause of PR outbreaks, especially among vaccinated pigs, 11 pseudorabies virus (PRV) field strains were isolated from Guangdong province during 2013–2014. Their major viral genes (gE, TK, gI, PK, gD, 11K, and 28K) were analyzed in this study. Insertions or deletions were observed in gD, gE, gI and PK genes compared with other PRV isolates from all over the world. Furthermore, sequence alignment showed that insertions in gD and gE were unique molecular characteristics of the new prevalent PRV strains in China. Phylogenetic analysis showed that our isolates were clustered in an independent branch together with other strains isolated from China in recent years, and that they showed a closer genetic relationship with earlier isolates from Asia. Our results suggest that these isolates are novel PRV variants with unique molecular signatures.
Asia ; China* ; Disease Outbreaks ; Genes, Viral ; Herpesvirus 1, Suid* ; Pseudorabies* ; Sequence Alignment ; Swine

A cross-sectional serological study was conducted in Shandong province of China to determine the seroprevalence and risk factors associated with seropositivity due to pseudorabies virus (PRV) infection in small- and medium-sized farrow-to-finish herds following outbreaks of variant PRV strains. A total of 6,035 blood samples from 224 randomly selected herds were screened. The results showed that 25.0% of the herds and 56.7% of the serum samples were seropositive for field strains of PRV. Herds consisting of 50–100 breeding sows had higher herd seroprevalence and serum sample seroprevalence than larger herds. Both the highest herd seroprevalence and highest serum sample seroprevalence were observed in western Shandong, followed northern Shandong. Based on univariate analysis, the following risk factors were utilized in subsequent multivariable logistic regression analysis: region, herd size, weight of purchased gilts, and all-in/all-out practice. Upon multivariate analysis, region, herd size, weight of purchased gilts and all-in/all-out practice were significantly associated with PRV herd seropositivity. These findings indicate that we are facing a serious situation in the prevention and control of pseudorabies. The results could help predict the next outbreak and set out control measures.
Breeding ; China* ; Disease Outbreaks ; Herpesvirus 1, Suid ; Logistic Models ; Multivariate Analysis ; Pseudorabies* ; Risk Factors* ; Seroepidemiologic Studies*

We investigated the location of neurons related to lateral rectus muscle in the cerebellum of rats. Horseradish peroxidase (HRP) and Pseudorabies virus (PRV) were injected into right lateral rectus muscle of rats. After 84~90 hours, cardiac perfusion was performed with 4% paraformaldehyde in HRP injection group and 4% paraformaldehyde-lysine-periodate in PRV injection group. Tetramethylbenzidine (TMB) neurohistochemical stain in HRP injection group and immuno-histochemical stain in PRV injection group were performed after frozen section. HRP-reactive neuronal cells were observed in ipsilateral abducens nucleus. PRV-immunoreactive neuronal cells were observed ipsilaterally in abducens nucleus, flocculus, paraflocculus, ansiform lobule, declive, dentate nucleus, infracerebellar nucleus and y nucleus and also bilaterally in nodulus, lobulus centralis, lingula, uvula vermis and fastigial nucleus. These findings show the existence of neuronal projection from the abducens nucleus to the cerebellum. This projection could be part of nerve circuits through which the cerebellum modulates visuomotor activities.
Animals ; Cerebellar Nuclei ; Cerebellum* ; Frozen Sections ; Herpesvirus 1, Suid* ; Horseradish Peroxidase ; Neurons* ; Perfusion ; Pseudorabies* ; Rats* ; Uvula

With its abilities of trans-synaptic tracing and self-replication and wide host range, pseudorabies virus (PRV) has been applied in the field of neuroanatomy since the 1970s. Four decades of PRV application have made many advances in researches on neuronal tracing with PRV. Mechanism studies focused on investigating infection of primary neurons and tracing direction in secondary neurons, while application studies focused on development of new pathological strains and innovation of tracing techniques. To date, the mechanism and application of viral tracing are not completely figured out yet. Integration of molecular biology technology will improve the efficiency in related researches.
Animals ; Cell Tracking ; Herpesvirus 1, Suid ; genetics ; physiology ; Humans ; Neurons ; virology ; Pseudorabies ; virology