1.Synthesis and characterization of alginate oligosaccharides produced by Pseudomonas mendocina NK-01.
Wenbin GUO ; Shufang WANG ; Mingfeng CAO ; Weitao GENG ; Cunjiang SONG
Chinese Journal of Biotechnology 2009;25(9):1366-1370
In this study, we found that when Pseudomonas mendocina NK-01 accumulated intracellular carbon reserve, medium chain length poly (3-hydroxyalkanoates), it also synthesized extracellular saccharides, alginate oligosaccharides. The high carbon nitrogen ratio of culture medium facilitated alginate oligosaccharides production. We analyzed the structure of alginate oligosaccharide by Ultraviolet-Visible Spectrophotometry, Fourier Transform Infrared Spectroscopy, 1H and 13C of Nuclear Magnetic Resonance, and found that it was compounded in line from beta-D-mannuronic acids and alpha-L-gluronic acids via beta-(1-->4)/ alpha-(1-->4) bonds, which acetylated partly on the 2- and/or 3-hydroxy. In addition, we determined the weight-average molecular weight of alginate oligosaccharides by gel permeation chromatography to be 2054.
Alginates
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chemistry
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Glucuronic Acid
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biosynthesis
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chemistry
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genetics
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Hexuronic Acids
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chemistry
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Molecular Weight
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Oligosaccharides
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biosynthesis
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chemistry
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genetics
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Pseudomonas mendocina
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metabolism