Positive bacterial culture was obtained in 33 out of 70 cultures of the tip of used Foley catheter, performed during the period of 20 months from March l st, 1977 to October 31st, 1978 and the following results were obtained. l. Almost twice much positive bacterial culture was obtained from the culture of used catheter tip (45. 7%) compared with the urine culture taken from the same patient at the same time (24. 3%). 2. Positive culture rate of the catheter tip in the group who was catheterized at operating room (32.7%) was much lower than in the group catheterized at ward (76.2%). Very few positive culture was obtained from the catheter tip used in a patient who had normal urine findings previous to catheterization if this indwelling catheter removed within 72 hours. 4. The most common organism on the culture of the catheter tip was proteus sp. (9 cases), and less commonly, pseudomonas (3 cases), enterococci (3 cases), yeast (3 cases), E. coli (2 cases), klebsiella (2 cases), alcaligenes (2 cases), enterobact.(2 cases) and citrobact.(2 cases) were found.
Alcaligenes ; Catheterization ; Catheters* ; Catheters, Indwelling ; Humans ; Klebsiella ; Operating Rooms ; Proteus ; Pseudomonas ; Yeasts

In this research a strain of isolated Pseudomonas alcaligenes which causes degradation of dexamethasone was acclimated further and its proteins of every position in the bacterium were separated by the osmotic shock method. The separated intracellular proteins which had the highest enzyme activity were extracted by the salting out with ammonium sulfate and were purified with the cation exchange chromatography and gel chromatography. The purified proteins which was active to cause degradation of dexamethasone had been detected were cut with enzyme and were analyzed with mass spectrometry. The results showed that the degradation rate to dexamethasone by acclimated Pseudomonas alcaligenes were increased from 23.63% to 52.84%. The degrading enzymes were located mainly in the intracellular of the bacteria and its molecular weight was about 41 kD. The specific activity of the purified degrading enzymes were achieved to 1.02 U x mg(-1). Its 5-peptide amino acid sequences were consistent with some sequences of the isovaleryl-CoA dehydrogenase. The protein enzyme may be a new kind degrading enzyme of steroidal compounds. Our experimental results provided new strategies for cleanup of dexamethasone in water environment with microbial bioremediation technique.
Amino Acid Sequence ; Bacterial Proteins ; chemistry ; Chromatography ; Dexamethasone ; chemistry ; Molecular Weight ; Pseudomonas alcaligenes ; enzymology

A bacteriological study was made on 21 cases of the chronic prostatitis suspected by subjective symptoms, microscopic findings of urine, rectal findings of prostate and microscopic findings of prostatic secretion during the period from June to October 1980, and clinical observation was also performed. The results were summarized as follows; 1. The distribution of age showed the highest incidence in 20 to 39 years (16 cases: 76.2%). 2. 13 cases (61.9%) of patients had previously experienced acute or chronic urethritis. 3. Major subjective symptoms consisted of pollakisuria (12 cases: 57.1%), perineal discomfort and urethral discomfort in order of frequency. 4. On rectal palpation of prostate, 18 cases (85.7%) were abnormal in consistency. Most of the prostate were normal in size, but 9 cases enlarged and 1 case was small. 5. Majority of the cases (18 cases: 85.7%) were normal in microscopic examination of urine. 6. In microscopic findings of prostatic secretion, 17 cases (81.0%) showed W.B.C. more than 10/H.P.F. 7. In 21 cases in which urine and prostatic secretion culture were done, microorganisms were cultured in 9 cases (42.9%) and no growth was noted in 12 cases (57.1%). There were 3 cases of mixed infection. The isolated microorganisms revealed 6 cases of Staphylococcus aureus, 3 cases of E. coli, 1 case of Staphylococcus epidermidis, 1 case of Pseudomonas fluoresces and 1 case of Alcaligenes. 8. The mean colony counts were as follows. VB1: 881+/-583 colonies/ml VB2: 123+/-112 colonies/ml EPS: 57,778+/-28,299 colonies/ml VB3: 5,389+/-4,745 colonies/ml There was more predominant growth in EPS and VB3 than VB1 and VB2. 9. Microscopic findings of biopsied prostatic tissue showed infiltration of chronic inflammatory cells in all 4 cases. The results of bacterial culture of prostatic tissue were negative in 2 cases, Staphylococcus aureus in 1 case and E. coli in 1 case. The isolated microorganisms were identical to prostatic secretion cultures. 10. The pH of prostatic secretions were 14 cases (66.7%) in 7.7 to 8.0 and 4 cases (19.0%) in over 8.0. There was no significant difference in pH of prostatic secretions between prostatic secretion culture positive group and culture negative group.
Alcaligenes ; Bacteriology ; Coinfection ; Digital Rectal Examination ; Humans ; Hydrogen-Ion Concentration ; Incidence ; Prostate ; Prostatitis* ; Pseudomonas ; Staphylococcus aureus ; Staphylococcus epidermidis ; Urethritis

Two hundred fifty bacterias isolated in significant numbers from 217 specimens of urine and 79 organisms from 79 acute urethral discharge between Jan. 1973 and Dec. 1973. were evaluated and the following results were obtained. 1. Of a total of 250 bacterial strains isolated by cultures of the urine. 87.2% was gram-negative bacteria and 12.8% was gram-positive. Isolated organisms include E. coli (33.6%), Klebsiella(14.8%), Colifom bacilli (11.2%), .Alcaligenes (10%), Streptococcus (7.6%), Pseudomonas (6%) and Proteus (5.2%) in order of frequency. 2. There was a striking difference between the bacterial flora in the urine of patients with acute urinary tract infection and that in the urine of patients with chronic urinary tract infection. Acute urinary tract infection was mainly caused by a strain of E. coli (53.1%). In chronic urinary tract infection, the relative incidence of infection caused by more antimicrobial-resistant gram-negative barcilli such as Klebsiella, Pseudomonas, Proteus, and Alcaligenes and that of infection caused by E. coli was 21.25. Sum of Klebsiella, Pseudomonas, Proteus, and Alcaligenes in acute urinary tract infection was 14.1% and in chronic urinary tract infection was 45. 5%. 3. Above the 85% of Klebsiella, Pseudomonas, and Alcaligenes was resistant to six kinds of antibiotics, which were Penicillin G., Streptomycin. Chloramphtnicol, Tetracycline, Kanamycin and AmpiciIlin, 67.2% of 8. coli and 54.8% of Proteus were resistant to that.
Alcaligenes ; Anti-Bacterial Agents ; Bacteria ; Gram-Negative Bacteria ; Humans ; Incidence ; Kanamycin ; Klebsiella ; Penicillin G ; Proteus ; Pseudomonas ; Streptococcus ; Streptomycin ; Strikes, Employee ; Tetracycline ; Urinary Tract Infections* ; Urinary Tract*

Two hundred fifty bacterias isolated in significant numbers from 217 specimens of urine and 79 organisms from 79 acute urethral discharge between Jan. 1973 and Dec. 1973. were evaluated and the following results were obtained. 1. Of a total of 250 bacterial strains isolated by cultures of the urine. 87.2% was gram-negative bacteria and 12.8% was gram-positive. Isolated organisms include E. coli (33.6%), Klebsiella(14.8%), Colifom bacilli (11.2%), .Alcaligenes (10%), Streptococcus (7.6%), Pseudomonas (6%) and Proteus (5.2%) in order of frequency. 2. There was a striking difference between the bacterial flora in the urine of patients with acute urinary tract infection and that in the urine of patients with chronic urinary tract infection. Acute urinary tract infection was mainly caused by a strain of E. coli (53.1%). In chronic urinary tract infection, the relative incidence of infection caused by more antimicrobial-resistant gram-negative barcilli such as Klebsiella, Pseudomonas, Proteus, and Alcaligenes and that of infection caused by E. coli was 21.25. Sum of Klebsiella, Pseudomonas, Proteus, and Alcaligenes in acute urinary tract infection was 14.1% and in chronic urinary tract infection was 45. 5%. 3. Above the 85% of Klebsiella, Pseudomonas, and Alcaligenes was resistant to six kinds of antibiotics, which were Penicillin G., Streptomycin. Chloramphtnicol, Tetracycline, Kanamycin and AmpiciIlin, 67.2% of 8. coli and 54.8% of Proteus were resistant to that.
Alcaligenes ; Anti-Bacterial Agents ; Bacteria ; Gram-Negative Bacteria ; Humans ; Incidence ; Kanamycin ; Klebsiella ; Penicillin G ; Proteus ; Pseudomonas ; Streptococcus ; Streptomycin ; Strikes, Employee ; Tetracycline ; Urinary Tract Infections* ; Urinary Tract*