Brain Diseases ; complications ; microbiology ; mortality ; Fatal Outcome ; Female ; Humans ; Middle Aged ; Otitis Externa ; complications ; microbiology ; mortality ; Pseudomonas Infections ; complications ; mortality

Ecthyma gangrenosum is a characteristic skin lesion of systemic infection due to Pseudomonas aeruginosa. It has a high incidence in patients with chronic disease and impaired defense mechanisms. Early diagnosis and appropriate systemic antibiotic therapy is crucial since its mortality rate is very high. We report a case of ecthyma gangrenosum in aplastic anemia.
Adult ; Anemia, Aplastic/*complications/pathology ; Case Report ; Female ; Human ; Opportunistic Infections/microbiology/pathology ; Pseudomonas Infections/*complications/microbiology/pathology ; Skin Diseases, Bacterial/drug therapy/*etiology/pathology

Although Pseudomonas aeruginosa is not generally considered as a cause of antibiotic-associated diarrhea, several cases of diarrhea caused by P. aeruginosa have been reported. We experienced seven cases of nosocomial diarrhea presumably caused by P. aeruginosa, which was the predominant organism isolated from stool cultures. Clostridium difficile toxin was also positive in one patient. No other potential or recognized enteropathogens were identified from stools. All patients had underlying diseases and had been receiving antibiotics before the diarrheal onset. All of the seven P. aeruginosa isolates were resistant to previously given antibiotics. Diarrhea stopped three days after withdrawal of probable offending antibiotics without specific treatment in two patients. The other five patients having continuous diarrhea despite withdrawal of probable offending antibiotics, were successfully treated with antipseudomonal agents. The median duration of diarrhea after the initiation of treatment was 6.3 days. These data suggest that P. aeruginosa can be a potential cause of antibiotic-associated diarrhea. Further investigations are warranted to evaluate the possible etiologic role of P. aeruginosa in antibiotic-associated diarrhea.
Adult ; Aged ; Antibiotics/*adverse effects ; Colitis/complications/drug therapy ; Cross Infection/complications ; Diarrhea/*chemically induced/*microbiology ; Feces/microbiology ; Female ; Human ; Male ; Middle Age ; Pseudomonas Infections/*complications ; *Pseudomonas aeruginosa ; Retrospective Studies

Anthracosis ; complications ; microbiology ; Anti-Bacterial Agents ; pharmacology ; Drug Resistance, Bacterial ; Humans ; Imipenem ; pharmacology ; Microbial Sensitivity Tests ; Pseudomonas Infections ; complications ; drug therapy ; microbiology ; Pseudomonas aeruginosa ; drug effects ; Pulmonary Heart Disease ; complications ; microbiology

OBJECTIVE: To analyze the factors and biological characteristics of infection after cochlear implantation so as to control the risk factors and improve the treatment of postoperative infection. METHOD: A retrospective study was conducted to analyze the clinical data of 316 patients receiving cochlear implantation from July 2001 to October 2011. RESULT: Postoperative infection was found in five of the 316 cases and one transferred case. The six cases recovered after clinical therapy without explantation. One case underwent explantation due to recurrent meningitis after implantation of 8 years later. CONCLUSION The pathogens of infection after cochlear implantation are staphylococcus aureus, pseudomonas aeruginosa, etc. The key infectious factor is the formation of bacterial biofilm, which can be removal by chemical agents to control the postoperative infection, especially the flap infection. It is not necessary to remove the artificial cochlea when the postoperative infection occurs. Positive perioperative interventions and postoperative infection control can improve the outcome of cochlear implantation.
Adolescent ; Aged ; Biofilms ; growth & development ; Cochlea ; Cochlear Implantation ; adverse effects ; Humans ; Postoperative Complications ; microbiology ; Pseudomonas Infections ; etiology ; Pseudomonas aeruginosa ; physiology ; Retrospective Studies ; Risk Factors ; Staphylococcal Infections ; etiology ; Staphylococcus aureus ; physiology

Adult ; Aged ; Aged, 80 and over ; Anthracosis ; complications ; microbiology ; Anti-Bacterial Agents ; pharmacology ; Drug Resistance, Bacterial ; Humans ; Lung Neoplasms ; complications ; microbiology ; Male ; Middle Aged ; Pseudomonas Infections ; microbiology ; Pseudomonas aeruginosa ; drug effects

OBJECTIVETo investigate the efficacy of allogeneic peripheral blood stem cell transplantation (PBSCT) in the treatment of severe aplastic anemia (SAA) and severe infection.

METHODSA patient with SAA and pseudomonas aeruginosa septicemia was treated with PBSCT from an HLA-identical sibling with cyclophosphamide (CY) and total body irradiation (TBI) for conditioning. The patient was infused with 20.3 x 10(8)/kg mononuclear cells including 61.0 x 10(6)/kg CD34(+) cells following the conditioning regimen.

RESULTSTwelve days after PBSCT, the absolute neutrophil count (ANC) of 1.0 x 10(9)/L was achieved, with platelet count > 50 x 10(9)/L at twenty days. The donor origin of engraftment was confirmed by polymerase chain reaction (PCR) analysis of short tandem repeats at the end of the first, sixth and twelfth month. The patient's body temperature dropped to normal level when her ANC reached 0.5 x 10(9)/L on day 10, and the bacterial culture of blood sample became negative subsequently. Symptoms and signs of acute or chronic graft versus host disease (GVHD) were not observed in 30 months after PBSCT.

CONCLUSIONSHematopoiesis was reconstituted shortly after PBSCT. The combination of CY and TBI and the infusion of sufficient peripheral blood stem cells may contribute to the successful engraftment. PBSCT may be considered as the first choice when hematopoietic stem cell transplantation is needed for SAA patients complicated with severe infection.

Adult ; Anemia, Aplastic ; immunology ; therapy ; Bacteremia ; complications ; Female ; Humans ; Peripheral Blood Stem Cell Transplantation ; methods ; Prognosis ; Pseudomonas Infections ; complications ; Transplantation, Homologous ; immunology

OBJECTIVETo examine the expression profile of lipid metabolism-related genes in liver of scalded rats with wound sepsis, and to analyze its significance.

METHODSSixty male Wistar rats with 30% TBSA full-thickness scald wound on the back were enrolled in the study and randomly divided into wound sepsis group (n = 30) and control group (n = 30, with scald). Pseudomonas aeruginosa was inoculated to the wounds in sepsis group. Corresponding indices were determined to verify the diagnosis of wound sepsis. The rats were sacrificed and fresh liver tissues were obtained at 96 post-scald hours (PSH). Total RNA of liver was isolated with Trizol and the different expression of lipid metabolism related genes in response to burn wound sepsis was assessed by DNA microarray.

RESULTSBy comparing expression profile of the two groups, totally 47 genes were observed to be differentially expressed in rat hepatic tissues, among them 9 genes were related to lipid metabolism. Among them, those which were upregulated were genes in relation with transportation and activation of fatty acid, and those downregulated were genes related in providing energy for fatty acid oxidation in mitochondria.

CONCLUSIONThe occurrence of wound sepsis in scalded rats can induce changes in the expression of hepatic lipid metabolism related genes in hepatic tissues, and aggravate

Animals ; Burns ; complications ; metabolism ; Gene Expression ; Lipid Metabolism ; genetics ; Liver ; metabolism ; Male ; Oligonucleotide Array Sequence Analysis ; Pseudomonas Infections ; metabolism ; Rats ; Rats, Wistar ; Sepsis ; etiology ; metabolism

Glomerulonephritis associated with visceral abscess is being increasingly recognized. The association of glomerulonephritis with visceral suppuration in the absence of endocarditis was first described by Whitworth and associates. Abscesses were most frequently located in the respiratory tract but have been reported at numerous other sites, including appendix, uterus, aorto-femoral bypass graft and cutaneous wound. This report documents the apparently rare occurrence of glomerulonephritis with acute renal failure in association with pyogenic liver abscess. The need for awareness of glomerulonephritis as a cause of acute renal failure in pyogenic liver abscess is highlighted.
Aged ; Antibiotics/administration & dosage ; Biopsy, Needle ; Case Report ; Drainage/methods ; Follow-Up Studies ; Glomerulonephritis/*complications/*diagnosis/therapy ; Human ; Liver Abscess/*complications/*diagnosis/therapy ; Male ; Pseudomonas Infections/*complications/*diagnosis/therapy ; Tomography, X-Ray Computed ; Treatment Outcome

Infectious scleritis by Pseudomonas aeruginosa is a well-known vision-threatening disease. In particular, scleral trauma following pterygium surgery may increase the risk of sclera inflammation. Surgical debridement and repair is necessary in patients who do not respond to medical treatments, such as topical and intravenous antibiotics. We reports herein the effectiveness of an autologous perichondrium conchal cartilage graft for infectious scleritis caused by Pseudomonas aeruginosa. This procedure was performed on four eyes of four patients with infectious scleritis who had previously undergone pterygium surgery at Gyeongsang National University Hospital (GNUH), Jinju, Korea from December 2011 to May 2012. Pseudomonas aeruginosa was identified in cultures of necrotic scleral lesion before surgery. The conchal cartilage perichondrium graft was transplanted, and a conjunctival flap was created on the scleral lesion. The autologous perichondrium conchal cartilage graft was successful and visual outcome was stable in all patients, with no reports of graft failure or infection recurrence. In conclusion, autologous perichondrium conchal cartilage graft may be effective in surgical management of Pseudomonal infectious scleritis when non-surgical medical treatment is ineffective. Further studies in larger, diverse populations are warranted to establish the effectiveness of the procedure.
Anti-Bacterial Agents/therapeutic use ; Autografts ; Cartilage/surgery ; Communicable Diseases ; Debridement ; Eye Infections, Bacterial/etiology/*therapy ; Female ; Humans ; Ophthalmologic Surgical Procedures ; Postoperative Complications ; Pseudomonas Infections/microbiology/*therapy ; Pseudomonas aeruginosa/*isolation & purification ; Pterygium/surgery ; Republic of Korea ; Sclera/*surgery/transplantation ; Scleritis/microbiology/*therapy ; Surgical Wound Infection/microbiology/*therapy ; Transplantation, Autologous ; Treatment Outcome