Humans ; Proteoglycans ; Solitary Fibrous Tumors/surgery*

No abstract available.
Glomerular Basement Membrane* ; Heparan Sulfate Proteoglycans* ; Heparitin Sulfate*

PURPOSE: To determine the viability of human chondrocytes within refrigerated articular cartilage stored under conditions currently used clinically. MATERIALS AND METHODS: Osteochondral sections of human ankle taluses were stored at 4 degrees C in DMEM media for 1 to 42 days. Articular cartilage was harvested and evaluated for histologic changes and proteoglycan synthesis. RESULTS: By day 7, markedly decreased proteoglycan synthesis was observed. After 21 days, synthetic activity was virtually undetectable. Histologic specimens demonstrated chondrocyte death of a half of the cells from the superficial layer at day 7. Within 21 days, significant chondrocyte death was seen. CONCLUSION: These results suggest that proteoglycan synthetic activity and chondrocyte viability are markedly decreased in articular cartilage after cold storage for longer than 7 days.
Ankle ; Cartilage, Articular* ; Chondrocytes ; Humans* ; Proteoglycans ; Refrigeration ; Talus

STUDY DESIGN: A retrospective study was performed in patients who had undergone any operation with removal of lumbar intervertebral disc at Chungnam National University Hospital. OBJECTIVES: To evaluate relationship among magnetic resonance image, electron microscopic findings, light microscopic findings and clinical symptoms in degenerated intervertebral disc. SUMMARY OF LITERATURE REVIEW: Degenerative changes and disc herniations in the intervertebral disc have been shown to be accompanied by changes in the water and proteoglycan content of the tissue. MATERIALS AND METHODS: Our study followed by any operation with removal of intervertebral disc was carried out on 60 patients at Chungnam National University Hospital from January 1998 to December 1999. In radiographic evaluation we used a criteria from Frymoyer. In clinical evaluation we classified clinical symptom according to scale of Kirkaldy-Willis. And we classified of electron microscopic findings into five grades according to degrees of denudation of proteoglycan from hyalunonic acid. In light microscopic findings, we classified by cell nest formation, noevascularization and amount of muccopolysaccharide. RESULTS: In radiologic evaluation there were 11 cases in grade III, 28 cases in grade IV, and 21 cases in grade V. There were no grade I, II in our study. In clinical symptom, there were 20 cases in Good, 18 cases in Fair, and 22 cases in Poor. In electron micro-scopic findings, there were 4 cases in grade 3, 35 cases in grade 4, and 21 cases in grade 5. There were no grade I, II in this study. There was a relationship between magnetic resonance image and electron microscopic findings and clinical symptom (p<0.05). CONCLUSION Our study of electron microscopic findings of degenerated intervertebral disc may be a help to understand of pathogenesis of disc prolapse.
Chungcheongnam-do ; Humans ; Intervertebral Disc* ; Prolapse ; Proteoglycans ; Retrospective Studies

Recombinant protein SMB(PRG4) containing two Somatomedin B domains and a small amount of glycosylation of repetitive sequences of proteoglycan 4 was cloned according to PGR4 gene polymorphism. Mature purification process was established and recombinant protein SMB(PRG4), with high-level expression was purified. By using size-exclusion chromatogaraphy and dynamic light scattering, we found that the recombinant protein self-aggregate to dimeric form. Structure prediction and non-reducing electrophoresis revealed that SMB(PRG4), was a non-covalently bonded dimer.
Glycosylation ; Protein Multimerization ; Proteoglycans ; chemistry ; Recombinant Proteins ; chemistry ; Somatomedins ; chemistry

PURPOSE: The aim of this study is to determine whether degeneration severities of meniscus assessed using magnetic resonance imaging (MRI) would well estimate those assessed using histological and biochemical examinations. MATERIALS AND METHODS: Seven lateral menisci from knees with osteoarthritis undergoing total knee arthroplasty (study group) and five from normal controls (control group) were examined for this study. Degeneration severities of the menisci were graded using MRI, histologic and biochemical examinations of the menisci were then performed. Comparative analyses of MRI grading and results of histological/biochemical examinations of the menisci were performed in each group. In addition, comparative analyses of histological/biochemical conditions were performed between specimens of the study group and the control group showing grade 0 on MRI. RESULTS: All specimens from the control group showed grade 0 on MRI and their histology was also grade 0. In addition, no significant differences in biochemical results were observed among the specimens of the control group. In the lateral meniscus from the study group showing degeneration on MRI it was found that the water and proteoglycan contents increased with increasing grade of degeneration whereas the collagen content decreased. The meniscus specimens of the control group and the study group showing grade 0 on MRI had similar histologic findings but had different biochemical properties. The grade I, II degenerations on MRI were not well matched with the histologic findings in the study group. CONCLUSION: Severities of meniscus degeneration on MRI did not well reflect the histologic findings of the meniscus. This finding may be due to the water content of the meniscus. The factors of the high signal intensity of the degenerated lateral meniscus on MRI may be due to the decreased component of collagen and increased proteoglycan. Our findings suggested that caution should be taken when the severities of meniscus degeneration on MRI are attributed to histologic severities of degenerated meniscus.
Arthroplasty ; Collagen ; Knee ; Magnetic Resonance Imaging* ; Menisci, Tibial ; Osteoarthritis ; Proteoglycans

I have examined the effects of a growth factor, transforming growth factor(TGF)-B 1, on the rates of proteoglycan synthesis, aggregation potenital, and phenotypic expression of proteoglycans from human cervical intervertebral discs maintained in a cell culture system. A cell culture system for transitional and nuclear regions of degenerated human cervical intervertebral disc disc was devised to assess the biosynthetic response, assayed by 35S-sulfate incorporation into proteoglycan and protein synthesis assayed by 35S-methionine incorporation. And I had the data as the TGF-B 1 has an effect on the proteoglycan synthesis in quantitative and qualitative analysis in the cell culture system of the human intervertebral disc. TGF-B 1 may be used as a therapeutic alternative to degenerated disc disease.
Cell Culture Techniques ; Humans ; Intervertebral Disc ; Proteoglycans ; Transforming Growth Factors

Collagen ; Drug Combinations ; Durapatite ; Endothelial Cells ; Humans ; Laminin ; Proteoglycans

OBJECTIVE: Aberrant expression of the cell surface proteoglycan, syndecan-1, is found in many malignancies. The current study describes the immunohistochemical study of syndecan-1 expression in normal, hyperplastic, and malignant endometrial tissues for evaluation of application as a parameter of cancer progression in patients with endometrial hyperplasia. METHODS: Immunohistochemical staining of syndecan-1 was performed in 101 formalin fixed, paraffin embedded sections of normal, hyperplastic, and malignant endometrial tissues. We analyzed specimens from patients with normal endometrium (NE, N=10) as controls, and those of simple hyperplasia (SH, N=20), complex hyperplasia without atypia (CH, N=20), atypical hyperplasia (AH, N=20), and endometrial cancer (EC, N=31). RESULTS: The mean rank of expression scores based on the frequency of syndecan-1 staining were 31.6, 20.5, 52.9, 72.1, and 62.1 for NE, SH, CH, AH and EC, respectively (p<0.001). Syndecan-1 expression was significantly greater in CH (p<0.001) or AH (p<0.001) than in SH, and significantly greater in AH compared to CH (p=0.028). Syndecan-1 is more frequently expressed in CH (p=0.042), AH (p<0.001), or EC (p=0.002) than in NE. Syndecan-1 expression did not differ significantly between NE and SH (p=0.248). CONCLUSION: Syndecan-1 expression appears to be useful as a predictive indicator in endometrial hyperplasia.
Endometrial Hyperplasia ; Endometrial Neoplasms ; Endometrium ; Female ; Formaldehyde ; Humans ; Hyperplasia ; Paraffin ; Proteoglycans ; Syndecan-1

Congenital auricular deformities such as lop ear, cup ear, prominent ear, and Stahl's ear can be corrected nonsurgically in the early neonatal period(first week after birth) obtaining satisfactory results. Auricular cartilage consists of chondrocytes and intercellular materials that are mainly assembled from collagen, elastin, and a proteoglycan aggergate. Cartilageelasticity is dependent upon the concentration of the proteoglycan aggregate. Hyaluronic acid, which is a constituent of proteoglycan aggregate and is increased by estrogens, has an important play on disconnecting the proteoglycan aggregate. So the increased estrogen results in the lack of elasticity. The circulating levels of free estradiol are higher during the first 72 hours after birth and decreases thereafter, so the ear is soft and easily malleable in this early neonatal period. In this study, we have applied aluminium splint (90 degree-angulated, 180 degree-angulated, and rolled) on rabbit ear. All of the rabbit was divided into 3 groups containing 3 types of deformities. In group 1, estrogen was applied to the deformed ear with regional injection to the perichondrium of auricular cartilage. In group 2. estrogen was applied topically with ointment. In group 3, as control group, saline was injected to the perichondrium. Four weeks later, in group 2, the shape of the deformed rabbit ear was maintained, and did not return to its original shape even after splint removal. From these results, we concluded that estrogen ointment could be tried as adjunctive therapeutic modalities to the nonsurgical treatment of the congenital auricular deformities.
Chondrocytes ; Collagen ; Congenital Abnormalities ; Ear Cartilage ; Ear* ; Elasticity ; Elastin ; Estradiol ; Estrogens* ; Hyaluronic Acid ; Parturition ; Proteoglycans ; Splints