1.Methylation Status of PTPL1 Gene in Non-Hodgkin's Lymphoma Cells.
Wen-Ming WANG ; Jing WANG ; Hong-Mei JING
Journal of Experimental Hematology 2015;23(6):1596-1600
OBJECTIVETo investigate the methylation status of PTPL1 in Hut78, Maver, Z138, CA46, Raji and Jurkat cell lines, and the reversing effect of 5-Aza on expression of PTPL1 mRNA.
METHODSThe Hut78, Maver, Z138, CA46, Raji and Jurkat cell lines were cultured in vitro, the MS-PCR was used to detect the methylation status of PTPL1 gene and RT-PCR was used to detect the expression of PTPL1 mRNA, the CCK-8 method was used to determine the cell proliferation, the siRNA-mediated silencing of PTPL1 was used to clarify the role of PTPL1 in lymphoma cell lines.
RESULTSPTPL1 gene promoter was non-methylated in Hut78, Maver and Z138 cells, and was hyper-methylated in Raji, CA46 and Jurkat cells; the different levels of PTPL1 mRNA expression were in Hut78, Maver and Z138; the re-expression of PTPL1 mRNA resulted from hyper-methylation was found in Raji, CA46 and Jurkat cells. The 5-Aza treatment could inhibit the prolifenation of Raji, CA46 and Jurkat cell lines and induce re-expression of PTPL1 mRNA. After PTPL1 was interfered by siRNA, the growth of lymphoma cells was promoted.
CONCLUSIONThe PTPL1 gene silencing induced by hyper-methylation may be an important factor in the occurrence and development of non-Hodgkin lymphoma. The methylation of PTPL1 gene may be used as a possible molecular marker for diagnosis and treatment targets.
Cell Proliferation ; DNA Methylation ; Gene Silencing ; Humans ; Jurkat Cells ; Lymphoma, Non-Hodgkin ; Protein Tyrosine Phosphatase, Non-Receptor Type 13
2.Expression and Significance of PTPL1 in Hematological Malignancies.
Wen-Ming WANG ; Jing WANG ; Hong-Mei JING
Journal of Experimental Hematology 2014;22(6):1744-1747
PTPL1 is a protein with a predicted MW of 270 kD, and plays a major role in many cellular functions, including cell survival, proliferation, differentiation and motility. Evidence has demonstrated that PTPL1 is associated with tumor. Although many conflicting results suggested that PTPL1 has two contradictory effects (supressing or promoting ) on tumor, the real effect depends on the involved substrate and the cellular context. Expression of PTPL1 is low in lymphoma, while it is high in myeloid leukemia. PTPL1 has been regarded as a tumor suppressor in lymphoma, the methylation of PTPL1 promoter leads to gene expression reduced or disappeared, playing a lymphoma tumor suppressor role. This review focuses on PTPL1 domain and its interacting proteins, the relationship between PTPL1 and hematological malignancies.
Cell Movement
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Cell Survival
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Genes, Tumor Suppressor
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Hematologic Neoplasms
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genetics
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metabolism
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pathology
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Humans
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Protein Tyrosine Phosphatase, Non-Receptor Type 13
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genetics
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metabolism
3.Expression of Ref-1 and FAP-1 mRNA in hypoxic-ischemic injury in neonatal rat.
Chun DENG ; Chun-bao GUO ; Jia-lin YU ; Shi-xiao WU ; Yi TAN
Chinese Journal of Pediatrics 2003;41(4):297-299
Animals
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Animals, Newborn
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Brain
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blood supply
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metabolism
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pathology
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Carrier Proteins
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genetics
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DNA-(Apurinic or Apyrimidinic Site) Lyase
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genetics
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Gene Expression
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Hypoxia-Ischemia, Brain
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genetics
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pathology
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In Situ Hybridization
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Protein Tyrosine Phosphatase, Non-Receptor Type 13
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Protein Tyrosine Phosphatases
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genetics
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RNA, Messenger
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genetics
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metabolism
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Rats
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Rats, Wistar