1.Cloning and expression analysis of protein kinase C gene from Whitmania pigra.
Pan-Pan CHEN ; Qiao-Sheng GUO ; Hong-Zhuan SHI ; Li-Yuan GUO ; Hui-Ning ZHANG
China Journal of Chinese Materia Medica 2021;46(3):599-604
Protein kinase C(PKC) is a type of protein kinase widely involved in cell proliferation and development, but the developmental mechanism in the gonads of androgynous animals is still unclear. In order to explore the role of protein kinase C in the development of Whitmania pigra germ cells, the Wh. pigra PKC(Wp-PKC) gene was cloned, bioinformatics analysis was conducted, and fluorescent quantitative PCR was used to analyze the expression of female and male gonads. The results showed that:(1)The cloned Wp-PKC had a full length of 2 580 bp, a relative molecular weight of 76 555.19, and contains an open reading frame encoding 670 amino acids, Wp-PKC was closely related to Danio rerio PKC-α and rat PKC-γ. The similarity of amino acid sequence was 55% and 58%.(2)The protein encoded by Wp-PKC had no signal peptide and was a hydrophilic protein. The secondary structure is mainly composed of random coils, α-helices, extended chains, folds and folds, with the largest proportion of random coils and α-helices. Wp-PKC protein does not contain a transmembrane domain. Multiple sequence alignment and domain prediction analysis show that Wp-PKC contains 4 conserved domains of classical protein kinase C.(3)Fluorescence quantitative results showed that the expression of Wp-PKC in Wh. pigra gonads was positively correlated with the development of germ cells, and the expression in male gonads was significantly higher than that in female gonads. In summary, Wp-PKC is a classic PKC, and Wp-PKC may promote the development of Wh. pigra, especially the development of male gonads, and provide references for further research on the developmental mechanisms of Wh. pigra.
Animals
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Cloning, Molecular
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Female
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Gonads
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Leeches/genetics*
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Male
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Ovary
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Protein Kinase C/genetics*
;
Rats
2.Functional analysis of the single nucleotide polymorphisms in the PRKCZ gene.
Hong-xia SUN ; Wei-nan DU ; Yun-feng LI ; Jin ZUO ; Guo-dong WU ; Yan SHEN ; Bo-qin QIANG ; Zhi-jian YAO ; Jian-mei HANG ; Hui-yuan LUO ; Heng WANG ; Wei HUANG ; Zhu CHEN ; Fu-de FANG
Acta Academiae Medicinae Sinicae 2002;24(5):471-473
OBJECTIVETo study the function of 5 single nucleotide polymorphisms (SNPs) of the PRKCZ gene, a susceptibility gene for type 2 diabetes in Han population of North China, in the pathogenesis of the disease.
METHODSBioinformatic methods and reporter gene activity determination were used to analyze the function of the 5 SNPs.
RESULTSThe reporter gene activities of different alleles of 2 SNPs, rs427811 and rs809912, were obviously different, which implies that these 2 SNPs might be susceptibility loci of the disease.
CONCLUSIONThe PRKCZ gene is further confirmed to be a susceptibility gene for type 2 diabetes in Han population of North China. Two SNPs in the gene play a role in the pathogenesis of the disease by affecting the expression level of PRKCZ gene.
Alleles ; Asian Continental Ancestry Group ; Diabetes Mellitus, Type 2 ; genetics ; Ethnic Groups ; Genetic Predisposition to Disease ; Humans ; Polymorphism, Single Nucleotide ; Protein Kinase C ; genetics ; Protein Kinase C-delta
3.Effects of overexpression of PEMT2 on expression and translocation of different PKC isoforms in rat hepatoma cells.
Ya-li LI ; Ke-li MA ; Wei ZOU ; Quan XIA ; Zhao-chun CUI
Chinese Journal of Hepatology 2005;13(9):678-681
OBJECTIVETo explore the mechanism of cell proliferation inhibition by transfecting phosphatidylethanolamine N-methyltransferase 2 gene (PEMT2).
METHODSThe expression and translocation of different isoforms of protein kinase C (PKC) in cells were observed with immunocytochemistry and Western blot techniques. The content of diacylglycerol (DAG) was analyzed with high performance thin layer chromatography (HPTLC) technique.
RESULTSTransfection of PEMT2 can inhibit the expression of cPKC alpha, but obviously promotes the expression and translocation from cytosol to plasma membrane of cPKC beta2. At the same time, the content of DAG was decreased in the transfected cells. Expression and translocation of other PKC isoforms were not changed by PEMT2 transfection.
CONCLUSIONEffects of overexpression of PEMT2 on the expression and translocation of different PKC isoforms might be related to the mechanism of cell proliferation inhibition and apoptosis induced by transfecting PEMT2.
Animals ; Liver Neoplasms, Experimental ; enzymology ; pathology ; Phosphatidylethanolamine N-Methyltransferase ; biosynthesis ; genetics ; Protein Isoforms ; Protein Kinase C ; biosynthesis ; genetics ; Rats ; Transfection
4.Protein Kinase C Controls the Excitability of Cortical Pyramidal Neurons by Regulating Kv2.2 Channel Activity.
Zhaoyang LI ; Wenhao DONG ; Xinyuan ZHANG ; Jun-Mei LU ; Yan-Ai MEI ; Changlong HU
Neuroscience Bulletin 2022;38(2):135-148
The family of voltage-gated potassium Kv2 channels consists of the Kv2.1 and Kv2.2 subtypes. Kv2.1 is constitutively highly phosphorylated in neurons and its function relies on its phosphorylation state. Whether the function of Kv2.2 is also dependent on its phosphorylation state remains unknown. Here, we investigated whether Kv2.2 channels can be phosphorylated by protein kinase C (PKC) and examined the effects of PKC-induced phosphorylation on their activity and function. Activation of PKC inhibited Kv2.2 currents and altered their steady-state activation in HEK293 cells. Point mutations and specific antibodies against phosphorylated S481 or S488 demonstrated the importance of these residues for the PKC-dependent modulation of Kv2.2. In layer II pyramidal neurons in cortical slices, activation of PKC similarly regulated native Kv2.2 channels and simultaneously reduced the frequency of action potentials. In conclusion, this study provides the first evidence to our knowledge that PKC-induced phosphorylation of the Kv2.2 channel controls the excitability of cortical pyramidal neurons.
Action Potentials
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HEK293 Cells
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Humans
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Protein Kinase C/metabolism*
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Pyramidal Cells/enzymology*
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Shab Potassium Channels/genetics*
5.Modulating protein kinase D1 signal transduction.
Wei ZHANG ; Jian XU ; Chang-ku JIA ; Shu-sen ZHENG
Chinese Medical Journal 2009;122(15):1816-1820
6.Association between mannose-binding lectin 2 gene and protein kinase C-beta 1 gene polymorphisms and type 2 diabetic macrovascular complications in northern Chinese Han population.
Na-na ZHANG ; Li YAO ; Mao-qiang ZHUANG ; Guo-chang WANG ; Tian-tian CHEN ; Ya-jun YANG ; Juan ZHANG ; Ming LV ; Li JIN
Chinese Journal of Medical Genetics 2012;29(6):709-714
OBJECTIVETo assess the association between single nucleotide polymorphisms (SNPs) of mannose-binding lectin 2 gene (MBL2) (rs1800450, rs1800451 and rs11003125) and protein kinase C-beta 1 gene (PRKC beta 1) (rs3700106, rs2575390) with diabetic macroangiopathy in northern Chinese Han population.
METHODSThe samples have included 318 type 2 diabetes mellitus (T2DM) patients and 448 normoglycemic controls. The five SNPs were determined by a Multiplex SnaPshot method. Biochemical indices such as fasting plasma-glucose, triglyceride and total cholesterol were also measured. Linkage disequilibrium and haplotype analysis were carried out for all samples using Haploview 4.2. Additive model was applied to assess the effect of interaction between SNPs and environment factors on macrovascular complications.
RESULTSGenotypic frequencies of rs11003125 have differed significantly between the controls and patients with coronary heart disease and peripheral vascular disease (P=0.024 and 0.004, respectively). The allele frequency of rs11003125 was also statistically significant between the two groups (P=0.014 and 0.001, respectively). Compared with patients without macrovascular complications, the allele frequency of rs11003125 was significantly different in patients with peripheral vascular disease (P=0.031). No significant differences were found between the distribution of the genotype frequency and allele frequencies of other variants. Haplotype analysis indicated that, compared with controls and patients without macrovascular complications, individuals with G allele of rs1800450 and C allele of rs11003125 had a higher risk for macrovascular complications.
CONCLUSIONThe rs11003125 polymorphism located in the promoter region of MBL2 gene is associated with macrovascular complications of T2DM in northern Chinese Han population. G allele of rs1800450 and C allele of rs11003125 may be risk factors for macrovascular complications. There were additive interactive effects for rs11003125 polymorphism (GC+CC) and hypertension, diabetic nephropathy, diabetic neuropathy and diabetic retinopathy on macrovascular complications.
Alleles ; China ; ethnology ; Diabetes Mellitus, Type 2 ; ethnology ; genetics ; Diabetic Angiopathies ; ethnology ; genetics ; Gene Frequency ; Genetic Predisposition to Disease ; Genotype ; Humans ; Mannose-Binding Lectin ; genetics ; Polymorphism, Single Nucleotide ; Promoter Regions, Genetic ; Protein Kinase C ; genetics ; Protein Kinase C beta
7.Linkage disequilibrium analysis of the single nucleotide polymorphisms in the PRKCZ gene.
Hong-xia SUN ; Wei-nan DU ; Yun-feng LI ; Jin ZUO ; Guo-dong WU ; Yan SHEN ; Bo-qin QIANG ; Zhi-jian YAO ; Jian-mei HANG ; Hui-yuan LUO ; Heng WANG ; Wei HUANG ; Zhu CHEN ; Fu-de FANG
Acta Academiae Medicinae Sinicae 2002;24(5):474-480
OBJECTIVETo search for the disease-associated haplotype in the PRKCZ gene, a susceptibility gene for type 2 diabetes in Han population of North China, by case-control study and linkage disequilibrium (LD) analysis using single nucleotide polymorphisms (SNPs).
METHODSSNPs located in the PRKCZ gene were chosen from public SNP domain by bioinformatic methods and single base extension (SBE) method was used to genotype the loci in 173 sporadic type 2 diabetes patients and 152 normal individuals to perform case-control study and LD analysis. Haplotype block were constructed in these populations.
RESULTSSeveral SNPs in the PRKCZ gene were found to be associated with the disease. The SNPs formed different haplotype block pattern in case and control groups. The frequencies of the haplotypes formed by 5 SNPs were statistically different between the two groups.
CONCLUSIONThe haplotype formed by 5 SNPs in the PRKCZ gene may be associated with type 2 diabetes in Han population of China, which is confirmed from statistics to be a susceptibility gene for the disease.
Alleles ; Asian Continental Ancestry Group ; Case-Control Studies ; Diabetes Mellitus, Type 2 ; genetics ; Ethnic Groups ; Genetic Predisposition to Disease ; Haplotypes ; Humans ; Linkage Disequilibrium ; Polymorphism, Single Nucleotide ; Protein Kinase C ; genetics ; Protein Kinase C-delta
8.Calcitonin induces connective tissue growth factor through ERK1/2 signaling in renal tubular cells.
Misa NAKAMURA ; Takashi OZAKI ; Aiko ISHII ; Masayoshi KONISHI ; Yuji TSUBOTA ; Toru FURUI ; Hayato TSUDA ; Ichiro MORI ; Kiichiro OTA ; Kennichi KAKUDO
Experimental & Molecular Medicine 2009;41(5):307-314
Calcitonin (CT), a polypeptide hormone, plays important roles in a variety of physiological processes. CT has been used clinically to treat osteoporosis and humoral hypercalcemia of malignancy. In order to clarify the pharmacological effects of CT in the kidney, we identified potential downstream genes induced by CT in the renal cells. Using a cDNA subtraction hybridization method, we identified connective tissue growth factor (CTGF) as a CT-induced gene in the porcine renal cell line, LLC-PK1. Furthermore, we found that CT-mediated induction of the gene was not inhibited by cycloheximide, which suggests that CTGF gene was not induced by an increased synthesis of regulating proteins. Therefore, CTGF is an immediate early gene. We further demonstrated that the regulation of CTGF gene expression by CT involved the ERK1/2 pathway, because PD98059, a MEK1 inhibitor, partially inhibited the mRNA expression of CTGF induced by CT. CT-induced CTGF protein expression was also observed in vivo. Our present findings suggest that CT induces the transcription of CTGF through ERK1/2 phosphorylation. We also identified twelve other genes induced by CT that, like CTGF, were related to wound healing. These results suggest that CT may have an effect on renal differentiation and wound healing in the kidney.
Animals
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Calcitonin/*pharmacology
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Cell Line
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Connective Tissue Growth Factor/*genetics/metabolism
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Female
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Kidney Tubules, Proximal/*enzymology/metabolism
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*MAP Kinase Signaling System
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Mice
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Mice, Inbred BALB C
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Mitogen-Activated Protein Kinase 1/*metabolism
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Mitogen-Activated Protein Kinase 3/*metabolism
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Phosphorylation
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Swine
9.Calcitonin induces connective tissue growth factor through ERK1/2 signaling in renal tubular cells.
Misa NAKAMURA ; Takashi OZAKI ; Aiko ISHII ; Masayoshi KONISHI ; Yuji TSUBOTA ; Toru FURUI ; Hayato TSUDA ; Ichiro MORI ; Kiichiro OTA ; Kennichi KAKUDO
Experimental & Molecular Medicine 2009;41(5):307-314
Calcitonin (CT), a polypeptide hormone, plays important roles in a variety of physiological processes. CT has been used clinically to treat osteoporosis and humoral hypercalcemia of malignancy. In order to clarify the pharmacological effects of CT in the kidney, we identified potential downstream genes induced by CT in the renal cells. Using a cDNA subtraction hybridization method, we identified connective tissue growth factor (CTGF) as a CT-induced gene in the porcine renal cell line, LLC-PK1. Furthermore, we found that CT-mediated induction of the gene was not inhibited by cycloheximide, which suggests that CTGF gene was not induced by an increased synthesis of regulating proteins. Therefore, CTGF is an immediate early gene. We further demonstrated that the regulation of CTGF gene expression by CT involved the ERK1/2 pathway, because PD98059, a MEK1 inhibitor, partially inhibited the mRNA expression of CTGF induced by CT. CT-induced CTGF protein expression was also observed in vivo. Our present findings suggest that CT induces the transcription of CTGF through ERK1/2 phosphorylation. We also identified twelve other genes induced by CT that, like CTGF, were related to wound healing. These results suggest that CT may have an effect on renal differentiation and wound healing in the kidney.
Animals
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Calcitonin/*pharmacology
;
Cell Line
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Connective Tissue Growth Factor/*genetics/metabolism
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Female
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Kidney Tubules, Proximal/*enzymology/metabolism
;
*MAP Kinase Signaling System
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Mice
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Mice, Inbred BALB C
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Mitogen-Activated Protein Kinase 1/*metabolism
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Mitogen-Activated Protein Kinase 3/*metabolism
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Phosphorylation
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Swine
10.Effects of aquaporin 4 deficiency on the expression of spinal PKCα, PKCγ and c-Fos in naloxone-precipitated morphine withdrawal mice.
Meng-Ling CHEN ; Feng BAO ; Yu-Qiu ZHANG ; Zhi-Qi ZHAO
Acta Physiologica Sinica 2012;64(4):365-371
The previous study indicated that aquaporin 4 (AQP4) deficiency attenuated opioid physical dependence. However, the underlying mechanism remains unknown. In the present study, the effects of AQP4 deficiency on the expression of three factors, protein kinase C (PKC) α, PKCγ and c-Fos in the spinal cord, which are known to be concerned with spinal neuronal sensitization and opiate dependence, were investigated in AQP4 knockout mice using Western blotting analysis. It was observed that AQP4 deficiency reduced the score of naloxone-precipitated abstinent jumping after repeated morphine administration compared with wild-type (P < 0.001). Meanwhile, the protein levels of PKCα and c-Fos in the spinal cord of AQP4 knockout mice were significantly higher than those in the wild-type mice; while the expression of PKCγ was decreased remarkably by AQP4 knockout during the withdrawal (P < 0.01). These data suggest that AQP4 deficiency-attenuated morphine withdrawal responses may be partially attributed to the changes in the spinal expression of PKCα, PKCγ or c-Fos.
Analgesics, Opioid
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pharmacology
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Animals
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Aquaporin 4
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deficiency
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genetics
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Mice
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Mice, Knockout
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Morphine
;
pharmacology
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Naloxone
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pharmacology
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Protein Kinase C
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metabolism
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Protein Kinase C-alpha
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metabolism
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Spinal Cord
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metabolism
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Substance Withdrawal Syndrome
;
metabolism