1.The Protein Oxidation-Stimulating Activities in the Uterine Venous, Umbilical Venous, and Umbilical Arterial Plasma of Preeclampsia.
Jae Hoon KIM ; Yoon Ha KIM ; Cheol Hong KIM ; Moon Kyoung CHO ; Ki Min KIM ; Soo Young PARK ; Tae Bok SONG ; Bong Whan AHN ; Sung Yeul YANG
Korean Journal of Obstetrics and Gynecology 2005;48(7):1635-1644
OBJECTIVE: This study was performed to compare the prooxidative activity stimulating the protein carbonyl formation by 3rd generation cephalosporin (moxalactam) and amoxacillin in the uterine venous, umbilical venous, and umbilical arterial plasma of preeclampsia with that of normal pregnancy. METHODS: Lipid peroxide levels in the uterine venous, umbilical venous, and umbilical arterial plasma of normal pregnancy (n=16) and preeclampsia (n=16) were measured by thiobarbituric acid reaction. The basal protein carbonyl contents in the uterine venous, umbilical venous, and umbilical arterial plasma of normal pregnancy (n=16) and preeclampsia (n=16) were determined by the 2,4-dinitrophenylhydrazine (DNPH) method. After plasma of them were mixed and incubated up to 5 hours with 0.2 mL of 1 mM moxalactam or amoxacillin, the protein carbonyl contents in them were measured by DNPH. RESULTS: Lipid peroxide levels in the uterine venous plasma, umbilical venous plasma, and umbilical arterial plasma of preeclampsia were significantly higher than those of normal pregnancy (3.11+/-1.21 vs. 2.18+/-1.16 nmol/mg protein, p<0.05, 5.85+/-1.67 vs. 3.79+/-1.66 nmol/ mg protein, p<0.01, 6.00+/-1.91 vs. 4.99+/-1.78 nmol/mg protein, p<0.01). Protein carbonyls formation by moxalactam in the uterine venous plasma, umbilical venous plasma, and umbilical arterial plasma of preeclampsia were signigicant higher than those of normal pregnancy (19.69+/-8.43 vs. 10.84+/-3.00 nmol/mg protein, p<0.01, 18.94+/-6.96 vs. 10.63+/-1.81 nmol/mg protein, p<0.01, 14.62+/-5.77 vs. 11.21+/-2.08 nmol/mg protein, p<0.05). There were significant positive correlations between lipid peroxide and moxalactam-induced protein carbonyls levels of the uterine venous plasma, umbilical venous plasma, and umbilical arterial plasma (p<0.01). CONCLUSION: These results suggest that increase in the prooxidative activity stimulating the oxidative modification of proteins in utero-placental unit may be involved in the pathogenesis of preeclampsia.
Moxalactam
;
Plasma*
;
Pre-Eclampsia*
;
Pregnancy
;
Protein Carbonylation
2.Increased Prooxidative Activity Stimulating the Oxidative Modification of Proteins in the Umbilical Venous Plasma and Placenta of Preeclampsia.
Eun Mi KIM ; Yoon Ha KIM ; Bong Whan AHN ; Sung Yeul YANG ; Cheol Hong KIM ; Moon Kyoung CHO ; Seok Mo KIM ; Tae Bok SONG
Korean Journal of Perinatology 2004;15(1):27-33
OBJECTIVE: This study was performed to compare the prooxidative activity stimulating the protein carbonyl formation by cephalosporins in the umbilical venous and placenta of preeclampsia with that of normal pregnancy. METHODS: Lipid peroxide levels in the umbilical venous plasma and placental tissue homogenates of normal pregnancy (n=12) and preeclampsia (n=12) were measured by thiobarbituric acid reaction. The basal protein carbonyl contents in the umbilical venous plasma and placental tissue homogenates of normal pregnancy (n=12) and preeclampsia (n=12) were determined by the 2,4-dinitrophenylhydrazine (DNPH) method. After samples of them were mixed and incubated up to 5 hours with 0.2 mL of 1 mM moxalactam or cephalothin, the protein carbonyl contents in them were measured by DNPH. RESULTS: Protein carbonyls formation by moxalactam and cephalothin in the umbilical venous plasma and of women with preeclampsia were significantly higher than that of women with normal pregnancy (8.5+/-2.0 vs. 6.6+/-1.4 nmol/mg protein, p<0.05, 7.6+/-1.6 vs. 6.2+/-1.2 nmol/mg protein, p<0.05). Protein carbonyls formation by moxalactam and cephalothin in the placental tissue homogenates of women with preeclampsia were significantly higher than that of women with normal pregnancy (17.6+/-5.3 vs. 13.0+/-4.2 nmol/mg protein, p<0.05, 16.1+/-5.2 vs. 12.5+/-4.4 nmol/mg protein, p<0.05). There were significant positive correlations between lipid peroxide and cephalosporins induced protein carbonyls levels of umbilical venous plasma, and placental tissue homogenates (p<0.01). CONCLUSION: These results suggest that increase in the prooxidative activity stimulating the oxidative modification of proteins in placenta may be involved in the pathogenesis of preecalmpsia.
Cephalosporins
;
Cephalothin
;
Female
;
Humans
;
Moxalactam
;
Placenta*
;
Plasma*
;
Pre-Eclampsia*
;
Pregnancy
;
Protein Carbonylation
3.Increase in the lipid peroxidation and protein oxidation in the venous plasma of women with preterm pemature rupture of membranes.
Moon Kyoung CHO ; Yoon Ha KIM ; Cheol Hong KIM ; Ki Min KIM ; Bong Whan AHN ; Sung Yeul YANG ; Jong Woon KIM ; Tae Bok SONG
Korean Journal of Obstetrics and Gynecology 2007;50(4):612-619
OBJECTIVE: This study was performed to investigate the lipid peroxide levels and the protein carbonyl groups content in the venous plasma of pregnant women with preterm premature rupture of membranes (PPROM), non-pregnant, and normal pregnant women. METHODS: Samples of venous blood were obtained from women with non pregnancy (n=20), normal pregnancy between 25 and 37 weeks gestation (n=20), and PPROM before 37 completed weeks gestation (n=20). Lipid peroxide levels in the venous plasma of women of each group were measured by thiobarbituric acid reaction. The basal, amoxacillin and moxalactam-induced protein carbonyl contents in the venous plasma of women of each group were determined by the 2,4-dinitrophenylhydrazine (DNPH) method. RESULTS: 1. Lipid peroxide levels in the venous plasma of PPROM was significantly higher than that of non-pregnant and normal pregnant women (5.66+/-0.43 vs. 3.78+/-0.24 vs. 3.56+/-0.30 nmol/mg protein, p<0.01). 2. Protein carbonyl levels in the venous plasma of PPROM was also significantly higher than that of non-pregnant and normal pregnant women (8.23+/-0.54 vs. 6.39+/-0.23 vs. 6.54+/-0.24 nmol/mg protein, p<0.01). 3. Protein carbonyls formation by moxalactam in the venous plasma of PPROM was significantly higher than that of non-pregnant and normal pregnant women (11.73+/-0.59 vs. 10.06+/-0.26 vs. 10.10+/-0.22 nmol/mg protein, p<0.05). 4. There was no significant difference in protein carbonyls formation by amoxacillin of the venous plasma of pregnant women with PPROM, non-pregnant, and normal pregnant women (5.63+/-0.41 vs. 5.81+/-0.43 vs. 5.81+/-0.39 nmol/mg protein, p>0.05). 5. There were significant positive correlations between lipid peroxide and moxalactam-induced protein carbonyls levels of the venous plasma (p<0.05). There were no significant positive correlations between lipid peroxide and amoxacillin-induced protein carbonyls levels of the venous plasma. CONCLUSION: In the venous plasma of pregnant women with PPROM, the lipid peroxidation and the protein carbonyl formation were increased. And moxalactam-induced protein carbonyl levels were increased in PPROM. These results suggest that oxydative stress was increased in pregnant women with PPROM.
Female
;
Humans
;
Lipid Peroxidation*
;
Membranes*
;
Moxalactam
;
Plasma*
;
Pregnancy
;
Pregnant Women
;
Protein Carbonylation
;
Rupture*
4.The Effect of Estrogen on Antioxidant Enzymes in Bone of Oophorectomized Rats.
Young Do KOH ; Se In OH ; Mee Sook LEE ; Chang Mo KANG ; Chung Shil KWAK ; Sang Cheol SEONG ; Sang Chul PARK
The Journal of the Korean Orthopaedic Association 1998;33(1):148-157
The purpose of this study is to evaluate an antioxidative effect of estrogen on the bone in oophorectomized rats. Thirty Sprague-Daley rats were equally divided into 3 groups; group 1 as control group with sham operation, group 2 as experimental group with oophorectomy, and group 3 as oophorectomized group treated with estrogen. Estradiol (5mg/kg BW) was administered three times per week from first to sixth week after oophorectomy. Left tibia was obtained to measure the amount of protein carbonyls as an index of oxidative stress and the activity of antioxidant enzymes. The results were as follows: trahecular bone area in proximal tihia decreased after oophorectomy, which increased in response to estrogen administration. The level of protein carbonylation in hone was not significantly different among all groups. Activity of antioxidant enzymes such ais glutathione reductase(GR), glutathione peroxidase(GP) and glutathione transferase(GST) in bone was not significantly different among all groups. However, the activity of catalase in bone markedly increased in group 3 compared with that in group 1 and group 2. In summary, bone trabecular area increased after admin- istration of estrogen. And estrogen induced the activitv of catalase, which might contrihute to prevent the oxidative damage. However, the glutathione utilizing enzymes such as GR, GP and GST were not significantly affected by estrogen status.
Animals
;
Catalase
;
Estradiol
;
Estrogens*
;
Female
;
Glutathione
;
Ovariectomy
;
Oxidative Stress
;
Protein Carbonylation
;
Rats*
;
Tibia
5.Increased protein carbonyls as markers for oxidative stress in amniotic fluid of preeclamptic women at term.
Byoung Jae KIM ; Joong Shin PARK
Korean Journal of Obstetrics and Gynecology 2007;50(10):1354-1362
OBJECTIVE: To determine if the content of protein carbonyls in amniotic fluid is elevated in preeclamptic women at term. METHODS: A case-control study was conducted to compare contents of protein carbonyls in term amniotic fluid (gestational age at amniocentesis > or = 37 wks) among women who were healthy pregnant, preeclamptic and chronic hypertensive. Protein carbonyls were measured by specific immunoassay. Nonparametric techniques were used for statistical analysis. RESULTS: 1) The median amniotic fluid protein carbonyl of preeclamptic women was significantly higher than that of normal pregnant women (median 0.663 nmol/mg, range 0.013-1.726 nmol/mg vs. median 0.395 nmol/mg, range 0.069-1.503 nmol/mg, p<.05). 2) There was no significant difference in the amniotic fluid protein carbonyl level between women with chronic hypertension and normal pregnant women (median 0.823 nmol/mg, range 0.064-1.575 nmol/mg vs. median 0.395 nmol/mg, range 0.069-1.503 nmol/mg, p=.14). 3) The median amniotic fluid protein carbonyl showed tendency to increase with severity of preeclampsia without reaching statistical significance (normal pregnancy, median 0.395 nmol/mg, range 0.069-1.503 nmol/mg; mild preeclampsia, median 0.663 nmol/mg, range 0.013-1.054 nmol/mg; severe preeclampsia, median 0.842 nmol/mg, 0.391-1.726 nmol/mg; p=.07). The median protein carbonyl in amniotic fluid of women with severe preeclampsia was significantly higher than that of normal pregnant women (median 0.842 nmol/mg, range 0.391-1.726 nmol/mg vs. median 0.395 nmol/mg, range 0.069-1.503 nmol/mg, p<.05). CONCLUSIONS: The protein carbonyls increased in amniotic fluid of women with preeclampsia, but not chronic hypertension at term.
Amniocentesis
;
Amniotic Fluid*
;
Case-Control Studies
;
Female
;
Humans
;
Hypertension
;
Immunoassay
;
Oxidative Stress*
;
Pre-Eclampsia
;
Pregnancy
;
Pregnant Women
;
Protein Carbonylation
6.Clinical features, levels of protein carbonyl in serum of obstrucitive sleep apnea syndrome with type 2 diabetes mellitus.
Liqing SU ; Haiyan CHI ; Jizhou LI ; Haijing WANG ; Changqing SUN
Journal of Clinical Otorhinolaryngology Head and Neck Surgery 2015;29(14):1275-1278
OBJECTIVE:
To explore the prevalence, clinical feature and levels of protein carbonyl (PCO) in serum of type 2 diabetes mellitus combining obstrucitive sleep apnea syndrome (OSAHS).
METHOD:
Two hundred and three patients with type 2 diabetes were taken multi lead sleep detection and their AHI, age, height, body mass index (BMI),waistline, duration of diabetes, fast blood glucose, HbA1c level and level of PCO in serum were recorded.
RESULT:
The prevalence of OSAHS was 79.2% in 203 patients, serious apnea 30.4%, moderate apnea 45.4%, mild apnea 24.2%. BMI, waistline, fast blood glucose, HbA1c level and level of PCO in serum were higher in patients complicated with OSAHS (P < 0.01). HbA1c was independently and positively correlated with patients with OSAHS risk (P < 0.05, OR 6.11). The independent correlation factors of AHI included HbA1c level,BMI, waistline, duration of diabetes and level of PCO in serum,with HbA1c as the predominant factor (P < 0.05).
CONCLUSION
The prevalence of OSAHS was higher in patients of type 2 diabetes mellitus combining (OSAHS). Moreover, in these patients poor glucose control and aggravated protein oxidative injury were observed.
Body Mass Index
;
Diabetes Mellitus, Type 2
;
blood
;
complications
;
Humans
;
Prevalence
;
Protein Carbonylation
;
Risk Factors
;
Sleep Apnea, Obstructive
;
blood
;
complications
7.The Roles of Reactive Oxygen Species Produced by Contact Allergens and Irritants in Monocyte-derived Dendritic Cells.
Dashlkhumbe BYAMBA ; Tae Gyun KIM ; Dong Hyun KIM ; Jeong Hwan JE ; Min Geol LEE
Annals of Dermatology 2010;22(3):269-278
BACKGROUND: Although reactive oxygen species (ROS) have been produced in both mouse bone marrow-derived dendritic cells (DCs) and XS-106 DCs by contact sensitizers and irritants in previous studies, the generation of ROS in human monocyte-derived DCs (MoDCs) and their role in contact hypersensitivity (CHS) has yet to be elucidated. OBJECTIVE: The purpose of this study was to determine whether contact allergens and irritants induce ROS in MoDCs and, if so, to evaluate the role of contact allergen and irritant induced-ROS in MoDCs in CHS. METHODS: Production of ROS was measured by 5-(and-6)-chloromethyl-2',7'-dichlorodihydrofluorescein diacetate (CM-H2DCFDA) assay. Surface CD86 and HLA-DR molecules were detected by flow cytometry. Protein carbonylation was detected by Western blotting. RESULTS: ROS were produced by contact allergens such as dinitrochlorobenzene (DNCB) and thimerosal and the irritant benzalkonium chloride (BKC). DNCB-induced, but not BKC-induced, ROS increased surface CD86 and HLA-DR molecules on MoDCs and induced protein carbonylation. These changes were reduced in the presence of antioxidant N-acetyl cysteine. CONCLUSION: Our results suggest that DNCB-induced ROS may be different from those induced by irritant BKC. The DNCB-induced ROS may be associated with the CHS response, because they activate surface molecules on DCs that are important for generating immune reactions.
Allergens
;
Animals
;
Benzalkonium Compounds
;
Blotting, Western
;
Cysteine
;
Dendritic Cells
;
Dermatitis, Contact
;
Dinitrochlorobenzene
;
Flow Cytometry
;
HLA-DR Antigens
;
Humans
;
Irritants
;
Mice
;
Protein Carbonylation
;
Reactive Oxygen Species
;
Thimerosal
8.Relationships among Oxidative Stress Markers, Life Style Factors and Biochemical Findings.
Yu Kyung KIM ; Duk Hee LEE ; Soo Hee JIN ; Won Gil LEE ; Kyung Eun SONG
The Korean Journal of Laboratory Medicine 2006;26(5):343-350
BACKGROUND: In many studies, oxidative stress markers have been employed to serve as a measure of a disease process or to reflect oxidative status. These oxidative stress markers must have some degree of predictive validity, but full substantiation of this relation is still lacking. This paper presents data on levels of three biomarkers, oxidized low-density lipoproteins (LDL), carbonyl, and 8-hydroxy-2'-deoxyguanosine (8-OHdG), and a number of life style factors associated with oxidative stress in healthy adults. METHODS: For 237 healthy adults aged 40-60 years, a number of life style factors, biochemical characteristics and oxidative status were evaluated. Markers of oxidative stress were measured by an ELISA method. RESULTS: Waist-hip ratio and use of vitamin supplement were associated with serum oxidized LDL (P<0.05). Body mass index and stress had a relationship (P<0.05) with protein carbonyl. Creactive protein was related to serum oxidized LDL (P<0.01). There was no correlation among three oxidative stress markers, oxidized LDL, carbonyl, and 8-OHdG. CONCLUSIONS: The oxidative stress markers used in this study could not be regarded as a general estimate of the healthy individual oxidative status. Further studies focusing on the development of biomarkers to reflect changes in the oxidative status under normal, non-pathological conditions in humans will be required.
Adult
;
Biomarkers
;
Body Mass Index
;
Deoxyguanosine
;
Enzyme-Linked Immunosorbent Assay
;
Humans
;
Life Style*
;
Lipoproteins, LDL
;
Oxidative Stress*
;
Protein Carbonylation
;
Vitamins
;
Waist-Hip Ratio
9.Changes of lipid peroxide levels and prooxidative activity stimulating the protein carbonyl formation in the amniotic fluid of women with preterm premature rupture of membranes.
Yoon Ha KIM ; Seok Mo KIM ; Cheol Hong KIM ; Moon Kyoung CHO ; Jong Woon KIM ; Woo Dae KANG ; Hye Yeon CHO ; Ju Mi PARK ; Eun Kyoung KIM ; Seo Yeon PARK ; Sung Yeul YANG ; Bong Whan AHN ; Tae Bok SONG
Korean Journal of Obstetrics and Gynecology 2009;52(2):188-196
OBJECTIVE: To investigate the lipid peroxide levels and protein carbonyls levels in the amniotic fluid of pregnant women with preterm premature rupture of membranes (PPROM). METHODS: The lipid peroxide levels in the amniotic fluid of normal pregnancy (n=20) and pregnant women with PPROM (n=20) were measured by thiobarbituric acid reaction. The protein carbonyl contents in the amniotic fluid of normal pregnancy (n=20) and pregnant women with PPROM (n=20) were determined by the 2,4-dinitrophenylhydrazine method. After amniotic fluid of them were mixed and incubated up to 5 hours with 0.2 mL of 1mM moxalactam, cefodizime, amoxacillin, erythromycin, the lipid peroxide levels and protein carbonyl contents in them were measured. RESULTS: 1. The lipid peroxide levels in the amniotic fluid of pregnant women with PPROM was significantly higher than that of normal pregnancy (9.74+/-0.48 vs. 7.20+/-0.38 nmol/mg protein, P<0.01). 2. The protein carbonyl levels in the amniotic fluid of pregnant women with PPROM was significantly higher than that of normal pregnancy (13.0+/-0.33 vs. 11.27+/-0.17 nmol/mg protein P<0.01). 3. The lipid peroxide levels and protein carbonyls formation by moxalactam in the amniotic fluid of pregnant women with PPROM was significantly higher than basal level (12.08+/-0.81 vs. 9.74+/-0.48 nmol/mg protein, 20.08+/-0.66 vs. 13.0+/-0.33 nmol/mg protein, P<0.01). 4. The lipid peroxide levels and protein carbonyls formation by cefodizime in the amniotic fluid of pregnant women with PPROM was significantly lower than basal level (5.04+/-0.33 vs. 9.74+/-0.48 nmol/mg protein, 9.76+/-0.35 vs. 13.0+/-0.33 nmol/mg protein, P<0.01). 5. There were no significant differences in the levels of lipid peroxide and protein carbonyls by amoxacillin and erythromycin in the amniotic fluid of pregnant women with PPROM between antibiotics-induced and basal levels. CONCLUSION: The lipid peroxidation and the protein carbonyls formation were increased in the amniotic fluid of pregnant women with PPROM. Antibiotics-induced lipid peroxide and protein carbonyl levels were changed in the amniotic fluid of pregnant women with PPROM. Further studies on our results may be beneficial in the selection of antibiotics for pregnant women with PPROM.
Amniotic Fluid
;
Anti-Bacterial Agents
;
Cefotaxime
;
Erythromycin
;
Female
;
Humans
;
Lipid Peroxidation
;
Membranes
;
Moxalactam
;
Phenylhydrazines
;
Pregnancy
;
Pregnant Women
;
Protein Carbonylation
;
Rupture
;
Thiobarbiturates
10.Protective Effect of Fisetin (3,7,3',4'-Tetrahydroxyflavone) against gamma-Irradiation-Induced Oxidative Stress and Cell Damage.
Mei Jing PIAO ; Ki Cheon KIM ; Sungwook CHAE ; Young Sam KEUM ; Hye Sun KIM ; Jin Won HYUN
Biomolecules & Therapeutics 2013;21(3):210-215
Ionizing radiation can induce cellular oxidative stress through the generation of reactive oxygen species, resulting in cell damage and cell death. The aim of this study was to determine whether the antioxidant effects of the flavonoid fisetin (3,7,3',4'-tetrahydroxyflavone) included the radioprotection of cells exposed to gamma-irradiation. Fisetin reduced the levels of intracellular reactive oxygen species generated by gamma-irradiation and thereby protected cells against gamma-irradiation-induced membrane lipid peroxidation, DNA damage, and protein carbonylation. In addition, fisetin maintained the viability of irradiated cells by partially inhibiting gamma-irradiation-induced apoptosis and restoring mitochondrial membrane potential. These effects suggest that the cellular protective effects of fisetin against gamma-irradiation are mainly due to its inhibition of reactive oxygen species generation.
Antioxidants
;
Apoptosis
;
Cell Death
;
DNA Damage
;
Lipid Peroxidation
;
Membrane Potential, Mitochondrial
;
Membranes
;
Oxidative Stress*
;
Protein Carbonylation
;
Radiation, Ionizing
;
Reactive Oxygen Species