No abstract available.
Biomarkers* ; Magnesium* ; Propionibacterium acnes* ; Propionibacterium*

No abstract available.
Acne Vulgaris* ; Cytosol* ; Humans ; Propionibacterium acnes* ; Propionibacterium*

No abstract available.
Biomarkers* ; Propionibacterium acnes* ; Propionibacterium* ; Vitamin D* ; Vitamins*

No abstract available.
Biomarkers* ; Propionibacterium acnes* ; Propionibacterium* ; Vitamin D* ; Vitamins*

BACKGROUND: Phototherapy has been studied as an alternative option for the treatment of acne. Propionibacterium (P.) acnes produces endogenous porphyrins as a by-product of metabolism. Light of particular wavelengths activates the porphyrins and induces a photodynamic reaction which subsequently kills the P. acnes. OBJECTIVE: The purpose of this study was to evaluate and analyze the photoinactivation efficacy of different wavelengths against P. acnes in vitro. METHODS: Using an LED lamps to emit light of variable wavelengths (370 nm, 385 nm, 395 nm, 405 nm, 470 nm, 505 nm, 590 nm, 630 nm and 880 nm), we examined the change of P. acnes colonies on agar plates after irradiation for 1, 3, 6 and 12 hours. Further investigations were also performed by irradiation on liquid medium, once, twice or three times consecutively, with an interval of 24 hours. RESULTS: Light irradiation of wavelengths 385 nm, 395 nm, 405 nm and 470 nm, was found to decrease the number of P. acnes colonies on agar plates by about 50%. In liquid medium, we observed the photoinactivation effect of light irradiation on P. acnes with the wavelengths 370 nm, 385 nm, 395 nm, 405 nm and 470 nm. However, we observed no photoinactivation effect on P. acnes with the wavelengths 505 nm, 590 nm, 630 nm or 880 nm. CONCLUSION: By in vitro testing, we have demonstrated that light irradiation of wavelengths up to a maximum of 470 nm are effective in decreasing P. acnes and therefore may be a good alternative treatment modality for acne.
Acne Vulgaris ; Agar ; Metabolism ; Phototherapy ; Porphyrins ; Propionibacterium acnes* ; Propionibacterium*

We report the cases of 3 young individuals, each of whom presented to our hospital for the evaluation and treatment of sudden onset chondrolysis. Previously, all 3 individuals had undergone arthroscopic knee procedures over a similar time at another hospital. In this paper, we review the possible problems that cause chondrolysis during or after arthroscopic procedures, such as the followings: use of thermal treatment, occult infection with Propionibacterium acnes, idiopathic or iatrogenic osteochondral injury, high temperature of fluid during arthroscopic irrigation, improperly placed implants, and the use of an intra-articular pain pump. We can exclude other causes of chondrolysis through the clinical course of the patients and surgical records and progress records. We conclude that the possible cause of chondrolysis in all cases was due to thermal injury caused by high temperatures, which decreased or blocked the irrigation fluid flow generated during arthroscopy.
Arthroscopy ; Humans ; Knee ; Knee Joint ; Propionibacterium acnes

The aim of this study was to investigate antimicrobial effect of oleanolic acid (OA), ursolic acid (UA), and sophoraflavanone G against Enterococcus faecalis and Propionibacterium acnes, which are the major causative bacteria of endodontic infections. The antimicrobial activity was evaluated by the minimal inhibitory concentration (MIC). The data showed that the OA, UA, and sophoraflavanone G had antimicrobial effect on all the strains use in the study with 16-64 microg/ml, 8-64 microg/ml, and 1-8 microg/ml of MIC values, respectively. These results indicate that OA, UA, and sophoraflavanone G could be useful in the development of antiseptic solution for washing the root canal in endodontic treatments.
Bacteria ; Dental Pulp Cavity ; Enterococcus faecalis* ; Oleanolic Acid* ; Propionibacterium acnes*

To investigate the effect of 5-aminolevulinic acid photodynamic therapy (ALA-PDT) on () biofilm. biofilms were constructed on a cell slide and treated with ALA-PDT.According to different light doses,the biofilms were divided into six groups:ALA-PDT group [ALA-PDT1 (50 J/cm),ALA-PDT2 group (100 J/cm),ALA-PDT3 group (200 J/cm)],ALA-only group (ALA group),light-only group (LED),and a negative control group (ALA-PDT-group).The biofilm structure and the ratio of the dead bacteria/live bacteria were observed using a laser confocal microscope (CLSM).Biofilm viability was measured using the XTT assay. CLSM showed that the biofilm structures of ALA group and LED group were not significantly different from that of ALA-PDT-group,whereas the biofilm structure was more seriously damaged in ALA-PDT1 group,ALA-PDT2 group,and ALA-PDT3 group than in the ALA-PDT-group.The ratios of the dead/live bacteria in ALA-PDT-group,ALA group,LED group,ALA-PDT1 group,ALA-PDT2 group,and ALA-PDT3 group were 0.350±0.033, 0.305±0.046, 0.330±0.032, 1.525±0.439, 2.293±0.148 and 3.092±0.189,respectively.ALA group(=0.003, =1.000)and LED group(=-0.025, =1.000)did not significantly differ from the ALA-PDT-group.However,the ratio of dead/live bacteria in ALA-PDT-group was significantly lower than those in ALA-PDT1 group (=-0.162, <0.001),ALA-PDT2 group (=-0.254, <0.001),and ALA-PDT3 group (=-0.352, <0.001).The values of the XTT assay were were 0.462±0.028,0.465±0.044,0.437±0.047,0.301±0.040,0.207±0.001,and 0.110±0.007,respectively,in ALA-PDT-group,ALA group,LED group,ALA-PDT1 group,ALA-PDT2 group,and ALA-PDT3 group.Although the values of XTT assay in ALA(=-0.044, =1.000)and LED groups (=-0.020, =1.000)did not significantly differ from that in ALA-PDT-group,it was significantly higher in ALA-PDT-group than in ALA-PDT1 group (=1.175, <0.001),ALA-PDT2 group (=1.942, <0.001),and ALA-PDT3 group (=-0.352, =2.742, <0.001). ALA-PDT has an inhibitory effect on biofilm.ALA-PDT destroys biofilm structure and inhibits biofilm viability.
Aminolevulinic Acid ; Biofilms ; Photochemotherapy ; Photosensitizing Agents ; Propionibacterium acnes

BACKGROUND: Acne is principally a disorder of adolescence. However, a number of observational studies have documented a significant degree of acne in adult women. One study found a difference in women between late-onset acne and acne that persisted from adolescence. There were significant higher sebum excretion rates among women whose acne originated during the teenage years compared with late-onset acne groups. OBJECTIVE: The purpose of this study was to examine the clinical features of patients with acne and to compare the sebum excretion rates and the density of P acnes in adult acne with that in adolescent acne. METHODS: Thirty nine patients with acne vulgaris were clinically evaluated. Sebum secretion rates were evaluated by Sebutape method. The density of P acnes counted by scrub method. RESULTS: 1. The severity grades were mild to moderate in adult acne groups, consisting with the lower acne lesion counts than that of adolescent acne groups. 2. Sebum secretion rates by Sebutape(R) method showed different patterns in two groups. The mean value in the adult acne groups was lower than that in adolescent acne groups, but not statistically significant. Chin area dominant pattern, shown in adult acne groups, were not apparent in adolescent acne groups. 3. The density of P acnes was a lower mean value in the adult acne groups, but not statistically significant. Only in adolescent acne groups, the severity grades are well correlated to the density of P acnes. CONCLUSION: Adult acne was mild to moderate in severity. Clinically, adult acne differs from adolescent acne in that the lesions are located most commonly around the chin. Sebum excretion rate was the highest in the chin area of patients with adult acne. But there was no significant difference in two groups. Also the density of P acnes was not significantly different in two groups.
Acne Vulgaris* ; Adolescent* ; Adult* ; Chin ; Female ; Humans ; Propionibacterium acnes* ; Propionibacterium* ; Sebum*

Chronic endophthalmitis following cataract extraction and intraocluar lens implantation has been reported occasionally and Propionibacterium acnes is Known as the most common organism. This infection is often misdiagnosed as uveitis in early stage, and diagnosis depends on anaerobic culture of intraocular specimens. Intravitreal infection of antibiotics, pars plana vitredtomy, removal of IOL with lens capsule are suggested for successful treatment of chronic endophthalmitis. We report a case of Propionibacterium acnes endophthalmitis after extracapsular cataract extraction and posterior chamber lens(PCL) implantation that was treated successfully by PCL removal and intraviteal antibiotic infection.
Anti-Bacterial Agents ; Cataract Extraction* ; Cataract* ; Diagnosis ; Endophthalmitis* ; Propionibacterium acnes* ; Propionibacterium* ; Uveitis