OBJECTIVETo detect the histological changes and the expression of proliferating cell nuclear antigen (PCNA) in junctional epithelialization of dental implants.

METHODSThe junctional epithelium was detected by HE staining and the PCNA expression was observed by immunohistochemical staining on the st, 3rd, 6th, 9th, 12th, 15th, 20th and 30th day after implantation in Beagle dogs.

RESULTSThe junctional epithelium began to form and PCNA was expressed positively 12 days after implantation. The epithelial hyperplasia tended to stabilization and the expression of PCNA weakened drastically 15 days after implantation. The epithelial attachment showed to be similar to that of natural teeth 20 days later.

CONCLUSIONThe gingiva epithelium can attach to titanium implants forming junctional epithelium which is similar to the natural teeth.

Animals ; Dental Implantation ; Dental Implants ; Dogs ; Epithelial Attachment ; Female ; Male ; Proliferating Cell Nuclear Antigen ; biosynthesis ; Titanium

OBJECTIVETo study relation of the expression of hTERT mRNA and cyclin A, p53 protein, proliferation cell nuclear antigen (PCNA) in ameloblastoma (AB) and to investigate clinical biological characteristics of AB.

METHODSThe hTERT mRNA was detected by in situ hybridization, cyclin A, p53 protein and PCNA by SP method. Normal oral mucosa and odontogenic keratocyst (OKC) are comparition.

RESULTSThe positive ratio of hTERT mRNA was 1/7 cases in normal oral mucosa. The expression of cyclin A, p53 and PCNA in normal oral mucosa were similar, and the positive cells distributed in stratum basale. In OKC, the positive cells distributed in stratum basale and super-strrtum basale. And the positive ratio of hTERT, cyclin A, p53, PCNA in OKC was 14/16 cases, 4/32 cases, 11/25 cases, 5/9 cases, respectively. In AB, the positive ratio of hTERT mRNA, cyclin A, p53 protein and PCNA was 94.4% (51/54), 66.7% (40/60), 85.7% (42/49) and 78.1% (25/32), respectively. A strong correlation was found between hTERT mRNA with cyclin A, p53 protein and PCNA (r(s) = 0.914, 0.848, 0.804, P < 0.05).

CONCLUSIONSThe expression of hTETR mRNA, cyclin A, p53 and PCNA is different in different tissues and lesions, and correlates with cell differentiation and clinical biology behaviour. Telomerase activity is related to cumulation of p53 protein, related to cell proliferation and differentiation, regulated by cyclin A, and higher in S phase.

Ameloblastoma ; metabolism ; pathology ; Cyclin A ; biosynthesis ; Humans ; Jaw Neoplasms ; metabolism ; pathology ; Proliferating Cell Nuclear Antigen ; biosynthesis ; RNA, Messenger ; biosynthesis ; Telomerase ; biosynthesis ; genetics ; Tumor Suppressor Protein p53 ; biosynthesis

Cell Line ; Humans ; Liver ; metabolism ; Membrane Potential, Mitochondrial ; Mitochondria, Liver ; metabolism ; Proliferating Cell Nuclear Antigen ; biosynthesis ; Proteins ; pharmacology

OBJECTIVETo investigate the characteristics of PCNA expression in hypertrophic scars and chronic ulcers and to discuss its relation to their formation.

METHODSThe expressive quantity and sites of PCNA were detected with the immunohistochemical SP method.

RESULTSPCNA was expressed in all samples. The expressive quantity in hypertrophic scars was higher than chronic ulcers(P < 0.01). The expressive sites of all samples were in the nucleus of fibroblasts and capillary endothelial cells.

CONCLUSIONSThe expressive quantity of PCNA was more in hypertrophic scars and less n chronic ulcers. The quantitative difference of expression between hypertrophic scars and chronic ulcers may be correlated to their formation.

Adult ; Cicatrix, Hypertrophic ; metabolism ; pathology ; Female ; Humans ; Male ; Proliferating Cell Nuclear Antigen ; biosynthesis ; Skin Ulcer ; metabolism ; pathology

Objective: To investigate the regulatory effects of bio-intensity electric field on the transformation of human skin fibroblasts (HSFs). Methods: The experimental research methods were used. HSFs were collected and divided into 200 mV/mm electric field group treated with 200 mV/mm electric field for 6 h and simulated electric field group placed in the electric field device without electricity for 6 h. Changes in morphology and arrangement of cells were observed in the living cell workstation; the number of cells at 0 and 6 h of treatment was recorded, and the rate of change in cell number was calculated; the direction of cell movement, movement velocity, and trajectory velocity within 3 h were observed and calculated (the number of samples was 34 in the simulated electric field group and 30 in 200 mV/mm electric field group in the aforementioned experiments); the protein expression of α-smooth muscle actin (α-SMA) in cells after 3 h of treatment was detected by immunofluorescence method (the number of sample was 3). HSFs were collected and divided into simulated electric field group placed in the electric field device without electricity for 3 h, and 100 mV/mm electric field group, 200 mV/mm electric field group, and 400 mV/mm electric field group which were treated with electric fields of corresponding intensities for 3 h. Besides, HSFs were divided into simulated electric field group placed in the electric field device without electricity for 6 h, and electric field treatment 1 h group, electric field treatment 3 h group, and electric field treatment 6 h group treated with 200 mV/mm electric field for corresponding time. The protein expressions of α-SMA and proliferating cell nuclear antigen (PCNA) were detected by Western blotting (the number of sample was 3). Data were statistically analyzed with Mann-Whitney U test, one-way analysis of variance, independent sample t test, and least significant difference test. Results: After 6 h of treatment, compared with that in simulated electric field group, the cells in 200 mV/mm electric field group were elongated in shape and locally adhered; the cells in simulated electric field group were randomly arranged, while the cells in 200 mV/mm electric field group were arranged in a regular longitudinal direction; the change rates in the number of cells in the two groups were similar (P>0.05). Within 3 h of treatment, the cells in 200 mV/mm electric field group had an obvious tendency to move toward the positive electrode, and the cells in simulated electric field group moved around the origin; compared with those in simulated electric field group, the movement velocity and trajectory velocity of the cells in 200 mV/mm electric field group were increased significantly (with Z values of -5.33 and -5.41, respectively, P<0.01), and the directionality was significantly enhanced (Z=-4.39, P<0.01). After 3 h of treatment, the protein expression of α-SMA of cells in 200 mV/mm electric field group was significantly higher than that in simulated electric field group (t=-9.81, P<0.01). After 3 h of treatment, the protein expressions of α-SMA of cells in 100 mV/mm electric field group, 200 mV/mm electric field group, and 400 mV/mm electric field group were 1.195±0.057, 1.606±0.041, and 1.616±0.039, respectively, which were significantly more than 0.649±0.028 in simulated electric field group (P<0.01). Compared with that in 100 mV/mm electric field group, the protein expressions of α-SMA of cells in 200 mV/mm electric field group and 400 mV/mm electric field group were significantly increased (P<0.01). The protein expressions of α-SMA of cells in electric field treatment 1 h group, electric field treatment 3 h group, and electric field treatment 6 h group were 0.730±0.032, 1.561±0.031, and 1.553±0.045, respectively, significantly more than 0.464±0.020 in simulated electric field group (P<0.01). Compared with that in electric field treatment 1 h group, the protein expressions of α-SMA in electric field treatment 3 h group and electric field treatment 6 h group were significantly increased (P<0.01). After 3 h of treatment, compared with that in simulated electric field group, the protein expressions of PCNA of cells in 100 mV/mm electric field group, 200 mV/mm electric field group, and 400 mV/mm electric field group were significantly decreased (P<0.05 or P<0.01); compared with that in 100 mV/mm electric field group, the protein expressions of PCNA of cells in 200 mV/mm electric field group and 400 mV/mm electric field group were significantly decreased (P<0.05 or P<0.01); compared with that in 200 mV/mm electric field group, the protein expression of PCNA of cells in 400 mV/mm electric field group was significantly decreased (P<0.01). Compared with that in simulated electric field group, the protein expressions of PCNA of cells in electric field treatment 1 h group, electric field treatment 3 h group, and electric field treatment 6 h group were significantly decreased (P<0.01); compared with that in electric field treatment 1 h group, the protein expressions of PCNA of cells in electric field treatment 3 h group and electric field treatment 6 h group were significantly decreased (P<0.05 or P<0.01); compared with that in electric field treatment 3 h group, the protein expression of PCNA of cells in electric field treatment 6 h group was significantly decreased (P<0.01). Conclusions: The bio-intensity electric field can induce the migration of HSFs and promote the transformation of fibroblasts to myofibroblasts, and the transformation displays certain dependence on the time and intensity of electric field.
Actins/biosynthesis* ; Cell Differentiation/physiology* ; Cell Movement/physiology* ; Electric Stimulation Therapy ; Electricity ; Fibroblasts/physiology* ; Humans ; Myofibroblasts/physiology* ; Proliferating Cell Nuclear Antigen/biosynthesis* ; Skin/cytology*

OBJECTIVETo study the expression of PCNA/bcl-2 protein in gray and normal gingival tissue, and to investigate the biological effect of procelain-fused-to-metals (PFM).

METHODSGray gingival tissue animal model was established by PFM prosthesis and immunohistochemistry S-P method was used to detect the PCNA/bcl-2 protein expression in gingival tissue after 3 months, 6 months in PFM groups and control group.

RESULTSThe expression of PCNA had significant difference between the 3th month group and the control group (P < 0.05); no significant difference was found between the 6th month group and the control group (P > 0.05). The expression of bcl-2 had no significant difference between the 6th month group and the control group (P > 0.05).

CONCLUSIONThere were a correlation between the expression of PCNA and inflammation of the gray gingival tissue; The expression of PCNA and bcl-2 protein does not indicate that gray gingival tissue had dysplasia change.

Animals ; Crowns ; Gingiva ; metabolism ; pathology ; Metal Ceramic Alloys ; Proliferating Cell Nuclear Antigen ; biosynthesis ; Proto-Oncogene Proteins c-bcl-2 ; biosynthesis ; Rabbits

To investigate the role and mechanism of mechanical stress on arterial remodeling, a new model of common carotid artery exposed to stress in vivo was established in rat, in which the change of pressure is the only influencing factor. The effect of high pressure on the morphology and expression of alpha-actin and proliferating cell nuclear antigen (PCNA) of vascular smooth muscle cells (VSMCs) in the artery was assessed. The arteries were perfused by both high pressure (160 mmHg) and normal pressure (80 mmHg) for 6 hours. The changes of morphology, expression of alpha-actin and PCNA in VSMCs were studied by histology and immunohistochemistry. The results showed that the new model could be controlled well in pressure and frequency. The euchromatin was increased and PCNA-positive particles were observed in the nuclei of VSMCs, but the expression of alpha-actin was decreased when the arteries were exposed to the high pressure. The new model has been successfully established, which provides a new tool for studying the effect of mechanical stress on arterial remodeling. In this experiment, VSMCs underwent a transformation from contractile phenotype into synthetic phenotype and tended to proliferate in response to the high pressure.
Actins ; biosynthesis ; Animals ; Carotid Artery, Common ; cytology ; physiology ; Chemotherapy, Cancer, Regional Perfusion ; Male ; Muscle, Smooth, Vascular ; cytology ; Pressure ; Proliferating Cell Nuclear Antigen ; biosynthesis ; Rats ; Rats, Sprague-Dawley

OBJECTIVETo investigate the effect of butyrylchitosan on the expression of proliferating cell nuclear antigen ( PCNA) in fibroblast proliferation of rabbit eyes after filtering operation.

METHODSTwenty-four New Zealand rabbits were randomly divided into 2 groups, with 12 rabbits in each group. Rabbits in one group received butyrylchitosan under scleral patch of trabeculectomy in right eyes and trabeculectomy in left eyes (trabeculectomy group). Rabbits in the other group received mitomycin C (MMC) in trabeculectomy in right eyes (MMC group) and without operation in left eyes. Rabbits were killed 1, 4, and 12 weeks after operations. Immunohistochemical staining was used to detected PCNA expression in fibroblast.

RESULTSAfter use of butyrylchitosan, the PCNA expression significantly decreased compared with trabeculectomy group (P < 0. 001). PCNA expression in MMC group was significantly lower than in trabeculectomy group (P <0. 001).

CONCLUSIONUsing butyrylchitosan under scleral patch of trabeculectomy decreases PCNA expression in proliferating cell and inhibits the scarring at filtering site.

Animals ; Cell Proliferation ; drug effects ; Chitosan ; analogs & derivatives ; pharmacology ; Female ; Fibroblasts ; cytology ; metabolism ; Filtering Surgery ; Male ; Membranes, Artificial ; Proliferating Cell Nuclear Antigen ; biosynthesis ; Rabbits

Dimethyl sulfoxide (DMSO), a well-known differentiation inducer in several myeloid cells, induces G1 phase arrest in many cell lines. In this study, we investigated the possibility of using DMSO to arrest H18 hybridoma cells to the G1 phase and monitor whether the arrest improves antibody production. We showed that DMSO in concentration ranging between 0.3% and 0.6% efficiently arrested H18 hybridoma cells in G1 phase. In our experiment, > 80% of cells grown for 36h in presence of the 0.6% DMSO were arrested in G1. Furthermore, expression levels of P27 were up-regulated tow fold during the G1 phase. Higher concentration of DMSO at 0.9% leads to cytotoxicity. Herein we show a simple way, a two-stage process for antibody production, which consists of a proliferation phase leading to the desired cell density, followed by an extended production phase during which the cells remain at G1 phase. Our observation that the addition of DMSO results in increase antibody production is of significance in further use of hybridoma cells in high density large scale cell culture.
Animals ; Antibodies, Monoclonal ; biosynthesis ; Cell Proliferation ; drug effects ; Dimethyl Sulfoxide ; pharmacology ; G1 Phase ; drug effects ; Hybridomas ; drug effects ; immunology ; Mice ; Proliferating Cell Nuclear Antigen ; analysis

OBJECTIVEThis study was to determine the proliferating and biosynthetic characters of fibroblasts from abnormal scars and normal skins after complete contact in vitro.

METHODS6 samples of keloid, hypertrophic scar and normal skin were collected respectively. Proliferation, inhibition and biosyntheses of different fibroblasts were investigated by detecting proliferating cell nuclear antigen(PCNA), P16, type I and III collagen proteins, and Pro alpha 1 (I) and Pro alpha 1 (III) procollagen gene expressions under the condition of fibroblast complete contact by means of cell culture, immunohistochemistry and molecular biological techniques, etc.

RESULTSOverlap and high levels of proliferating activity and biosyntheses of complete contact fibroblasts from keloids indicated that these fibroblasts lost the behavior of contact inhibition and density inhibition. However, proliferating activity and biosynthetic function of complete contact fibroblasts from normal skins reduced dramatically. Biosynthetic function of complete contact fibroblasts from hypertrophic scars was still very strong, but proliferating activity was lower than keloid and higher than normal skin.

CONCLUSIONProliferating and biosynthetic characters of complete contact fibroblasts from abnormal scars and normal skins were different, which may play an important role in the mechanisms of abnormal scar formation.

Adult ; Cell Division ; Cicatrix, Hypertrophic ; metabolism ; pathology ; Collagen ; biosynthesis ; Female ; Fibroblasts ; physiology ; Humans ; Immunohistochemistry ; In Situ Hybridization ; Male ; Middle Aged ; Proliferating Cell Nuclear Antigen ; analysis