1.A Research on Dactylogram of She Nationality Juveniles
Changjie LIU ; Yaoyun GUO ; Hua CHEN ; Peixun WU ; Tiandian WENG
Progress of Anatomical Sciences 2001;7(1):36-38
Objective To find out the distribution of the normal rating dactylogram of the juveniles of the She Nationality. Methods According to living body measure need, Prussian blue was used. Result Each type of dactylogram in number was as follows: LU>WS>WD>AT>AS. Ridgs count of each finger in quantitative order:thumb>ring finger>middle finger>index finger>little finger. Conclusion The rate of appearance of normal finger prints in various nationalities and regions has obvious difference, the normal rating of the dactylogram of the juveniles of the She Nationality differs from that of the local juveniles of the Han nationality.
2.Observation on ER Ultrastructures in Several Human Embryonic Cells with Different Gestations
Yidi WU ; Jindan SONG ; Yunqing WANG
Progress of Anatomical Sciences 2001;7(1):33-35
Objective To observe the ER ultrastructures in humn embryonic epithelial cells of colonic mucosa、renal tubule and hepatocytes and also their alterations in embryogenesis.Methods Transmission electromicroscopy technique.Results With the embryonic development, the ER increased in amount, became complex in structure and with its characteristic ER structures in different cells. Conclusion The changes of ER structures are one of the characters during cell differentiation.
3.Ultrastructural Changes of Human Fetal Myocytes in Different Months
Suxia SHAO ; Lei ZHANG ; Xianglin DUAN ; Ping LU ; Jibiao LI
Progress of Anatomical Sciences 2001;7(1):30-32
Objective To study the ultrastructural changes of 8 human fetus hearts of different months Method by TEM and SEM chemical digestion. Results The shape and ultrastructure of myocytes became complicated gradually with development. Conclusion All the changes are related to function of blood supply of human fetal heart.
4.Immunohistochemical Study of Insulin-Like Growth Factor-I in Human Fetal Organs
Fan WANG ; Huijun YANG ; Ruixiang LI ; Aidong LI
Progress of Anatomical Sciences 2001;7(1):27-29
Objective To study the distribution of insulin-like Growth Factors (IGFs) in liver、 spleen、kidney、thymus and supraadrenal glands of fetus from 16 to 24 weeks.Method Using ABC immunohistochemistry. Results IGF-I positive cells were found in these organs during this period of fetal life, but the differences of number and reaction intensity of IGF-I positive cells appeared in different organs and individuals.Conclusion These organs tissues can express IGF-I during fetal development and play paracrine and/or autocrine role in cellular proliferation and differentiation of these fetal tissue.
5.Morphometric Analysis of Kidney Development in Mouse
Min GUO ; Jing DU ; Lijing YAN ; Youzhi SHAO
Progress of Anatomical Sciences 2001;7(1):25-26
Objective To analyse the development of mouse kidney. Method Sterological methods were used in this study.Results The nephrogenic zone appeared in 14th day's kidney of the fetus development, medulla could be found in the late stage of fetus development kidney and developed after birth. Inner medulla were observed on 21st day after birth, nephrogenic zone disappeared on 7th day after birth. Morphometric analysis proves that medulla developed mainly after birth, cortex volume also developed rapidly after birth, especially after 21st day postnatally, the development of corpuscle number was finished before 7th day postnatally. Conclusion The development of mouse kidney begins on 14th day of embryo and stops on 21st day after birth, the period of the medulla development is between E 18 days and 21 days after birth.
6.Effects of Estradiol-17β on the Synthesis of Gonadotropin Releasing Hormone and Growth Hormone in Submandibular Gland of Rat
Xiaoning HAN ; Jiafei YAO ; Guangfeng LIU ; Wei HE
Progress of Anatomical Sciences 2001;7(1):19-21
Objective To study the effects of estradiol-17β (17β-E2) on the synthesis of GnRH and GH in submandibular gland of rat. Method Immunohistochemical ABC method was used to observe the localization of GnRH and GH in submandibular gland of rat.Results The synthsis of GnRH was promoted and the synthesis of GH was inhibited by 17β-E2 in rat's submandibular gland. Conclusion The 17β-E2 may play an important regulative role in the synthesis of GnRH and GH.
7.Observation of Microvasculature in Different Parts of Cerebellum Under the Optical Microscopy
Progress of Anatomical Sciences 2001;7(1):16-18
Objective The microvasculature of the anterior lobe, posterior lobe and cerebellar vermis was observed under the optical microscope in 6 rats. Methods The tannic acid-ferric chloride method (TAFM) was used to stain the vessels in cerebellum. Results The arteries within the cortex and medulla of the cerebellum originated from pia mater arteries and central branches of the cerebellar arteries, the branches of pia mater arteries mostly penetrated into the cerebellar cortex vertically and formed capillary net in the molecular layer, purkinje layer and granular layer, the arteriole in the medulla of cerebellar posterior lobe branched and formed clawed-like figurations. These vessels beneath lobule cortex connected mutually by vessel anatomosis across the medulla. Conclusion The arteriole in the cerebellum can be manifested distinctly in a strong three-dimension by TAFM and the vessel anatomosis in deep layer of cerebellum can be easily observed.
8.Immunohistochemical Studying on Subventricular Zone in Lateral Wall of Lateral Ventricle of Adult Rat
Deguang WANG ; Fengzhen ZHANG ; Youting CHEN ; Meishen WANG
Progress of Anatomical Sciences 2001;7(1):13-15
Objective The cells of subventricular zone in lateral wall of lateral ventricle of adult rats were localized. Method Immunohistochemistry. Results Polysialylated neuronal cell adhesion molecule and phosphotyrosine immunoreactive cells were found in subventricular zone, and they have same distributions, however, no any parvalbulin immunoreactive cells were found in subventricular zone. Conclusion neuronal precursor cells in subventricular and expression of phosphotyrosine are associated with proliferation of neuronal precursor cells in subventricular zone.
9.The Projection from the Long Descending Propriospinal Tract to the Lumbar Enlargement in the Rabbit
Yuping MA ; Xiulai GAO ; Yan WU ; Qunyuan XU ; Yaliang CHEN
Progress of Anatomical Sciences 2001;7(1):10-12
ObjectiveTo explore the projections of the long descending propriospinal tracts to lumbar enlargement. Method Anterograde tracing with cupric-silver staining. 10 animals were injected b biotinylated dextran in the upper cervical cord. Following survival times of 14 days, projections of the long descending propriospinal tracts were immunohistochemically demonstrated in the lumbar enlargement.Results The degenerated terminals and labeled terminals were found in the bilateral gray matter of the lumbar enlargement, but predominantly ipsilaterally. The terminals were widely distributed in laminal Ⅴ-Ⅸ, heavily Ⅶ and Ⅷ. Conclusion The long descending propriospinal tract from the upper cervical cord projects to the lumbar enlargement.
10.Experimental Study of Effect of Aluminum on Learning and Memory and the Changes of Synaptic Interface Constructure in Hippocampus CA3 Area in Rats
Yuhong JING ; Shenyuan FENG ; Fei WANG
Progress of Anatomical Sciences 2001;7(1):4-6
Objective To study the effect of aluminum on learning and memory and the synaptic interface constructure in hippocampus CA3 area in rats. Methods The electrical maze were used to test the behavioral reaction of rats. Some parameters of synaptic interface structure were measured by the method of ultrastructural quantitative analysis in hippocampus CA3 area in the rats. Results The time coming through the E-maze prolonged siginificantly in the experimental group compared with the normal control (P<0.01), meanwhile the cyto-apparatus demonstrated the pathogenetic changes. Amounts of synapse obviously reduced and the post-synaptic density turned thinner in experimental group than in the control group (P<0.01). Conclusion Aluminum can cause the malfunction on learning and memory, which related to the plastic of synapse.