1.Research progress of tannins in traditional Chinese medicines in recent ten years.
Xuan-Xuan ZHU ; Lu BAI ; Xiao-Qian LIU ; Yao-Hua LIANG ; Li-Mei LIN ; Wei-Hong FENG ; Zhi-Min WANG ; Chun LI ; Duan-Fang LIAO
China Journal of Chinese Materia Medica 2021;46(24):6353-6365
In this paper, the newly isolated tannins were sorted after a review of the literature concerning tannins in recent 10 years, and their research progress was summarized in terms of extraction, isolation, pharmacological activity and metabolism. Hydrolysable tannins and condensed tannins are the main structural types. Modern research shows that tannins have many pharmacological effects, such as bacteriostasis, antioxidation, antitumor, antivirus and blood glucose reduction, and have broad development prospects. They are usually extracted by water, ethanol and acetone and isolated and purified by macroporous resin and gel column chromatography. The packings commonly adopted for the column chromatography mainly included Sephadex LH-20, Diaion HP-20, MCI-gel CHP-20 and Toyopearl HW-40. Modern analytical techniques such as nuclear magnetic resonance spectroscopy(NMR), fast atom bombardment mass spectrometry(FAB-MS) and circular dichroism(CD) are generally used for the structural identification of tannins. Howe-ver, their isolation, purification and structural identification are still challenging. It is necessary to use a variety of high-throughput screening methods to explore their pharmacological activities and to explore the material basis responsible for their functions through experiments in vivo.
China
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Hydrolyzable Tannins
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Medicine, Chinese Traditional
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Proanthocyanidins
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Tannins
2.Ameliorative effects of type-A procyanidins polyphenols from cinnamon bark in compound 48/80-induced mast cell degranulation.
Amit D KANDHARE ; Urmila M ASWAR ; Vishwaraman MOHAN ; Prasad A THAKURDESAI
Anatomy & Cell Biology 2017;50(4):275-283
Allergic diseases are a significant health concern in developing countries. Type-A procyanidin polyphenols from cinnamon (Cinnamomum zeylanicum Blume) bark (TAPP-CZ) possesses antiasthmatic and antiallergic potential. The present study was aimed at the possible anti-allergic mechanism of TAPP-CZ against the compound 48/80 (C48/80)–induced mast cell degranulation in isolated rat peritoneal mast cells (RPMCs). TAPP-CZ (1, 3, 10, and 30 µg/ml) was incubated for 3 hours with isolated, purified RPMCs. The C48/80 (1 µg/ml) was used to induce mast cell degranulation. The mast cell viability was assessed using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay whereas histamine, β-hexosaminidase (β-HEX), and interleukin-4 (IL-4) levels were determined in RPMCs. TAPP-CZ (3, 10, and 30 µg/ml) showed significant and dose-dependent decrease in a number of degranulated cells and levels of markers (histamine, β-HEX, and IL-4) as compared with C48/80 control. In conclusion, TAPP-CZ stabilizes mast cell and cause inhibition of the allergic markers such as histamine, IL-4, and β-HEX in IgE-mediated manner. The present study supports mast cell stabilization as a possible mechanism of action of TAPP-CZ against immune respiratory disorders such as asthma and allergic rhinitis.
Animals
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Asthma
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Cinnamomum zeylanicum*
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Developing Countries
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Histamine
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Interleukin-4
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Mast Cells*
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Polyphenols*
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Proanthocyanidins*
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Rats
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Rhinitis, Allergic
3.Antioxidant activity and analysis of proanthocyanidins from pine (Pinus densiflora) needles.
Yong Soo PARK ; Min Hee JEON ; Hyun Jung HWANG ; Mi Ra PARK ; Sang Hyeon LEE ; Sung Gu KIM ; Mihyang KIM
Nutrition Research and Practice 2011;5(4):281-287
In this study, we evaluated the antioxidant activity of pine needle extracts prepared with hot water, ethanol, hexane, hot water-hexane (HWH), and hot water-ethanol (HWE), using the DPPH (1,1-diphenyl-2-picrylhydrazyl) radical method. The hot water extract possessed superior antioxidant activity than the other extracts. We also compared the antioxidant activity of pine needle extracts through ROS inhibition activity in a cellular system using MC3T3 E-1 cells. The hot water extract exhibited the lowest ROS production. The pattern of HPLC analysis of each extract indicated that the hot water extract contained the highest proanthocyanidin level. The pine needle hot-water extract was then isolated and fractionated with Sephadex LH-20 column chromatography to determine the major contributor to its antioxidant activity. The No.7 and 12 fractions had high antioxidant activities, that is, the highest contents of proanthocyanidins and catechins, respectively. These results indicate that the antioxidant activity of procyanidins from the hot water extract of pine needles is positively related to not only polymeric proanthocyanidins but also to monomeric catechins. Moreover, the antioxidant activity of the pine needle hot water extract was similar to well-known antioxidants, such as vitamin C. This suggests that pine needle proanthocyanidins and catechins might be of interest for use as alternative antioxidants.
Antioxidants
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Ascorbic Acid
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Catechin
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Chromatography
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Chromatography, High Pressure Liquid
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Dextrans
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Ethanol
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Needles
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Polymers
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Proanthocyanidins
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Water
4.Effect of Plant Extracts Contained Dressing Material Which Is Rich in Procyanidins for Treatment of Deep Second Degree Burn.
Young Joo LEE ; Jaechul YOON ; Hyeong Tae YANG ; Yong Suk CHO ; Dohern KYM ; Jong Hyun KIM ; Jun HUR ; Wook CHUN ; Haejun YIM
Journal of Korean Burn Society 2014;17(1):25-29
PURPOSE: This study was planned to evaluate the effect of plant extracts contained dressing material which is rich in procyanidins for treatment of deep second degree burn. METHODS: This study conducted from September 1, 2013 to February 28, 2014. Patients with the deep dermal burn wound which is larger than 200 cm2 were enrolled in this study. The test material was applied total 3 times every 2 days when the wounds were relatively clean, with thin eschar and scab removed and the dermal layer exposed. The test and control sites were treated with hydro-foam equally. The effectiveness was evaluated by comparing the re-epithelialization rates between the test and control sites. Two burn surgeons blindly evaluated for re-epithelialization. Evaluation of adverse reaction was also performed during study period. RESULTS: Total of 40 patients were enrolled. Mean total body surface area burned% was 12.3+/-5.5% and the test material was first applied at mean post burn day # 7.2+/-2.0.The re-epithelialization period was 9.0+/-1.7 days in the test site and 11.1+/-2.0 days in the control site. In the test site, re-epithelialization was 2.1+/-1.0 days faster than in the control site (P<0.0001). There was no significant adverse reaction during study period. CONCLUSION: The plant extracts contained dressing material which is rich in procyanidins accelerates wound healing time and shows the safety.
Bandages*
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Body Surface Area
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Burns*
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Humans
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Plant Extracts*
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Proanthocyanidins*
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Re-Epithelialization
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Wound Healing
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Wounds and Injuries
5.Comparison of methods for proanthocyanidin extraction from pine (Pinus densiflora) needles and biological activities of the extracts.
Nam Young KIM ; Min Kyung JANG ; Dong Geun LEE ; Ki Hwan YU ; HyeJi JANG ; Mihyang KIM ; Sung Gu KIM ; Byung Hong YOO ; Sang Hyeon LEE
Nutrition Research and Practice 2010;4(1):16-22
Flavonoids are known to be effective scavengers of free radicals. In particular, proanthocyanidins are flavonoids that possess cardiovascular protection, antioxidative activities, and immunomodulatory activities. Here, we evaluated proanthocyanidin contents in the total polyphenolic compounds of pine needle extracts prepared by hot water, ethanol, hexane, hot water-hexane (HWH), and hot water-ethanol (HWE). Analysis of each extract indicated that the ethanol extract contained the highest proanthocyanidin concentration. The HWH and hexane extracts also contained relatively high concentrations of proanthocyanidin. On the other hand, proanthocyanidin content analyses out of the total polyphenolic compounds indicated that the HWH extract contained the highest content. These results suggest that HWH extraction is a suitable method to obtain an extract with a high level of pure proanthocyanidins and a relatively high yield. The HWH extract possessed superior activity in diverse antioxidative analyses such as 2,2-diphenyl-1-picrylhydrazyl (DPPH), ferrous ion chelating (FIC), and ferric-ion reducing power (FRAP) assays. In addition, upon assessing the effects of the pine needle extracts on macrophages (Raw 264.7 cell), the HWH extract exhibited the highest activity. In this study, we discerned an efficient extraction method to achieve relatively pure proanthocyanidins from pine needles and evaluated the biological functions of the resulting extract, which could potentially be used for its efficacious components in functional food products.
Biphenyl Compounds
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Ethanol
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Flavonoids
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Free Radicals
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Functional Food
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Hand
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Macrophages
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Needles
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Picrates
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Proanthocyanidins
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Water
6.Progress on study of pharmacological effects of procyanidins.
China Journal of Chinese Materia Medica 2009;34(6):651-655
Procyanidins is the generic term of a large class of polyphenol compounds widespread in the plant kingdom. Because of its potential ability to eliminate free radicals, and also the characteristics of high efficiency and low toxicity, it is the latest craze all over the word, widely used in medicines, cosmetics, health products, etc. This paper reviews the newest research progress of pharmacological effects of procyanidins and its possible action mechanism, such as antioxidation, cardiovascular protection, prevention and treatment of cancer, anti-inflammatory action, anti-diabetic effect, liver protection, and the role of anti-gastrelcosis, anti-radiation, improving learning and memory, promoting hair growth and so on at home and abroad in recent few years.
Animals
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Drugs, Chinese Herbal
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pharmacology
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therapeutic use
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Humans
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Proanthocyanidins
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pharmacology
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therapeutic use
8.Comparison of in vitro release behavior of proanthocyanidins flexible nanoliposomes and general nanoliposomes.
Yanjun CHEN ; Huibin WU ; Junlin YUAN ; Lei SHI ; Rixian JIN ; Huafeng LIU ; Weizheng XIONG ; Luqi HUANG
China Journal of Chinese Materia Medica 2010;35(2):169-172
OBJECTIVETo prepare flexible proanthocyanidins nanoliposomes, and explore the in vitro release behavior of proanthocyanidins flexible nanoliposomes and general nanoliposomes.
METHODFlexible proanthoeyanidins nanoliposomes were prepared proanthocyanidins using a film dispersion method, characterized by transmission electron microscope, and the in vitro release action was studied in different dissolution mediums using dynamic dialyse method with the content of total phenol as index.
RESULTThe in vitro release of both proanthocyanidins flexible nanoliposomes and general nanoliposomes were in accordance with Weibull distribution.
CONCLUSIONProanthocyanidins flexible nanoliposomes without pressure had similar in vitro release behavior with general nanoliposomes.
Drug Delivery Systems ; methods ; Liposomes ; chemistry ; ultrastructure ; Nanospheres ; chemistry ; ultrastructure ; Particle Size ; Proanthocyanidins ; chemistry
9.Purification and component identification of total proanthocyanidins in Choerospondias axillaris pericarp.
Tong JIANG ; Tong ZHU ; Fei TENG ; Dan YANG ; Jing-Jing ZHU ; Zhi-Min WANG ; Zhi-Gao LIU ; Ji-Yan LIU
China Journal of Chinese Materia Medica 2021;46(12):2923-2930
The present study determined the quantitative markers of total proanthocyanidins in the purification of the industrial waste Choerospondias axillaris pericarp based on the comparison results of high-performance liquid chromatography(HPLC) and mass spectrometry(MS) and optimized the purification process with two stable procyanidins as markers. The adsorption and desorption of five different macroporous adsorption resins, the static adsorption kinetics curve of NKA-Ⅱ resin, the maximum sample load, and the gradient elution were investigated. The UPLC-Q-TOF-MS/MS was employed for qualitative analysis of the newly-prepared total proanthocyanidins of C. axillaris pericarp. As revealed by the results, NKA-Ⅱ resin displayed strong adsorption and desorption toward total proanthocyanidins. The sample solution(50 mg·mL~(-1)) was prepared from 70% ethanol crude extract of C. axillaris pericarp dissolved in water and 7-fold BV of the sample solution was loaded, followed by static adsorption for 12 h. After 8-fold BV of distilled water and 6-fold BV of 10% ethanol were employed to remove impurities, the solution was eluted with 8-fold BV of 50% ethanol, concentrated, and dried under reduced pressure, and purified total proanthocyanidin powder was therefore obtained. Measured by vanillin-hydrochloric acid method, the purity and transfer rate of total proanthocyanidins were 47.67% and 59.92%, respectively, indicating the feasibi-lity of the optimized process. UPLC-Q-TOF-MS/MS qualitative analysis identified 16 procyanidins in C. axillaris total proanthocyanidins. The optimized purification process is simple in operation and accurate in component identification, and it can be applied to the process investigation of a class of components that are difficult to be separated and purified. It can also provide technical support and research ideas for the comprehensive utilization of industrial waste.
Adsorption
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Anacardiaceae
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Chromatography, High Pressure Liquid
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Plant Extracts
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Proanthocyanidins/analysis*
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Resins, Synthetic
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Tandem Mass Spectrometry
10.Condensed tannins from roots of Indigofera stachyodes.
Yun-Feng ZHANG ; Zhi-Xiang ZHU ; Wen-Xuan WANG ; Hang ZHANG ; Li LIU ; Shi-Lin ZHANG ; Yun-Fang ZHAO ; Yue-Lin SONG ; Jiao ZHENG ; Peng-Fei TU ; Hui-Xia HUO ; Jun LI
China Journal of Chinese Materia Medica 2021;46(16):4131-4138
Eleven condensed tannins were isolated from the roots of Indigofera stachyodes by various column chromatography techniques including silica gel, octadecyl silica(ODS), Sephadex LH-20, and semi-preparative high performance liquid chromatography(HPLC). These compounds were identified on the basis of physicochemical properties, nuclear magnetic resonance(NMR) and mass spectrometry(MS) data as stachyotannin A(1), epicatechin-(2β→O→7,4β→8)-epiafzelechin-(4β→8)-catechin(2), cinnamtannin D1(3), cinnamtannin B1(4), epicatechin-(2β→O→7,4β→8)-epiafzelechin-(4α→8)-epicatechin(5), gambiriin C(6), proanthocyanidin A1(7), proanthocyanidin A2(8), aesculitannin B(9), proanthocyanidin A4(10), and procyanidin B5(11). Compound 1 is a new compound. Compounds 2-11 were isolated from Indigofera for the first time. Furthermore, compounds 1, 2, and 4-11 showed inhibitory effects on thrombin-induced ATP release in platelets.
Chromatography, High Pressure Liquid
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Indigofera
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Magnetic Resonance Spectroscopy
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Plant Extracts
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Proanthocyanidins