1.Discriminant analysis of prion sequences for prediction of susceptibility.
Ji Hae LEE ; Se Eun BAE ; Sunghoon JUNG ; Insung AHN ; Hyeon Seok SON
Experimental & Molecular Medicine 2013;45(10):e48-
Prion diseases, including ovine scrapie, bovine spongiform encephalopathy (BSE), human kuru and Creutzfeldt-Jakob disease (CJD), originate from a conformational change of the normal cellular prion protein (PrPC) into abnormal protease-resistant prion protein (PrPSc). There is concern regarding these prion diseases because of the possibility of their zoonotic infections across species. Mutations and polymorphisms of prion sequences may influence prion-disease susceptibility through the modified expression and conformation of proteins. Rapid determination of susceptibility based on prion-sequence polymorphism information without complex structural and molecular biological analyses may be possible. Information regarding the effects of mutations and polymorphisms on prion-disease susceptibility was collected based on previous studies to classify the susceptibilities of sequences, whereas the BLOSUM62 scoring matrix and the position-specific scoring matrix were utilised to determine the distance of target sequences. The k-nearest neighbour analysis was validated with cross-validation methods. The results indicated that the number of polymorphisms did not influence prion-disease susceptibility, and three and four k-objects showed the best accuracy in identifying the susceptible group. Although sequences with negative polymorphisms showed relatively high accuracy for determination, polymorphisms may still not be an appropriate factor for estimating variation in susceptibility. Discriminant analysis of prion sequences with scoring matrices was attempted as a possible means of determining susceptibility to prion diseases. Further research is required to improve the utility of this method.
Amino Acid Sequence
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Animals
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Discriminant Analysis
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Disease Susceptibility
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Humans
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Mammals/genetics
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Mutation
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Polymorphism, Genetic
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Prion Diseases/genetics
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Prions/chemistry/genetics/*pathogenicity
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Sequence Analysis, DNA
2.Heat shock cognate 71 (HSC71) regulates cellular antiviral response by impairing formation of VISA aggregates.
Zhigang LIU ; Shu-Wen WU ; Cao-Qi LEI ; Qian ZHOU ; Shu LI ; Hong-Bing SHU ; Yan-Yi WANG
Protein & Cell 2013;4(5):373-382
In response to viral infection, RIG-I-like RNA helicases detect viral RNA and signal through the mitochondrial adapter protein VISA. VISA activation leads to rapid activation of transcription factors IRF3 and NF-κB, which collaborate to induce transcription of type I interferon (IFN) genes and cellular antiviral response. It has been demonstrated that VISA is activated by forming prion-like aggregates. However, how this process is regulated remains unknown. Here we show that overexpression of HSC71 resulted in potent inhibition of virus-triggered transcription of IFNB1 gene and cellular antiviral response. Consistently, knockdown of HSC71 had opposite effects. HSC71 interacted with VISA, and negatively regulated virus-triggered VISA aggregation. These findings suggest that HSC71 functions as a check against VISA-mediated antiviral response.
Adaptor Proteins, Signal Transducing
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biosynthesis
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chemistry
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genetics
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metabolism
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Cell Aggregation
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genetics
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GPI-Linked Proteins
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metabolism
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Gene Knockdown Techniques
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HEK293 Cells
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HSC70 Heat-Shock Proteins
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genetics
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metabolism
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Heat-Shock Response
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genetics
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Humans
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Interferon Regulatory Factor-3
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genetics
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metabolism
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Interferon-beta
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genetics
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NF-kappa B
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genetics
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Prions
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metabolism
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Receptors, Retinoic Acid
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metabolism
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Viruses
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drug effects
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metabolism
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pathogenicity