1.Clinical characteristics and outcome of invasive Prevotella infection.
Korean Journal of Medicine 2003;64(3):245-246
No abstract available.
Prevotella*
2.Isolation and Characterization of a Putative Hemin-binding Protein from Prevotella intermedia.
The Journal of the Korean Academy of Periodontology 2000;30(4):737-745
No abstract available.
Prevotella intermedia*
;
Prevotella*
3.Cytotoxic effects of prevotella nigrescens on cultured cells.
Jin Soon HAN ; Eun Sook KIM ; Su Jong LEE ; Yong Ouk YOU ; Kyung Soo HAN ; Mi Kyung IM
Journal of Korean Academy of Conservative Dentistry 2002;27(2):183-195
No abstract available.
Cells, Cultured
;
Prevotella
;
Prevotella nigrescens
4.Interleukin-8 production and interleukin-8 mRNA expression induced by lipopolysaccharides from Prevotella intermedia and Prevotella nigrescens in monocyte-derived macrophages.
The Journal of the Korean Academy of Periodontology 2009;39(2):177-184
PURPOSE: Interleukin-8 (IL-8) is an important mediator of immune and inflammatory reactions and is produced by a variety of different cell types. This study was undertaken to investigate the effects of lipopolysaccharides (LPSs) from Prevotella intermedia and Prevotella nigrescens, the major causes of inflammatory periodontal disease, on the production of IL-8 and the expression of IL-8 mRNA in differentiated THP-1 cells, a human monocytic cell line. METHODS:LPSs from P. intermedia ATCC 25611 and P. nigrescens ATCC 33563 were prepared by the standard hot phenol-water method. THP-1 cells were incubated in the medium supplemented with phorbol myristate acetate to induce differentiation into macrophage-like cells. RESULTS: We found that LPS preparations from P. intermedia and P. nigrescens can induce IL-8 mRNA expression and stimulate the release of IL-8 in differentiated THP-1 cells without additional stimuli. CONCLUSIONS: There are no previous reports of the ability of P. intermedia and P. nigrescens LPS to stimulate the release of IL-8, and the present study clearly shows, for the first time, that LPSs from P. intermedia and P. nigrescens fully induced IL-8 mRNA expression and IL-8 production in differentiated human monocytic cell line THP-1. The ability of P. intermedia and P. nigrescens LPS to promote the production of IL-8 may be important in the pathogenesis of inflammatory periodontal disease.
Cell Line
;
Humans
;
Interleukin-8
;
Lipopolysaccharides
;
Macrophages
;
Periodontal Diseases
;
Phorbols
;
Prevotella
;
Prevotella intermedia
;
Prevotella nigrescens
;
RNA, Messenger
;
Tetradecanoylphorbol Acetate
5.Isolation and Partial Characterization of a 50 kDa Hemin-regulated Cell Envelope Protein from Prevotella nigrescens.
Kyung Mi KIM ; Jeom IL CHOI ; Sung Jo KIM
The Journal of the Korean Academy of Periodontology 2002;32(2):351-360
In the study presented here, identification, purification, and partial characterization of a hemin-regulated protein in Prevotella nigrescens were carried out. The results of this study confirm that the availability of hemin influences the expression of a selected membrane protein as well as the growth rate of P. nigrescens ATCC 33563. The 50 kDa cell envelope associated protein, whose expression is hemin regulated, is considered to be a putative hemin-binding protein from P. nigrescens. Disulfide bonds were not present in this protein, and N'-terminal amino acid sequence analysis revealed that this protein belongs to a new, so far undescribed protein. The 50 kDa protein was found to be rich in hydrophilic amino acids, with glycine comprising approximately 60% of the total amino acids. The study described here is the first to identify, purify, and biochemically characterize a putative hemin-binding protein from P. nigrescens. Work is in progress to further characterize the molecular structure of this protein.
Amino Acids
;
Glycine
;
Hemin
;
Membrane Proteins
;
Molecular Structure
;
Prevotella nigrescens*
;
Prevotella*
;
Sequence Analysis, Protein
6.Study on development of DNA probe for identification of Prevotella intermedia G8-9K-3.
Jong Sung BAK ; Se Hoon KIM ; Dong Kie KIM ; Jin Hyo SEONG ; Byung Ock KIM ; Mi Kwang KIM ; Joong Ki KOOK
The Journal of the Korean Academy of Periodontology 2002;32(2):281-290
The purpose of this study is to develop species-specific DNA probe for detection and identification of Prevotella intermedia (P. intermedia) G8-9K-3. This study procedure includes (1) whole-genomic DNA extraction of P. intermedia G8-9K-3 (2) construction of the genomic DNA library, (3) screening of strain-specific DNA probe by reverse dot hybridization, (4) confirmation of strain-specific DNA probe by Southern blot hybridization, (5) determination of nucleotide sequences of strain-specific DNA probe. Twenty-eight recombinant plasmids containing Hind III-digested DNA fragments of P. intermedia G8-9K-3 were obtained. Reverse dot Hybridization and Southern blot analysis data showed that one of them, Pig3, could be P. intermedia G8-9K-3-specific DNA probe. This datum indicates that this Pig3 DNA probe could be useful in detection and identification of the P. intermedia G8-9K-3 strain.
Base Sequence
;
Blotting, Southern
;
DNA*
;
Gene Library
;
Mass Screening
;
Plasmids
;
Prevotella intermedia*
;
Prevotella*
7.Influence of the Sonic Power Toothbrush on Reduction of Gingival inflammation and on the Amount of Interleukin-6, Prevotella intermedia and Actinobacillus actinomycetemcomitans in Periodontal Pocket.
Ji Youn HONG ; Gyung Joon CHAE ; Sung Won JUNG ; Yoo Jung UM ; Seong Ho CHOI ; Chong Kwan KIM
The Journal of the Korean Academy of Periodontology 2007;37(Suppl):409-426
No abstract available.
Actinobacillus*
;
Aggregatibacter actinomycetemcomitans*
;
Inflammation*
;
Interleukin-6*
;
Periodontal Pocket*
;
Prevotella intermedia*
;
Prevotella*
8.Strain-specific PCR Primers for the Detection of Prevotella intermedia ATCC 49046.
Min Jung KIM ; Jeong Bum MIN ; Sun A LIM ; Joong Ki KOOK
International Journal of Oral Biology 2011;36(2):79-82
The aim of this study was to develop Prevotella intermedia ATCC 49046-specific PCR primers designed based on the nucleotide sequence of a DNA probe Pig28. The strain-specificity of the PCR primers, Pig28-F1/Pig28-R1, was confirmed with 9 strains of P. intermedia and 25 strains (15 species) of Prevotella species. The detection limit of the PCR primers was 2 pg of the purified genomic DNA of P. intermedia ATCC 49046. These PCR primers were found to be useful for identifying P. intermedia ATCC 49046, particularly for determining the authenticity of the strain.
Base Sequence
;
DNA
;
Limit of Detection
;
Polymerase Chain Reaction
;
Prevotella
;
Prevotella intermedia
;
Sprains and Strains
9.Chemical and Immunobiological Characterization of Lipopolysaccharides from Prevotella intermedia and Prevotella nigrescens.
The Journal of the Korean Academy of Periodontology 2004;34(2):461-474
The purpose of this study was to assess some biological activities of lipopolysaccharides (LPSs) from P. intermedia and P. nigrescens. LPS was prepared by the standard hot phenol-water method. NO production was assayed by measuring the accumulation of nitrite in culture supernatants. TNF-alpha production was determined by enzyme-linked immunosorbent assay. Western blot analysis of iNOS and analysis of reverse transcription (RT)-PCR products were carried out. LPS from P. intermedia demonstrated higher KDO content than those from two stains of P. nigrescens. LPSs from P. intermedia and P. nigrescens were mitogenic for spleen cells of BALB/C mouse. The present study clearly shows that LPSs from P. intermedia and P. nigrescens fully induced iNOS expression and NO production in RAW264.7 cells in the absence of other stimuli. Moreover, LPSs from P. intermedia and P. nigrescens clearly induced TNF-alpha production in RAW264.7 cells. The biological activities of LPS from P. intermedia was found to be comparable to those of P. nigrescens LPS. The ability of LPSs from P. intermedia and P. nigrescens to promote the production of NO and TNF-alpha may be important in the pathogenesis of inflammatory periodontal disease.
Animals
;
Blotting, Western
;
Coloring Agents
;
Enzyme-Linked Immunosorbent Assay
;
Lipopolysaccharides*
;
Mice
;
Nitric Oxide
;
Periodontal Diseases
;
Prevotella intermedia*
;
Prevotella nigrescens*
;
Prevotella*
;
Reverse Transcription
;
Spleen
;
Tumor Necrosis Factor-alpha
10.The Frequency of Detecting Prevotella intermedia and Prevotella nigrescens in Korean Adult Periodontitis Patients.
Seung Yup PECK ; Young KU ; In Cheol RHYU ; Byung Do HAHM ; Soo Boo HAN ; Sang Mook CHOI ; Chong Pyoung CHUNG
The Journal of the Korean Academy of Periodontology 2000;30(2):419-427
Prevotella intermedia has been implicated as a potent pathogen in many kinds of periodontal, pulpal and periapical diseases. However, it has been isolated from periodontally healthy adults and from edentulous children as well. The intraspecies heterogeneity of Prevotella intermedia has been demonstrated in early studies and finally Shah & Gharbia confirmed the existence of 2 DNA homology groups and proposed dividing Prevotella intermedia into 2 species, Prevotella intermedia and Prevotella nigrescens. This study was designed to examine the frequency of Prevotella intermedia and Prevotella nigrescens in diseased periodontal pockets and healthy gingival sulcus of Korean people by PCR based on 16s ribosomal DNA sequence. One hundred adults who had adult periodontitis but not taken any periodontal treatment or antibiotics during previous 6 months and 50 adults who had healthy periodontal tissue were selected for this study. The sulcular fluid was collected into VMGA by sterilized paper point and diluted to 1,000 times in anaerobic chamber. 100ml of sample was cultured in 37degrees C for 10 days. Among the bacterial colonies, BPB were selected and cultured in BHI broth and then Prevotella intermedia was identified through Gram staining and biochemical test. Identified Prevotella intermedia was cultured again and centrifuged. DNA was extracted from the pellet using several reagents. PCR was performed by previously designed primer. The results were followed. 1.BPB were isolated from 39 of 100 samples of diseased periodontal pockets(39%). 2.Prevotella intermedia was identified from 24 of 39 BPB samples. 3.Among 24 Prevotella intermedia, 21 were confirmed as Prevotella intermedia(87.5) and 2 were confirmed as Prevotella nigrescens(8.33%). 4.BPB were isolated from 9 of 50 samples of periodontally healthy patients. Among them only two were identified as Prevotella intermedia, that is, one was confirmed as Prevotella intermedia and the other was Prevotella nigrescens.
Adult*
;
Anti-Bacterial Agents
;
Child
;
Chronic Periodontitis*
;
DNA
;
DNA, Ribosomal
;
Humans
;
Indicators and Reagents
;
Periapical Diseases
;
Periodontal Pocket
;
Polymerase Chain Reaction
;
Population Characteristics
;
Prevotella intermedia*
;
Prevotella nigrescens*
;
Prevotella*