1.Polymorphic and sex-limited phosphoglucomutase in Parastrongylus cantonensis (Nematoda: Angiostrongylidae).
Eamsobhana Praphathip ; Dechkum Naowarat ; Hoi-Sen Yong
Tropical biomedicine 2005;22(2):123-9
Phosphoglucomutase was studied by polyacrylamide gel electrophoresis in the Thailand and Hawaii isolates of Parastrongylus cantonensis (also known as Angiostrongylus cantonensis). Two loci were present. The faster-moving locus (PGM-1) was polymorphic in the Hawaii isolate, represented by two alleles - the faster-moving, less common Pgm-1A and the slower-moving, more common Pgm-1B . It was monomorphic for the faster-moving allele Pgm-1A in the Thailand isolate. The slower-moving locus (PGM-2) was invariant, with a single band of enzyme activity, in the female worms of both the Thailand and Hawaii isolates. There was no detectable enzyme activity at this PGM-2 locus in the male worms of both isolates. The non-expression or 'null' PGM-2 phenotype in the male worms was presumed to be sex- limited. The present findings differ significantly in several aspects (polymorphic locus, proportion of polymorphic loci, heterozygosity, deviations from Hardy-Weinberg expectations, sex-limited expression) from the Japan isolate of P. cantonensis reported in the literature.
Gender
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7-hydroxy-5,11-dioxotetranorprostane-1,16-dioic acid
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Thailand
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Phosphoglucomutase
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Hawaii
2.Evaluation of Rapid IgG4 Test for Diagnosis of Gnathostomiasis
Yue WANG ; An MA ; Xiao-Long LIU ; Praphathip EAMSOBHANA ; Xiao-Xian GAN
The Korean Journal of Parasitology 2021;59(3):257-263
Human gnathostomiasis is a parasitic disease caused by Gnathostoma nematode infection. A rapid, reliable, and practical immunoassay, named dot immuno-gold filtration assay (DIGFA), was developed to supporting clinical diagnosis of gnathostomiasis. The practical tool detected anti-Gnathostoma-specific IgG4 in human serum using crude extract of third-stage larvae as antigen. The result of the test was shown by anti-human IgG4 monoclonal antibody conjugated colloidal gold. The sensitivity and specificity of the test were both 100% for detection in human sera from patients with gnathostomiasis (13/13) and from healthy negative controls (50/50), respectively. Cross-reactivity with heterogonous serum samples from patients with other helminthiases ranged from 0 (trichinosis, paragonimiasis, clonorchiasis, schistosomiasis, and cysticercosis) to 25.0% (sparganosis), with an average of 6.3% (7/112). Moreover, specific IgG4 antibodies diminished at 6 months after treatment. This study showed that DIGFA for the detection of specific IgG4 in human sera could be a promising tool for the diagnosis of gnathostomiasis and useful for evaluating therapeutic effects.
3.Evaluation of Rapid IgG4 Test for Diagnosis of Gnathostomiasis
Yue WANG ; An MA ; Xiao-Long LIU ; Praphathip EAMSOBHANA ; Xiao-Xian GAN
The Korean Journal of Parasitology 2021;59(3):257-263
Human gnathostomiasis is a parasitic disease caused by Gnathostoma nematode infection. A rapid, reliable, and practical immunoassay, named dot immuno-gold filtration assay (DIGFA), was developed to supporting clinical diagnosis of gnathostomiasis. The practical tool detected anti-Gnathostoma-specific IgG4 in human serum using crude extract of third-stage larvae as antigen. The result of the test was shown by anti-human IgG4 monoclonal antibody conjugated colloidal gold. The sensitivity and specificity of the test were both 100% for detection in human sera from patients with gnathostomiasis (13/13) and from healthy negative controls (50/50), respectively. Cross-reactivity with heterogonous serum samples from patients with other helminthiases ranged from 0 (trichinosis, paragonimiasis, clonorchiasis, schistosomiasis, and cysticercosis) to 25.0% (sparganosis), with an average of 6.3% (7/112). Moreover, specific IgG4 antibodies diminished at 6 months after treatment. This study showed that DIGFA for the detection of specific IgG4 in human sera could be a promising tool for the diagnosis of gnathostomiasis and useful for evaluating therapeutic effects.