3.Virulence of Cultured Supernatant in Porphyromonas gingivalis W50 under Hemin- and Menadione-Limited Culture Condition.
Kang Ju KIM ; Eun Cheol KIM ; Ki Gyung KIM ; Hyun Ok LEE ; Seon Il JANG ; Chong Pyoung CHUNG
The Journal of the Korean Academy of Periodontology 1997;27(3):515-524
No abstract available.
Porphyromonas gingivalis*
;
Porphyromonas*
;
Virulence*
4.Identification of differentially expressed proteins in the bacterial biofilm.
The Journal of the Korean Academy of Periodontology 2005;35(2):271-275
No abstract available.
Biofilms*
;
Porphyromonas gingivalis
5.T lymphocyte response to periodontal complex bacterial biofilm.
Jeom IL CHOI ; Sung Jo KIM ; Su Jin KIM
The Journal of the Korean Academy of Periodontology 2002;32(1):33-39
No abstract available.
Biofilms*
;
Lymphocytes*
;
Porphyromonas gingivalis
6.Fusobacterium nucleatum modulates serum binding to Porphyromonas gingivalis biofilm.
Jeomil CHOI ; Soo Jin KIM ; Sung Jo KIM
The Journal of the Korean Academy of Periodontology 2001;31(4):661-668
No abstract available.
Biofilms*
;
Fusobacterium nucleatum*
;
Fusobacterium*
;
Porphyromonas gingivalis*
;
Porphyromonas*
7.Prior Immunization with Fusobacterium Nucleatum Interferes with Opsonophagocytosis Function of Sera against Porphyromonas Gingivalis.
Jeom Il CHOI ; Melinda A BORRELLO ; Christopher W CUTLER ; Maurice ZAUDERER
The Journal of the Korean Academy of Periodontology 2000;30(1):105-110
No abstract available.
Fusobacterium nucleatum*
;
Fusobacterium*
;
Immunization*
;
Porphyromonas gingivalis*
;
Porphyromonas*
9.Induction of NADPH oxidases and antioxidant proteins by Porphyromonas gingivalis in KB cells.
Min Jeong KIM ; Hyun Ju CHUNG ; Byung Ju PARK ; Hae Ryoung PARK ; Tae Hoon LEE
The Journal of the Korean Academy of Periodontology 2006;36(4):863-878
No abstract available.
Humans
;
KB Cells*
;
NADP*
;
NADPH Oxidase
;
Oxidoreductases*
;
Porphyromonas gingivalis*
;
Porphyromonas*
10.Establishment of a model of intracellular infection on human dental pulp fibroblast by Porphyromonas gingivalisin vitro.
Yu LU ; Shi-gao LUO ; Ding-ming HUANG
West China Journal of Stomatology 2008;26(1):87-93
OBJECTIVETo establish a model of intracellular infection of human dental pulp fibroblast (HDPF) by Porphyromonas gingivalis in vitro.
METHODSHDPF was incubated with live Porphyromonas gingivalis with a multiplicitas of infection (MOI) from 100 for 0.5, 1.0, 2.0 hours. HDPF infected with live Porphyromonas gingivalis were observed by inverted microscope. Metronidazole, Penicillin, Streptomycin were added to the medium to kill any extracellular bacteria. Then the cells were lysed by sterile distilled water, dilutions of the lysates of cells were cultured anaerobically. Colony of invasive bacteria were then observed. HDPF exposed live Porphyromonas gingivalis with a MOI 50 and 100 for 1.0 hour, then cells survival rate were detected by MTT assay.
RESULTSAfter infected with Porphyromonas gingivalis 2.0 hours, cells were eumorphism. Porphyromonas gingivalis of incubation for 1.0, 2.0 hours was invasive cells. MTT assays indicated 99.07% cells survival rate with a MOI from 50 and 74.43% cells survival rate with a MOI from 100.
CONCLUSIONA model of HDPF intracellular infection with Porphyromonas gingivalis was successfully established in vitro.
Dental Pulp ; Fibroblasts ; Humans ; Metronidazole ; Porphyromonas ; Porphyromonas gingivalis
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